... activity of PAI-1 variants towards uPA The most common amino- acidcompositionof the buried polar cluster (positions 53/56/334) in serpins is S/S/H The composition S/S/Q is identical to that of alaserpin, ... network involves the side chains of the amino acids in positions 53 and 56 in a helix B, 186 in b strand 3A, and position 334 in b strand 5A (Fig 1; the numbering ofamino acids in PAI-1 is according ... combination ofamino acids in these positions resulted in an accelerated latency transition Substitution of the Lys residue in position 335 counteracted the accelerating effect of some of the substitutions...
... amino acids (i.e Q, N, S, and T) and an increase in charged amino- acid residues (i.e K, E, and R) in hyperthermophilic proteins As S and T can catalyze the deamination and backbone cleavage of ... One unit of isomerase activity is defined as the amount of enzyme that produces mmol of product per under the assay conditions Activation of apo-BLXI by metals To examine the effect of bivalent ... the presence of Co2þ or Mn2þ are interesting With a similar inactivation mechanism, Ea of inactivation of T thermosulfurigenes XI is only 490 kJ·mol21 in the presence of Table Effect of metals on...
... sequence of the open reading frame (ORF) of their cDNAs and the corresponding aa sequences, in Table Summary of the purification of DNases I from the pancreases of three species of snake The results of ... Comparison of the three primary structures of deoxyribonuclease isolated from bovine, ovine, and porcine pancreas Derivation of the aminoacid sequence of ovine DNase and revision of the previously ... presence of Val67 is one of the essential factors responsible for the actin-binding capacity of these snake DNases I Tissue distribution of snake DNases I Fig Electrophoretic patterns of snake...
... macrophages suggested that the lipid compositionof lipoproteins can alter the conformation of the protein [40] Thus, the differential uptake of CRLPs of different fatty acidcomposition by macrophages ... differential rates of uptake of CRLPs of different fatty acid compositions by macrophages observed in the present study is due to differences in the oxidative state of the particles Evaluation of the lipid ... particular type of fatty acid predominating in the oil, but also contained a complex mixture of other fatty acids, as occurs in vivo As might be expected, the fatty acidcompositionof CRLPs containing...
... changed by any of the four types of CRLPs Effect of the fatty acidcompositionof CRLPs on COX-2 mRNA expression The effect of CRLPs of varying fatty acidcomposition on expression of COX-2, an ... acidcompositionof the particles modulates this effect Earlier studies on the effects of free fatty acids on NF-jB activity in macrophages have also suggested that different types of fatty acids ... mixture of fatty acids which reflects the compositionof the parent oils and of physiological CMR derived from them [28] The triacylglycerol ⁄ total cholesterol ratio in the four types of CRLPs...
... 615.8) The difference of 921.4 Da was indicative of a deletion of about eight aminoacid residues RP-HPLC elution patterns of tryptic hydrolysates of a B and H showed the absence of one peak in a ... masses of a and CNBr peptides of a were measured -Cn sl -Cn si MALDI-MS First, vL of the sample was mixed with !iL of the matrix -Cn; sl (sinapinic acid for a 4-hydroxy-a-cyano-cinnamic acid for ... and zebus less clear !21, 24, 28) The lack of data on the genetic polymorphism ofmilkproteins in African cattle is unfortunate because the diversity of these populations is exceptionally high,...
... PMI of 24 h at day by excluding the afternoon milking In the rest of the study milking was performed twice daily Samples of approximately 40 ml of composite cow milk were taken at every milking ... and compositionofmilk [15] Besides the effect on the milk synthesis, elevated SCC has a direct negative effect on milk quality and shelf life [16] In the milk quality control at the dairy, milk ... the cell content ofmilk but also the milkcomposition and yield Milking cows, regularly, just once a day appears to result in reduced milk yield and lactose content, compared to milking two times...
... ratio of the amount of p24 to that of p55 of each virus is shown at the bottom A representative of three independent experiments is shown Figure Structural model of the N-terminal domain of HIV-1 ... insight into the effects of the G-to-E single aminoacid substitution at the 116th position of the CA on its three-dimensional (3-D) structure, the 3-D model of the N-terminus of the CA was constructed ... aspartic acid) A single aminoacid substitution in CA rescued impaired replicative capability in human cells To determine whether the single aminoacid substitution at the 116th position of the...
... indispensable amino acids that the fish cannot synthesize and (ii) a supply of either dispensable amino acids or sufficient amino nitrogen to enable fish to synthesize them The concept of balance amino acids ... 2013) Lacking of information on the amino acids requirement of striped catfish is therefore the main constrain on utilization of supplemental amino acids and thereby development of more sustainable ... reduced either lacking or excessing any amino acids 3.5 ADC of EAA of ingredients The ADC of essential amino acids in ingredient were shown in Table Table ADC (%) of EAA (FM-fishmeal, SB-soybean, WB-wheat...
... identical contiguous amino acids For proteins from 39 to 79 amino acids in length, removal of each aminoacid increased the number of pairs sharing only identical contiguous amino acids by less than ... in the slope of the line at the 39 -amino- acid length When the shorter protein in the pair was less than 39 amino acids long, each amino- acid reduction resulted in >18 new pairs ofproteins sharing ... under 80 amino acids Relationship between amino- acid length and the cumulative number of 8-mer-only matches produced Lines depict the linear regression ofproteins from 29 to 40 amino acids and...
... version of this paper Additional data file is a music clip of the human ThyA protein based on the single note assignment of one aminoacid per musical note Additional data file is a music clip of ... portion of this sequence fixed to a 20-note range (2.5 octaves), where each aminoacid was initially assigned to a unique note One way to improve the musicality is to express each aminoacid as ... reduced-note assignment Certain similar amino acids were paired and assigned a three-note chord (triad) starting an octave below middle C Each member of the amino- acid pair was distinguished from the...
... to 21% of mammalian proteins and 13% of chicken proteins contained some kind of tandem AAR and 12% of mammalian proteins and 9% of chicken proteins contained some kind of C4 repeat; 4.6% of IURs ... significant overrepresentation of runs of a single aminoacidof length 4; they therefore differ from tandem repeats of that aminoacid because they fall below the definition of a tandem repeat Throughout ... regions of disorder [40,41] In this dataset we find 34% ofproteins to contain IURs of length > 50 and 85% to contain an IUR of length > 10 These regions are thought to form flexible regions of proteins...
... AND DISCUSSION 3.1 Fatty acid compositions of instant noodles Table shows fatty acidcompositionof instant noodles analyzed Palmitic acid was the most abundant fatty acid in all samples; its ... 2009) Fatty acidcomposition including trans fatty acids content of selected Vietnamese instant noodles Table1 Fatty acids compositionof selected Vietnamese Instant noodles Fatty acid C14:0 Brands ... The presence of high amounts of palmitic acid indicated the presence of palm oil Oleic acid was the next main fatty acid, that accounts for from 32% to 36%, followed by linoleic acid with 814...
... somewhat higher content of palmitic and lower contents of linoleic and R-linoleic acids The aril also contains a significant, but varying, amount of stearic acid and small amounts of cis-vaccenic, myristic, ... principal fatty acid components of such fats are palmitic, oleic, and linoleic acids Gac is somewhat unusual in having a higher proportion of linoleic acid, and given the similar percentage of the three ... shows data on FAME composition (as % total FAME) in the aril of each of the two fruit, as well as average values Gac aril has high concentrations of oleic, palmitic, and linoleic acids These data...
... Association of Feed Control Officials, a metal aminoacid chelate is lithe product resulting from the reaction of a metal ion from a soluble salt with amino acids with a mole ratio of one mole of metal ... 47 76 xx Contents EVALUATION OF THE NUTRITIONAL EFFICIENCY OFAMINOACID CHELATES 86 Silvano Maletto and Germano Cagliero AN ASSESSMENT OF LONG TERM FEEDING OFAMINOACID CHELATES 106 Robert B ... of a specific mineral in the feed; (b) The Roles ofAminoAcid Chelates in Animal Nutrition an imbalance of another mineral or nutrient including certain vitamins, amino acids and fats, any of...
... [14C]-labelled amino acids or [32P]-labelled tRNA Assay method Aminoacylation of transcript tRNA with [14C]-labelled aminoacid Aminoacylation of [32P]-labelled transcript tRNA Arg Arg Cav Cav Source of ... comparison ofaminoacid utilization by the plant arginyl-tRNA synthetases The aminoacylation level attained in the presence of L-arginine was compared to that in the presence of mM of the analogue ... be aminoacylated with arginine to a level of approximately 0.05 pmol aminoacid ⁄ pmol tRNA The corresponding soybean transcript had an arginine acceptance level of approximately 0.1 pmol amino...
... and S Janecek Origin of rBAT and 4F2hc within the GH13 a-amylase family Fig The CSRs of the hcHAT proteins and the GH13 a-amylase family members A list of the abbreviations ofproteins and enzymes ... available sequences of hcHATs and hcHAT-like proteins were collected (Table 1) using the aminoacid sequences of human 4F2hc (GenBank accession number M21904) [24] and rBAT (M95548) [25] proteins as ... can be traced in the sequences of hcHAT1 and hcHAT2 groups as well as of the ATG proteins (Fig 2), indicating evolutionary relationships of all these enzymes and proteins and hinting at their...
... proline, aspartic acid, serine or alanine Simple and double FNR mutants ofamino acids C266 and L268 were obtained and characterized It was observed that alteration of the aminoacid volume decreases ... design of the mutants was intended to preserve the aminoacid character and to modify only the relative volume of their R groups The expression of the FNR mutants as soluble cytosolic proteins ... efficiencies of wild-type and mutant FNRs plotted as a function of different aminoacid physicochemical properties The catalytic efficiencies of wild-type and mutant FNRs from Table (percentage of the...
... of Glu, glycosylation of Ser or Thr, bromination of Trp, sulfation of Tyr, cyclization of N-terminal Gln, and epimerization of several different residues It is quite rare for such a variety of ... structure of conomarphin was similar to those of other conotoxin cDNAs The cDNA-encoded precursor of conomarphin consisted of a conserved M-superfamily signal peptide of 25 residues, a proregion of ... physiological role of specific Pro hydroxylation in conotoxins is still elusive 1982 The epimerized d-Phe13 is another striking feature of conomarphin The l -amino acid to d -amino acid epimerization...
... for individual aminoacid contents are of the same order of magnitude as those of the total aminoacid contents for glutamine (C), glutamic acid (D), asparagine (E), aspartic acid (F), alanine ... Authors Journal compilation ê 2008 FEBS 3195 Aminoacid metabolism and translocation M.-H Valadier et al Table Aminoacidcomposition in leaves, and aminoacid percentage ratio in the phloem exudates ... 21.90 1.93 (dark) The standard errors for the individual aminoacid contents are of the same order of magnitude as those of the total aminoacid contents Phloem exudates and xylem sap were collected...