... mortalities on a log concentration/% mortality sheet The procedure for estimation of the LC50 is as follows: Indicate the concentrations or dilutions used in the dilution series on the Y-axis Plot the ... Ia is the percent inhibition of concentration a, Ac is the area of the control growth curve, and Aa is the area of the growth curve of concentration a The concentration – effect curves and the ... percent mortality on the horizontal line at the height of each concentration or dilution Connect the plotted mortality points on the graph with a straight line Locate the two points on the graph that...
Ngày tải lên: 11/08/2014, 17:22
... result of the reaction between dye and hydroxyl radicals The dye removal was investigated as a function of FeOOH concentration Under the assumption that OH radical is the only reactive species on dye ... reaction with the H2O2 and FeOOH concentration The rate of dye removal under the presence of H2O2 was not proportional to the FeOOH concentration This is because the FeOOH surface not only generates ... concentration In contrast with the result of Figure 2, dye removal was not proportional to the FeOOH concentration Rather, the observed first order rate constant for dye removal was reduced in the presence...
Ngày tải lên: 05/09/2013, 08:40
Báo cáo khoa học: Production and utilization of hydrogen peroxide associated with melanogenesis and tyrosinase-mediated oxidations of DOPA and dopamine docx
... derived only from the oxidation of any H2O2 formed, and that these responses were proportional to the concentration of the reactive molecule Quantitative determinations were made following the establishment ... tyrosinase-mediated reactions Fig Electrochemical responses resulting from H2O2 production during the autoxidation of dopamine following the addition of varying amounts of the diphenol into solutions of NaCl ... diminished in the presence of catalase, indicating that the H2O2 generated during these reactions was utilized as a cofactor in generating the corresponding o-quinones Additionally, inhibition studies...
Ngày tải lên: 07/03/2014, 17:20
Báo cáo khoa học: Glucose oxidase prevents programmed cell death of the silkworm anterior silk gland through hydrogen peroxide production ppt
... peroxide concentration throughout the incubation period Combining these observations, we concluded that the production of hydrogen peroxide by the enzymatic reaction of GOD results in the inhibition ... cocoons, and the first step of cocooning is the construction of a scaffold with silk thread to keep the larvae in a position suitable for completing the cocoon The silk scaffold remains as the ... formation In addition, the suggested natural functions of GOD include the detoxification of noxious plant components involved in plant defense responses [7] In contrast, the in vivo function of...
Ngày tải lên: 15/03/2014, 00:20
Effect of hydrogen peroxide on the destruction of organic contaminants synergism and inhibition in a con
... electrons in the conduction band by oxygen reduction is the rate-controlling step in the photocatalytic mechanism [26,54] Consequently, conditions that favor the removal of conduction band electrons can ... function of the magnitude of hydrogen peroxide concentration relative to other reaction conditions (i.e., contaminant concentration, total organic carbon, UV light flux) When such conditions are ... solution (type and concentration of organics, pH, presence of inorganic ions), the concentration of oxygen in the reaction solution, the magnitude of the intensity and wavelength of the UV light, the...
Ngày tải lên: 16/03/2014, 00:07
Báo cáo khoa học: Mammalian 105 kDa heat shock family proteins suppress hydrogen peroxide-induced apoptosis through a p38 MAPK-dependent mitochondrial pathway in HeLa cells potx
... addition, when the cells were fractionated into a soluble fraction and a particulate fraction containing mitochondria by treatment with digitonin, a large amount of cytochrome c was detected in the ... fraction concomitant with its decrease in the particulate fraction The release of cytochrome c from mitochondria was suppressed by the overexpression of Hsp105a or Hsp105b Several studies have demonstrated ... the mitochondria, and leads to the release of cytochrome c and other proteins from the mitochondrial intermembrane space [28,29] However, we did not detect Bax translocation to the mitochondria...
Ngày tải lên: 16/03/2014, 06:20
Báo cáo khoa học: An inserted loop region of stromal ascorbate peroxidase is involved in its hydrogen peroxide-mediated inactivation pot
... when ascorbate is absent [16] The loop structure may therefore influence the location of the radical through interaction with the cation and thus affect the reaction of radicals to excess hydrogen ... Poulos TL & Dawson JH (1999) A study of the K+-site mutant of ascorbate peroxidase: mutations of protein residues on the proximal side of the heme cause changes in iron ligation on the distal side ... mm)1Æcm)1) The concentration of hydrogen peroxide was determined from the absorption at 240 nm (e ¼ 0.0394 mm)1Æcm)1) Preparation of ascorbate- and oxygen-free APX solution APX in the elution buffer...
Ngày tải lên: 16/03/2014, 14:20
Synthesis of silicon nanowires after hydrogen radical treatment
... reveal the high relative intensity of the (222) peak compared with the other peaks On the other hand, hydrogen radical treatment was performed on the substrate for at 400 °C to fabricate the metal ... films After the fabrication of the indium nanocrystal catalysts, the SiNWs were synthesized using the hydrogen radical-assisted reaction deposition method for h, at the same temperature The morphologies ... in the inset of Fig The set of diffraction spots visible in the SAED pattern reveal that this particular SiNW contains a twin Moreover, it can be indexed as the diffraction along the [110] zone...
Ngày tải lên: 16/03/2014, 15:13
Báo cáo khóa học: Mechanistic insight into the peroxidase catalyzed nitration of tyrosine derivatives by nitrite and hydrogen peroxide doc
... kinetics were then studied as a function of both the phenol and nitrite concentrations For each substrate, the conditions required to study the rate dependence on the reactants concentrations were ... solution [12] Nonetheless, if the active species does not diffuse into the solution, but reacts with the substrate bound close to the active site, nitration by these species could occur before their ... nitration of on nitrite concentration in 200 mM phosphate buer (pH 7.5), at 25 C The inset shows an expansion of the plot in the low nitrite concentration range Formation of phenol dimers by the...
Ngày tải lên: 16/03/2014, 16:20
Báo cáo khoa học: Microperoxidase 8 catalysed nitrogen oxides formation from oxidation of N-hydroxyguanidines by hydrogen peroxide ppt
... formation of the iminoxy-radical and one can propose the mechanism shown in Scheme for the oxidation of N-hydroxyguanidine by H2O2 in the presence of MP8 Further one-electron oxidations of the intermediate ... derivatization under the same conditions also resulted in the detection of citrulline (data not shown) These results indicated that the low stability of CN-orn under those conditions prevents ... through monoelectronic transfers, Fe(CN)6K3, also led to the selective formation of cyanamide (data not shown) This constitutes another argument in favour of a monoelectronic process in the reactions...
Ngày tải lên: 17/03/2014, 09:20
Báo cáo khoa học: Hydrogen peroxide efflux from muscle mitochondria underestimates matrix superoxide production – a correction using glutathione depletion pptx
... the GSH concentration was determined as the increase in fluorescence (excitation 390 nm, emission 478 nm) after the addition of UÆmL)1 glutathione S-transferase and incubation at 20–25 °C in the ... rotenone + AA uncorrected), supporting the contention that CDNB pretreatment corrects for most of the effects of these matrix components in intact mitochondria Effect of monochlorobimane (MCB) on ... technique measures contributions from both the mitochondrial NADH and NADPH, the content of NAD+ plus NADH in skeletal mitochondria is much greater than the combined NADP+ and NADPH [31] Moreover, the...
Ngày tải lên: 22/03/2014, 21:21
Re-Evaluation of Some Organic Chemicals, Hydrazine and Hydrogen Peroxide potx
... in the United States, one in the United Kingdom and one in the Netherlands) were carried out and these now provide the most relevant, informative data on which to base an evaluation All of the ... in the second highest decile On balance and given the largely unsupportive findings from the other studies, the evidence from this one study was not considered to be sufficiently strong to conclude ... comparison with national rates, the Dutch study did the same, but also compared the exposed with a different unexposed cohort One of the studies from the United States compared the exposed with national...
Ngày tải lên: 28/03/2014, 19:20
RE-EVALUATION OF SOME ORGANIC CHEMICALS, HYDRAZINE AND HYDROGEN PEROXIDE ppt
... about the evidence for or against carcinogenicity provided by the available data These evaluations represent only one part of the body of information on which regulatory measures may be based Other ... than one of them Thus, for example, the action of an agent on the expression of relevant genes could be summarized under both the first and second dimensions, even if it were known with reasonable ... describing the rationale for this conclusion is added to the evaluation narrative; an additional evaluation may be made for this broader group of compounds if the strength of the evidence warrants it The...
Ngày tải lên: 28/03/2014, 19:20
Báo cáo khoa học: Enhanced sensitivity to hydrogen peroxide-induced apoptosis in Evi1 transformed Rat1 fibroblasts due to repression of carbonic anhydrase III docx
... transfected into Rat1 cells at the same concentration (Fig 7A,B, Non sp siRNA) The effect of caIII siRNA1 on H2O2 sensitivity in transfected Rat1 cells was then investigated by monitoring caspase catalytic ... either a nonspecific siRNA (Non sp siRNA) or a caIII-specific siRNA (siRNA 10 nM) The columns are the mean of an experiment performed in quadruplicate and the error bars the standard deviation ... reduction in caIII gene transcripts and protein Together, these results confirm that Evi1-mediated stim- ulation of H2O2-induced cell death is due to the reduction in cellular levels of the caIII...
Ngày tải lên: 29/03/2014, 08:20
Báo cáo hóa học: " Involvement of intracellular free Ca2+ in enhanced release of herpes simplex virus by hydrogen peroxide" ppt
... the untreated control was observed in the cell-free fraction, but no increase was observed in the cell-associated fraction The proportions of cell-free virus in the total amount of virus in the ... electron microscope Competing interests The author(s) declare that they have no competing interests Authors' contributions EA and YY conceived of the study, analyzed the results and wrote the manuscript ... Ca2+ Thereafter, treatment with mM H2O2 for h was initiated In this condition, the amount of cell-free virus was 150% of that in the untreated control, whereas it was increased to 450% of the control...
Ngày tải lên: 20/06/2014, 01:20
Báo cáo nghiên cứu khoa học: " EXPERIMENTAL AND THEORETICAL ANALYSIS OF A CRITICAL CHEMICAL REACTION: DECOMPOSITION OF HYDROGEN PEROXIDE (H2O2)" potx
... to give the concentrations in table The pH of the solution was adjusted with the addition of nitric acid, from Fluka (65 vol.%) The solution of Iron(III) Nitrate was made with the Iron(III)-nitrate ... of pH and catalyst concentration to the kinetics of the reaction The values of the optimized reaction rate constant, k, and the values of the objective function, OF [equation (13)], for all experiments ... published, the concentrations were not as high as the ones used in this study Most of the works in the literature focused on the use of hydrogen peroxide for the remediation of wastewater and the concentrations...
Ngày tải lên: 22/07/2014, 03:20
KHẢO SÁT SIÊU ÂM LÒNG HẬU MÔN VỚI HYDROGEN PEROXIDE TRONG BỆNH RÒ HẬU MÔN VÀ RÒ HẬU MÔN - ÂM ĐẠO doc
... fistulas The endoanal ultrasonography with hydrogen peroxide is able to establish the relation between the fistulas and the sphincter muscles, to classify the fistulas and to identify the inner ... were performed by the endoanal ultrasonography with the 360 rotating tranducer and BK equipment (BK Hawk 2102 – EXL) Radiologists used the 3D software (BK3Di Type 6503) to reconstruct images Findings ... Findings in operations were the gold standards Results: in 132 patients (109 males, 23 females, mean age 49.67), there were a variety of anal and ano-vaginal fistulas The endoanal sonography was able...
Ngày tải lên: 01/08/2014, 16:20
Báo cáo y học: "HIV-1 Nef increases astrocyte sensitivity towards exogenous hydrogen peroxide" potx
... described in the Material section The times indicated are relative to the moment of adding AlamarBlue® reagent to the cell culture medium The relative fluorescence represents the ratio of the fluorescence ... medium containing 10% FCS for a further 48 h The medium was then exchanged and cell viability assay was performed as described in the Methods section The relative fluorescence represents the ratio ... Authors’ contributions MHL conceived and designed the experiments, SM performed the experiments, SM and MHL analyzed the data, MHL wrote the paper All authors have read and approved the final...
Ngày tải lên: 11/08/2014, 21:21
Báo cáo khoa học: " Evaluation of LHP® (1% hydrogen peroxide) cream versus petrolatum and untreated controls in open wounds in healthy horses: a randomized, blinded control study" pdf
... was used for sedation and analgesia Two × cm areas were shaved at standardized locations on each side of the neck (one on the left and one on the right side) and a third area of the same size was ... evaluate the effect of the two different treatments on wounds at identical anatomical locations Also the treated wounds were on the opposite side of the neck to prevent interaction between the different ... approximately cm cranial to the previously described location Five horses had the cranial wound randomly chosen on the left side and five horses had it on the right side The wound locations were assigned...
Ngày tải lên: 12/08/2014, 18:22
Báo cáo y học: "Aspergillus fumigatus allergen expression is coordinately regulated in response to hydrogen peroxide and cyclic AMp" doc
... growth on hydrogen peroxide Only one allergen gene, Asp f was repressed under this condition Thus, the expression of 14/17 allergen genes was induced under a single condition suggesting the possibility ... allergen expression levels in Page of 11 response to transfer to media containing these components or growth under these conditions Samples were analysed at h, h, h and 24 h but only the h time point ... because little variation in expression was observed between the time points analysed None of the conditions tested resulted in coordinated high levels of expression or repression of the allergen genes...
Ngày tải lên: 13/08/2014, 13:22