additional resources and advanced material

... (a helix signature), 218 nm (b strand signature), 202–204 nm (random coil signature) In WhiB5 and WhiB6, the structure was dominated by a helices and b strands and the proportion of these structural ... refolding were  98% pure and were brownish (Figs S1 and S2) The presence of four conserved cysteines and the brownish appearance of purified WhiB1, WhiB2, WhiB5, WhiB6 and WhiB7 indicated the presence ... WhiB6 and WhiB7 the loss was  8–10% After 48 h of air exposure, losses were as follows:  80% in WhiB1,  75% in WhiB2 and WhiB5,  65% in WhiB3,  60% in WhiB4, and  35–40% in WhiB6 and WhiB7...

Ngày tải lên: 18/02/2014, 12:20

... upland regions of the British Isles in the Younger Dryas Compilations of processes, mechanisms and chronologies can be found in Ballantyne and Harris (1994) and Gordon and Sutherland (1993) and ... applied and environmental sciences and contributes to face and solve several environmental and geological issues In this fields geomorphological analysis provides methods and tools for mapping of landforms ... hazards and resources and for mapping of other phenomena through their association with landform It is possible to identify rates of change of hazardous phenomena and causes of changes and hazards,...

Ngày tải lên: 28/06/2014, 15:20

... these molecules and molecular descriptors for lipophilicity (C log P) and molar bulk (CMR) are shown in Fig Their hydrolytic and photochemical stability, and their solubility and phase distribution ... (antioxidant activity) Materials and methods 2.1 Materials Five different symmetrical and one asymmetric curcuminoids were synthesised using the methods of Pabon (1964) and Masuda et al (2001), ... C-l and C-2 the concentrations at most pH values (i.e., pH < 9.5 and 9.0, respectively) were below the detection limit of the HPLC system Compounds C-3 and C-6 were only investigated at pH and...

Ngày tải lên: 01/07/2014, 13:23

... energy range MATERIALS AND METHOD 2.1 Copolymer Samples The hydrophilic copolymer materials used in this study are made from a combination of vinyl pyrolidone (VP, a hydrophilic monomer) and methyl ... as ED1S and ED4C The MMA to VP monomers composition ratio for ED1S is 1:3 and for ED4C is 1:4 The major elemental composition of the hydrophilic material is comparable to that of tissue and other ... The percentage elemental composition of ED1S and ED4C as compared to that of some tissues and other tissue-equivalent materials (ICRU 1989).10 ED1S and ED4C contain the major elements of real...

Ngày tải lên: 07/08/2014, 14:20

... 270 and 320 bp for and 10 isolates, respectively On the other hand, different sized amplicons of 100, 150, 200, 230, 240, 250, 270, 290 and 340 bp were observed for 8, 1, 1, 1, 2, 1, 1, and S ... seg and sei, sed and sej, sec and tst of S aureus, observed in the present study had also been described by Zhang et al [36], Jarraud et al [15], Stephan et al [30] and Akineden et al [1], and ... domain (cnaA) and B domain (cnaB) [32], capsular polysaccharide (cap5) and (cap8) [23], and staphylococcal exotoxin like protein (set1) [35] The sequences of the oligonucleotide primers and the temperature...

Ngày tải lên: 07/08/2014, 17:22

Ngày tải lên: 07/08/2014, 18:21

... mainly protein kinases and their associated factors, including Cdc7p and the small GTPase Spg1p, Sid1p and the phosphatase Cdc14p, and Sid2p and its subunit Mob1p (Fankhauser and Simanis, 1994; Guertin ... (Dettmer and Friml, 2011; Macara and Mili, 2008; Royer and Lu, 2011; Sabherwal and Papalopulu, 2012) The specification of cell tips in S pombe is regulated by multipile molecular pathways and complexes ... of S-phase and mitosis, as well as Wee1p that negatively regulates mitotic transition (Fantes and Nurse, 1977; Fantes and Nurse, 1978; Nurse, 1975; Nurse and Bissett, 1981; Nurse and Thuriaux,...

Ngày tải lên: 09/09/2015, 11:21

... 2.3 A motion graph built from two initial clips A and B Clip A is cut into A1 and A2, and clip B is cut into B1 and B2 Transition clips T1 and T2 are inserted to connect the segments Similar ... Figure 2.3 A motion graph built from two initial clips A and B Clip A is cut into A1 and A2, and clip B is cut into B1 and B2 Transition clips T1 and T2 are inserted to connect the segments Similar ... PhysX and Bullet, and the users can specify the maximum number of iterations allowed Vortex provides both a standard and an iterative LCP solver, just like ODE However, we only test its standard...

Ngày tải lên: 02/10/2015, 17:15

... quality and low cost, to manage plant dynamics altered by material recycle and energy integration, to satisfy environmental and safety regulations, and to have a certain degree of flexibility to handle ... reaction (Freedman et al., 1986; Nourreddini and Zhu, 1997; Kusdiana and Saka, 2001; Vicente et al., 2005; Singh and Fernando, 2007; Jain and Sharma, 2010) and development of heterogeneous catalysts ... knowledge and expertise and their cheerful company, Mr Boey for always being approachable and helpful, Ms Samantha Fam and Mr Mao Ning for providing support for computer equipments and software...

Ngày tải lên: 13/10/2015, 15:57

... indicator and the basis for calculations of heating and cooling energy demand [1] or for estimating bin data and heating and cooling degree-days at any base [2, 10, 11] Table shows the monthly and ... cooling demands is 28.5% and for Thessaloniki 13.2% Obviously, directly proportional to the energy demand for heating and cooling is the fuel (diesel oil) and electricity consumption and hence ... measures applied to dwellings Energy and Buildings 200, 37, 982-95 Frank Th Climate change impacts on building heating and cooling energy demand in Switzerland Energy and Buildings 2005, 37, 1175-85...

Ngày tải lên: 05/09/2013, 16:10

... mg/L Table Groundwater assets in 1998 Province 8S Gippsland 8S Gippsland 8S Gippsland 8S Gippsland 8S Gippsland 8S Gippsland 8S Gippsland Sustainable yield by salinity category(a) Fresh Marginal ... equipment; and other machinery and equipment Wood and paper product manufacturing (includes only log sawmilling and timber dressing; and paper and paper product manufacturing) Petroleum and chemical ... Clothing and footwear Wood and wood products Paper, printing and publishing Petroleum and coal products Chemicals Rubber and plastic products Non-metallic mineral products Basic metals and products...

Ngày tải lên: 17/02/2014, 10:20

... between R81 and P82 and between G90 and I91 A similar cis peptide between G93 and V94 is found in Tm SurE also Overall structural features of the protein St SurE is an aba sandwich protein and adopts ... archaic and thermophilic homologues The St SurE monomer consists of 13 b-strands, six a-helices and three 310-helices The core of the protein is made up of a nine-stranded b-sheet flanked by a1, a5 and ... are represented by pink, cyan, blue and yellow, respectively The tetrameric interactions are between the subunits A and C, and B and D, by way of the b hairpins and the loop, which connects b2 to...

Ngày tải lên: 18/02/2014, 14:20

... group and contained one monomer in the asymmetric unit Cell dimensions and details of data collection are shown in Table Expression and purification of bsSHMT and E53QbsSHMT The expression and purification ... supported by the Department of Science and Technology and DBT We thank Babu and James for their help during data collection VR and BSB thank the Council for Scientific and Industrial Research, Government ... Gly and Ser external aldimine complexes of E75Q and E75LrcSHMT, the electron density for the ligands was not well defined [15] In contrast, excellent electron density was observed for the ligands...

Ngày tải lên: 18/02/2014, 16:20

... equimolar amounts of Akazara scallop TnC Lanes h and m, ATnI-52K; lanes i and n, ATnI-19K; lanes j and o, ATnI1)128; lanes k and p, ATnI130)252; lanes l and q, ATnI232)292; lane r, Akazara scallop ... pellets (P) and supernatants (S) were redissolved in equivalent volumes of M urea solution and then run on SDS ⁄ PAGE Lanes a and d, in the absence of both TnC and Ca2+; lanes b and e, in the ... Ojima and Nishita [21,34] Rabbit fast skeletal myosin and F-actin were prepared by the method of Perry [35] and Spudich and Watt [36], respectively All measures were taken to minimize pain and...

Ngày tải lên: 20/02/2014, 01:20

... ðRi À RavÞ2 Á ðGi À GavÞ2 i ð4Þ i where Ri and Gi are the red and green intensities of voxel I, respectively, and Rav and Gav the average values of Ri and Gi, respectively It is used for describing ... Dopamine D1 and D2 receptors dimerization S Łukasiewicz et al A B C D Fig Fluorescence emission spectra of HEK293 cells expressing the CFP- and YFP-tagged proteins coupled to D1R and D2R and their ... D1–CFP and D2–YFP (black line) (C) Cotransfection of HEK293 with D1MUT–CFP and D1–YFP (gray line) in comparison with D1–CFP and D1–YFP (black line) (D) Cotransfection of HEK293 with D2–CFP and D2R3–YFP...

Ngày tải lên: 07/03/2014, 03:20

... the current supply of and the demand for milk in Ethiopia Dairy products are traditional consumption items with strong demand, and the temperate climate of the Ethiopian highlands allows the crossbreeding ... inputs and lack of information on dairy and beef production and marketing and also the dairy and beef market are localized Therefore, improvements of dairy and meat productions in Fogera might have ... Gurage and Hadiya area Ambo Western shoa around Ambo,Dandi,Addis Alem and Holetta Ogaden Ethiopia:Ogaden area of Somalia region and bordering eastern Hararge Jem-Jem Arado/1/ Northern Shire,Adwa and...

Ngày tải lên: 08/03/2014, 23:20

... year Fifth year State Private Mean Standard deviation Mean Standard deviation Mean Standard deviation Mean Standard deviation Mean Standard deviation Mean Standard deviation Responsibility 4.3 ... subject “Business Ethics” and other free-electives as a complement and to develop ethics and social values Research is currently been done on the values of today’s society and values that have to ... has promoted ethics and social values The results of this research work will help to review the competences established in degrees and in the different course material covered, and also help to...

Ngày tải lên: 16/03/2014, 03:20

... molecular mass of holo-ACP dimer and the mixture of holo-ACP monomer and apo-ACP were found to be 33 kDa and 25 kDa, respectively Puried holo-ACP and the mixture of apo-ACP and holo-ACP monomer were ... monomer and apo-ACP with His-tag Lane 4: mixture of holo-ACP monomer and apo-ACP without His-tag (F) Separation of apo-ACP and holo-ACP by anion exchange chromatography Elution prole of apo-ACP and ... apo-ACP and holo-ACP monomers (D) Separation prole of holo-ACP dimer and apo-ACP and holo-ACP monomers: 12% native PAGE showing the separation of holo-ACP dimer from a mixture of apo-ACP and holo-ACP...

Ngày tải lên: 16/03/2014, 10:20

... enzyme [1621] as a reference Materials and methods Materials The restriction proteases enterokinase and factor Xa were obtained from Invitrogen (The Netherlands) and Protein Engineering Technology ... homogeneity from bovine heart and was a generous gift from S O Dứskeland, Department of Anatomy and Cell Biology, University of Bergen The pterin cofactors (H4biopterin, H2biopterin and H46-methylpterin) ... H4biopterin and substrate (PDB id code 1KWO) and (D) binary hPAH with bound dopamine (PDB id code 5PAH) The backbone of rPAH regulatory domain and hPAH catalytic domain are shown in red and green,...

Ngày tải lên: 17/03/2014, 09:20