activation of adhesion and cell polarisation

... that ligand-independent activation of Met and EGFR in A431 cells relies on cell adhesion to a substratum rather than cell- cell adhesions We reasoned such adhesion- induced activation of Met and EGFR ... determinant for Met and EGFR activation in A431 cells Adhesion- induced activation of Met and EGFR in A431 cells is independent of Src, ROS, or cytoskeleton Adhesion- induced activation of RTKs has been ... of cell adhesion [7, 8] Likewise, it was reported that the ligand-independent activation of Met relies on cell adhesion to fibronectin via α5β1 integrins [9] However, the mechanism how cell adhesion...

Ngày tải lên: 10/08/2014, 10:20

... [14,15] and adhesion of polymorphonuclear cells and monocytes to CNS cells [16,17] Schwann cells (SCs) are glia cells found in the PNS In addition to their roles in myelination, trophic support and ... the presence of cytokines Natural ligands of ICAM- 1and LFA-1 are expressed on the surface of T and B lymphocytes, natural killer cells, monocytes, macrophages and granulocytes [33], and interaction ... immunocompetent cells after exposure to foreign antigen and migration of primed T cells into the peripheral nerve, ICAM-1 ⁄ LFA-1 adhesion molecules act on both the induction and effect phases of the...

Ngày tải lên: 18/02/2014, 18:20

... differentiation effect of pregnancy Y E Liu et al Fig Induction of Jak and Stat5 activation and b-casein expression by DPA and EPA (A) MCF-10 cells Cells were treated with 10 lM of DHA, DPA, and EPA for ... favor of n-3 PUFA and particularly an n-3 DPA and EPA An increase in n-3 DPA and EPA, and perhaps other n-3 PUFAs, stimulates Jak2 and Stat5 activation, and induces b-casein expression and gland ... induction of mammary differentiation Using MCF-10 mammary epithelial cells, we analyzed the effect of DPA, EPA, and DHA on activation of Jak2 and Stat5 Whereas DPA and EPA activated Jak2 and Stat5,...

Ngày tải lên: 16/03/2014, 10:20

... within the G1phase of the cell cycle [4] pRb sequesters members of the E2F gene family of transcription factors Cell cycledependent phosphorylation of pRb by CDKs inactivates pRb and inhibits pRb ... associated with increased presence and activation of glial cells Using post mortem brain tissue we have investigated whether activation/ occurrence of microglia and astrocytes in AD brain can be ... microglia and astrocytes with the Braak score for neurofibrillary changes and high levels of activated glial cells in cases with Braak score B and C (figure 1) We observed maximal neuronal ppRb and...

Ngày tải lên: 19/06/2014, 22:20

... upregulation of BMP-2 mRNA after injury Several factors can determine activation of BMP signaling independently of the expression of one ligand, including secretion and solubility of the ligand(s), ... presence of secreted or intracellular inhibitors and receptor regulation [35] Our data showing phosphorylation of SMAD-1/-5 suggest activation of BMP signaling Activation cal injury of the WNT/β ... corresponding squared areas (h) A graphic summary of the proportion of phospho-SMAD-1/-5-positive cells and the expression of BMP-2, FRZB, metalloproteinase (MMP)-3 and MMP-13 mRNAs in this experiment with...

Ngày tải lên: 09/08/2014, 08:22

... the activation process of these cells, namely IFN-g and TNF-a Results from IFN-g and TNF-a are shown in Figures and 2, respectively As to IFN-g assays (Figure 1), non-activated monocytes of control ... against Candida albicans Monocytes obtained from peripheral blood of control subjects and patients, before and after activation with different concentrations of interferon-gamma (IFN-g), and after ... monocytes/macrophages with a consequent increase of apoptosis, expression of adhesion molecules of the endothelium and synthesis of other pro-inflammatory cytokines such as IL-1 and IL-6 Similar to results regarding...

Ngày tải lên: 11/08/2014, 03:20

... authors thank Professors William Brett and Jim Hughes of the Department of Life sciences and Tista Page of 11 (page number not for citation purposes) Journal of Immune Based Therapies and Vaccines ... Induction of anti-tumor effector T cells Since induction of cytolytic effector cells is pivotal in ensuring the success of tumor vaccines, we investigated relative efficacy of phytols and known ... (Fig 4A and 4B), but there was no sign of splenomegaly with 40–80 μg of PHIS-01 (Fig 4B) Average spleen weights and cell numbers for each group of mice were reported in Table Induction of lupus-autoantibodies...

Ngày tải lên: 11/08/2014, 10:23

Ngày tải lên: 11/08/2014, 10:23

... via activation of cyclooxygenase (COX) and release of the bronchodilator prostaglandin E2 (PGE 2) [21] The mechanism behind AHR to kinins appears to involve activation of intracellular MAPKs and ... cAMP and JNK pathway cAMP has the ability to inhibit JNK activation in human airway smooth muscle cells [54], and in rat renal mesangial cells Forskolin inhibits MAPK [55] Activation of ERK5 and ... oxygen species production, cell adhesion molecule expression and the release of cytokines from activated T-helper cells, airway epithelial cells, basophils, monocytes and macrophages [52] The mechanisms...

Ngày tải lên: 12/08/2014, 11:20

... tumorigenesis of brain tumor (Nf2, Stat2), tumorigenesis of polyp (Asph, Smad4), aggregatability of colon cancer cell lines (Cd82), cell spreading of colon cancer cell lines (Smad4), contact inhibition of ... formation of aberrant crypt foci (NR5A2, PTGER4), cell death of enteroendocrine cells (GCG, PYY), growth of crypt cells (NKX2, NKX3) 16 221 Global Cardiovascular system development and function, cellular ... and morphology of melanoma cells (HSPE, LSP1), adhesion of lymphoma cells (HSPE) 20 91 Adult colon Up (D); down (CRC); up (A/M/S/T) Cell death, hematological disease, immunological disease, cell- to-cell...

Ngày tải lên: 14/08/2014, 07:22

... mutants of each of Rac1, Cdc42 and RhoA to assess the involvement of Rho family members in LPA-induced PAK1 activation N17Rac1- or N17Cdc42-transfected cells showed reduced activation of PAK1 ... PAK and FAK [53], involvement of PIX, Nck and GIT in LPA-induced FAK phosphorylation and cell motility is under current investigation Following G protein activation by LPA, one of the intracellular ... phosphorylation and cell motility Therefore, we undertook to investigate the roles of PAK1 activation in LPA-induced cell motility and present herein strong evidence that PAK1 activation is involved...

Ngày tải lên: 30/03/2014, 13:20

... potency of ATP and ADP in the inhibition of LXF-289 cell proliferation Cell cycle analysis revealed that inhibition of proliferation of LXF-289 cells by ATP and ADP was mediated by retardation of cell ... Fig Effect of ATP and ADP on cell cycle progression of LXF-289 cells (A) Accumulation of LXF-289 cells in the S phase LXF-289 cells were incubated with ATP or ADP (10–100 lM) for 24 h, and the distribution ... Attenuation of LXF-289 cell proliferation by ATP is mediated by the activation of the MEK ⁄ ERK1 ⁄ 2, PI3K and p38 MAPK pathways Activation and translocation of the transcription factors NF-jB1 (p50) and...

Ngày tải lên: 07/03/2014, 12:20

... endocrine cells FEBS Journal 272 (2005) 2746–2759 ª 2005 FEBS L Chen et al Activation of Cdx-2 expression by cAMP Fig Comparison of the effect of H-89 on expression of the proglucagon and Cdx-2 ... factors and signaling molecules that regulate the expression of this promoter We found that cotransfection of the Cdx-2 cDNA led to upregulation of the expression of the Cdx-2 gene promoter in cell ... expression of proglucagon gene mRNA and its promoter in this cell line, in contrast to significant activation by forskolin ⁄ IBMX in primary pancreatic islet cell cultures or in intestinal endocrine L cell...

Ngày tải lên: 30/03/2014, 16:20

... address this issue of PtdIns3K activation and its relationship to Erk activation in a broad range of cell types Activation of PKB ⁄ akt has been shown to depend upon the production of 3-phosphorylated ... B cells and a B -cell line, full activation of Erks was dependent upon the activation of PtdIns3K Several years ago, our group showed, by use of two different PtdIns3K inhibitors, LY294002 and ... required for Erk activation A B Both wortmannin and LY294002 not affect the activation of Erks in TF-1 cells Next we investigated the relationship of PtdIns3K activation and Erk activation in a...

Ngày tải lên: 30/03/2014, 20:20

... OVCA429 cell adhesion (A) ALK3QD expression increases trypsin-sensitivity of OVCA429 cells Subconfluent monolayer cultures of 429T-ALK3QD cells (clones A44 and A54), and 429T control cells, were ... analysis, cell motility, cell adhesion and spheroid formation assays, and drafted the manuscript MM performed cell motility and invasion assays MN participated in the study design and coordination and ... actively growing primary cultures of OSE and EOC cells, and the EOC cell lines CaOV3, SkOV3, and OVCA429, we readily detected expression of the type I BMP receptor ALK3 and the type II receptor BMPR2...

Ngày tải lên: 20/06/2014, 07:20

... laminin, and mediates cell- cell and cell- matrix adhesion [12,13] The present study aimed to determine the impact of a1, 2-FT gene transfection on the expression of CD44 on cells and the effects of ... groups (P > 0.05) The mRNA levels of CD44 and a1,2-FT in RMG-I and RMG-I-H cells The 2-ΔΔCT value of mRNA level of CD44 in RMG-I-H cells is 79% of that in RMG-I cells, which had no significant ... level of a1,2-FT in RMG-I-H cells was increased by 3.07 times of that in RMG-I cells detected by Real-time PCR (P < 0.01) (Figure 4) HA-mediated cell adhesion and spreading The adhesion of RMG-I-H...

Ngày tải lên: 10/08/2014, 10:21

... chemokines and cell adhesion molecule in A549 cells cocultured with MT-2 cells Induction Induction of expression of cytokines, chemokines and cell adhesion molecule in A549 cells cocultured with MT2 cells ... http://www.retrovirology.com/content/5/1/86 Figure Secretion of IL-8 and CCL20 and Induction of cell surface ICAM-1 expression in A549 cells cocultured with MT-2 cells Secretion of IL-8 and CCL20 and Induction of cell surface ICAM-1 ... the production of cytokines and chemokines and induced the surface expression of cell adhesion molecule http://www.retrovirology.com/content/5/1/86 Activation of NF-κB and AP-1, and viral promoter...

Ngày tải lên: 13/08/2014, 05:21

... http://ccforum.com/content/14/6/R201 Page of 13 Figure Interdependent effects of shear stress and cell activation on PMN adhesion Adhesion of neutrophils under different activation protocols (mean ± SEM; ... endothelium activation of PMN decreased adhesion in comparison to non-activated PMN ((b and e), P < 0.01, ANCOVA) On sub-maximal activated endothelium (c and f), activation of PMN also decreased adhesion ... contributions AP and BN conceived of the study, participated in its design and coordination, and drafted the manuscript VS and AH carried out the adhesion assays JR and HAH participated in the design of the...

Ngày tải lên: 13/08/2014, 21:21

... at cell- adhesions As cell adhesions play a pivotal role in the proper function of cells, misregulation of cell adhesions often leads to severe pathological consequences Altered cell- cell and cell- matrix ... essential for the formation and functioning of tissues Cell- cell adhesion is one of the corner stones in the arising of multicellular organisms Depending on the context, cell- cell adhesion could serve ... integrity of tissues, signal transduction across cells, cellular recognition and triggering of immune responses Tissue organizations not only depend on cell- cell adhesions In epithelium and muscles, cells...

Ngày tải lên: 09/09/2015, 08:13

... Structures and Functions of Cell As the basic unit of life, the cell is a biologically complex system [49] There are two types of cells: prokaryotic and eukaryotic Prokaryotic cells, such as archaea and ... components of the nuclear lamina and sarcomeres They also participate in cell- cell and cell- matrix adhesive junctions 2.2 Basics of Cell Adhesion In biological systems, cell adhesion is an integrated ... migration, and cell- cell and cell- ECM adhesions In the case of adhesion, the lateral mobility of receptors determines the speed at which receptors move towards their corresponding ligands, the...

Ngày tải lên: 10/09/2015, 15:48