amebiasis worms trichomonas vaginalis and vincent´s angina

... senile cataract (79 female, 76 male) and the control one consisted of 155 of cases’ close relatives (79 female, 76 male) Mean age and standard deviation of patients and control group were 61.96±14.5, ... range of patients and controls were 44-85 year and 43-86 year respectively Table shows serum Na+, K+ of case and control groups, with a significant difference in serum + Na of case and control ones ... hypertension and any other systemic disease Then positive ones of both groups were excluded from the study Case and control groups were both examined by an expert ophthalmologist and routine ophthalmic...

Ngày tải lên: 03/11/2012, 09:49

... in 20 and 40% dimethylsulfoxide were )6.86 and )13.02 kJÆmol)1; with Na+ the Ó FEBS 2003 ´ 2382 L Ramı´ rez-Silva and J Oria-Hernandez (Eur J Biochem 270) Fig Competition between Na+ or K+ and ... kinase with and without cations and various concentrations of dimethylsulfoxide Differences in the intrinsic fluorescence of pyruvate kinase incubated with and without ligands (K+, Mg2+ and phosphoenolpyruvate) ... presence of 30 and 40% dimethylsulfoxide and in the absence of ligands, pyruvate kinase exhibited the active conformation Moreover, in the presence of dimethylsulfoxide and ligands, pyruvate...

Ngày tải lên: 08/03/2014, 02:20

... (DP0559396 to G M Nicholson and G F King, and DP0774245 to G F King) and Australian Postgraduate Awards to S J Gunning and M J Windley Requests for samples of J-ACTX-Hv1c and ⁄ or the expression ... channels are located on the b-strands Pharmacophore and gasket residues are shown in green and yellow, respectively (F) Overlay of the structure of J-ACTX-Hv1c (red) and ChTx (Protein Data Bank code ... mammalian Slo channels (IC50 values of 36 and 7.4 nm for hSlo and mSlo, respectively) more potently than insect Slo channels (IC50 values of 158 nm and > lm for pSlo and dSlo, respectively) [14,43] In...

Ngày tải lên: 16/03/2014, 06:20

... extraction and pool preparation Blood was sampled from paternal grand-parents, sire, dams and all progeny Individual extraction of high molecular weight DNA was performed according to standard procedures ... uropygial gland, U , lavander plumage color, LAV , and ametapodia, M P , mutations would be the last one not to be connected with the molecular map The connection between molecular and classical ... 800 cM The Z and W chromosomes were excluded from the analysis because N A and P O loci are not sex-linked Then, individual typings with a small subset of markers were performed on grandparents,...

Ngày tải lên: 09/08/2014, 18:21

... Alexander G Obukhov, and Michael Sturek for their invaluable direction and counsel This work was supported by AHA grants 10PRE4230035 (ZCB) and NIH grants HL092245 (JDT) and HL062552 (MS) iii ... investigated candidates include components of the reninangiotensin-aldosterone system (RAAS) Notwithstanding the systemic and renal influences of these hormones, angiotensin II (AngII) and aldosterone ... normal cardiac output and blood pressure MetS impairs the ability of the coronary circulation to regulate vascular resistance and balance myocardial oxygen supply and demand (39; 269; 329) Coronary...

Ngày tải lên: 24/08/2014, 11:10

... Water and Environment Technology, Vol 7, No 2, 2009 concentration and a high ESP (Quirk and Schofield, 1955) In recent years, the air, water and soil in China have been polluted by economic and ... the gypsum, and to examine the transport of solutes in alkali soil reclamation The inner hydraulic conductivity and the transport of salts such as cations (Na+, Ca2+, Mg2+ and K+) and Cl- in a ... application rates of 0.5 wt% and 1.0 wt% Alkali soil and gypsum were mixed uniformly and were packed in an acrylic ring with an outer diameter of 60 mm, inner diameter of 50 mm, and height of 20 mm at...

Ngày tải lên: 05/09/2013, 09:38

... resistance and avoid the possibility of deformation 4.2 Gate location The best position of the gate is found by trial and error and different positions for the gate are suggested and visualise ... stay between 2.5 and 5% for semi-crystalline thermoplastics and between and 10% for amorphous thermoplastics 5.3 Cavity channels positioning Different solutions for the core and the cavity cooling ... most manufacturing fields, production time and costs (lead and lag) are strongly correlated The longer it takes to produce parts the more are the costs, and with injection moulding production industries...

Ngày tải lên: 12/02/2014, 22:20

... activated with Mg, Cu, Na and Si and studied the dependence of the glow curve and the TL intensity on thermal treatment and the concentration of the dopants The LiF:Mg,Ti and LiF:Mg,Cu,P powders ... CuCl2 , and (NH4 )2 H2PO4 with required concentration were added to LiF precipitated by reaction (1) The material obtained was washed, dried, and was annealing in an oven at 700 o C for hour, and ... incorporated The material precipitated was filtered, washed, dried, and was subjected to thermal treatment in an oven at 640o C for hour, and then was annealed at 400o C for hour LiF:Mg,Cu,P powders...

Ngày tải lên: 13/02/2014, 04:20

... proteins and a protein molecular weight marker Protein bands were stained with Coomassie blue For both, GST-N and GST-C34, an hCaM band is only seen in the presence of Ca2+ (lanes 5, and 9, 10) ... calmodulin-binding domain C2 (BD-C2, Figs and 3C) The other two regions contain a potential binding site in the N-terminus (BD-N; Figs and 3A) and in the C-terminus (BD-C1; Figs and 3B) There are additional ... CaM-EF-hand mutants testing for interaction with the functional C- and N-terminal binding sites of hEAG1 channels highlighted the importance of Ca2+-binding ability of EF-hand motifs and in CaM...

Ngày tải lên: 07/03/2014, 12:20

... Effects of a1-PTH and PIN-a on NG108-15 cells In (A) and (B), the cells were stained with lM FM1-43 dye for 30 min, and thereafter abundantly washed with dye-free solution before imaging and the addition ... blebbing and disorganization and disarray of cytoskeletal actin, may represent apoptotic events involved in a1-PTH cytotoxicity Experimental procedures Extraction and purification of PIN-a and a1-PTH ... PINs Separate pools of the PTH-containing and PIN-containing crude fractions were dialyzed against deionized water and freeze-dried, and a1-PTH, a2-PTH and b-PTH were separated (at room temperature)...

Ngày tải lên: 07/03/2014, 12:20

... intensity and exposure to solvent in a number of Trp residues (60d, 96, 148, 207, and 215) [12] In particular, Trp207 and Trp29 residues, located at the boundary between the A- and B-chains and interacting ... in vitro and its hemorrhagic phenotype in vivo Results and Discussion Effect of pH on thrombin catalytic activity and inhibition The pH-dependent steady-state amidase activities of WT and K9-deleted ... the W60d side chain (S2 site) and shifting of W215 (S3) Functional and computational data show that the catalytic cycle and efficient interaction with substrates and natural inhibitors by DK9...

Ngày tải lên: 07/03/2014, 12:20

... program LIGAND (Elsevier Biosoft) using Ôcold saturationÕ or Ôhot saturationÕ analysis The kinetic data for ligand association and dissociation rates were subjected to analysis by LIGAND, using ... LIGAND (see Experimental procedures) The dissociation constants (Kd) were 0.57 ± 0.2 nM and 6.5 ± 1.4 nM and the maximum number of binding sites (Bmax) were 1.24 ± 0.17 pmolÆmg protein)1 and ... [32] The synaptosomes, which were concentrated at the 12 and 16% Ficoll gradient interface, were washed and aliquoted into Eppendorf tubes and stored at )80 °C Before experiments, the synaptosomes...

Ngày tải lên: 08/03/2014, 16:20

... the Materials and methods and separated by SDS/PAGE Membrane fractions for A–D and G were prepared using a detergent free, sodium carbonate-based procedure [11] Membranes for E and F were prepared ... re-extracted with 1% Triton X-100 pelleted and the supernatant and pellets were subjected to SDS/PAGE and immunoblotting as described in the Materials and methods’ Lane 1, untreated fraction 5, ... distribution of the Na+/H+ exchanger and caveolin The Na+/H+ exchanger (Fig 4C) was almost absent from fraction and was present in much greater amounts in fractions and and in fraction 12 The distribution...

Ngày tải lên: 08/03/2014, 16:20

... connecting fragment (IIIa) and transmembrane helices IV and VIII comprise the most highly conserved areas of the molecule, and within these areas D203, Y229, N373, G377, S382 and R389 have a profound ... N373D and double mutant D203N/N373D, pSK-GAB [10] and pSKGABD203N [11] served as template, respectively The purified PCR fragments were used as template for a second PCR using primers Kpn2Ifor and ... fragment was subsequently digested with Kpn2I and Bst1107I and cloned into plasmid pSK-GAB, digested with the same enzymes, yielding plasmids pSK-GABN373D and pSK-GABD203NN373D Purification of oxaloacetate...

Ngày tải lên: 08/03/2014, 23:20

... was the same at t ¼ and h This indicates that the 14 C sucrose was trapped by loading and resealing and did not leak out of the ghost cells The ghost cells were incubated with and without modifiers ... were prepared as described in the methods section and incubated at 37 °C, 10 lL samples taken at t ¼ and t ¼ h and assayed Error bars represent standard deviation 2610 B K Derham et al (Eur J Biochem ... heart disease, hypertension and cataract Altered Na+ and K+ concentrations are observed in many forms of human cataract and correlate with increasing lens colour and with cortical opacification...

Ngày tải lên: 17/03/2014, 03:20

... both hydrophobic forces and hydrogen bonding between Tyr35 (dyad) and the aromatic cluster consisting of Trp344, Trp345* and Tyr355* (Fig 7B, legend), and between Ile28 and Val361 The Lys26 (dyad) ... and the filtrate was precipitated and washed twice with cold diethyl ether After centrifugation at 2800 g for 12 min, the supernatant was discarded and the crude peptide was dissolved in H2O and ... apamin and was shown to be less than 8% of the total binding Electrophysiology Oocyte preparation Xenopus laevis oocytes (stages V and VI) were recovered and prepared for cRNA injection and electrophysiological...

Ngày tải lên: 17/03/2014, 10:20

... maximal inactivation and the slope factor for other channels were: –74.0 ± 1.7 mV* and 7.6 ± 0,4 mV*** for (R180C) –76.7 ± 1.5*** mV and 6.6 ± 0.2** mV (R834C) and –75.0 ± 1.5 mV** and 6.4 ± 0.3 mV* ... smegmatis for tandem expression of DrrA and DrrB and characterized them as an ATP-binding protein and an integral membrane protein respectively In both the expression systems DrrA and DrrB behave ... fermentations, for instance, in the dairy and wine making industries Decarboxylation of acids can improve the hygienic quality and the taste and texture of cheese and wine and, as such, give added value...

Ngày tải lên: 23/03/2014, 15:20

... [27] In view of the multiplicity of the stimuli and receptors that have been shown to regulate cardiac hypertrophy, and because of the evident overlap and superficial similarities of the signal pathways ... 2436 Z Xie and A Askari (Eur J Biochem 269) Fig The signal transducing function of Na+ +-ATPase and its consequences in cardiac myocytes Two pools of the enzyme, one pumping ions /K and the other ... changes in [Na+]i, [K+]i, and [Ca2+]i that may occur These activated pathways, the resulting increase in ROS, and the concomitant increase in [Ca2+]i lead to activations of NF-jB and AP-1, transcriptional...

Ngày tải lên: 24/03/2014, 00:21

... curve The protein standards were BSA (67 kDa), ovalbumin (43 kDa), pGEX vector GST (27 kDa) and ribonuclease A (13.7 kDa) The void volume V0 and the total column volume Vt were 4.5 and 70 mL, respectively, ... ratios of 0.70 and 0.74, respectively, and the corresponding reversal potentials (+9.5 and +7.8 mV) are indicated calculated from contiguous 60 s recordings for each condition, and typical recordings ... cleaved by thrombin, and the enzyme and the free tag were scavenged using benzamidine–Sepharose 4B beads and Ni2+-NTA resin (Amersham, Chalfont St Giles, UK) CLIC5 aggregated and precipitated in...

Ngày tải lên: 30/03/2014, 04:20