active transport a potential mechanism for specific structures

... UK) Statistical analysis All data were expressed as the mean of the individual experiments ± the standard error of the mean Data were analysed using one-way or two-way analysis of variance with ... RNeasy kit (Qiagen Ltd, Crawley, UK) Total RNA was separated on a 1% agarose/formaldehyde gel, transferred overnight to Hybond-N nylon membranes (Amersham Biosciences) and was analysed by specific ... Capell HA, Sattar N: Trial of Atorvastatin in Rheumatoid Arthritis (TARA): double-blind, randomised placebo-controlled trial Lancet 2004, 363:2015-2021 Palmer G, Chobaz V, Talabot-Ayer D, Taylor...

Ngày tải lên: 09/08/2014, 08:22

... Monetary and Financial Analysis Department Bank of Canada Ottawa, Ontario, Canada K 1A 0G9 jattamensah@bankofcanada.ca The views expressed in this paper are those of the author No responsibility for ... Ottawa, Ontario K 1A 0G9 E-mail: publications@bankofcanada.ca Web site: http://www.bankofcanada.ca Diffusion des publications, Banque du Canada 234, rue Wellington, Ottawa (Ontario) K 1A 0G9 Adresse ... Bank of Canada Working Papers Documents de travail de la Banque du Canada Working papers are generally published in the language of the author, with an abstract in both official languages Les...

Ngày tải lên: 16/02/2014, 02:20

... of anti-placental alkaline phosphatase-agarose (anti-PLAP; Sigma) was packed into an FPLC column (Amersham Biosciences, Chalfont St Giles, UK) Purification of FN3d–AP was carried out using an AKTA ... Frisen J, Yates PA, McLaughlin T, Friedman GC, O’Leary DD & Barbacid M (1998) Ephrin -A5 (AL1 ⁄ RAGS) is essential for proper retinal axon guidance and topographic mapping in the mammalian visual system ... cleavage sites (B) SDS ⁄ PAGE separation of FN3d–AP purified from conditioned media using anti-placental alkaline phosphatase (PLAP) agarose (C) SDS ⁄ PAGE and silver stain of proteins isolated...

Ngày tải lên: 19/02/2014, 05:20

... resemblance to modal operators Note that no information is added or subtracted by rewriting the feature matrix of Figure as a logical formula These two forms may be regarded as notational variants for ... regarded as a transition graph for a partially specified deterministic finite automaton (DFA) This automaton recognises strings of labels, and has final states which are atoms, as well as final ... m a n y formulas which have an identical interpretation For example, the formulas given in the equation below are satisfied by the same set of a u t o m a t a case : (gen V ace V dat) A case...

Ngày tải lên: 21/02/2014, 20:20

... Scha and Stallard (1988)) The structural semantics stage operates on the parse tree to produce an expression of a language called "EFL" (for English-oriented Formal Language) This language is a ... cases Roughly speaking, these are examples similar to backwards pronominalization cases such as (18) (repeated here as (2 7a) ), in which the potential antecedent is a quantifier or a trace of a ... those that are internal to the current minimal domain and those that are external As each node is processed, a subroutine is called that dispatches on the category of the node and performs any actions...

Ngày tải lên: 21/02/2014, 20:20

... (13-methyl-[1,3]-benzodioxolo[5,6-c]1,3-dioxolo-[4,5-i]-phenanthridinium chloride) (Fig 1), a benzophenanthridine alkaloid derived from the plant Sanguinaria canadensis, has been shown to have antimicrobial, anti-inflammatory, antioxidant, and anticancer ... sanguinarine in immortalized human HaCaT keratinocytes Clin Cancer Res 9, 3176–3182 Adhami VM, Aziz MH, Reagan-Shaw SR, Nihal M, Mukhtar H & Ahmad N (2004) Sanguinarine causes cell cycle blockade and ... cells via bimodal cell death Biochem Pharmacol 63, 1415–1421 Adhami VM, Aziz MH, Mukhtar H & Ahmad N (2003) Activation of prodeath Bcl-2 family proteins and mitochondrial apoptosis pathway by sanguinarine...

Ngày tải lên: 07/03/2014, 12:20

... and drain and the silicon substrate as a back-gate, a transistor configuration can be defined as shown in Fig 5 (a) Transfer characteristics at different temperatures for a constant drain voltage ... surface concentration taking this depth as a reasonable value for substantial tunneling This gives an energy lowering similar to the experimental data as given in Ref [10] Schottky barriers at nanowire ... The values in regime A are found in the range of 0.15 eV, while regime B has a sharp maximum at 0.55 eV and regime C again decreases the activation energy to about 0.25 eV All these values are...

Ngày tải lên: 16/03/2014, 15:14

... text for details), although it was not detected by the immunological assays Psb P Cyanobacteria Glaucophyceae Red algae Diatoms Haptophyceae Brown algae Prasinophyceae Euglenophyceae Green algae ... Cyanobacteria Synechocystis sp PCC6803 Rhodophyceae (red algae) Cyanidioschyzon merolae Nuclear DNA Chloroplast DNA Cyanidium caldarium Chloroplast DNA Bacillariophyceae (diatoms) Thalassiosira pseudonana ... gyrans (B), Laminria japonica (C) and Undaria pinnatifida (D) with antibodies raised against various extrinsic proteins Lane 1, anti-(H-PsbP); lane 2, anti-(H-PsbQ); lane 3, anti-(G-PsbQ); lane...

Ngày tải lên: 23/03/2014, 15:21

... scDNA (B) Ethidium stained agarose gel: lane 1, scDNA only; lane 2, no mAb; lanes 3–6, Bp53-10.1; lanes 7–10, ICA-9 mAb/p53 tetramer molar ratios: lanes and 7, 0.5; lanes and 8, 1.25; lanes and ... supernatants or ascites by means of affinity chromatography using either protein G-Sepharose (Pharmacia) or protein L-Sepharose (Pierce) horseradish peroxidase conjugated anti-rabbit IgG (Sigma) ... lanes and 7, no mAb; lanes and 8, DO-1; lanes and 9, ICA-9; lanes and 10, Bp53-6.1; lanes and 11, Bp53-10.1 Bands denoted as ÔscÕ and ÔocÕ correspond to free monomeric scDNA and open circular...

Ngày tải lên: 30/03/2014, 15:20

... severe forms of anorexia nervosa Upon primary medical stabilization, patients are transferred to a psychiatrically based inpatient eating disorder program further treatment and follow-up Patients and ... the traditional activation cascade using C3 convertases or C5 convertases [13] As a result, C 5a may be generated via thrombin-mediated coagulation abnormalities that have been documented in anorexia ... power of our statistical analysis and make our data vulnerable to a statistical type II error Therefore, our data not allow for advocating complement serum levels as a new biomarker until definitively...

Ngày tải lên: 09/08/2014, 01:21

... 5′-TGA-CTC-TAA-GTG-GCATTC-AAG-G (sense) and 5′-GAT-TCA-GAC-ATC-TCTTCT-CAC-CC (antisense) for IP-10 [24], and 5′-GTG-GGG-CGC-CCC-AGG-CAC-CA (sense) and 5′-CTC-CTT-AAT-GTC-ACG-CAC-GAT-TTC (antisense) for ... significant stimulatory or inhibitory effects were observed (Fig 3) Statistical analysis R76 Data were analyzed on a Power Macintosh computer using a statistical software package (Statview 4.5; Abacus ... contains molecular weight markers (100 base pair [bp] ladder) (b) IP-10 mRNA expression was quantified and normalized to β-actin as the IP-10/β-actin ratio Data are expressed as means ± SEM for...

Ngày tải lên: 09/08/2014, 01:21

... Chicago, IL, USA) Data was analyzed using the Kruskal-Wallis one-way analysis of variance on ranks Pairwise multiple comparison was made with the Holm-Sidak method Foam cell formation and staining ... leukemia virus) reverse transcriptase primed with oligo dT cDNA was amplified with specific primers (48 pmol/reaction) for ABCA1 (forward primer 5'-GAAGTACATCAGAACATGGGC-3' and reverse primer 5'GATCAAAGCCATGGCTGTAG-3' ... Adams K, Adams JM: Indomethacin therapy for patent ductus arteriosus in premature infants: efficacy of a dosing strategy based on a seconddose peak plasma indomethacin level and estimated plasma...

Ngày tải lên: 09/08/2014, 10:20

... Maldonado-Cocco J, Orozco-Alcala J, Prieur AM, Suarez-Almazor ME, Woo P, International League of Associations for Rheumatology: International League of Associations for Rheumatology classification ... participated in its design and coordination, and helped to draft the manuscript All authors read and approved the final manuscript Acknowledgements The authors thank Taschawee Arkachaisri, Daniel ... thermal imaging of the wrist and MCP, normal adult wrists and hands from controls were imaged on separate days Three thermal scans were obtained at each session and the HDI was calculated for each...

Ngày tải lên: 09/08/2014, 10:22

... interpretation of data, manuscript preparation, and statistical analysis SKT participated in acquisition of data, analysis and interpretation of data, and manuscript preparation SC participated in acquisition ... preparation, and statistical analysis J-PP participated in study design, analysis and interpretation of data, and manuscript preparation JM-P participated in study design, analysis and interpretation ... data and manuscript preparation All authors read and approved the final manuscript 13 14 15 16 Acknowledgements The authors thank Virginia Wallis for her assistance in manuscript preparation and...

Ngày tải lên: 09/08/2014, 10:23

... study, ANA staining, saliva collections, data analyses, and manuscript preparation All authors read and approved the final manuscript Proposed genetic predisposition for and fatty acid homeostasis/trans6.NOD-Aec1Aec2 ... O-acyltransferase-1 (SOAT-1) using FCs and free fatty acids (FFAs) ABCA1, ATP-binding cassette, subfamily A [ABC1] member 1; ACAT, acyl-coenzyme A: cholesterol acyltransferase; ApoE, apolipoprotein ... primarily the pathophysiological and biochemical abnormalities that subsequently result in the activation of the autoimmune attack against the submandibular and lacrimal glands [10], is a single...

Ngày tải lên: 09/08/2014, 13:22

... chi-square and one-way analysis of variance (ANOVA) were performed to evaluate group differences Page of across demographic and medical variables, as well as markers of ANS dsyregulation Any variables ... used as a marker of increased vagal activity and has been consistently associated with greater capacities to regulate stress, emotional arousal, and attention [22] while low HRV has been associated ... this article as: Dao et al., Autonomic cardiovascular dysregulation as a potential mechanism underlying depression and coronary artery bypass grafting surgery outcomes Journal of Cardiothoracic...

Ngày tải lên: 10/08/2014, 09:22

... hyperplasia Pediatr Dev Pathol 2004, 7:370-379 Sacktor N, Haughey N, Cutler R, Tamara A, Turchan J, Pardo C, Vargas D, Nath A: Novel markers of oxidative stress in actively progressive HIV dementia ... cytokines and amelioration by antioxidants Cardiovasc Toxicol 2004, 4:287-302 Gomez-Vera J, de Alarcon A, Jimenez-Mejias ME, Acosta D, Prados D, Viciana P: Hyperglycemia associated with protease inhibitors ... Pradhan L, Ali M, Agrawal KC: HAART drugs induce oxidative stress in human endothelial cells and increase endothelial recruitment of mononuclear cells: exacerbation by inflammatory cytokines and...

Ngày tải lên: 12/08/2014, 04:20

... years Potential confounders of age, gender, BMI, and tibial plateau area for annual change in cartilage volume were included in multivariate analyses A P value less than 0.05 (two-tailed) was regarded ... Medial compartment Annual change in cartilage volume Cartilage defects progress vs no change Lateral compartment Annual change in cartilage volume Cartilage defects progress vs no change Annual ... coefficients of variation for the medial and lateral tibial cartilage volume measures were 3.4% and 2.0% respectively [35,36] Annual change in cartilage volume was calculated as follow up cartilage volume...

Ngày tải lên: 12/08/2014, 11:22

... plasmid pcDNA3.1HHR2 3A by PCR The 5' primer used was 5'ATCCAAGACGGAATTCACGCCGCAGGAGAAAGAAGCTATAG-3'; the 3' primer for the APOPEC3G-UBA2 fusion was 5'-ATCGTACTCGAAGCTTCTAACTCAGGAGGAAGTTGGCAG-3'; ... the E plasmid was 5'-ATCCAAGACGGAATTCCTAGAACTCGTTTTCCTGATTCTGGAG-3' and the 3' primer used for the U and M plasmid was 5'-ATCCAAGACGGAATTCGTTTTCCTGATTCTGGAG-3' The UBA2 gene fragment was amplified ... essential for Vif function J Biol Chem 2005, 280(19):18573-18578 Shirakawa K, Takaori-Kondo A, Kobayashi M, Tomonaga M, Izumi T, Fukunaga K, Sasada A, Abudu A, Miyauchi Y, Akari H, Iwai K, Uchiyama...

Ngày tải lên: 13/08/2014, 05:21

... formalin fixed, paraffin embedded tissues or studied by IHC using the anti-p30 antibody, anti-CD3 for Tcell lineage identification (DAKO Corporation, Carpinteria, CA Catalog # A4 52), and anti-PAX5 ... anti-PAX5 for B-cell lineage (Goat anti-Pax 5, Santa Cruz Biotechnology, Santa Cruz, CA, Catalog #sc-1974) [14] Criteria for histopathological diagnosis were as described [15] As shown in Table 1, ... pneumonia virus of mice (PVM) in a mouse macrophage cell line Virol Journal 2007, 4:48-51 Shin J-J, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang J-I, Rebres R, Roach T, Seaman W, Simon MI, Fraser...

Ngày tải lên: 13/08/2014, 06:20