activation and effector phases of the inflammatory response

Tài liệu Báo cáo Y học: Mutations in the docking site for cytochrome c on the Paracoccus heme aa3 oxidase Electron entry and kinetic phases of the reaction pptx

Ngày tải lên : 22/02/2014, 07:20

... hypothetical second site have been made here for the bacterial enzyme by stripping subunits III and IV off the native complex, and by further destroying a large part of the acidic lobe(s) of the ... on (a) the presence of subunits III Ó FEBS 2002 and IV in the Paracoccus enzyme, and by inference on the presence of cytoplasmically coded subunits of the mitochondrial enzyme as well, and (b) ... of the turnover number The reaction of cytochrome c oxidase shows a strict dependence of the turnover number on ionic strength We measured the oxidation of 20 lM horse heart cytochrome c by the...

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Báo cáo y học: "Suppression of the inflammatory response in experimental arthritis is mediated via estrogen receptor α but not estrogen receptor " pot

Ngày tải lên : 12/08/2014, 14:21

... cell types and the microenvironment involved impacting the class of the immune response Here, we focused on defining the contribution of ERα or ERβ to modulation of the inflammatory response in ... of inflammatory processes in experimental arthritis Materials and methods Mice and rats All the experiments were approved by the Animal Welfare Committee of Schering-Plough, Oss, The Netherlands ... suppress the inflammatory response There is still some controversy around the topic of additional ERs such as GPR30 [44] Our current study and the study by Engdahl and colleagues confirm the important...

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Centrifuge and numerical modelling of the seismic response of pile groups in soft soils

Ngày tải lên : 09/09/2015, 11:16

... CENTRIFUGE AND NUMERICAL MODELLING OF THE SEISMIC RESPONSE OF PILE GROUPS IN SOFT CLAYS ZHANG LEI (M Eng., SDU; B Eng., CQU) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF CIVIL ... (2013) and Others at the National University of Singapore Initial earthquake geotechnical research at the National University of Singapore (NUS) involves studying the seismic response of soft soil ... predictions for the maximum pile bending moments along the piles as well as the acceleration response at both the free-field ground surface and the raft top The numerical simulations were then extended...

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Báo cáo y học: "Inhibition of allogeneic inflammatory responses by the Ribonucleotide Reductase Inhibitors, Didox and Trimidox" pot

Ngày tải lên : 11/08/2014, 06:22

... Didox and Trimidox is not due to the cytotoxic effects of the treatment To determine if the lack of proliferation was a result of the toxicity of the compounds, we studied the effects of drug ... the ability of these compounds to inhibit the secretion of the crucial cytokines, IL-2, IL-6, TNF-a and IFN-g thereby reducing the collateral damage caused by the direct actions of these cytokines, ... IL-4, IL-10 and IL-13 The effects of Didox and Trimidox on Allogeneic MLR The following experiments were performed to analyze the effects of Didox and Trimidox in the complex interaction of allo-recognition...

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Báo cáo y học: "Apoptosis of resident and inflammatory macrophages before and during the inflammatory response of the virgin bovine mammary gland" docx

Ngày tải lên : 12/08/2014, 18:22

... regulating the lifespan in macrophages and whether apoptosis of these cells participates in the inflammatory response of the virgin mammary gland The aim of this study, therefore, was to confirm the ... major parts of these cells survive, monitoring inflammatory or other danger signals, and phagocytosing the cellular material of sloughed epithelial and other cells from ducts of mammary glands, as ... article as: Sladek and Rysanek: Apoptosis of resident and inflammatory macrophages before and during the inflammatory response of the virgin bovine mammary gland Acta Veterinaria Scandinavica 2010...

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Báo cáo y học: "The effect of glutamine infusion on the inflammatory response and HSP70 during human experimental endotoxaemia" pptx

Ngày tải lên : 13/08/2014, 11:23

... ASA and TPS conducted the study in the volunteers and drafted the manuscript OHM conducted the BMNC isolation and HSP70 Western blotting, with the assistance of TPS and PM GvH conducted the analysis ... level of HSPs and subsequent induction of otherwise lethal sepsis exhibited attenuated release of TNF-α and IL-6 and reduced mortality [21-23] Based on these findings, the positive effect of glutamine ... investigate the effect of glutamine on the response of TNF-α, IL-6, cortisol and white blood cell (WBC) subpopulations to a standardized inflammatory stimulus (intravenous injection of E coli endotoxin)...

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Báo cáo y học: "Lysis with Saponin improves detection of the response through CD203c and CD63 in the basophil activation test after crosslinking of the high affinity IgE receptor FcεRI" ppsx

Ngày tải lên : 13/08/2014, 13:22

... contributed to project design and writing of the manuscript RD contributed to the design of the study and writing of the manuscript All authors read and approved the final manuscript 10 11 12 ... Comparison of response of CD63 and CD203c with different lysis methods Degree of activation at varying concentrations of CRA1 The % positive cells at a given concentration are plotted against the amount ... Histograms of CD63 and CD203c expression on basophils and control cells Histograms of CD63 and CD203c expression on basophils and control cells Histograms of expression of CD203c (a & c) and CD63...

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Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf

Ngày tải lên : 19/02/2014, 12:20

... (M23L) by the addition of AAP, as described in the Materials and methods Completion of the hydrolysis was conﬁrmed by the disappearance of the (Met1)-ONC (M23L) signal in the MALDI-TOF mass spectra ... analyzed by MALDI-TOF MS The addition of the protease is considered the zero time point The relative intensities of the peaks from the mass spectra were used to calculate the fraction of Pyr-ONC (M23L), ... simultaneously interacts with the main phosphate of the substrate [32] The closer positioning of the N-terminus to the main protein body is essential for the activity and, together with the 87–104 disulﬁde...

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Báo cáo khoa học: Characterization of HbpR binding by site-directed mutagenesis of its DNA-binding site and by deletion of the effector domain pot

Ngày tải lên : 07/03/2014, 17:20

... assembled as described in the main text Relevant restriction sites and the direction of transcription for the hbpR and the luxAB genes are indicated (A) (B) Detail of the region of the calmodulin-binding ... of the hbpC promoter in Escherichia coli Results Mutagenesis of the HbpR-binding sites Sequence alignment of the UASs found in the HbpR operators in the hbpC and hbpD promoters deﬁned a set of ... (in which the mutations had been placed), was impaired both at the 3¢ end of the central regions R22 and R23, whereas the region R21 and the 5¢ part of R22 were again protected, as for the wild-type...

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Báo cáo khoa học: Crystal structure and solution characterization of the activation domain of human methionine synthase doc

Ngày tải lên : 23/03/2014, 09:21

... is the measure of the fluorescence intensity in the absence of the activation domain, E0 is the concentration of the FMN domain, L denotes the concentration of the activation domain added to the ... concentration of the activation domain and the curves show the best fit of the data to the quadratic Eqn (1) Table lists the calculated dissociation constants for the wild-type and D963E ⁄ K1071N alone and ... Titration of the activation domain of MS (both the D963E ⁄ K1071N mutant and wild-type proteins) with the FMN domain of human MSR resulted in a quenching of the fluorescence emission spectra of the...

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báo cáo hóa học: " Inhibition of microglial inflammatory responses by norepinephrine: effects on nitric oxide and interleukin-1β production" pdf

Ngày tải lên : 19/06/2014, 22:20

... in the presence of serum, and that also releases the butyryl group from the 5'-position The antiinflammatory effects of dbcAMP on NO production could therefore be due, in part, to production of ... levels of nitrite production as did wild type cells; and the inhibitory effects of NE were maintained both at the highest concentration of LPS (Figure 11C) and at the lower ones (not shown) Together, ... and of cell adhesion molecules [14] The effects of NE appear to involve activation of β-adrenergic receptors (β-ARs) and elevation of intracellular cAMP, and in most cases lead to suppression of...

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Báo cáo khoa học " Study on macrophage activation and structural characteristics of puriﬁed polysaccharide from the liquid culture broth of Cordyceps militaris " doc

Ngày tải lên : 28/06/2014, 11:20

... for at 70 ◦ C, then mixed with a 5× first-strand buffer, 10 mM dNTPs, and 0.1 M DTT The reaction mixture was further incubated for at 37 ◦ C, then for 60 after the addition of U of MuLV reverse ... oxide (NO), and other inflammatory mediators The production of NO, IL-1␤, and TNF-␣ is an important part of the immune response to many inflammatory stimuli (Porcheray et al., 2005) In the present ... analysis of CPSN Fr II and standard polymers according to the absorption maximum of the congo red-polysaccharide complex at various concentrations of NaOH For more details, see Section (C–H), and...

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Báo cáo lâm nghiệp: "Response of the Norway spruce (Picea abies [L.] Karst.) root system to changing humidity and temperature conditions of the sit" ppt

Ngày tải lên : 07/08/2014, 03:22

... planting of spruce on the very boundaries of its ecovalence and the climate change (weather course) over the recent years The weather course affects the condition of forest stands in the individual ... causes of the decline of spruce stands in five selected regions of the Czech Republic – from FAVZ to FAVZ in nutrient-rich and acidic sites With respect to the size of the paper and to the fact ... stands 3–37 years, Norway spruce at the boundary of its ecovalence The decline has been recorded in the last years The visual symptom of the injury in all the analyzed stands is yellowing of...

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Báo cáo khoa học: "Influences of DTC and zinc supplementation on the cellular response restoration in restrained mice" docx

Ngày tải lên : 07/08/2014, 18:21

... action of DTC is reflected in the accelerated process of thymus gland and spleen size reversion, restoration of the total number of cells of these two lymphatic organs, the percentage of CD4+ ... Results The effects of DTC with zinc ions interaction on the total number of thymocytes and splenocytes and weight of the thymus and spleen in restrained mice As reported in Table 1, the total ... number of thymocytes and splenocytes and the weight ratio of thymus and spleen of restrained mice markedly decreased as early as 24 h following the exposure to stress The suppressive effect of acute...

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Báo cáo y học: "Assessment of the emotional responses produced by exposure to real food, virtual food and photographs of food in patients affected by eating disorders" ppsx

Ngày tải lên : 08/08/2014, 23:21

... the room and to stand in front of the six plates covered by the red lids (the same used in the real food (RF) and photograph (PH) conditions) indicated by the yellow arrow on the right side of ... samples (AN and BN) into quartiles and selected the first (25% with the lowest scores on the ITC-SOPI) and the fourth (25% with the highest scores) A simple effect of the degree of presence on the STAI-S ... second hypothesis, we found an effect of subjects' degree of presence experienced in the VR condition on their level of perceived anxiety (STAI-S and VASA): the higher the sense of presence, the higher...

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Báo cáo khoa học: "Preliminary study of the monoterpene response of three pines to Ophiostoma clavigerum (Ascomycetes : Ophiostomatales) and two chemical elicitors" pot

Ngày tải lên : 08/08/2014, 23:22

... beetle and its associated fungus However, the nature and the origin of the chemical elicitor responsible for the stimulation of the parenchyma cells is not clear In a previous paper, we reported the ... Raffa and Berryman (1982a), Schuck (1982) and Delorme and Lieutier (1990) noted an increase of the total monoterpene content of phloem and sapwood in the induced reactions of P contorta, Abies grandis ... not to define the qualitative response of these species but rather to compare the terpene composition of responding tissues with that of unwounded tissues of the same tree Although there is a...

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Báo cáo y học: "Synergistic role of c-Myc and ERK1/2 in the mitogenic response to TGF-1 in cultured rat nucleus pulposus cells" ppsx

Ngày tải lên : 09/08/2014, 01:22

... represents the analysis of 10,000 events The histograms present typical results and the percentage of cells in G0/G1, S and G2/M phases are shown as the average of duplicated measurements Page of 12 ... protein itself and also downregulated the phosphorylation (activation) of ERK1/2 The results of these blot analyses reveal that the effect of the TGF1 signal can be mitogenic when c-Myc and phosphoERK1/2 ... high and constant level of c-Myc Also, the contribution of Ras/Raf/ERK prevented the rapid degradation of c-Myc by phosphorylation of the serine 62 residue in the N-terminal of cMyc [24] They...

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Báo cáo y học: " Multiple functions for CD28 and cytotoxic T lymphocyte antigen-4 during different phases of T cell responses: implications for arthritis and autoimmune disease" pot

Ngày tải lên : 09/08/2014, 01:23

... expression is independent of the proliferative history of the cell and is exclusively dependent on the time elapsed since the onset of activation of the T cell [11] The expression of surface CTLA-4 is ... regulator of CTLA-4 function and dissociates from CTLA-4 upon ligand binding The ligands are the dimeric CD80 and the monomeric CD86 Binding of CD80 to the divalent CTLA-4 leads to the formation of ... the CTLA-4 gene, is demonstrated by comparing human and mouse sequences: the homology of the DNA sequence is 78%, and that of the protein sequence is 74% Nevertheless, four polymorphisms of the...

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Báo cáo y học: "Increased circulating levels and salivary gland expression of interleukin-18 in patients with Sjögren''''s syndrome: relationship with autoantibody production and lymphoid organization of the periductal inflammatory infiltrate" pptx

Ngày tải lên : 09/08/2014, 01:23

... counted The histological evaluation of the number of foci, the presence of IL-18 in inflammatory foci and ducts, the degree of organization of the mononuclear aggregates, and the presence of germinal ... salivary gland of SS, ductal IL-18 expression was observed both in the presence and in the absence of IL-18-producing cells in the periductal inflammatory foci and was also found in the absence of a ... at RT The sections were rinsed in TBS and stained for anti-IL-18 as described above To verify the specificity of the staining, sections with omission of the first, the second, or both of the primary...

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Báo cáo y học: "Expression of the inflammatory chemokines CCL5, CCL3 and CXCL10 in juvenile idiopathic arthritis, and demonstration of CCL5 production by an atypical subset of CD8+ T cells" pps

Ngày tải lên : 09/08/2014, 07:20

... values of CCL5, and error bars one standard deviation Phenotype of CCL5+ T cells within the joint CD8+ T cells of the effector and memory compartments have been shown to express high levels of the ... producing the chemokine CCL5 [39,40] The memory population may be further divided by the expression or loss of expression of CCR7 [12] We and others have previously shown that the majority of T cells ... has extended our knowledge of the expression of the inflammatory chemokines CCL5, CXCL10 and CCL3 in JIA All three chemokines were present in SF and, in the case of CCL3 and CXCL10, a large gradient...

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