amp 945 and amp 946 carbonic anhydrases as novel drugs for gastroduodenal diseases
The a and b adapters are used as priming sites for both amplification
Ngày tải lên :
19/03/2014, 22:32
... Adapters • The A and B adapters are used as priming sites for both amplification and sequencing since their composition is known • The B adapter contains a 5’ biotin tag used for mobilization ... Enzyme beads and packing beads are added Enzyme beads containing sulfurase and luciferase, and packing beads used only to keep the DNA beads in place • Above the wells is a flow channel, passing nucleotides ... and a successive apyrase wash, followed by the next nucleotide.) • As a bi-product of incorporation, DNA polymerase releases a pyrophosphate molecule (PPi) • The sulfurylase enzyme converts the...
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Báo cáo y học: "Central venous O2 saturation and venous-toarterial CO2 difference as complementary tools for goal-directed therapy during high-risk surge" ppsx
Ngày tải lên :
13/08/2014, 21:21
... optimal cut-off was defined as the value associated with the highest sum of sensitivity and specificity (Youden’s index) Analysis was performed using SEM software [32] and significance was set at P ... relationship between an increased PCO2 gap and insufficient flow [39] According to our P(cv-a)CO2 values and the associated trends in both lactate and base excess concentrations (Tables and 4), it can be ... relate to a relatively insufficient flow state, and could be associated with an increased O2 demand and hence increased CO2 production Whether increasing in the CI may be beneficial in this situation...
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foucault and kadan-limit order book as a market for liquidity
Ngày tải lên :
31/10/2014, 12:11
... s sd ES asa m eas re f the w elare of u or f i mpati t trad ersw ho s b mi m ark ord ers en u t et M an s d i ex l svel f u onthe b i -as s y tu es c u i y oc s d k pread as a m eas re ofm ... tti gm g sve pread i provem en et l er) .For thi m tsg arg sreas on the l g ofthe b ook ten sto d ec reas w hentrad ers arri en th d e ' valrate d ec reas As es a c onequ c e the ex te ex ted w ... 1;K i.Asf the i pati t type there are tw o c as : j or m en es R Cas 1: T he i e mpati t type s b m i en u tsa m ark ord er f eac h s h1 + 1;K i i et or j n w hi h c as w e s sc= K c e et Cas :...
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foucault, kadan and kandel-limit order book as a market for liquidity
Ngày tải lên :
31/10/2014, 12:12
... Handa and Schwartz (1996), Harris and Hasbrouck (1996), Kavajecz (1999), Sandås (2000), Hollifield, Miller and Sandås (2001), and Hollifield, Miller, Sandås and Slive (2002) In extant models, traders ... of the game, as well as all the parameters, are assumed to be common knowledge 2.2 Discussion It is worth stressing that we abstract from the effects of asymmetric information and information aggregation ... 3.4 21 See Seppi (1997), Harris (1998), Goldstein and Kavajecz (2000), Christie, Harris, and Kandel (2002), and Kadan (2002) for arguments for and against the reduction in the tick size in various...
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Electrochemical syntheses and self assembly of nanostructure as modified electrodes for polythiophene preparation
Ngày tải lên :
17/09/2015, 17:17
... metallic domains and theories based on conformational structures and conformational defects such as soliton, polaron and bipolaron introduced during doping process[15] 1.2.1 Band Theory The simplest ... aromatic and quinoid As illustrated in Figure 1.3, the aromatic form and the quinoid form of thiophene are not equivalent in energy, the quinoidal form has higher energy than the aromatic form [17, ... molecular orbital (HOMO) and the lowest unoccupied molecular orbital (LUMO) is known as bandgap energy (Eg ) As shown in Figure 1.1, for metals, the VB and CB are overlapped and the intrinsic conductivity...
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báo cáo khoa học: "Gene-expression and network-based analysis reveals a novel role for hsa-mir-9 and drug control over the p38 network in Glioblastoma Multiforme progression" ppsx
Ngày tải lên :
11/08/2014, 12:21
... approach for associating aberrations with disease phenotype and for localizing critical genes [13] MicroRNAs (miRNAs) role in many human diseases is well established, and their ability to act both as ... Gene-expression and network-based analysis reveals a novel role for hsa-mir-9 and drug control over the p38 network in Glioblastoma Multiforme progression Rotem Ben-Hamo1 and Sol Efroni1,* The Mina and Everard ... indicates copy gains and -1 refers to heterozygous deletion A standard threshold for copy number alteration of >0.3 for amplification, and
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Flavor enhancement as a tool for increasing pleasantness and intakeof a snack product among the elderly
Ngày tải lên :
03/04/2013, 21:06
... water before tasting the samples and between samples The samples were coded with three-digit random numbers, and their presentation order was individually randomized for each subject Before evaluating ... aroma sample was perceived to be slightly less pleasant in the second tasting session than in the first one, whereas the pleasantness of the heightened aroma sample increased in the second tasting ... quality of the sample and another group received information about the sample’s healthiness and low fat content A third group served as control, receiving no additional information, except for the list...
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Tài liệu Báo cáo khoa học: Processing, catalytic activity and crystal structures of kumamolisin-As with an engineered active site pptx
Ngày tải lên :
19/02/2014, 07:20
... mature form of this enzyme to clarify the structural basis for the preference of the enzyme for collagen [7] As in kumamolisin, the catalytic triad of kumamolisin -As is formed from Ser278, Glu78, and ... the 19-kDa protein and the wild-type 37-kDa mature form were incubated at pH and 50 °C for 10 and then subjected to SDS ⁄ PAGE, the 19-kDa band disappeared, whereas the 37-kDa band remained (Fig ... buffer at °C for 24 h (right) Lanes b, the nicked precursor was incubated at pH 7.0 and °C for indicated periods of time Lane c, the nicked precursor was incubated at pH 7.0 and °C for 24 h and then...
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Tài liệu Báo cáo khoa học: Infrared spectroscopy as a tool for discrimination between sensitive and multiresistant K562 cells doc
Ngày tải lên :
21/02/2014, 03:20
... same density of cells and then harvested in the same phase of culture growth (exponential) for IR measurement For harvesting, cells were centrifuged at 300 g and the pellet washed twice in a solution ... assigned to the asymmetric stretching of CH3, and the band at 2873 cm)1 to its symmetric mode The bands at 2926 and 2853 cm)1 can be assigned to an asymmetric and symmetric stretching mode of CH2, respectively ... Benedetti et al assigned the shoulders present at 1117 and 1020 cm)1 to RNA and DNA, respectively [18] Classification by LDA Classification by LDA on spectra reduced by PCA PCA was performed on the...
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Tài liệu NON-WOOD PLANTS AS RAW MATERIAL FOR PULP AND PAPER pptx
Ngày tải lên :
22/02/2014, 09:20
... analysis of variance was done for DM yield, stem proportion and number of stems m-2, content of crude fibre, ash and silica as well as for pulping characteristics Harvest year was used as a repeated ... and production costs): MTT, University of Helsinki and Work Efficiency Association 11 AGRICULTURAL AND FOOD SCIENCE IN FINLAND Saijonkari-Pahkala, K Non-wood plants as raw material for pulp and ... such as grasses and leaf fibre plants, belong to the class Monocotyledonae and bast fibre and fruit fibre plants are dicotyledons (IlvessaloPfäffli 1995) Promising new non-wood species for fibre...
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Báo cáo khoa học: Molecular cloning and characterization of two soybean protein disulfide isomerases as molecular chaperones for seed storage proteins doc
Ngày tải lên :
07/03/2014, 05:20
... Biotech, Kyoto, Japan) RNaseA refolding assay PDI activity was assayed by the measurement of RNase activity produced through the regeneration of the active form from reduced RNaseA as described previously ... of reduced and denatured RNaseA, was examined The specific activities of GmPDIL-1 and GmPDIL-2 were 472 and 300 mmol RNaseAÆmin)1Æmol)1, respectively (Fig 2A) Several mammalian and yeast PDI family ... region between b and b¢ in GmPDIL-1, and between b and b¢ and b¢ and a¢ in GmPDIL-2, the structure of these regions may be protease resistant The activity of recombinant GmPDIL-1 and GmPDIL-2, which...
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American Negro Slavery A Survey of the Supply, Employment and Control of Negro Labor as Determined by the Plantation Regime docx
Ngày tải lên :
08/03/2014, 00:20
... considered as champions of rival faiths, the pagan blacks came increasingly to be reckoned as having no religion and therefore as a mere passive element ready for christianization As early as 1510, ... agriculture, was classed as domestic service and assigned to the women for performance The wife, bought with a price at the time of marriage, was virtually a slave; her husband her master Now one ... authorization was promptly given for a supply of bozal negroes CHAPTER I 10 [Footnote 12: Las Casas, Historio de las Indias (Madrid, 1875, 1876); Arthur Helps, Life of Las Casas (London, 1873); Saco,...
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Báo cáo khoa học: The oxidative effect of bacterial lipopolysaccharide on native and cross-linked human hemoglobin as a function of the structure of the lipopolysaccharide A comparison of the effects of smooth and rough lipopolysaccharide ppt
Ngày tải lên :
08/03/2014, 10:20
... increased as the pH was lowered over the range from pH 9.0–5.8 (data not shown) As was observed previously by others [5], the reaction is biphasic at pH 7.4 and below, with an initial fast phase ... coli was a lag phase of 10 minutes both in the presence and absence of EDTA The lag phase was followed by a very rapid second phase only in the absence of EDTA Significantly, this lag phase was not ... by a slower second phase over a wide pH range from 5.3 to Tsuruga and Shikama [21] confirmed that the fast phase of oxidation was due to the a chains and the slow phase was due to the b chains...
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Báo cáo khoa học: Molecular characterization of recombinant mouse adenosine kinase and evaluation as a target for protein phosphorylation potx
Ngày tải lên :
16/03/2014, 18:20
... 271) PKA was purified from bovine heart as previously described [11] PKG and cGMP were purchased from Promega; MAPK, CaMKII, and calmodulin from Upstate; and CK1, CK2, and Cdk1 from New England Biolabs ... inhibitor-1 and DARPP-32 were generated as previously described [13,14] Recombinant tyrosine hydroxylase was kindly provided by P Fitzpatrick and C Daubner, Texas A&M University Purified histone H1 and ... in electrophoresis buffer, pH 3.5, and 10% of the total volume was set aside for amino acid analysis The remainder of the sample was spotted on a TLC plate for one-dimensional electrophoresis...
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Báo cáo khoa học: Hematopoietic differentiation from human ESCs as a model for developmental studies and future clinical translations Invited review following the FEBS Anniversary Prize received on 5 July 2009 at the 34th FEBS Congress in Prague docx
Ngày tải lên :
22/03/2014, 17:20
... technology as well as for a complete evaluation of the possible consequences of use of hiPSCs for drug screening, as disease models and for medical purposes Conclusions The use of stem cells for the treatment ... hiPSCs have opened new avenues for creating in vitro disease models that can be used to help understand the pathology of many genetic diseases and to design new drugs They are also considered ... groups As early as 1970, Moore and Metcalf [31] demonstrated that primitive hematopoiesis starts with the formation of yolk sac blood islands in mouse embryos, and later studies by Medvinsky and...
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Báo cáo khoa học: Mechanisms of amyloid fibril self-assembly and inhibition Model short peptides as a key research tool pptx
Ngày tải lên :
23/03/2014, 11:20
... fibrils is associated with major human diseases and has a clear physiological role in micro-organisms, the precise mechanism of its formation is not fully understood As most amyloid-related diseases ... molecular information required for the molecular recognition and self-assembly mediating the formation of amyloid fibrils The small size of the peptides has reduced the complexity of the amyloid formation ... self-assembly must occur to enable the formation of such well-ordered, supramolecular structures Short peptides as models for amyloid formation As a result of the complexity and enormous structural space...
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![Báo cáo khoa học: Properties and significance of apoFNR as a second form of air-inactivated [4Fe-4S]ÆFNR of Escherichia coli pot](https://media.store123doc.com/images/document/14/rc/at/medium_ata1395562826.jpg)
Báo cáo khoa học: Properties and significance of apoFNR as a second form of air-inactivated [4Fe-4S]ÆFNR of Escherichia coli pot
Ngày tải lên :
23/03/2014, 15:20
... the protein was precipitated and washed carefully with methanol–chloroform [31] The protein concentration was determined using the Bradford assay [32] and the radioactivity was measured in a scintillation ... time-points, samples were removed and denatured in a solution of GnHCl (4 M) and [14C]iodoacetate (12 mM and 43 BqÆ mmol)1 iodoacetate) The protein was precipitated and washed with methanol + chloroform ... carboxymethylation, trypsin and AspN digestion and reversed-phase chromatography The sample buffer was changed by gel filtration on a Nap10 column and then subjected to trypsin digestion The digest was separated...
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