ae1 ae3 pan cytokeratin monoclonal antibody

... of a non-neutralizing 3E1 mAb, raised against cathepsin B (C) Left panels are differential interface contrast images; right panels are images of green fluorescence after hydrolysis of DQ-collagen ... whereas the level of the complex formed between cathepsin B and the antibody or its Fab increased This suggests that the binding of the antibody can displace the occluding loop from its endopeptidase ... were detected using 3,3¢,5,5¢-tetramethylbenzidine (Sigma) and H2O2 as substrate The monoclonality of the antibody was assessed by IEF using the PhastSystem (Pharmacia) Kd between 2A2 mAb and...

Ngày tải lên: 18/02/2014, 11:20

... Ó FEBS 2003 A PAI-1-protecting monoclonal antibody (Eur J Biochem 270) 1673 Fig Localization of the epitope for Mab-1 in the three-dimensional ... 0.01) and Q58/74AD307/318A (15 ± 3%; n ¼ 3; P < 0.01) Monoclonal antibodies Mab-1 was produced and purified as described previously [29] Two other monoclonal antibodies against PAI-1, Mab2 [29,36,37] ... microtiter wells, using an antibody concentration of 2.5 lgÆmL)1 and a buffer of 50 mM NaHCO3, pH 9.6 After blocking with milk, dilution series of recombinant PAI-1, spanning a concentration range...

Ngày tải lên: 20/02/2014, 11:20

... Ó FEBS 2002 short sugar side chains (e.g T, ST and TF antigens) [8,9] The monoclonal antibody SM3 was raised in mice against partially deglycosylated human MUC-1 It shows a high ... to the antibody [18] Conventional pepscan analysis however, does not allow easy analysis of the contribution of the carbohydrate portion To assess the involvement of carbohydrates in antibody ... MUC-1 peptide PDTRP and the MUC-1 glycopeptide PDT(O-aD-GalNAc)RP (cf Figure 1) bound to the monoclonal antibody SM3 MATERIALS AND METHODS Chemicals Chemicals for peptide synthesis were obtained...

Ngày tải lên: 21/02/2014, 15:20

... washing, the wells were incubated with lg of a rabbit polyclonal antibody against Fn Bound antibody was detected by incubation with secondary antibody [HRP-conjugated goat anti-(rabbit IgG)] (B, D) Western ... incubation with rabbit polyclonal antibody against Fn followed by addition of peroxidase-conjugated goat anti-(rabbit IgG) Monoclonal antibodies against FnBRs of FnBPB A panel of mouse mAbs was produced ... rabbit polyclonal antibody against Fn Binding of the polyclonal antibody (A) or mAb (B) was detected by incubating the wells with HRP-conjugated goat anti-(rabbit IgG) or secondary antibody [HRP-conjugated...

Ngày tải lên: 06/03/2014, 22:21

... the Pk antigen as determined by a monoclonal antibody J Immunogenet 12, 213–220 32 Oosterwijk, E., Kalisiak, A., Wakka, J., Scheinberg, D & Old, L.J (1991) Monoclonal antibodies against Gala1–4Galß1–4Glc ... Fellows, M & Tursz, T (1983) A glycolipid antigen associated with Burkitt lymphoma defined by a monoclonal antibody Science 220, 509 Li, S.-C., Kundu, S.K., Degasperi, R & Li, Y.-T (1986) Accumulation ... a2-interferon receptor [27] Verotoxin B subunit and monoclonal anti-Gb3 can induce apoptosis [28,29], particularly in lymphoid cells [30] Tissue screening with monoclonal anti-Gb3 indicates a wider Gb3...

Ngày tải lên: 16/03/2014, 16:20

... equilibrium-binding constants of M27 for the different forms The antibody- binding site shown in Fig is sufficiently ˚ close to the RCL of AT (40–50 A) to allow antibodymediated blocking of the formation of the ... complete and was not influenced by a 10-fold increase in antibody concentration (data not presented) This suggests that the binding sites on AT for antibody and heparin are independent of one another ... 271, 6523– 6529 40 Asakura, S., Matsuda, M., Yoshida, N., Terukina, S & Kihara, H (1989) A monoclonal antibody that triggers deacylation of an intermediate thrombin–antithrombin III complex J...

Ngày tải lên: 17/03/2014, 10:20

... Albanese J, Beenhouwer DO, Cardarelli PM, Cuison S, Deng DF, Deshpande S, Diamond JH, Green L et al (2005) Production of human monoclonal antibody in eggs of chimeric chickens Nat Biotechnol 23, 1159–1169 ... Institute of Agrobiological Science (Tsukuba, Japan) The larvae were reared at 25 °C on an artificial diet (Silk Mate PM, Nosan Corp, Kanagawa, Japan) Construction of the vectors used to generate ... Asahipak NH2P-50 column (0.2 · 15 cm; Showa Denko, Tokyo, Japan) and Cosmosil 5C18-P column (0.2 · 25 cm; Nacalai Tesque, Kyoto, Japan), respectively, as described previously [57] The fractionated...

Ngày tải lên: 23/03/2014, 04:20

... Kanto Chemical, (Tokyo, Japan) Bio-Gel P-2 and P-6 resins were purchased from Bio-Rad Laboratories (Richmond, CA, USA) Dowex-50 resin was purchased from Dow Chemical Company (Midland, MI, USA) ... Jpn 35, 1409–1425 12 Ishihara K, Kurihara M, Eto H, Kasai K, Shimauchi S & Hotta K (1993) A monoclonal antibody against carbohydrate moiety of rat gastric surface epithelial cellderived mucin Hybridoma ... derived from mammalian gastric gland mucous cells: epitope recognized by newly characterized monoclonal antibody Biochem J 318, 409–416 14 Ota H, Hayama M, Nakayama J, Hidaka H, Honda T, Ishii K,...

Ngày tải lên: 23/03/2014, 09:21

... Although monoclonal DPP IV antibody 4D10 arrests the cell cycle FEBS Journal 276 (2009) 6548–6559 ª 2009 The Authors Journal compilation ª 2009 FEBS 6555 Epitope of monoclonal DPP IV antibody ... fragment (B) 6A3 immunoblotting (upper panel), 6A3 immunoprecipitation followed by anti-MBP immunoblotting (middle panel) and anti-MBP immunoblotting (lower panel) for 50 ng of amylose agarose bead-purified ... 276 (2009) 6548–6559 ª 2009 The Authors Journal compilation ª 2009 FEBS 6549 Epitope of monoclonal DPP IV antibody T.-T Hung et al demonstrated to be pericellular polyFN because it was removed...

Ngày tải lên: 29/03/2014, 22:21

... establishes that the epitope of the F11 antibody is in the C-lobe of hTF, specifically within residues 365–401 of the C1 domain Furthermore, binding of the F11 antibody to hTF inhibits binding to ... goal was to use an epitope mapping study with an antibody known to inhibit binding of hTF to TFR to delineate the residues that comprise the antibody binding site and thus, by extension, the ... F11 antibody is binding to surface residues, it is likely that the epitope is restricted to residues 365–385 of the hTF C-lobe The mAb F11 blocked binding of hTF to the TFR; this suggests the antibody...

Ngày tải lên: 30/03/2014, 11:20

... Introduction Generation of Hybridomas: Permanent Cell Lines Secreting Monoclonal Antibodies In Vitro Production of Monoclonal Antibody 12 Batch Tissue-Culture Methods 12 Semipermeable-Membrane-Based ... Method 24 Disadvantages of Mouse Ascites Methods 24 Large-Scale Production of Monoclonal Antibodies 25 Monoclonal Antibody Production for Diagnostic and Therapeutic Purposes 25 In Vivo and In ... replace "500 mg/ml and 300 mg/ml" with "500 µg/ml and 300 µg/ml" Monoclonal Antibody Production Committee on Methods of Producing Monoclonal Antibodies Institute for Laboratory Animal Research...

Ngày tải lên: 11/04/2014, 07:16

... antibody References Teng, N N H., Reyes, G R., Bieber, M., Fry, K E., Lam, K S., and Herbert, J M (198.5) Strategies for stable human monoclonal antibody production, in Human Hybridomas and Monoclonal ... cover Expand antibody- positive cell lines into 12- or 24-well cluster plates Repeat cloning procedure if necessary to obtain stable antibodyproducing cell lines (see Notes 5-7) 16 Stocks of antibody ... hybrids producing antibody, transfer cells from wells containing a single colony to 24-well plates to expand the culture, and then transfer to 25-cm2 tissue-culture flasks 10 Following expansion of...

Ngày tải lên: 11/04/2014, 09:52

... motavizumab We previously showed that the superior neutralizing activity of this anti-RSV monoclonal antibody compared with palivizumab was associated with further reductions in RSV replication ... treatment with either PBS or an IgG1 isotypematched control antibody, MEDI-507, at the same time of the administration of the anti-RSV antibody had no effect on the cytokine profile or other clinical ... (IFN-γ) and granulocyte macrophage colony-stimulating factor (GM-CSF) were measured using a multiplex antibody assay Except for IL-10, MCP-1, IL-1β and IL-12p70 concentrations, the effect of motavizumab...

Ngày tải lên: 18/06/2014, 18:20

... motavizumab We previously showed that the superior neutralizing activity of this anti-RSV monoclonal antibody compared with palivizumab was associated with further reductions in RSV replication ... treatment with either PBS or an IgG1 isotypematched control antibody, MEDI-507, at the same time of the administration of the anti-RSV antibody had no effect on the cytokine profile or other clinical ... (IFN-γ) and granulocyte macrophage colony-stimulating factor (GM-CSF) were measured using a multiplex antibody assay Except for IL-10, MCP-1, IL-1β and IL-12p70 concentrations, the effect of motavizumab...

Ngày tải lên: 20/06/2014, 01:20

... human monoclonal antibodies against H5N1 influenza PLoS Med 2007, 4:e178 Hanson BJ, Boon AC, Lim AP, Webb A, Ooi EE, Webby RJ: Passive immunoprophylaxis and therapy with humanized monoclonal antibody ... examine the ability of the humanized antibody VN042 to bind to the selected HAs, ELISA was performed Figure shows the level of binding detected with ug/mL VN04-2 antibody and several serial dilutions, ... the 140s loop may be the main determinants of antibody binding for VN042, but residues outside of this region may also contribute to the overall antibody binding affinity Previous studies with...

Ngày tải lên: 20/06/2014, 01:20

... was then diluted 1:3 in growth medium and incubated with the test antibody at RT for h DEAE-dextran solution was added to the VLPantibody mix to a final concentration of 10 ug/ml, before transferring ... control of pandemic influenza Proc Biol Sci 2007, 274:1675-1684 Hanson BJ, Boon AC, Lim AP, Webb A, Ooi EE, Webby RJ: Passive immunoprophylaxis and therapy with humanized monoclonal antibody specific ... conjugated) was used as the secondary signal producing antibody 1-Untreated IN597-FO; 2-Trypsin-cleaved IN597-FO The humanized protective antibody VN04-2 was used as a positive control for presence...

Ngày tải lên: 20/06/2014, 01:20

Ngày tải lên: 07/08/2014, 17:22

Ngày tải lên: 07/08/2014, 20:23

... human leukocyte antigen B27 transgenic rats treated with control assays monoclonal antibody IgG (filled bars) or monoclonal antibody C11C1 (open bars) at day 16 of the experimental protocol Values ... inflammation Effect of mAb C11C1 on HLA-B27 transgenic rats colonic inflammation (a) Effects of monoclonal antibody (mAb) C11C1 on diarrhea in human leukocyte antigen B27 (HLA-B27) rats Stool score ... inflammatory arthritis C11C1 on HLA-B27 transgenic rat inflammatory arthritis (a) Effects of monoclonal antibody (mAb) C11C1 on clinical signs of arthritis in human leukocyte antigen B27 (HLA-B27)...

Ngày tải lên: 09/08/2014, 06:22

... influence of dose Therefore, these data overall raise concerns about the safety of anti-TNF monoclonal antibody therapy in RA, especially when used at high doses Commentary However, there are a ... the BSR References Bongartz T, Sutton AJ, Sweeting MJ, Buchan I, Matteson EL, Montori V: Anti-TNF antibody therapy in rheumatoid arthritis and the risk of serious infections and malignancies: systematic...

Ngày tải lên: 09/08/2014, 08:22