allocation models for chemical defense
Marine Chemical Ecology - Chapter 14 pptx
Ngày tải lên :
12/08/2014, 00:21
... identified chemically, and genes coding for a large number of chemoreceptors for as yet unidentified odorants certainly exist in the genomes of marine organisms As research in molecular aspects of chemical ... the source of the noxious chemical Using behavioral and genetic assays, Adler13 concluded that detection of chemical signals was by means of receptors that recognized the chemical structures of ... 5:25 AM 468 Marine Chemical Ecology Physiological, Biochemical, and Molecular Studies Building upon the behavioral observations of glutathione-initiated feeding in Hydra, Rushforth and colleagues39,40...
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Báo cáo khoa học: The essential tyrosine-containing loop conformation and the role of the C-terminal multi-helix region in eukaryotic phenylalanine ammonia-lyases docx
Ngày tải lên :
07/03/2014, 12:20
... been used as templates for homology modeling resulting in raw models with loop-in (HALbased models) and the loop-out (PAL-based models) conformations of the Tyr-loop region For modeling the whole ... bacterial PALs For further refinements, the HAL-based models were chosen for both bacterial PALs but the 256–304 parts in both models were replaced by the corresponding part from the PAL-based models ... inactivation of PAL by enforcing the Tyr-loop-out conformation which is catalytically inactive and more sensitive to degradation The presence of conformationally stable Tyr-loop-out forms in PAL preparations...
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Báo cáo khoa học: Analysis of the regulatory motifs in eukaryotic initiation factor 4E-binding protein 1 pot
Ngày tải lên :
16/03/2014, 06:20
... serum for 16 h and then treated with 100 nM insulin for 25 min, where indicated (see Experimental procedures for details) Samples were analysed with the indicated phosphospecific antibody for Ser64 ... 17 amino acids [compare the signal for full-length 4E-BP1 (1– 117) with that for the ‘18–117’ variant] and sections of the C-terminal half of 4E-BP1 [compare, for example, the 4E-BP1 (98–117) variant ... for simply by the loss of the RAIP motif We therefore also tested the binding of raptor to full-length 4E-BP1 and to the RAIP ⁄ AAAA variant A marked and reproducible 2188 decrease was seen for...
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Báo cáo khoa học: Analysis and biological relevance of advanced glycation end-products of DNA in eukaryotic cells ppt
Ngày tải lên :
30/03/2014, 04:20
... activity after transformation of a CEdG-modified vector pGL3 Control into HEK 293 T cells For CEdG formation, 0.1 lgÆlL)1 plasmid was incubated with 100 lM (A), mM (B) and mM (C) DHA for 0, 3, and h ... to measure the DNA glycation rate in cell models In this study, we developed a liquid chromatography (LC)-MS ⁄ MS method for the analysis of the CEdG formation rate in genomic DNA of cultured ... negative ionization for Fig Restriction digests of CEdG-modified and unmodified pGL3 Control vector For the introduction of CEdG adducts, 0.1 lgÆlL)1 plasmid was incubated with mM DHA for h at 70 °C...
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expression of recombinant genes in eukaryotic systems
Ngày tải lên :
11/04/2014, 09:41
... same format as used for the PCR reactions (microtiter plates, either 96 or 384 well) Computer scripts can be written that automate the process of primer selection for groups of sequences or for ... intended for subsequent gene amplification experiments, primary selection can be performed by selecting for growth in MEM Alpha medium depleted of nucleosides [MEM Alpha ( -)], i.e., for dhfr ... temperatures, and protocols usually call for the fragmentation of the labeled material before hybridization to reduce the amount of secondary structure that forms at the lower temperatures The microarrays...
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Medsoft, deciphering principles of transcription regulation in eukaryotic genomes
Ngày tải lên :
25/03/2015, 09:45
... transcription regulation Recent biochemical studies indicate that the TFs support basal transcription and perform lots of the catalytic functions required for initiation Coactivators with the ... data on basal factor mechanism and have been essential for evaluating the validity and ramifications of biochemical and functional studies performed in mammalian systems [15] Here we argue two problems: ... activators/repressors and enhancers/silencers The former is made up of proteins binding to DNA sequences and the latter is formed of DNA sequences Transcription regulation is therefore commonly controlled by two...
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Báo cáo y học: "Growth of Microorganisms in Total Parenteral Nutrition Solutions Without Lipid"
Ngày tải lên :
26/10/2012, 09:39
... before inoculating After 24-hour incubation at 37ºC, the number of colony forming units (CFU) of each microorganism was counted for each plate The mean CFU of duplicate data was calculated for ... results for each species 45 marcescens (Figure 3) and B cereus (Figure 4) also did not increase for 48 hours at pH5.5 and pH6.0; furthermore, these strains did not increase even at pH6.5 for 24 ... increase or decrease in NP1 for 48 hours (Figure 1b), and there was no significant difference in the mean values of the strains All strains of B cereus did not increase in NP1 for 48 hours (Figure 1c),...
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Inactivation of microorganisms in untreated water by a continuous flow system with supercritical CO2 bubbling
Ngày tải lên :
05/09/2013, 09:38
... 37°C for 48 hr Based on the presence of gas in the small glass tube, the most probable number (MPN) was calculated and the coliform bacterial count was determined The detection limit of the coliform ... Pharmaceutical Co., Ltd., Tokyo, Japan) and aerobically incubated at 37°C for 48 hr, and the colony-forming units (CFU) were counted The coliform and total bacterial counts were expressed in MPN/100ml and ... performed in triplicate - 243 - Journal of Water and Environment Technology, Vol 7, No 4, 2009 Electron microscopy E coli cells before and after SC-CO2 treatment were prefixed with 2% paraformaldehyde...
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Evaluation of the Characteristics of Microorganisms that Contribute to Denitrification in the Paddy Drainage Treatment Apparatus by Quinone Composition Measurement
Ngày tải lên :
05/09/2013, 10:15
... factor loading values Content of these quinones were 5.1 - 15.7% for Q-8, 3.6 13.7% for Q-9, less than 2% for MK-9(H6), and less than 1% for MK-11(H2) The contributions of MK-9(H2) and MK-11(H2) were ... especially appears to be the key factor for denitrification in the paddy Quinone composition provides information for the evaluation of the biological potential for denitrification in paddies The ... structures exist based on biological, biochemical and chemical analyses Quinone profiles based on quinone composition in microorganisms provide important information on the community structure of...
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Microorganisms Associated with Volatile Organic Compound Production in Spoilt Mango Fruits docx
Ngày tải lên :
05/03/2014, 20:20
... ml/min The oven temperature was programmed at 60 oC for 5min, followed by an increase (held for min), and finally at 100C/min to 280 oC (held for 10 min) The temperature of the FID was set to 250 ... storage after further validation and the volatile organic compound could form the basis for constructing a metabolomics database for Nigeria References Bódalo, A.; Bastida, J.; Máximo, M.F.; Hidalgo ... room temperature for days and was subculture continuously to obtain pure culture Identification of bacteria and fungi The bacteria isolate were identified following series of biochemical test as...
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Báo cáo khoa học: Eukaryotic class 1 translation termination factor eRF1 ) the NMR structure and dynamics of the middle domain involved in triggering ribosome-dependent peptidyl-tRNA hydrolysis pptx
Ngày tải lên :
07/03/2014, 05:20
... and should therefore be characterized in detail The selection of a representative conformation for the GGQ loop (residues 177–188) was derived from an analysis of all the conformations found ... comparative sequence and structure information for ribosomal, intron, and other RNAs BMC Bioinformatics 3, Jeffrey GA (1997) An Introduction to Hydrogen Bonding Oxford University Press, New York, ... eRF1 For most of the protein residues, the number of NOEs per residue is between 20 and 40; however, this number is significantly lower for residues 178–184, which are near the GGQ motif, and for...
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Báo cáo khoa học: Two functionally redundant isoforms of Drosophila melanogaster eukaryotic initiation factor 4B are involved in cap-dependent translation, cell survival, and proliferation doc
Ngày tải lên :
07/03/2014, 15:20
... thirdinstar larvae for imaginal disc analysis or until adulthood for phenotypic analysis Eye imaginal discs were dissected in · NaCl/Pi, fixed for 20 in 6% (v/v) formaldehyde/ · NaCl/Pi, blocked for h in ... respective 3¢-UTRs (62 nucleotides for the long and 409 nucleotides for the short form) The ORFs predict a Dm-eIF4B-L isoform of 459 amino acids (52.2 kDa) and a Dm-eIF4B-S isoform of 390 amino acids (44.2 ... Annelies Zechel for excellent technical assistance, Jose Alcalde for antibody production, Marian Bienz for the Ubx cDNA, ´ Fatima Gebauer for the pLUC-cassette vector, Iris Plischke for the injection...
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Bioavailability and toxicity of cd to microorganisms and their activities in soil
Ngày tải lên :
15/03/2014, 23:21
... whereas negative effects have been reported in forest soils (Baath, 1989) Heavy metals may reduce the substrate availability for soil respiration by forming complexes with the substrates or by killing ... with organics andyor the formation of insoluble chelates was a possible reason for its low bioavailability in clay-loam and loam soils (Lighthart et al., 1983; Dar, 1996) For the microorganisms, ... Castelporziano soil, pH 4.8, 87.5% sand, 8% silt, 4.6% clay, 2.3% TOC Forest litter MOR—brown podzolized, pH 4.5–5.2, 58% OM; Forest litter MULL—rendzina pH 3.8–4.5, 70% OM Phaeosem, pH 6.9, 2.2%...
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Báo cáo khoa học: Autophosphorylation of heme-regulated eukaryotic initiation factor 2a kinase and the role of the modification in catalysis doc
Ngày tải lên :
28/03/2014, 23:20
... nLÆmin)1 For separation, solvent A (2% acetonitrile in 0.1% formic acid) plus 5% solvent B (98% acetonitrile in 0.1% formic acid) (v ⁄ v), followed by a linear gradient up to 40% solvent B for 60 ... and C-terminal domains of HRI critical for heme sensing, and between heme and protein Therefore, multiple autophosphorylation steps are possibly important for preventing heme rebinding, inhibiting ... His120 may partially compensate for Cys409 in the dephosphorylated C409S HRI In addition, His119 or His120 alone is possibly sufficient, in the dephosphorylated form, for heme binding and modulation...
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Báo cáo khoa học: Protein folding and disulfide bond formation in the eukaryotic cell pptx
Ngày tải lên :
29/03/2014, 22:21
... cortisol generation in adipogenesis [21] L Ruddock (Oulu, Finland) reinforced the point that alternative pathways for disulfide bond formation in the ER should not be ignored: glutathione, vitamin K ... whether significant Protein folding and disulfide formation peroxide generation occurs during disulfide bond formation, an issue that has implications for cellular stress and hypoxia [22] The ER may ... meeting concluded with real enthusiasm for the challenges ahead, and a sense of pride that the field was moving forwards so rapidly, with plenty of room for cooperation, collaboration and open...
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beneficial indigenous microorganisms and bionutrients in natural farming
Ngày tải lên :
12/06/2014, 11:18
... leaves to fortify phyllosphere microbes, to soil and compost Of course, it will help improve digestion and nutrient assimilation for animals and other applications mentioned before For any kind ... population A simple general formula or recipe in fermentation can be done for plants For example, seaweeds If you simply infuse seaweeds (which are quite difficult to breakdown, therefore hard to extract ... or substituting similar materials above A basic formulation that I use is very similar to the general formulation I use for animal feeds Basic formulation consists of 80% carbohydrate, 17% protein...
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Báo cáo hóa học: " Nanofluid bioconvection in water-based suspensions containing nanoparticles and oxytactic microorganisms: oscillatory instability" potx
Ngày tải lên :
21/06/2014, 06:20
... on the concentration of microorganisms For Rb = the critical wavenumber is smaller for the rigid-free boundaries but for Rb = 120000 it is larger than for the rigid-rigid boundaries F3 294 ... equation is then solved for Ra; the critical value of Ra is again obtained by calculating the minimum value that the expression for Ra takes with respect to m Figure 1a shows that for Rb = the curve ... Figure 2c is negative for the whole range of Rn (-1.2 ≤ Rn ≤ 1.2) used for computing this figure This means that ω is imaginary and oscillatory instability does not occur for the value of Rb used...
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GENETIC DIVERSITY IN MICROORGANISMS pptx
Ngày tải lên :
28/06/2014, 08:20
... or publisher No responsibility is accepted for the accuracy of information contained in the published chapters The publisher assumes no responsibility for any damage or injury to persons or property ... the effort, because of the considerable benefits that robust phylogenetic hypotheses can provide for diverse fields of biology, both basic and applied Because even distantly related life forms ... essential tools for displaying this variation efficiently Genetics, the science of heredity, deals with the factors that are responsible for the similarities and differences between life forms and...
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INTERRELATIONSHIPS BETWEEN MICROORGANISMS AND PLANTS IN SOIL doc
Ngày tải lên :
29/06/2014, 09:20
... SOIL CHEMISTRY A BASIC ELEMENTS / Edirorsi 5B G H B O L T (Editor) SOIL CHEMISTRY B PHYSICO -CHEMICAL MODELS H E D R E G N E SOIL OF ARID REGIONS H A U B E R T mil M P I N T A TRACE ELEMENTS IN ... SOIL PHYSICS iiritl C D I R K S E N 14 G S C A M P B E L L SOIL PHYSICS WITH BASIC -TRANSPORT MODELS FOR SOIL-PLANT SYSTEMS 16 G G C C L A R I D G E ( i t i d I B C A M P B E L L ANTARCTICA; SOILS ... Prague, Czechoslovakia Organized by the Czechoslovak Society for Microbiology Czechoslovak Academy of Sciences, Prague ELSEVIER - Amsterdam - Oxford - New York - Tokyo 1989 Scientific Editor PhMr Vlastimil...
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Scientific report: "Identify some microorganisms that cause food poisoning in water use in the private slaughterhouses in Hanoi, Vietnam" pot
Ngày tải lên :
06/08/2014, 18:22
... Total Coliforms Using Brilliance E coli/coliform Selective Agar (CM1046, OXOID) for the detection and enumeration of Escherichia coli and Coliforms water samples The method for counting Coliforms ... colonies were inoculated onto nutrient agar for biochemical tests (MacFaddin, 2000) 2.4 Data analyse Counts expressed as colony forming units (cfu)/ml were transformed into log10 prior to statistical ... positive for Salmonella may be one reason explaining for the contamination in carcass Hanoi, the capital of Vietnam with more than million habitants (Laodong, 2008), is facing a high demand for food...
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