activity of mtorc2 and homeostatic feedback loops
báo cáo khoa học: " Activity of enzymes and fitness variation" doc
Ngày tải lên :
09/08/2014, 22:22
... in the G-5 and G-10 generations of this selection Greater activity of a majority of enzymes was found in the fast group and, vice versa, lower activity was observed in the slow groups of flies ... presents the analyses of variances in activities of enzymes (A) between and (B) within G-1, G-5, and G-10generations of selection for fast and slow prea- dult development of D melanogaster In ... enzymes The activities of some of the studied enzymes are significantly positively associated (e.g ldh, and Me, ldh and a-Gpd, Me and a-Gpd, Adh and ldh, etc.) On the other hand, there is marginally...
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Báo cáo y học: "Antiviral activity of Engystol® and Gripp-Heel®: an in-vitro assessment" pptx
Ngày tải lên :
11/08/2014, 08:21
... The regulation of IFN production is dependent on the virus strain, the kind of infected host cell and type of IFN Multiple Toll-like receptors (TLR)-dependent (TLR-3,-4,-7 and -9) and independent ... type (Para3) and adenovirus type (Ad5) were obtained from the Friedrich-Löffler Institute (FLI) Tübingen and the Department of Medical Virology and Epidemiology of Virus Diseases of the Hygiene ... release in PBMCs and virus-susceptible cell lines, virus suspensions of inactivated HSV-1, HRV14 and FluA were employed Roeska and Seilheimer Journal of Immune Based Therapies and Vaccines 2010,...
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Báo cáo sinh học: "Bactericidal activity of oxacillin and glycopeptides against Staphylococcus aureus in patients with endocarditis: Looking for a relationship between tolerance and outcome" doc
Ngày tải lên :
12/08/2014, 17:20
... Approved Standard Clinical and Laboratory Standard Institute, Wayne, PA, 2009, M07-A8 18 Clinical and Laboratory Standard Institute: Methods for Determining Bactericidal Activity of Antimicrobial ... approved by the institutional ethical committee of Perugia and Bergamo All activity was conducted in accordance with the Declaration of Helsinki, and national and institutional review board Associations ... Annals of Clinical Microbiology and Antimicrobials 2011, 10:26 http://www.ann-clinmicrob.com/content/10/1/26 Page of Table Rates of cure, surgery, and embolism in endocarditis caused by MSSA and...
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Investigations on the antimalarial activity of alkoxylated and hydroxylated chalcones 3
Ngày tải lên :
17/09/2015, 17:18
... effects of pepstatin A and E64 on the enzymatic activity of a crude P yoelii extract They found that the breakdown of radiolabelled methemoglobin was reduced by 71% and 26% in the presence of pepstatin ... MODE OF ANTIMALARIAL ACTIVITY OF CHALCONES 6.1 Introduction The mode of action of chalcones in malaria remains uncertain As described in the Introduction (Section 1.3.1 ) the antimalarial activity ... inhibition of methemoglobin breakdown and in vitro antimalarial activity Inhibition of falcipain-2 is accompanied by visible changes in the digestive vacuole of Plasmodium, namely the appearance of swollen...
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Investigations on the antimalarial activity of alkoxylated and hydroxylated chalcones 2
Ngày tải lên :
17/09/2015, 17:18
... 100 80 % of Compounds A 60 40 20 A B C D Le ve l of activity E A B C D Level of activity E 60 40 20 A B C D Level of activity E Figure 5.8 a-d Steric and electrostatic contour maps of CoMFA models ... determinants of activity These are dipole moment, disubstitution, molar refractivity and log kw, in order of decreasing importance Of these descriptors, dipole moment and log kw influenced activity ... Determination of in vitro antileishmanial activity Antileishmanial activity is not determined by the candidate but carried out in Dr Simon L Croft’s laboratory in the London School of Hygiene and Tropical...
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Investigations on the antimalarial activity of alkoxylated and hydroxylated chalcones 1
Ngày tải lên :
17/09/2015, 17:18
... AIM OF THESIS AIM OF THESIS The aim of this thesis is to obtain a better understanding of the antimalarial activity of chalcones This is to be achieved by synthesis, evaluation of antimalarial activity, ... and there is a better understanding of the mode of action of antimalarial agents like chloroquine and artemisinin Secondly, the successful sequencing of the genome of P falciparum will undoubtedly ... the importance of the azomethine nitrogen Because of the commercial availability of isomeric quinoline aldehydes and naphthalene aldehydes, the effect of position of attachment on activity is also...
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ANALYSIS OF MICROBIAL COMMUNITY STRUCTURE AND IN SITU ACTIVITY OF NITRIFYING BIOFILMS
Ngày tải lên :
05/09/2013, 08:40
... microprofiles of O2, NH4+, NO2-, and NO3- in the autotrophic nitrifying biofilm (B) The distribution and magnitude of the estimated specific rates of net consumption and production of NH4+, NO2-, and ... organization of nitrifying bacterial populations and the in situ activity of these populations within the biofilms was discussed Microsensors For analysis of microbial structure and function (activity) of ... only net chemical profiles, and the spatial resolution is also above a single-cell level To address the question of the higher abundance and activity of SRB in oxic zones of biofilms (Okabe et al.,...
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Tài liệu LoopStar® 711 Leveraging a Full Suite of Ethernet and TDM Services to Cost-Effectively Utilize Fiber Networks doc
Ngày tải lên :
10/12/2013, 19:15
... LoopStar® 711 Leveraging a Full Suite of Ethernet and TDM Services to Cost-Effectively Utilize Fiber Networks Ethernet and Legacy Services for True Convergence Providers of all sizes and ... CLI management interfaces - RS-232 - 10/100 • In-band or out -of- band • SNMP • FTP to download multiple software images and configurations • LoopStar 700 intelligent management Ethernet Services ... LoopStar 711 allows providers to leverage and extend their footprint by delivering Ethernet and TDM-based services over existing fiber connections w w w a d c c o m • + - - - 8 • - 0 - 6 - LoopStar®...
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Tài liệu Báo cáo khoa học: The ubiquitin ligase Itch mediates the antiapoptotic activity of epidermal growth factor by promoting the ubiquitylation and degradation of the truncated C-terminal portion of Bid ppt
Ngày tải lên :
16/02/2014, 09:20
... between EGF-induced reduction in Bid and tBid levels and the ubiquitin ligase activity of Itch In this study, we first examined the ability of Itch to interact with Bid and tBid We found that Itch specifically ... inset) n = conditions [29] Treatment of HEK-293T cells with TRAIL is known to induce caspase activity and cleavage of Bid in tBid [30] In our hands, treatment of HEK-293T cells with 200 ngÆmL)1 ... ± 0,02% of control; P = 0.001 for cell survival and 0.94 ± 0.03 of control for caspase activity) , and treatment with EGF slightly increased this effect on cell survival (88.4 ± 2.0% of control;...
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Tài liệu Báo cáo khoa học: Structural effects of a dimer interface mutation on catalytic activity of triosephosphate isomerase The role of conserved residues and complementary mutations pptx
Ngày tải lên :
18/02/2014, 11:20
... selection of recombinants The sites incorporated were HaeIII, NcoI and BamHI (Table 1) The PCR mix contained 200 ng of each primer, 20 ng of the template, 200 lm of each dNTP and units of Taq DNA ... subunit dissociation [27] and also in maintaining the geometry of the active site The availability of crystal structures of TIMs from 21 sources and the large database of TIM sequences from various ... 5.5 and w = )144.1 7.0 [53] The distribution of the / and w values of all other Lys residues in the TIM structure is shown for comparison The possible role of energetically unfavorable Ramachandran...
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Tài liệu Báo cáo khoa học: Structural insights into the substrate specificity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria ppt
Ngày tải lên :
18/02/2014, 16:20
... understanding of the generic principle of catalysis for this family of proteases Results and Discussion Fold of ervatamin-A The overall structure and fold of ervatamin-A is similar to that of ervatamin-B, ... [27,35] and other members of the papain family, and is made up of two domains, L and R, with a V-shaped active site cleft at the interface of the domains (Fig 1) Substrate specificity and activity of ... investigation of the catalytic mechanism and substrate specificity and to understand the differences therein for this class of enzymes In order to identify the subsites of the ervatamins and to understand...
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Tài liệu Báo cáo khoa học: The effect of small molecules in modulating the chaperone activity of aB-crystallin against ordered and disordered protein aggregation pdf
Ngày tải lên :
18/02/2014, 16:20
... Electron micrographs of a-synucleinA53T at the end of the assay confirmed the formation of fibrils, which were long (between and nm), straight and unbranched (Fig 6C,D) Addition of Gly and Lys-HCl at ... the effect of various additives and ⁄ or modifications on the overall activity of chaperone proteins Of the target proteins tested, Arg-HCl was found to specifically increase the activity of aB-crystallin ... no influence of the additive on the chaperone activity of aB-crystallin (i.e the effect of the additive on the aggregation of the target protein is independent and different from that of the chaperone)...
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Tài liệu Báo cáo khoa học: DNA strand exchange activity of rice recombinase OsDmc1 monitored by fluorescence resonance energy transfer and the role of ATP hydrolysis pptx
Ngày tải lên :
19/02/2014, 07:20
... strand exchange activity of OsDmc1 by FRET Strand exchange activity of OsDmc1 Protein concentration dependence Fig Renaturation activity mediated by OsDmc1 protein in the presence of ATP and ... between donor and acceptor dyes, will therefore unveil the status of physical union and separation of complementary strands during renaturation and strand exchange, respectively, and thereby assesses ... single and double stranded DNA binding activity Binding to single stranded DNA stimulated a significant level of DNA dependent ATPase activity OsDmc1 also showed renaturation of complementary strands...
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Tài liệu Báo cáo khoa học: Oxidized elafin and trappin poorly inhibit the elastolytic activity of neutrophil elastase and proteinase 3 pdf
Ngày tải lên :
19/02/2014, 07:20
... of increasing concentrations of native and oxidized elafin and trappin on the activity of a constant concentration of NE and kdiss Ki ¼ kdiss ⁄ kass [5,8] Trappin is a 9.9-kDa protein formed of ... of elafin and trappin oxidation on the affinity of the inhibitors for NE is caused by 5886 Elafin and the inhibitory domain of trappin each have two methionine residues (M25 and M51 for elafin, and ... results of competition experiments carried out with 5887 Inhibition of elastase and proteinase by elafin S M Nobar et al Table Theoretical and observed inhibition of the elastolytic activity of neutrophil...
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Tài liệu Báo cáo khóa học: The effect of mutations surrounding and within the active site on the catalytic activity of ricin A chain pptx
Ngày tải lên :
19/02/2014, 12:20
... affecting the activity of ricin A chain (Eur J Biochem 271) 159 Fig Assessment of the N-glycosidase activity and cytotoxicity of RTA N78S and ricin N78S (A) N-glycosidase activity of RTA N78S Isolated ... a total volume of 20 lL Reactions were stopped by the addition of 100 lL of 2· Kirby buffer [25] and 80 lL of H2O, and rRNA was obtained by precipitation after two phenol– chloroform extractions ... IMAGEQUANT software, and depurination was calculated by relating the amounts of the small aniline-fragment and 5.8S rRNA and expressing values as a percentage Reassociation and quantification of ricin...
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Tài liệu Báo cáo khoa học: Design, structure and biological activity of b-turn peptides of CD2 protein for inhibition of T-cell adhesion ppt
Ngày tải lên :
19/02/2014, 13:20
... bonds between NH of Thr5 and CO of Arg2, as well as NH of Arg2 and CO of Thr5 Structure of cyclic hexapeptides – cYT peptide The NMR data of the cYT peptide were indicative of its flexible nature ... intensity of ROE cross-peaks, and were assigned as strong, medium and weak ˚ represent the family Based on ROE violation > 0.2 A and allowed values of /, w in the Ramachandran map, only one family of ... percentage inhibition of peptide-treated cells and expressed as the mean of three independent experiments activity of 40% at 100 lM concentration Both linear and cyclic ER peptides (lER and cER) showed...
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Tài liệu Báo cáo khoa học: The role of N-glycosylation in the stability, trafficking and GABA-uptake of GABA-transporter 1 Terminal N-glycans facilitate efficient GABA-uptake activity of the GABA transporter pptx
Ngày tải lên :
19/02/2014, 17:20
... half-lives of 22 and 23 h for wild type GAT1 in the absence and presence of dMM, respectively, and of 12 and 5.5 h for the DDN and DDQ mutants, respectively GABA values of NNN treated with dMM, and of ... GABA-uptake activity of NNN 1631 Role of N-glycosylation and N-glycan trimming of GAT1 G Cai et al Fig Kinetic analysis of GABA-uptake by GFP-tagged GAT1 wild type (NNN) with and without dMM and N-glycosylation ... activities of the N-glycosylation 1628 Fig Determination of expression of GFP-tagged GAT1 mutants on the surface of transfected CHO cells and measurement of GABA-uptake by GFP-tagged GAT1 wild type and...
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Tài liệu Báo cáo khoa học: Adaptive changes in the expression of nuclear and mitochondrial encoded subunits of cytochrome c oxidase and the catalytic activity during hypoxia pptx
Ngày tải lên :
20/02/2014, 23:20
... switch and alters the expression of the two nuclear genome coded isoforms of CytOX V [10,32] The regulation of genes CytOX5a and CytOX5b, coding for the two isofoms Va and Vb, parallels that of genes ... Effect of hypoxia on CytOX activity, mitochondrial heme aa3 content and TN (s)1) in macrophages and PC12 cells CytOX activity in submitochondrial particles (SMP) from standard (141 Torr O2) and ... stages of adaptive response to hypoxia Based on the results of our study and those of Schumacker, it is reasonable to conclude that the effects of hypoxia on CytOX gene expression and its activity...
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Tài liệu Báo cáo khoa học: Molecular characterization and allergenic activity of Lyc e 2 (b-fructofuranosidase), a glycosylated allergen of tomato pdf
Ngày tải lên :
21/02/2014, 00:20
... specific 5¢ -and 3¢ primers selected on the basis of the published sequences for tomato b-fructofuranosidase (GenBank accession no D11350 and S70040) Primers for the short isoform of b-fructofuranosidase ... of 50 kDa would suggest that we had purified the truncated isoform Cloning of the cDNA of two isoforms of tomato b-fructofuranosidase and recombinant expression in E coli For protein expression ... reactivity of some sera to a 9- and a 15-kDa band We could show that the 9-kDa band in the tomato extracts reacts with a specific antibody against the LTP from cherry, Pru av and the 15-kDa band...
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Tài liệu Báo cáo khoa học: Physicochemical characterization and biological activity of a glycoglycerolipid from Mycoplasma fermentans ppt
Ngày tải lên :
21/02/2014, 00:20
... presence of minute amounts of endotoxins The comparison of LPS and MfGl-II in the LAL assay shows, as expected, activity of LPS in the range of down to 10 pgÆmL)1, whereas MfGl-II has only activity ... respectively [23,30], and the bands at Fig Infrared spectrum in the spectral range 1800–900 cm)1 (A) and – in the range of the negatively charged phosphate band mas (PO2 ) 1300– )1 1190 cm (B) of hydrated ... water-binding capacity of lecithin headgroups [30] Finally, MfGl-II exhibits a main band at 1245 cm)1 and further weak bands at 1220 and 1260 cm)1 Thus, the phosphate groups of this glycolipid are...
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