activation induced cell death and cytokine production

... Daniels MA (2009) ERK and cell death: ERK location and T cell selection FEBS J Martin P & Pognonec P (2009) ERK and cell death: cadmium toxicity, sustained ERK activation and cell death FEBS J Taylor ... Journal compilation ª 2009 FEBS S Cagnol and J.-C Chambard ERK and cell death Table Continued Stimuli inducing cell death Duration of ERK activation promoting cell death Human melanoma C8161,WM164 Mel ... in activation of the extrinsic pathway by death receptor ligands such as TNF-related apoptosisinducing ligand (TRAIL) [34,42–46], TNFa [47–49], Fas [50,51] or CD40 ligand [52,53] Cell death induced...

Ngày tải lên: 29/03/2014, 08:20

... Cellular interaction between human lymphoid cells and RA-SNC77 cells (a) Adhesion between lymphoid cell lines and RA-SNC77 Biscarboxyethyl carboxyfluorescein-labeled lymphoid cells (2 × 105 cells/well) ... 7500 Cytokine production from RA-SNC77 cells cocultured with lymphoid cell lines RA-SNC77 cells (2 × 104 cells/well) were cultured for days in a 12-well culture plate Lymphoid cells (1 × 106 cells/well) ... Lymphoid cells (1 × 106 cells/well) were plated onto a monolayer of RA-SNC77 cells and incubated for hours The lymphoid cells bound to the surface of the RA-SNC77 cells were removed, and the interaction...

Ngày tải lên: 09/08/2014, 06:22

... [12], and induce death in various cell types [14,15] Oxidative stress -induced cell death, both apoptosis and necrosis, can involve a number of cellular mechanisms, one of which includes the activation ... ∆ΨM CELL DEATH ∆ metabolic activity Figure Proposed mechanism of QD induced cell death involving Fas, lipid peroxidation and mitochondrial impairment Proposed mechanism of QD induced cell death ... Fas(CD95)/FasL interactions required for programmed cell death after T -cell activation Nature 1995, 373(6513):444-448 Thorburn A: Death receptor -induced cell killing Cell Signal 2004, 16(2):139-144 Wallach...

Ngày tải lên: 11/08/2014, 00:22

... evaluated before and after incubation with two cytokines involved in the activation process of these cells, namely IFN-g and TNF-a Results from IFN-g and TNF-a are shown in Figures and 2, respectively ... endothelium and synthesis of other pro-inflammatory cytokines such as IL-1 and IL-6 Similar to results regarding pro-inflammatory cytokines, high levels of anti-inflammatory cytokines (IL-10 and TGF-b) ... inhibiting intracellular reactive oxygen species rise, proteasome activation, and subsequent NFkB activation in smooth muscle cells The FASEB Journal 2003, 17:743-745 Ares MPS: Atherosclerosis: cell biology...

Ngày tải lên: 11/08/2014, 03:20

... correlations between CD4+ T -cell counts and DC subset counts or cytokine production (A) CD4+ T -cell counts and mDC counts; (B) CD4+ T -cell counts and pDC counts; (C) CD4+ T -cell counts and IL-12 concentration; ... load and DC subset counts or cytokine production (A) plasma viral load and mDC counts; (B) plasma viral load and pDC counts; (C) plasma viral load and IL-12 concentration; (D) plasma viral load and ... from 6.8 cells/μl (range, 2.1 to 12.2 cells/μl) to 11.1 cells/μl (range, 1.6 to 27.2 cells/μl) but sharply decreased to the nadir on day 15 p.i (mean, 3.8 cells/μl; range, 1.2 to 6.4 cells/μl)...

Ngày tải lên: 13/08/2014, 01:20

... untreated control cells; lane 4, Glu-Pg-treated cells; lane 5, Glu-Pg- and mAb A10.2-treated cells; lane 6, Glu-Pg- and mAb 34D3-treated cells Bands in lanes and correspond to forms I and II of plasmin(ogen) ... their respective contribution to Gluand Lys-Pg binding and activation onto fibrin and cells, as well as their effects on fibrinolysis and on plasmin -induced cell apoptosis The LBS specificity of ... Ho-Tin-Noe et al Plasminogen transitions and proteolysis A Fig Anti-LBS A10.2 and 34D3 mAbs protect cells from plasminogen activation- induced cell detachment CHO-K1 cells were incubated with Glu-Pg at...

Ngày tải lên: 20/02/2014, 01:20

... both whole cells and isolated mitochondria and fatty acid amount and composition were measured When grown on a standard medium, yeast cells only contain monounsaturated fatty acids and not polyunsaturated ... Kinetics of Bax -induced cell death varied accordingly to the extent of Bax -induced mitochondrial lipid oxidation In the minimal medium without dioctylphtalate, Bax -induced cell death kinetics ... Erythroleukemia and Neuroblastoma cells Biochem Biophys Res Commun 272, 345–350 17 Maccarrone, M., Melino, G & Finazzi-Agro, A (2001) Lipoxygenases and their involvement in programmed cell death .Cell Death...

Ngày tải lên: 23/03/2014, 21:20

... expression augments H pyloriinduced cell death and DNA fragmentation in gastric epithelial cells To investigate the relations of PAR-2 expression, cell death, and DNA fragmentation, cells were transfected ... increased cell death and DNA fragmentation in H pylori-infected AGS cells These results demonstrate that the expression and the activation of PAR-2 induced by H pylori may prevent apoptotic cell death ... the cells without transfection and cultured in the presence of H pylori Figure Inhibition of PAR-2 expression augments H pyloriinduced cell death and DNA fragmentation in AGS cells (A) AGS cells...

Ngày tải lên: 18/06/2014, 16:20

... expression and androgen production in theca cells via activation of the PI3K pathway Both the PI3K and the MAPK pathways coordinately regulate androgen production in bovine theca cells Methods ... LH -induced Akt phosphorylation, CYP17A1 expression, and androgen production in theca cells These results suggest that LH stimulates CYP17A1 mRNA expression and androgen production in theca cells ... Figure bovine theca cells Effects of PI3K inhibitors on androstenedione production in Effects of PI3K inhibitors on androstenedione production in bovine theca cells Bovine theca cells were stimulated...

Ngày tải lên: 20/06/2014, 07:20

... both monocytes and CD4? T cells against cell death (p = 0.0001 and p = 0.0481, respectively), and implicates ROS formation Fig Kinetics and magnitude of ROS production in T cell and monocyte populations ... immune cell subsets a PBMC were treated with varying concentrations (0, 0.5, 1, 2.5, 5, and 10 mM) of 8-nm ZnO NPs for 24 h and viability of CD3? T cells, CD4? T cells, B cells, NK cells, and monocytes ... ability, and inherent cellular capacities for ROS production Monocytic cells displayed the greatest susceptibility and intracellular ROS production following NP exposure, followed by NK cells,...

Ngày tải lên: 22/06/2014, 00:20

... vacuolation and cell death induced by MG-132 76 Vacuoles induced by proteasome inhibition contain ER 78 Caspase activation is not required for MG-132 -induced cell death or ER ... bortezomib to induce cell death by driving c-MYC expression, which in turn upregulates ATF4 and CHOP to drive apoptosis 3.2 Bortezomib induced cell death is reduced by rapamycin and by inhibition ... Bortezomib induced cell death is reduced by rapamycin and by inhibition of the unfolded protein response 37 Early UPR markers are induced by bortezomib but unaffected by rapamycin in Elt3 cells...

Ngày tải lên: 24/08/2014, 09:41

... play a role in mitotic cell death induced by other means 3.4.1 MG132 induced mitotic cell death 3.4.1.1 p73 is required for MG132 mitotic cell death 3.4.2 Mitotic cell induced by plk1 silencing ... 2.2.3.1 Cell death assays 2.2.3.2 Cell cycle and phospho-histone-H3 analysis 2.2.4 Immunofluorescence and confocal microscopy 2.2.5 Protein isolation and analysis 2.2.5.1 Total cell lysate 2.2.5.2 Cell ... mediated cell death 146 Chapter 153 Discussion 149 150 150 151 5.1 Overview 5.2 Regulation of mitotic cell death by p73 5.2.1 p73-dependent mitotic cell death require spindle checkpoint activation and...

Ngày tải lên: 13/09/2015, 21:21

... T- and B -cell development T cell activation is responsive to IL-2 CREB undergoes rapid phosphorylation on Ser119 upon IL-2 stimulation and facilitates AP-1 activation and subsequent IL-2 production, ... 1.1 Programmed cell death Programmed cell death (PCD) is an evolutionary conserved process that is important for multicellular organisms to delete unwanted cells during development and homeostasis ... leading to caspase activation and cell death Death signals originating from the death receptors (TNFR or Fas/CD95/Apo1) or resulting in mitochondrial dysfunction trigger the activation of caspase-8...

Ngày tải lên: 16/09/2015, 15:54

... bacterial biology and understanding mechanisms of cell survival and cell death seems more fitting now, than ever 1.1 Cellular environment induced death Reactive oxygen species mediated cell death Aerobic ... (0.1mM) and thiourea (60µM) were added to cells minutes prior to addition of 20mM H2O2 Thiourea, as indicated by cell survival and histogram prevented radical formation and cell death Neither cell death ... CELLULAR ENVIRONMENT INDUCED DEATH 1.2 ANTIBIOTIC INDUCED CELL DEATH 1.3 THE COMMON DEATH PATHWAY- GOING AGAINST THE DOGMA 1.4 MYCOBACTERIA- DO THEY SHOW THE COMMON PATHWAY? 13 20 MATERIALS AND...

Ngày tải lên: 02/10/2015, 17:15

... 15 and 30, HT-29 cells were used between Passage 13 and 22, CaCO-2 cells were used between Passage 25 and 50 and FHC cells were used between Passage and 13 3.2.1 Cell counting and plating of cells ... cancer cells and whether cell death induction and immunomodulation are involved In this preliminary study, the four human colonic cells were shown to have varying degree of cell death as well as cell ... with and 24 hours CR treatment……………………60 Fig 4.29 CaCO-2 cells with and 24 hours RAC treatment………………….60 Fig 4.30 HT-29 cells with and 24 hours CP treatment…………………… 61 Fig 4.31 HT-29 cells with and...

Ngày tải lên: 05/10/2015, 19:03

... remaining cells are endothelial cells, stellate cells, Kupffer cells and lymphocytes The immune cell comprises mainly phagocytic cells such as neutrophils and macrophages, lymphocytic cells such ... macrophages, lymphocytic cells such as NK cells, NKT cells and T cells The role of immune cells such as dendritic cells, CD4 and CD8 T cells, as well as T regulatory cells has been described in liver ... cells and NK cells 47 Figure TLR3 expressed by both the tumour cells and NK cells is associated with prolonged patient survival 48 Figure 10.TLR3 in HCC cells is functional and...

Ngày tải lên: 13/10/2015, 16:50

... proteins Bax and Bak, caspase activation and cell death [2,3] In addition, the physiological platelet agonist thrombin also induces Bid, Bax and Bak translocation to the mitochondria and endogenous ... (B) Mean values (and SD) obtained from four independent experiments *P < 0.01 versus control and zymosan-treated cells (Fig 5A) nor OPZ (Fig 5B) induced activation of caspases and at any time ... both platelet activation and death Results Characterization of morphological modifications induced by OPZ Treatment with OPZ induced two main modifications of platelet morphology: (a) cell remodeling...

Ngày tải lên: 16/03/2014, 04:20

... L(1–2); loop between sheets b-1 and b-2; L(2–3), loop between sheets b-2 and b-3 Asp57, Glu197 and Glu201 are residues involved in chitosan substrate binding at )2, )1 and +2 subsite, respectively ... cell density We thus estimated the number of falsepositive colonies recovered on this medium by mixing various proportions of the V148T chitosanase-expressing cells and chitosanase-negative cells ... CsnN174 (GH46) C-end of a-1 helix b-1 strand E36 (alternative general base) D40b (general base) T45 (water positioning) Loop between b-1 and b2 strands b-2 strand Hen egg-white lysozyme (GH22) T japonica...

Ngày tải lên: 16/03/2014, 04:20

... ligand -induced activation away from the TLR7 toward the TLR8 pathway, as observed in HEK cells expressing TLR7 and TLR8 and in primary human peripheral blood mononuclear cells [67] and mouse cells ... inhibition in AM14 B cells We created linear (18, 44, 46, and 48) and palindromic (1, 43, 45, and 47) INH-ODNs lacking the CCT element (45 and 46), GGG element (43 and 44), or both (47 and 48) Similar ... Hartmann G: Plasmacytoid dendritic cells, antigen, and CpG-C license human B cells for plasma cell differentiation and immunoglobulin production in the absence of T -cell help Blood 2004, 103:3058-3064...

Ngày tải lên: 09/08/2014, 14:21

... factor-alpha -induced NF-kappa B activation and intercellular adhesion molecule-1 expression in endothelial cells J Biol Chem 277, 3404–3411 Gloire G, Legrand-Poels S & Piette J (2006) NF-kappaB activation ... the febrile response, two classes of cytokines have been reported: endogenous pyrogenic cytokines (IL-1 and IL-6) and endogenous antipyretic cytokines (IL-10 and tumor necrosis factor-a) [50] In ... of NF-jB, PI3K ⁄ Akt, 5-LOX ⁄ FLAP and NADPH oxidase inhibitors on SEC1 induced pyrogenic cytokine production and fever index in rabbits Human PBMC (1 · 107 cellsÆmL)1) were pretreated with or...

Ngày tải lên: 19/02/2014, 00:20