... retroaldolization/aldolization would both afford the same reaction product MATERIALS AND METHODS General methods Unlabelled DXP was prepared either enzymatically [32], or chemically (Hoeffler et al., unpublished ... yield Addition of methyl lithium gave a lactol, which opened under standard phosphorylation condition to the enantiomerically pure ketone 17, but with low yield [37] Deprotection ofthe silyl ether ... silyl ether, gave diol 22 in excellent yield Selective phosphorylation ofthe primary alcohol of 22 with dibenzylphosphate chloride [37] at low temperature followed by oxidative cleavage [38] of...
... MALDI-TOF MS The 30 kDa protein observed in the SDS ⁄ PAGE gel was in-gel digested with trypsin, and the peptides were extracted essentially according to the method of Rosenfeld et al [22] The ... and the masses ofthe peptides were identified using MALDI-TOF MS The genome sequence of P stipitis is available, and this enabled the identification ofthe corresponding ORF, which was then called ... previously The identification of a gene coding for the L- rhamnose dehydrogenase is the subject ofthe present article l- lactaldehyde is oxidized to l- lactate in an NAD-coupled reaction, as in the pathway...
... about literature review, chapter the methodology ofthe study, and chapter the results and discussion The last one is the conclusion ofthe study consisting of overview ofthe study, limitations of ... place of articulation, manner of articulation, and degree of noise in the following table below Table 1.2 English fricatives Degree of noise Place of articulation Labiodentals Dental voiceless ... Also asthma, etc thence, thither Exceptions Final *Verbs ending in a dental fricative usually have /ð/, and are frequently spelled the : bathe, breathe, clothe, loathe, scathe, scythe, seethe,...
... cells but did not lyse them (the attenuance ofthe cell mixture did not decrease) At the same time, endolysin with polymyxin B lysed the cells completely The nontreated and endolysin-treated ... respectively Table Content of Glu relative to Ala in the samples of enzymatically hydrolyzed peptidoglycan of Ps putida The contents of individual amino acids were determined after acidic hydrolysis of ... subtilis cells were lysed relatively well; however, the rate of their lysis was about 104-fold lower than that of Gram-negative cells In Listeria cells, the rate of lysis was an order of magnitude...
... And down across New Orleans and then looking down to the south and there's the whole peninsula of Florida laid out And all the hundreds of hours you spent flying across that route, down in the ... through well-intentioned programs such as building new houses for disadvantaged urban dwellers, will generally improve matters only in the short run and often lead to still more problems in the long ... control ofthe oil in their lands, Shell's position was enhanced It enjoyed an edge that has enabled it to come close to [its founder] Deterding's goal: eclipse Exxon as the world's largest oil...
... moral degeneration 3.2.1 The luxurious life ofthe upper and the middles class Leaving the world of “Oliver Twist”- the grim world ofthe poor, which was full ofthe devastating scenes and was ... skillfully used these typical images as the symbols of all the poignant cries ofthe starved and unloved in the brutal world Unable to bear such an awful living conditions, many children died in ... in the dark corner ofthe workhouses Other escaped to London and established a criminal underclass These children much rather stole and were punished than slaved in the bad conditions ofthe workhouses...
... while PDGF-C and -D mRNA levels changed dramatically [84] Concomitantly, the level of phosphorylated PDGFR-a was markedly upregulated in bleomycin-treated lungs PDGF-C mRNA could not be detected ... expression allowing binding of some PDGFs but not others Additionally, some cells display only one ofthe PDGFR isoforms while other cells express both isoforms, simultaneously or separately Third, different ... parietal glomerular cells but not in the glomerular tuft In the tubulointerstitium, PDGF-C is located in collecting ducts and the loop of Henle [81] PDGF-C protein localization in adult rodent kidneys...
... the liganded enzyme c The value of Tyr27 is excluded from the calculation ofthe average and SD d Tyr37 is disordered in the nonliganded structure of Bacillus glutaminase the displacement of all ... residues in Mglu is much smaller than those in Bacillus glutaminase In addition to the conserved amino acid residues, the displacement ofthe overall amino acid residues in Mglu is also small compared ... Disordered amino acid residues rmsd from ideality ˚ Bond length (A) Bond angle (°) ˚ Average B value (A2) Ramachandran analysis Favoured (%) Allowed (%) Generally allowed (%) Disallowed (%) ˚...
... beautiful form, perfectly developed, and the arms and hands are as delicately modelled as a woman's The face is oval, with regular features of classic mould, a short parted beard, and long hair falling ... Correggio's themes were religious The subject most often called for was that ofthe Madonna and Child Madonna is the word, meaning literally My Lady, used by the Italians when speaking of Mary, the mother ... is the source of illumination Our picture was originally called by the simple title ofThe Nativity Then the Italians, struck by the power with which the effect of midnight was produced, called...
... unseal his lips Here, too, we have the full knowledge of what consciousness is, ofthe impossibility of hiding the smallest trace of evil in the inner world; and also the dazzling brilliancy of ... help their fellows the world over SECTION IV THE APOLLONIUS OF EARLY OPINION Apollonius of Tyana[2] was the most famous philosopher ofthe Graeco-Roman world ofthe first century, and devoted the ... inspired by the circle of ideas which led to the erection ofthe innumerable d[ =a]gobas and st[=u]pas in Buddhist lands, originally over the relics ofthe Buddha, and the subsequent preservation of...
... tuberculosis L- gulono-1,4-lactone dehydrogenase B A Wolucka and D Communi oxidation of l- gulono-1,4-lactone to L- AA in animals is catalyzed by an oxygen-dependent enzyme, l- gulono-1,4-lactone oxidase ... studied 4440 further Nowadays, the l- gulono-1,4-lactone oxidase enzymes are considered exclusively as oxidases, the reaction products of which are, paradoxically, L- AA and hydrogen peroxide [8] ... (B A Wolucka, unpublished results) and could supply l- gulono-1,4-lactone for the dehydrogenase reaction In the animal pathway for vitamin C, free d- glucuronic acid is derived from UDP -d- glucuronate...
... and blocked as for ELISA, were incubated sequentially for h at 37 °C with 50 lL of biotinylated lectin (1 lgÆmL)1), followed by 50 lL of avidinylated horseradish peroxidase (1 lgÆmL)1), bound ... a-(1,3)-fucosylated, and a small portion also carries core xylosylation and ⁄ or additional GlcNAc This pattern of heterogeneity was demonstrated by MS analysis of both the glycopeptides and the released ... oligosaccharide in BG60 has been suggested to be the result of degradative reactions, rather than imperfect biosynthesis [13] Ofthe plant allergens listed in theof cial allergen database ofthe IUIS,...
... been used here to help reveal the relationship of ML2p and ML3p to MLII and MLIII The ML1p, ML2p, ML3p and ML3.1p A-chains were expressed in E coli in soluble and biologically active form The recombinant ... following cycle for 26 cycles The amplified fragments (cDNA or genomic) were purified from agarose gel and labelled with [33P]ATP[cP] using T4 polynucleotide kinase The labelled DNA was purified ... restriction endonucleases The labelled digests were separated on 2% agarose gels and the bands corresponding to the three variants of mlp genes were excised The slices were dissolved in 0.5 mL M HCl, scintillation...
... the numbers at the left are the pI values of standard proteins The arrow at the right represents the pI value of 6.84 calculated for the unmodified CLAP protein based on its deduced amino acid ... generated by Lys-C treatment of CLAP More recently, we obtained the sequence EAKNLVDLAESLGLSILVKALE from Edman degradation of an untreated preparation of CLAP indicating that the N-terminus ofthe ... polypeptide 44–306, of clone of CLAP using the CLUSTALW multiple alignment program The determined secondary structure (alpha helices and beta strands), as based on the spatial structure ofthe Drosophila...
... Ta, l and lD, cells transfected with pcDNA3 ⁄ mSMOa, l and lD, ⁄ V5-TAG plasmids; C, no-template control generated by the PCR was verified by sequence analysis The deletion mutant ofthe mSMOl protein ... transiently transfected neuroblastoma N18TG2 cells Transiently transfected cells are indicated on the left side ofthe figure Anti-V5 and propidium iodide (PI) dye columns indicate the secondary immunofluorescence ... and pmSMOl plasmids, as described previously [10], and with the pmSMOlD plasmid, were cultured at 30 °C in Luria–Bertani (LB) medium, containing 50 lgÆmL)1 ampicillin, to an attenuance (D) of...
... panels; panels j–r, nuclear DNA stained with DAPI ofthe cells in the same views as in panels a–i, respectively (D) Subcellular localizations of C-terminal deletion mutants described on the top of ... panel were studied as described in (C) Panels a d, fluorescent signals of cells; panels e–h, nuclear DNA stained with DAPI ofthe cells in the same view as in panels a d, respectively Plus and minus ... on the top of each panel; panels f–j, nuclear DNA stained with DAPI ofthe cells in the same views as in panels a–e located in the nucleus (panel e) Immunoblotting of cell lysates confirmed the...