activities tdc and str gene expression and catharanthine accumulation are inhibited by suramin

báo cáo khoa học: " LysoPC acyltransferase/PC transacylase activities in plant plasma membrane and plasma membrane-associated endoplasmic reticulum" ppt

Ngày tải lên : 12/08/2014, 05:20

... bar) and free fatty acids (diagonally hatched bar) is based on the data from one representative experiment and mean values and the range of duplicates are presented FM4-64, and observed by confocal ... membrane distribution, the panels A and B present the results from two independent experiments, with the plasma membrane marker and the protein distribution analyzed for both (cf Figs 1A and 3) the ... A, Distribution of radiolabel between 18:1-CoA (solid squares), free fatty acids (open squares) and phospholipids (open circles) B, Incorporation of radiolabel into PC (filled symbols) and PE...

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Tài liệu Báo cáo khoa học: Electrical properties of plasma membrane modulate subcellular distribution of K-Ras ppt

Ngày tải lên : 19/02/2014, 00:20

... order to evaluate expression and subcellular distribution of these proteins, CHO-K1 cells were transiently transfected with corresponding DNA constructs, and expression was monitored by western blot ... (S) and the pellet (P) was resuspended in buffer and centrifuged twice Soluble fractions after washing were recovered (W1 and W2) YFP-K-RasC14 expression in W1, W2 and P fractions was analyzed by ... are necessary for proper membrane binding and subcellular distribution of K-Ras Calcium ionophore redistributes K-Ras to endomembrane In vitro experiments in this study and others have demonstrated...

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Báo cáo Y học: In vivo activation of plasma membrane H+-ATPase hydrolytic activity by complex lipid-bound unsaturated fatty acids in Ustilago maydis docx

Ngày tải lên : 08/03/2014, 22:20

... identical to a general mechanism for inhibition except that, in this case, the enzyme is inhibited by the absence and not by the presence of the activator (A) If KSs ¼ KSm and KAs ¼ KAm and ¼ k < ... Stress activation of H+-ATPase by fatty acids (Eur J Biochem 269) 1007 Ó FEBS 2002 Table Relevant biological characteristics of U maydis strains and treatments Genetic lesions and sterol ... for substrate decreases from 2.1 mM to 0.8 mM and the inhibitory effect of vanadate is augmented by up to ﬁvefold; similar changes are observed for Pma2p [34] On the other hand, salt stress in...

6
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Báo cáo khoa học: Membrane compartments and purinergic signalling: the role of plasma membrane microdomains in the modulation of P2XR-mediated signalling pot

Ngày tải lên : 30/03/2014, 02:20

... cloned and characterized both functionally and pharmacologically [5] Two main classes of purinergic receptors are well characterized, i.e P1 receptors which bind adenosine, and P2 receptors which are ... receptors are widely distributed across different tissues and organs [10] They are expressed in both excitable cells (such as neurons) and non-excitable cells (such as epithelial and immune cells) ... bulk of ﬂuid membrane and which are enriched in cholesterol and (glycero-)sphingolipids In addition the fatty acid chains of the phospholipids composing these rigid domains are generally more saturated...

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Báo cáo khoa học: Phosphatidylserine induces functional and structural alterations of the membrane-associated pleckstrin homology domain of phospholipase C-d1 docx

Ngày tải lên : 30/03/2014, 10:20

... Glu85, and Lys86) are indicated by red circles, and hydrophobic residues (Leu84, Phe87 and Ala88) are indicated by blue circles The white circle indicates Gly83 IP3 and PIP2 head groups are shown by ... are shown by blue and yellow hexagons, respectively The head groups of PC and PS are shown by yellow and red circles, respectively Cations and anions in (D) are shown by cyan and orange circles ... are as follows: (a) with IP3 in solution (structure I); (b) with PIP2 embedded in PC ⁄ PIP2 vesicle (structure II); and (c) with PIP2 embedded in PC ⁄ PS ⁄ PIP2 vesicle (structure III), and are...

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Báo cáo hóa học: "Research Article Epileptic Seizure Prediction by a System of Particle Filter Associated with a Neural Network" doc

Ngày tải lên : 21/06/2014, 20:20

... Processing where xk is a random variable and has a normal distribution initially vk , wk are white noise with Gaussian distribution and they are independent α, β are parameters to be determined Ek ... non-Gaussian distributions, and they are improved greatly in the implementation [12–17] Particle filters can approximate a sequence of probability distributions of interest using a set of random samples ... the energy of specific band since it can give good performance and it is widely used to analyze EEG data Compared with energy of different frequency bands, the frequency band of 4–12 Hz shows much...

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Báo cáo lâm nghiệp: "-pumping + H activities of plasma membrane and tonoplast from Quercus rubra roots" pptx

Ngày tải lên : 09/08/2014, 04:20

... consisted of vesicles and most of them were not stained by phos photungstic-acid-CrO The ATPase activity (Table II) was stimulated by CI-, inhibited by N0 (60%) and slightly inhibited by vanadate (plasma ... stimulated by CI-, inhibited by N0 and relatively insen3 sitive to cations and vanadate; whereas the other in the high density membrane fraction is stimulated by K+, relatively insensitive to anions and ... suggest a partial contamination by plasma membranes Vanadate and nitrate have similar effects on ATPase and H (Figs -pumping + and 2) Thus the H+-pumping observed is driven by ATP hydrolysis The difference...

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báo cáo khoa học: "Prozone effect of serum IgE levels in a case of plasma cell leukemia" pot

Ngày tải lên : 10/08/2014, 22:21

... actual instrument reading (counts per second) along the Y axis Both axes are logarithmic and the dilutions that were used were 1:100,000, 1:10,000, 1:1000, 1:100, and undiluted In parenthesis ... point is the reading reported by the instrument The dotted line represents the highest reported value of 2000 IU/mL The lines connecting each point are for illustration and not represent the actual ... for SPEP and serum immunotyping was performed by the Capillarys capillary method (Sebia Electrophoresis, Norcross GA) Serum IgE levels were measured by the Siemens Immulite 2000® (Flanders NJ),...

3
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Báo cáo y học: "Genome-wide analysis of primary CD4+ and CD8+ T cell transcriptomes shows evidence for a network of enriched pathways associated with HIV disease" pot

Ngày tải lên : 13/08/2014, 01:20

... this gene set and those genes appear before or at the peak are defined as core enrichment genes for this gene set B Heat map of the genes within the gene set of TCA cycle corresponding to A The genes ... genes from human were used as the reference gene set GO categories with at least genes and p < 0.01 are identified as enriched and colored red in the GOTree In GOTree, O stands for observed gene ... Figure Gene ontology (GO) tree and bar chart for the enriched GO categories GO categories with at least genes and p < 0.01 are identified as enriched and colored red in the GOTree In GOTree, O stands...

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Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc

Ngày tải lên : 19/02/2014, 07:20

... thioredoxin gene, using NdeI and XhoI restriction sites The resulting plasmid was transferred into E coli strain XL10 and the frame and the identity of the cloned fragment veriﬁed by sequencing The construct ... ([1] and references therein) The auto-inhibitory action of the C-terminus is suppressed, besides by pH, by lysophospholipids and by 14-3-3 proteins ([1] and references therein) The latter are ... Escherichia coli XL10 (Stratagene, La Jolla, CA, USA) was used for recombinant DNA work while BL21(DE3)pLysS (Novagen, Madison, WI, USA) and BL21(DE3) Codon plusTM pRil strains (Stratagene) were employed...

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Báo cáo khoa học: Epoxidation of benzo[a]pyrene-7,8-dihydrodiol by human CYP1A1 in reconstituted membranes Effects of charge and nonbilayer phase propensity of the membrane pot

Ngày tải lên : 08/03/2014, 10:20

... (–)-7,8-diol Ole2PtdCho Ole2PtdCho/PtdSer (2 : 1) Ole2PtdCho/PtdIns (2 : 1) PtdCho/PtdEtn/PA (2 : : 0.06) Ole2PtdCho/Ole2PtdEtn (2 : 1) Ole2PtdCho/Ela2PtdEtn (2 : 1) ( )-7,8-diol + Ole2PtdCho Ole2PtdCho/PtdSer ... prepared from neutral Ole2PtdCho and mixtures of Ole2PtdCho and anionic lipids (PtdSer, PtdIns, PA) PtdSer is the most frequently occurring negatively charged microsomal lipid, whereas PtdIns and ... the striking parallelism of the curves in the Lineweaver–Burk plots with different lipid components (i.e for Ole2PtdCho, Ole2PtdCho/Ela2PtdEtn, and Ole2PtdCho/Ole2PtdEtn) indicates that Km and...

7
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Báo cáo khoa học: A hydrophilic cation-binding protein of Arabidopsis thaliana, AtPCaP1, is localized to plasma membrane via N-myristoylation and interacts with calmodulin and the phosphatidylinositol phosphates PtdIns(3,4,5)P3 and PtdIns(3,5)P2 pptx

Ngày tải lên : 23/03/2014, 07:20

... study has suggested the constitutive expression and signiﬁcant stimulation of gene expression by a pathological elicitor (ﬂagellin peptide) and copper [12] In general, N-myristoylation provides ... membrane and soluble fractions To determine the amount of PCaP1 in tissues and the distribution of PCaP1 in the membrane and soluble fractions (by an immunochemical method), we prepared the recombinant ... difference between the calculated and apparent size may be caused by the amount of dodecyl-sulfate bound to PCaP1 and ⁄ or the structure in SDS Immunoblotting showed bands in Raphanus sativus (radish,...

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Báo cáo Y học: Isolation and characterization of MUC15, a novel cell membrane-associated mucin pot

Ngày tải lên : 24/03/2014, 04:21

... representation of the human MUC15 gene Nucleotide positions (in AJ417818) are indicated by numbers Exons and introns are indicated by E and I, respectively Intron sizes are given in parentheses Shaded boxes ... into pCR 2.1-TOPO cloning vectors and sequenced on both strands MUC15 expression analysis MUC15 mRNA expression was examined in a variety of tissues and cell types by PCR screening The screening ... been isolated and sequenced, thereby identifying a novel human transmembrane mucin gene named MUC15 by appointment of the HUGO Gene Nomenclature Committee Presence of O-glycosylation and sites of...

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Báo cáo hóa học: " A predominance of R5-like HIV genotypes in vaginal secretions is associated with elevated plasma HIV-1 RNA levels and the absence of anti-retroviral therapy" docx

Ngày tải lên : 20/06/2014, 01:20

... identify viral and clinical factors associated with vaginal X4- or R5-like genotypes, we evaluated plasma and vaginal viral levels, ART use, and CD4+ T lymphoctye counts using Chi-square and Fisher's ... data analysis, and drafted the manuscript PK participated in the study design and conducted the statistical analyses RC participated in the design and coordination of the study and facilitated ... samples NL processed clinical samples and conducted PCR and viral load analyses AA participated in the coordination of the study, directed sample and data analysis, and assisted in drafting the manuscript...

4
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báo cáo khoa học: "Trichoderma viride cellulase induces resistance to the antibiotic pore-forming peptide alamethicin associated with changes in the plasma membrane lipid composition of tobacco BY-2 cells" potx

Ngày tải lên : 11/08/2014, 11:21

... between CM cells and control are denoted with * for p < 0.05 and *** for p < 0.001 are product -inhibited by cellobiose, while the b-glucosidase is product -inhibited by glucose [34,35] Because of ... treatment for PS+PI and PE+PG were significantly different (p < 0.05) PS and PI are negatively charged phospholipids (at neutral pHs) as are phosphatidic acid (PA) and PG, whereas PE and phosphatidylcholine ... to Sommarin and Sandelius [82] and fractionated into neutral lipids, glycolipids and phospholipids by solid phase extraction (SPE) as described [83] For quantification of sterols and phospholipids,...

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báo cáo khoa học: " The PTI1-like kinase ZmPti1a from maize (Zea mays L.) co-localizes with callose at the plasma membrane of pollen and facilitates a competitive advantage to the male gametophyte" doc

Ngày tải lên : 12/08/2014, 05:20

... (B) Genomic locus and restriction map of the ZmPti1a gene Exons are indicated as boxes with Roman numerals Start and stop of the open reading frame are marked with an arrow and asterisk, respectively ... phosphinothricin and 0.1% (v/v) Tween® 20 10 11 12 13 14 Authors' contributions MMH, JK and SP carried out the cloning of ZmPti1 sequences and plasmid constructs and performed expression analyses MMH and ... protein expression and kinase activity studies SP and JK carried out transient localization studies MMH, SP, and JK accomplished genetic and molecular analyses of transgenic lines RL and SP carried...

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Báo cáo y học: "A prospective observational study of the relationship of critical illness associated hyperglycaemia in medical ICU patients and subsequent development of type 2 diabetes"

Ngày tải lên : 25/10/2012, 10:02

... Careis available article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses /by/ 2.0), which permits unrestricted use, distribution, and ... A, Bistrian BR: Stress-induced hyperglycemia Crit Care Clin 2001, 17:107-124 13 Grimble RF: Inflammatory status and insulin resistance Curr Opin Clin Nutr Metab Care 2002, 5:551-559 14 Marette ... results and wrote the manuscript AVB and VG participated in patients' inclusion and follow-up IPR was involved in the analysis of results and writing the manuscript All the authors read and approved...

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Treatment of Textile Wastewater by a Coupling of Activated Sludge Process with Membrane Separation

Ngày tải lên : 05/09/2013, 09:08

... pollutants and color has to be established The CASP has several disadvantages including high sludge production and large area requirement In addition, the performance of CASP is strongly affected by ... On the other hand, conventional activated sludge processes (CASP) have been widely used for treating textile wastewater To meet the stricter effluent standard, a more effective and efficient ... velocity and pressure [Wenten, 1995] The backflush was dialed with a high frequency and extremely short time duration (backshock) and combined with the use of reverse asymmetric membrane structures...

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Tài liệu Báo cáo khoa học: P25a ⁄ TPPP expression increases plasma membrane presentation of the dopamine transporter and enhances cellular sensitivity to dopamine toxicity pptx

Ngày tải lên : 14/02/2014, 21:20

... immunoreactivity demonstrating equal loading Lanes marked by DAT and MOCK represent cells transfected with DAT expression vector and empty control vector (B) Cells transfected as in (A) were analyzed by confocal ... uptake by translocating intracellular DAT to the plasma membrane strate a folded central core and unfolded N- and C-terminal extensions of 45 and 70 amino acid residues, respectively We constructed ... the norepinephrine and serotonin transporters (NET and SERT), which share a range of structural characteristics The expression of a-syn has been shown to decrease the membrane expression of all...

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