adsorbed ecm proteins on biomaterials facilitate cell attachment

Báo cáo khoa học: The inﬂuence of cold shock proteins on transcription and translation studied in cell-free model systems pdf

... interaction in the combined transcription translation assay At a TmCSP concentration of 139.5 lm (the TmCSP concentration for 50% inhibition of the protein expression), the protein expression was ... shock proteins on the combined cell- free transcription/translation of proteins RNA secondary structures, especially hairpin loops, are supposed to be the main reason why many genes can only be ... our E coli cell- free system to investigate the effects of the putative RNA chaperones on the protein expression CSPs in different concentrations were used to monitor the concentration dependent...

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Effect of cyclooxygenase inhibition on cholesterol efflux proteins and atheromatous foam cell transformation in THP-1 human macrophages: a possible mechanism for increased cardiovascular risk pot

Danh mục: Báo cáo khoa học

... following conditions: (a) RPMI control, (b) RPMI containing NS398 (10 to 100 μM), (c) RPMI containing indomethacin (0.5, 5, and 50 μM), (d) RPMI containing SC560 (0.001 to 0.1 μM), and (e) RPMI containing ... foam cell transformation Selective inhibition of COX-1 with SC560 (0.001 μM) also increased foam cell transformation (87% ± 10% above control, n = 3, p < 0.001) PGD2 did not inhibit foam cell ... important contributor to atheromatous plaque progression under conditions of COX-2 inhibition (Figure 9) Although the selective COX-2 inhibitor NS398 is not used in humans, the concentration achieved...

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Identifying differentially expressed cell membrane proteins on fetal primitive erythroblasts and adult anucleate erythrocytes for non invasive prenatal diagnosis

Danh mục: Tổng hợp

... uncharacterised location within FNRBCs 143 5.4 Comparison of FNRBC and AARBC membrane proteins 146 5.5 Conclusions 150 Additional notes on Erythrocyte membrane band 152 CHAPTER GENERAL DISCUSSION 155 6.1 ... Categorization of proteins identified from AARBC membrane preparations 145 Figure 5.10 Categorization of proteins identified from FNRBC membrane preparations 145 Figure 5.11 Comparison of subcellular ... Tanner MJA & Anstee DJ (1993) Red Cell Membrane and Red Cell Antigens London: Bailliere Tindall Publications.) Peripheral proteins Peripheral proteins are located on the cytoplasmic surface of the...

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Báo cáo khoa học: Effects of a novel arginine methyltransferase inhibitor on T-helper cell cytokine production pot

Danh mục: Báo cáo khoa học

... activated T cells (NFAT)driven promoter activity and IL-2 secretion [6] Additionally, arginine methylation regulates cytokine gene transcription in T helper (Th) cells through arginine methylation of ... investigations focused on compound FEBS Journal 277 (2010) 2096–2108 ª 2010 The Authors Journal compilation ª 2010 FEBS 2097 Modulation of T-helper cell function by AMI-1 O2C NH3 NH2 NH2 N Me K Bonham ... isotype control from 293T -cell extracts was subjected to in vitro methylation reactions using the indicated concentrations of AMI-1 or compound and MBP as substrate (left panel) Reaction inputs...

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Báo cáo khoa học: Protein–protein interactions and selection: generation of molecule-binding proteins on the basis of tertiary structural information potx

Danh mục: Báo cáo khoa học

... constructive selection and functionalization not only of antibody fragments, but also of small, nonantibody proteins (Fig 1) Accurate structural descriptions of protein–protein interaction provide support ... binding proteins using selection methodology, and recent structural visualization of candidate proteins by X-ray or NMR structural analyses and the construction of artiﬁcial libraries allow constructive ... novel target proteins, and the ﬁrst and second minireviews focus on the detection of protein–protein interactions [8,9] In this third minireview, we focus on the molecular evolutional methodology...

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Báo cáo khoa học: Investigation of the effects of sulfonylurea exposure on pancreatic beta cell metabolism pptx

Danh mục: Báo cáo khoa học

... compared to control conditions Previous studies on tolbutamide have demonstrated that under the same conditions as described in this study, the insulin content does not change [27] Prolonged exposure ... prolonged exposure to sulfonylurea drugs on beta cell metabolism using a combination of different NMR techniques, and utilizing the well-characterized clonal glucose-responsive pancreatic beta cell ... perspectives on the actions of sulphonylureas and hyperglycaemic sulphonamides on the pancreatic betacell Diabete Metab 20, 157–162 Kamp F, Kizilbash N, Corkey BE, Berggren PO & Hamilton JA (2003) Sulfonylureas...

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Báo cáo hóa học: "Comparison of anti-CD3 and anti-CD28-coated beads with soluble anti-CD3 for expanding human T cells: Differing impact on CD8 T cell phenotype and responsiveness to restimulation" pot

Danh mục: Hóa học - Dầu khí

... Biotech Monitoring T cell division and early expansion using CFSE labeled cells To monitor cells division and expansion during the early days after stimulation, cells were CFSE-labeled and monitored ... intracellular IFNg or TNFa in response to PMA/A23187 stimulation (Figure 5) T cell response to restimulation On occasion, the number of cells generated by one cycle of T cell expansion may be insufficient ... expanding T cells, our studies demonstrate substantial differences in their impact on CD8 cells that merit consideration in Page of 15 situations where preservation of the CD8 T cell response in...

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Báo cáo sinh học: "The impact of Metastasis Suppressor-1, MTSS1, on oesophageal squamous cell carcinoma and its clinical significance" doc

Danh mục: Hóa học - Dầu khí

... the cells significantly suppressed cell migration to close the wound compared to the controls (p < 0.05) The result was also consistent with observations in MTSS1 knockdown cells Cell migration ... Our functional studies have demonstrated that the MTSS1 over-expression resulted in a dramatic reduction in tumour cell migration, invasion and growth, and an increase in cell adhesion The loss ... vitro studies Regulation of MTSS1 expression had an impact on oesophageal squamous cell carcinoma cell aggressiveness Effects of MTSS1 over-expression or knockdown on in vitro cell growth We first...

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o cáo hóa học:" The impact of Metastasis Suppressor-1, MTSS1, on oesophageal squamous cell carcinoma and its clinical significance" docx

Danh mục: Hóa học - Dầu khí

... the cells significantly suppressed cell migration to close the wound compared to the controls (p < 0.05) The result was also consistent with observations in MTSS1 knockdown cells Cell migration ... Our functional studies have demonstrated that the MTSS1 over-expression resulted in a dramatic reduction in tumour cell migration, invasion and growth, and an increase in cell adhesion The loss ... vitro studies Regulation of MTSS1 expression had an impact on oesophageal squamous cell carcinoma cell aggressiveness Effects of MTSS1 over-expression or knockdown on in vitro cell growth We first...

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Báo cáo hóa học: "Biocompatible micro-sized cell culture chamber for the detection of nanoparticle-induced IL8 promoter activity on a small cell population" pot

Danh mục: Hóa học - Dầu khí

... long-term cultivation and non-invasive quantification of specific cellular responses of adherent individual cells in a small defined cell layer cultured on miniaturized Si3 N4 membranes with cell ... from cell to cell, e g., depending on the cell cycle stage or on previous exposures Therefore, the analysis and quantification of single -cell responses will provide important information on the ... the continuous observation of GFP expression and the quantification of concentration- and time-dependent nanoparticle-induced IL8 promoter activation in adherent cells of a small cell population...

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Báo cáo sinh học: " Systematic identification of regulatory proteins critical for T -cell activation" doc

Danh mục: Báo cáo khoa học

... of infection (Additional data file 2); distinction between CD3-, CD3low and CD3high cell populations (Additional data file 3); distribution of Dox ratios among the 2,828 single -cell clones analyzed ... levels of CD69 expression and strong induction following TCR stimulation (see Additional data file with the online version of this article for details of the selection and infection procedures) The ... T -cell activation We used up-regulation of the cell- surface marker CD69 in T cells to monitor TCR activation; CD69 as an activation marker has been well validated [6], more recently using T cells...

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Báo cáo khoa học: " Role of p53 mutation in the effect of boron neutron capture therapy on oral squamous cell carcinoma" ppsx

Danh mục: Báo cáo khoa học

... suppressed more strongly than SAS/mp53 cells At a dose of Gy, the Figure BNCT Suppression of the colony formation of oral SCC cells by Suppression of the colony formation of oral SCC cells by BNCT ... Suppression of the colony formation of oral SCC cells by BNCT SAS/neo and SAS/mp53 cells were treated with BNCT, and the survival ratios were calculated based on colony formation In both cell lines, ... citation purposes) Radiation Oncology 2009, 4:63 http://www.ro-journal.com/content/4/1/63 Figure Induction of apoptotic cells with the fragmentation of nuclear DNA by BNCT Induction of apoptotic cells...

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Báo cáo y học: "The influence of serum, glucose and oxygen on intervertebral disc cell growth in vitro: implications for degenerative disc disease" potx

Danh mục: Báo cáo khoa học

... No Johnson et al Table The major phenotypes of bovine intervertebral disc cells in response to altered culture conditions Serum Monolayer With Cell proliferation ↑↑↑ Cell senescence ↔ Cell death ... monolayer Hence, whereas 24% ± 2% of cells in control monolayers were Ki-67-immunopositive, only 3% ± 0.8% of cells were positive in control alginate cultures None of the cells in serum-deprived alginate ... linked with cell senescence or cellular adaptations to extracellular stresses [26,30] As SA-β-gal staining was seen in cell populations that had undergone growth arrest, it can be concluded that...

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báo cáo khoa học: "Nanofibers and nanoparticles from the insectcapturing adhesive of the Sundew (Drosera) for cell attachment" ppsx

Danh mục: Báo cáo khoa học

... coverslips, for cell attachment studies, the coverslips were cut to mm with a diamond etched pen These smaller coverslips were then cleaned by sonication in acetone, ethanol and deionized water ... accurate Publication quality scans were conducted at a scan speed of less than ln/s and a resolution of 1024 × 1024 pixels Transmission electron microscopy Transmission electron microscopy (TEM) ... attached cells per mm2 yielded 147 cells/ mm2 for the negative control, 1681 cells/mm for the positive control, and 1253 cells/mm for the Sundew adhesive coated surface Due to the non-uniformity...

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báo cáo khoa học: " On-chip constructive cell-Network study (I): Contribution of cardiac fibroblasts to cardiomyocyte beating synchronization and community effect" pdf

Danh mục: Báo cáo khoa học

... noted that this asynchronization phenomenon was observed in all the above two types of fibroblast-cardiomyocyte synchronization For the confirmation of long term cultivation, we also have observed ... beating rhythms indicate the condition of their synchrony, i.e., green line indicates synchronized condition, whereas yellow line indicates asynchronized condition (b) Magnified graph of Figure ... electrical conductivity was maintained among CM-F-CM networks One possible reason for asynchronization occurrence might be the limited intercellular communication due to the lower electrical conductivity...

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Báo cáo sinh học: " Application of a haematopoetic progenitor cell-targeted adeno-associated viral (AAV) vector established by selection of an AAV random peptide library on a leukaemia cell line" pot

Danh mục: Báo cáo khoa học

... 2-mediated transduction in primary human bone marrow-derived CD34+ hematopoietic progenitor cells: donor variation and correlation of transgene expression with cellular differentiation J Virol 1997, ... pMT187-xx2 (containing the respective clone) using the Metafectene transfection reagent (Biontex Laboratories, Munich, Germany), according to the manufacturer's conditions After 48 h, cells were ... 5'-ACTCACCCGCCGTCTGGATCATGAC-3') Functional titration was performed as described previously [25] Transduction One day before transduction, cells were seeded into 24 well plates at a density of 2.5 or × 104 cells/well in...

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Roles of tuberous sclerosis complex proteins in autophagy and cell death

Danh mục: Kỹ thuật - Công nghệ

... Generation of destination vector pLenti4/TO/V5-DEST-TSC2 69 2.13.2.1 Identification and preservation of positive clone 69 2.13.2.2 LR Recombination Reaction 69 vi 2.13.3 Construction of ... 3.2.5 Nutrients supplementation protects against cell death in TSC2+/+cells, but enhances cell death in TSC2-/- cells under starvation condition 92 3.3 Discussion 95 CHAPTER TSC ... degradation of glycogen will require more investigations 1.1.3.2 Autophagy in cellular degradation Autophagy is a constitutively active process that functions at low levels under cell basal conditions...

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STRUCTURES OF ADSORBED HALIDE ANIONS ON a COPPER ELECTRODE STUDIED BY IN SITU SCANNING TUNNELING MICROSCOPY

Danh mục: Vật lý

... adsorption of iodide anions on the same substrate surface the supporting electrolyte (10 mM HCl) was replaced by STRUCTURES OF ADSORBED HALIDE ANIONS ON A COPPER ELECTRODE STUDIED BY 241 a solution ... into contact with the iodide anions containing solution (red curve) The most striking deviation from the pure supporting electrolyte CV, in fact, is seen in the anodic potential regime As a consequence, ... the current conditions, and, therefore, should be electroreduced to iodide anions according to [24]: I2 + 2e− −→ 2I − (electroreduction) (4) The same results and reasonable explanations have been...

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The influence of the xin repeat containing proteins on the development of pressure induced cardiac hypertrophy in mice

Danh mục: Tổng hợp

... Propagation of APs follows the specialized conduction system of the heart as these cells allow the highest APconduction velocity (Boron, 2009) Function of the AP is to initiate the contraction of ... shows the cellular events leading to contraction and relaxation in a cardiac contractile cell Introduction 18 The Ca2+ ions bind to troponin and initiate the cycle of cross-bridge formation and ... determine the consequences of a disruption of both XIRP genes 1.1.5 The excitation-contraction coupling of cardiomyocytes Contraction of cardiac muscle cells is not initiated by neurons as in skeletal...

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A CFD analysis on the effect of ambient conditions on the hygro-thermal stresses distribution in a planar ambient airbreathing PEM fuel cell

Danh mục: Môi trường

... Navier-Stokes equations, i.e the continuity equation, the mass conservation equation for each phase yields the volume fraction (r ) and along with the momentum equations the pressure distribution inside ... diffusion and convection Multiple species are considered in the gas phase only, and the species conservation equation in multicomponent, multi-phase flow can be written in the following expression ... (b) longitudinal cross section of 3 -cell fuel cell stack ISSN 2076-2895 (Print), ISSN 2076-2909 (Online) ©2011 International Energy & Environment Foundation All rights reserved International...

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