ali culture of mouse tracheal epithelial cells

... development of the CII mAb-induced arthritis model (CAIA) and the K/BxN serum transfer model of arthritis, both of which not require B cells or T cells, at least for the initiation of disease ... of seven) of u-PA-/- mice developed arthritis with an average maximum clinical score of 0.6 ± 0.2, compared with 80% (eight out of 10) of C57BL/6 mice with an average maximum clinical score of ... of nine (56%) of the sham chimeras developed disease with a low severity of 1.2 ± 0.4 The u-PA-/- ® C57 chimeras developed disease similar to u-PA-/- mice (Figure 1b); 56% (seven out of 11) of...

Ngày tải lên: 12/08/2014, 11:23

... to break tolerance via activation of Toll-like receptors The therapeutic profile of CIA in the B6 mouse was similar to that of RA, with a therapeutic action of methotrexate at a dose comparable ... reason why chicken, and not mouse or bovine, CII is arthritogenic in B6 mice is presumably due to recognition by B6 T cells of a peptide of chicken CII in the context of H-2b class II molecules ... bovine, and mouse CII (Figure 4a,b) Of particular note were the strong proliferative and cytokine responses to autologous (mouse) collagen in the LNC cultures from B6 mice, providing evidence of autoimmunity...

Ngày tải lên: 09/08/2014, 10:21

... The number of cells per mm2 was calculated by counting the number of cells within an area measured by the MCID software Preparation of purified type II collagen and crude extract of proteoglycan ... matrix These included loss of toluidine blue staining from the R930 surface of the tissue and, after 14 days in culture, development of a layer of cells on the surface of the explant (Fig 2b, c) ... time in culture (Fig 3b) There were no significant differences between either of the arthritogenic mAbs, CIIF4 or other controls in the number of cells per mm2, or in the size of the cells Available...

Ngày tải lên: 09/08/2014, 06:23

... Page of 11 (page number not for citation purposes) Figure by ALP Inhibition of IL-1β-induced upregulation of E-selectin in glEND.2 cells by ALP (a) Inhibition of IL-1β-induced upregulation of E-selectin ... analysis of anti-E-selectin antibody-stained glEND.2 cells (a murine glomerular cell line) The subsequent elucidation of a suppressive effect of ALP on IL-1β-induced NF-κB activation in glEND.2 cells ... Inhibition of leukocyte attachment to IL-1β-activated glEND.2 cells by antileukoproteinase in vitro antileukoproteinase in vitro The adherence of human granulocytes to layers of glEND.2 cells was...

Ngày tải lên: 09/08/2014, 08:22

... Measurement of right ventricular hypertrophy The degree of right ventricular hypertrophy was measured by the ratio of the weight of the right heart to left hear heart After removal of atria, the ... examination For the measurement of the wall thickness of the vessels, a computer imaging analysis software (IPLab; Scanlytics Inc., Fairfax, VA) was used to capture images of individual pulmonary arteries ... changes with chronic hypoxia Page of Lawler et al reported the first TSP-1-/- mouse [1] They showed that the absence of the TSP-1 gene was associated with the development of pneumonia In our study,...

Ngày tải lên: 10/08/2014, 09:22

... onset of fibrogenesis [3,4,32], we employed indirect immunofluorescence analysis with an anti-MOMA-2 antibody (a marker of macrophages) to examine the effect of loss of mPGES-1 on the ability of ... overexpression of profibrotic genes in established lesions [56] Investigation of the role of mPGES-1 in established fibrosis (for example, using the tight skin [Tsk] mouse [57]) is beyond the scope of the ... CD: Mechanisms of disease: the role of immune cells in the pathogenesis of systemic sclerosis Nat Clin Pract Rheumatol 2006, 2:679-685 Gabbiani G: The myofibroblast in wound healing and fibrocontractive...

Ngày tải lên: 12/08/2014, 15:22

... 13:402 http://arthritis-research.com/content/13/2/402 Page of Figure mPGES-1 genetic deletion results in reduced collagen content and myofibroblast formation in vivo (a) Hydroxyproline analysis ... Immunohistochemistry using anti-α-SMA antibody was performed mPGES-1 null mice showed a reduced number of α-SMA-expressing myofibroblasts compared with WT mice in response to bleomycin treatment (4-week treatment) ... phosphate-buffered saline (PBS)-treated mice +P < 0.05; bleomycin-treated mPGES-1 null mice compared with bleomycin-treated WT mice (d) Protein extracts from skin tissue after weeks of bleomycin or...

Ngày tải lên: 12/08/2014, 15:22

... shown) CNBr digestion of bovine CII and purification of CB10 and CB11 peptides CNBr digestion of bovine CII was performed with mg of purified bovine CII This was dissolved in ml of 70% (v/v) formic ... bovine CII were present in 23 (24%) of 94 patients with RA, (27%) of 33 with patients with PsA, (10%) of 31 patients with OA, and (1%) of 93 control sera The levels of reactivity to bovine CII compared ... CB10 were present in 84 (88%) of 96 patients with RA, (12%) of 33 patients with PsA, (6%) of 34 patients with OA, and (3%) of 93 control sera (Fig 5a) Moreover, levels of antibodies against CB10...

Ngày tải lên: 09/08/2014, 01:23

... value of 0.38 × 10-3 d.p.m recognition of the peptide in the context of both DR1 and DR4 complexes Effects of A12 and A13 on cytokine production by T cells An important characteristic of an altered ... Sweden) The concentration of CII peptide inhibiting 50% of the binding of HA peptide (IC50) was calculated from the linear portion of the curves IC50 values are the average of two determinations ... cytokine IFN-γ These experiments represent the first description of analog peptides of type II collagen recognized by T cells in the context of the human DR4 molecule that can suppress autoimmune arthritis...

Ngày tải lên: 09/08/2014, 08:22

... of adverse events of CCII was lower than that of methotrexate (MTX) [19] These results demonstrate clinical efficacy of an oral tolerance approach for RA To evaluate the efficacy and safety of ... mechanisms of oral tolerance are not fully known Animal studies have revealed that the mechanisms of induction of oral tolerance include clonal deletion, suppression of the proinflammatory Th1 cells, ... support a mechanism of oral tolerance [32] A key feature that may affect the induction of Treg cells and other suppressive mechanisms is the dose of antigen administered A low dose of antigen stimulates...

Ngày tải lên: 12/08/2014, 11:22

... capacity of DCs The effect of amiloride in vivo Our data show that pretreatment of professional APCs with amiloride prevents activation of CII-specific T cells in vitro We also confirmed the effect of ... blockade of macropinocytosis prevented intracellular accumulation of CII and resulted in profound blockade of antigen presentation to T cells The involvement of macropinocytosis in uptake of autoantigens, ... 2c,d) and suggests internalization of CII primarily by macropinocytosis Effect of uptake on activation of CII-specific T cells in vitro We studied whether prevention of CII uptake by DCs and...

Ngày tải lên: 09/08/2014, 08:22

... stimulated with 25 g/ml of lathyritic type II collagen (only cells from immunized mice) or with g/ml of ConA for 72 hours An aliquot of supernatant was taken after 24 h of stimulation in order ... 100 l aliquots of the supernatants were cultured with ì 105 cells/ well of an IL-2 dependant murine cytotoxic T cell line (CTLL), in a total volume of 200 l for 24 h and the CTLL cells were then ... Naive Dendrit ic Cells 60 50 40 5.0 2.5 0.0 Naive E Medium St imulat ion ConA F 60 50 40 Percent of Live cells % CD8+CD69+ T cells * B 3000 * 600 400 Page 11 of 14 Geometic mean of CD80 Flourescence...

Ngày tải lên: 12/08/2014, 15:22

... years thus compromising the quality of life of millions of Americans In addition, OA costs the North American economy approximately $60 billion per year Current treatment of OA includes exercise, ... ingredients Use of concomitant prohibited medication (narcotics, oral NSAIDs, topical NSAIDs) within four weeks of randomization Use of acetaminophen or ibuprofen within days of randomization ... common form of arthritis, and it is often associated with significant disability and an impaired quality of life Clinical and radiographic surveys have found that the prevalence of OA increases...

Ngày tải lên: 26/10/2012, 09:48

... RPMI-1640 medium to a concentration of × 105 cells/ ml Cells were than plated in 96-well microtitre plates at a concentration of × 105 cells/ well concentration and cultured with 40 µg/well CII in ... presence of CII by the counts/min in the presence of ovalbumin Analyses of cytokine production by ELISA Mononuclear cells were isolated from the Peyer’s patch and spleen, and cultured at a density of ... Table Proportion of IL-10-producing CD4+CD25+ T cells in lymph nodes and spleen % CD4+CD25+ IL-10-positive cells Site Induction of IL-10-producing CD4+CD25+ T cells in the spleen of tolerized mice...

Ngày tải lên: 09/08/2014, 01:23

... transcriptional activity of cells between groups R158 Arthritis Research & Therapy Vol No Lorenz et al Figure ogy to human YKL39 Interestingly, in spite of eightfold sequence coverage of the mouse genome, ... molecule col II and of YKL40 in knee cartilage, which is independent of the time lapse since surgery, of the particular joint region studied and of the structural appearance of the extracellular ... indicative of an early phase of OA development characterized by upregulation of col II, col I and YKL40, which is followed by a second phase of OA progression characterized by further upregulation of...

Ngày tải lên: 09/08/2014, 06:22

... immunizing a B10.Q mouse with 100 µg of rat CII emulsified in CFA The mouse was first boosted subcutaneously at day 35 with 50 µg of rat CII in IFA Five days before the fusion (day 210), the mouse was ... addition, this response often sharply shifted during the course of the disease, possibly reflecting a clonal origin of this epitope spreading of the antibody response, see for example mouse (Fig 1b) To ... specificity in mouse during the course of the disease shows the immunodominance of antibodies against the epitopes U1, C1 and J1 Arthritis disease course in mouse indicates the score of the individual...

Ngày tải lên: 09/08/2014, 07:20

... days of culture with antigen, cells were collected and stained with anti-CD4 PE CFSElowCD4+ cells or CFSEhighCD4+ cells were then sorted using a FACSCalibur (BD Biosciences) CFSElowCD4+ cells ... diagnosis of limited or diffuse SSc according to the criteria of the American College of Rheumatology (ACR) (1980) [18] were recruited from the Rheumatology Clinics of the University of Tennessee ... µg/ml) and anti-CD28 (1 µg/ml) Cells were incubated at a concentration of × 106 cells per ml in a 48-well plate, and supernatants were harvested after 48 hours of culture T-cell line supernatants...

Ngày tải lên: 09/08/2014, 08:22

... macrophages, T cells, and natural killer cells, and attracts leukocytes and other mediators to sites of inflammation [6-8] Cultured renal parenchymal cells, including MCs and renal tubular epithelial cells, ... terms of intrinsic factors in the kidney Materials and methods Primary culture of mesangial cells Both female NZB/W and DBA/W F1 mice were obtained from the Animal Center of the College of Medicine ... weeks of age, we chose to isolate glomeruli of female mice 7–8 weeks old for the following primary MC culture Agematched female DBA/W mice were used as the controls The preparation of primary cultures...

Ngày tải lên: 09/08/2014, 10:20

... OD280 of IgG mAb at mg/ml of 1.42 Induction of arthritis The 3-or 4-clone cocktail was prepared by mixing an equal volume of 10 mg/mL, and mice were given 0.6 or 0.8 mL of the cocktail (6 or mg /mouse) ... between the development of arthritis and the ability of mAbs to activate the complement and bind mouse CII, we measured cross-reactions with mouse CII and activation of the complement in vitro ... followed by an i.p injection of LPS (50 μg /mouse) on day The mice were observed daily after the injection of mAbs for the development of arthritis until day 10 The severity of arthritis was scored...

Ngày tải lên: 11/08/2014, 03:20

... OD280 of IgG mAb at mg/ml of 1.42 Induction of arthritis The 3-or 4-clone cocktail was prepared by mixing an equal volume of 10 mg/mL, and mice were given 0.6 or 0.8 mL of the cocktail (6 or mg /mouse) ... between the development of arthritis and the ability of mAbs to activate the complement and bind mouse CII, we measured cross-reactions with mouse CII and activation of the complement in vitro ... followed by an i.p injection of LPS (50 μg /mouse) on day The mice were observed daily after the injection of mAbs for the development of arthritis until day 10 The severity of arthritis was scored...

Ngày tải lên: 11/08/2014, 06:23