activity of crude salivary gland extracts 50

... Anticoagulant activity of ticks 50 3.1.1 Protein quantification of crude salivary gland extracts 50 3.1.2 Activity of crude salivary gland extracts 50 3.1.3 Purification of salivary gland extracts ... Anticoagulant activity of crude R pulchellus salivary gland extracts 51 Figure 3.2 Anticoagulant profile of R pulchellus SGEs 52 Figure 3.3 Anticoagulant profile of D reticulatus ... 2.1 Salivary glands and extracts Salivary gland and salivary gland extracts of ticks were obtained from collaborators RNDr Mirko Slovak and RNDr Maria Kazimorova from the Slovak Academy of Sciences,...

Ngày tải lên: 09/09/2015, 08:13

... analysis of a T infestans salivary gland extract and puried recombinant triafestin-1 and triafestin-2 A crude salivary gland extract (lane 1), lg of recombinant triafestin-1 (lane 2) and lg of recombinant ... and expression of recombinant triafestin-1 and triafestin-2 A cDNA library was constructed from the salivary glands of unfed fth instar nymphs of T infestans cDNA fragments from 550 clones were ... activation of FXII and PK FXII was preincubated with various concentrations of triafestin-1 or triafestin-2 for 10 The activation reaction was started by addition of PK and DS 500 The total activity of...

Ngày tải lên: 16/03/2014, 05:20

... of T infestans saliva Fig Western blot analysis of triplatin-1 and -2 from the salivary gland of T infestans Extracts from a pair of salivary glands, recombinant triplatin-1 and recombinant triplatin-2 ... Isolation and sequencing of cDNA clones Salivary glands of T infestans were dissected from thoraces of unfed adults, and poly A(+) RNA was isolated from 30 pairs of salivary glands using a MicroPrep ... Biochemistry 67, 143– 150 Keller PM, Schultz LD, Condra C, Karczewski J & Connolly TM (1992) An inhibitor of collagen-stimulated platelet activation from the salivary glands of the Haementetia of cinalis...

Ngày tải lên: 30/03/2014, 10:20

... homozygous double congenic strain The purpose of the present study was to monitor the influence of each of these two loci on the incidence and severity of sialadenitis, and to identify genes located ... samples included high amounts (from to μg/μl) of good quality RNA Salivary gland gene expression The total RNA was submitted to the RNA-in/data-out service of the Norwegian Microarray Consortium Core ... significance level of 0.05 For each assay, one reaction without Multiscribe RT enzyme was included, to ensure RNA specificity of the assays Results Influence of NOD Idd5 and Nss1 loci on severity of sialadenitis...

Ngày tải lên: 09/08/2014, 10:21

... Diseases of the salivary glands Inflammatory salivary gland diseases, next to benign neoplasms, are the most frequent causes of salivary gland swelling in juvenile age [3] The acute forms of sialadenitis ... to Eneroth [13] salivary gland tumors make up 0.3% of all human tumors, and less than 10% of all juvenile head and neck tumors are located in the salivary glands [14] Only 1% of all head and ... instance of persisting postoperative nerve injury or symptomatic Frey’s syndrome following total extirpation of the parotid gland due to chronic sialadenitis Page of Tumors of the salivary glands...

Ngày tải lên: 11/08/2014, 20:20

... differentiation of secretory acinus and related ducts, the functional unit of salivary glands Immunohistochemical studies demonstrated different degrees of EGFR expression in several salivary gland carcinomas, ... the antitumor activity of anti-EGFR targeted agents in the salivary gland neoplasms In a phase II study of cetuximab monotherapy in 22 patients with recurrent/metastatic salivary gland carcinomas, ... palliative management of advanced salivary gland cancers J Clin Oncol 2006, 24:2673-2678 Agulnik M, Siu LL: An update on the systemic therapy of malignant salivary gland cancers: role of chemotherapy...

Ngày tải lên: 11/08/2014, 21:22

... the presence of proteins able to bind to the four DENV serotypes in mosquito salivary gland extracts Because SGE might contain both salivary gland tissue (basal lamina or salivary gland epithelial ... phenylmethylsulfonyl fluoride and 10 μg/ ml of aprotinine) Each vial contained about 1 ,500 pairs of salivary glands and was stored at -80°C until needed [8] Salivary glands were then thawed and disrupted ... monoclonal polynesiensis salivary glands DENV-binding proteins from Ae polynesiensis salivary glands detected with anti-E monoclonal antibodies Ae polynesiensis salivary gland extracts were treated as...

Ngày tải lên: 12/08/2014, 04:21

... currently professor of nutritional biochemistry in the Department of Nutrition and Dietetics, King’s College London and honorary professor of clinical biochemistry in the Department of Clinical ... disruption of mitochondrial membrane potential, activation of multiple caspases, arrest of cell cycle progression, and induction of differentiation The positive effect of this compound on other types of ... survival The ability of SFN and PEITC to inhibit the transcriptional activity of NF-κB is a consequence of the phytochemicals antagonizing phosphorylation of IκB, the inhibitor of NF-κB, which is...

Ngày tải lên: 14/03/2014, 10:20

... found in the salivary glands To detect protrialysin stored in the salivary glands, and determine whether it would retain lytic activity, glands were extracted in the presence, or absence, of APMSF, ... M Martins et al using twofold dilutions of salivary glands extracts, or stock solutions of the peptide proP7 Recombinant protein expression and purification An NL region of preprotrialysin (spanning ... amounts of salivary gland homogenates prepared in the presence (d) or absence (s) of APMSF In (A) the number of surviving cells was determined in a hemocytometer, and the percentage of lysis...

Ngày tải lên: 16/03/2014, 06:20

... the salivary glands of patients with SS There is clear evidence of an inflammatory environment with presentation of self-antigens characteristic of SS [22] that may permit the production of autoantibodies ... the CDR of VH and VL gene rearrangements of B cells obtained by minor salivary gland biopsies from two patients with SS [6] Detailed analyses of the frequency of occurrence and mutations of the ... each codon of productively rearranged VL genes of B cells isolated from peripheral blood and from the parotid gland of a patient with Sjögren’s syndrome The frequency of mutation of each codon...

Ngày tải lên: 09/08/2014, 03:24

... pathogenesis of RA after fractalkine and CX3CR1 expression were reported to be upregulated in the synovium of patients with RA [9] Supporting the notion of the disease-modulating activity of fractalkine ... Pichardo EL, Shah N, Peck AB, Humphreys-Beher MG: Elevated levels of cysteine protease activity in saliva and salivary glands of the nonobese diabetic (NOD) mouse model for Sjögren syndrome Proc ... profile but not with histopathologic changes in the salivary gland Arthritis Rheum 2006, 54:23002305 Delaleu N, Immervoll H, Cornelius J, Jonsson R: Biomarker profiles in serum and saliva of...

Ngày tải lên: 09/08/2014, 10:23

... negative in primary adenocarcinomas of the lung, a salivary gland origin was most likely Immunohistochemistry might also aid in the differential diagnosis of salivary gland carcinoma types In the present ... incidence of the adenocarcinoma (NOS) is decreasing with the establishment of new neoplastic entities of the salivary glands, this carcinoma still occurs and should be taken into account in case of ... Adenocarcinoma of salivary origin: Clinicopathologic study of 204 patients Am J Surg 1982, 144:423-31 Matsuba HM, Mauney M, Simpson JR, Thawley SE, Pikul FJ: Adenocarcinomas of major and minor salivary gland...

Ngày tải lên: 11/08/2014, 20:20

... Myoepithelioma is a rare benign tumor of the salivary glands and is usually seen in the parotid gland and the minor salivary glands It was once considered to be a type of pleomorphic adenoma (PA), but ... tumor is usually located in the parotid gland and the minor salivary glands of the soft palate and represents less than 1% of all salivary gland tumors [2] Several authors now consider this tumor ... epithelial neoplasms In Tumors of Salivary Glands AFIP; 2002: 57-68 Ferri E, Pavon I, Armato E, Cavaleri S, Capuzzo P, Ianniello F: Myoepithelioma of a minor salivary gland of the cheek: case report...

Ngày tải lên: 11/08/2014, 20:21

... with tumor-like lesions of the salivary glands at the outpatient care of an ENT-department (university hospital) 30.7% of the patients with tumor-like lesions in salivary glands underwent an imaging ... sensitivity of 98%, a specificity of 52% and an accuracy of 84% According to Takashima et al., MRI achieved a sensitivity of 60%, a specificity of 88% and an accuracy of 81% in the assessment of tumor ... Adenoma of the left parotid gland Pleomorphic Adenoma of the left parotid gland: Ultrasound image axial and Ultrasound image transversal ultrasound head or located deeper in the salivary gland, ...

Ngày tải lên: 11/08/2014, 23:22

... 1C) The effect of the length of the incubation period on the rate of inhibition of the HIV-1 infection of the CEM Table Mucin toxicity Sample Con CEM SS cells % of dead cells % of live cells MUC5B ... of the salivary glands which results in a decline in the amount of saliva and a change in its constituents [10] This in turn may affect the glycosylation pattern or sugar composition of the salivary ... immunoreactivity differences between salivary MUC5B and MUC7 mucins Habte et al Virology Journal 2010, 7:269 http://www.virologyj.com/content/7/1/269 Page of Figure Anti-HIV-1 activities of salivary...

Ngày tải lên: 12/08/2014, 01:22

... to estimate activity of the RAS with a greater decrease in SBP connoting augmented system activity [19] Serum ACE Activity: ACE refers to the angiotensin converting enzyme Serum ACE activity was ... dose of 10 mg/kg Each rat received a single dose of LNAME Measurements of SBP were taken at four, seven, 10, 15, and 20 minutes post injection The area under the curve was used to estimate activity ... initiated on day 338 in the life of the female SD, the SBP of all 40 SD ingesting the control diet containing 20% sucrose had been recorded previously starting on day 50 of life up to that point in...

Ngày tải lên: 26/10/2012, 08:57

... antioxidant extracts, each of which has been claimed to exert chemopreventive activity in cellular models of cancer [43,44] Recent publications have stated that the antiproliferative activity of flavonoids ... Polyphenolic fractions at IC50 Polyphenolic fractions at IC50 Fig Scavenging activity of OH and OÁÀ analyzed by ESR Grape and pine fractions were evaluated at: (A) 50 lM and (B) IC50 in HT29 cells in ... properties of natural extracts D Lizarraga et al Table Comparative chemical characteristics and HT29 cell growth inhibition of grape and pine fractions Percentage of galloylation (%G), mean degree of...

Ngày tải lên: 07/03/2014, 05:20

... respectively, in isoform A of SAL, the structure of which is described here, and are replaced in isoform B by Ala and Val, respectively Replacement of residues 43 and 75 with those of isoform B in the ... OBP of  441 A3, hamster ˚ OBP of  500 A ˚ aphrodisin  350 A3, and horse allergen (Fig 5B), has the ˚ smallest,  273 A3 The small size of the main cavity in SAL may be due to the presence of ... periphery of the protein (Fig 5A) Unexpectedly, the volume of the main cavity is one of the smallest among lipocalins of known three-dimensional structures The ˚ cavity of MUP has a volume of  510...

Ngày tải lên: 17/03/2014, 17:20

... anticoagulants of H longicornis It was previously reported that anticoagulants are present in the salivary glands of H longicornis [24] According to this report, an extract of the salivary glands prolonged ... Chemical Industry, Osaka, Japan Mass sequence analysis of cDNA clones H longicornis salivary gland mRNA was isolated from 10 pairs of glands of ticks at three distinct feeding stages (unfed, slow ... mechanism of action of human antithrombin-heparin cofactor J Biol Chem 248, 6490– 6505 14 Tollefsen, D.M., Pestka, C.A & Monafo, W.J (1982) Heparin cofactor II Purification and properties of a heparin-dependent...

Ngày tải lên: 31/03/2014, 01:20

... concentrations, solution of 50 ll was pipetted into the 96 orifice plate and followed by addition of 150 ll DPPH reagent ( 250 lL DPPH per liter methanol) After 30 min, the absorbance of the mixture at ... the conversion of the Au(III), X the value of any of the five parameters of each broth, Cov(X, Y) the covariance value and rxy the correlation coefficient of X and Y with a range of [-1, 1], E the ... Technologies Holdings Co Ltd.) Mixture of ethanol solution ( 150 ll) and the broth (50 ll) served as the blank and DPPH solution ( 150 ll) plus ethanol (50 ll) as the control The DPPH radical scavenging...

Ngày tải lên: 21/06/2014, 17:20