activated protein kinase studies
Báo cáo y học: "Stable expression of a recombinant human antinucleosome antibody to investigate relationships between antibody sequence, binding properties, and pathogenicity" doc
Ngày tải lên :
09/08/2014, 06:23
... the end of the experiment Throughout the experiment, proteinuria was assessed using Albustix (Bayer Diagnostics, Newbury, Berkshire, UK) Proteinuria was scored as negative or trace that is negligible; ... cells into SCID mice Mean human IgG in sera (ng/ml) (no of mice/group) Terminal proteinuria (range for group) Mean proteinuria (estimateda g/l) Mean day of death
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Báo cáo khoa học: A mouse model for in vivo tracking of the major dust mite allergen Der p 2 after inhalation docx
Ngày tải lên :
07/03/2014, 21:20
... proteins in the different mouse tissues A comparison between lung and kidney samples revealed dissimilar patterns of radioactively labelled proteins in these two organs (Fig 5) In the lung, protein ... being killed were run on SDS ⁄ PAGE Proteins were stained with Coomassie and radioactive protein bands were visualized by autoradiography Lung and kidney proteins from sensitized and nonsensitized ... the metabolism of 75Se-labelled proteins can readily be followed through identification of newly synthesized selenoproteins, as the major endogenous murine selenoproteins are few and relatively...
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Báo cáo khoa học: The N-terminal cysteine pair of yeast sulfhydryl oxidase Erv1p is essential for in vivo activity and interacts with the primary redox centre doc
Ngày tải lên :
17/03/2014, 10:20
... appearance of the mutated proteins turned towards the Fig Colours of purified Erv1p and mutant proteins Protein concentrations vary due to different expression levels of the mutant proteins in E coli ... the mutant protein once it is lost Calculation of the FAD content of the purified mutant proteins turned out to be difficult due to variations in FAD/ protein ratios of the recombinant proteins Control ... is C30S Because Erv1p exists as a dimeric protein in vivo it is possible that heterodimers between the temperature-sensitive protein and the C30S mutant protein lead to intramolecular complementation...
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Báo cáo hóa học: "Multicenter phase II study of matured dendritic cells pulsed with melanoma cell line lysates in patients with advanced melanoma" potx
Ngày tải lên :
18/06/2014, 16:20
... three allogeneic melanoma cell lines [3,4] These cell lines were selected because they express proteins that have been identified as common melanoma antigens and they are known to trigger CD8 ... performed pulsing IDD-3 with just one melanoma cell line lysate (M17) together with hepatitis B surface protein and tetanus toxoid [3] This pilot study demonstrated the immunogenicity of this vaccine ... and subcutaneous tissues, evaluable only by physical examination and not detected using imaging studies, were considered measurable if adequately recorded using a camera with a measuring tape...
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Báo cáo hóa học: " Are quantum dots ready for in vivo imaging in human subjects?" docx
Ngày tải lên :
22/06/2014, 18:20
... attaching surface proteins to QDs and the difficulty in precisely controlling the protein- to-QD ratios Both of these complications may result in QD conjugates with misaligned protein orientations ... mortalin, and chaperonin proteins [51, 59– 62] The cell membrane proteins and receptors that have been labeled with QDs include prostate specific membrane antigen (PSMA), HER kinases, glycine receptors, ... specific membrane antigen (PSMA), HER kinases, glycine receptors, serotonin transport proteins, p-glycoprotein, band protein, and many others [20, 21, 37, 38, 42, 44, 63–67] The excellent photostability...
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báo cáo khoa học: " Manufacture of IRDye800CW-coupled Fe3O4 nanoparticles and their applications in cell labeling and in vivo imaging" ppt
Ngày tải lên :
11/08/2014, 00:22
... IRDy800CW-MNPs Animal studies are indispensable to the clinical applications of nanoparticles The biodistribution, metabolism, clearance and toxicity of nanoparticles must be examined in animal studies prior ... increasingly important role in in vivo studies [28-33] Therefore, this study investigated the applicability of the IRDy800CW-MNPs in this field The in vivo studies were performed in a mouse model ... 1, 5, and 20 mg/kg, produced no pathological evidence of toxicity [60] Furthermore, the animal studies revealed that IRDye800CW and its conjugates were capable of fine in vivo imaging in small...
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báo cáo khoa học: "BRCAA1 monoclonal antibody conjugated fluorescent magnetic nanoparticles for in vivo targeted magnetofluorescent imaging of gastric cancer" ppsx
Ngày tải lên :
11/08/2014, 00:23
... interactive domain-containing protein 4B)], and identified its antigen epitope peptide SSKKQKRSHK [7,8] We also prepared BRCAA1 polyclonal antibody, and observed that the BRCAA1 protein exhibited over-expression ... [28], the targeting ability of as-prepared nanoprobes is dependent on RGD peptide Some studies show that HER-2 protein exhibits abnormal expression in 6-35% gastric cancer tissues [29,30], and has ... cancer biomarkers were reported Although HER-2 protein was confirmed to have positive expression in 6-35% of gastric cancer tissues [28-31], HER-2 protein also exhibits over-expression in many...
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Báo cáo y học: " Identification of unique reciprocal and non reciprocal cross packaging relationships between HIV-1, HIV-2 and SIV reveals an efficient SIV/HIV-2 lentiviral vector system with highly favourable features for in vivo testing and clinical usa
Ngày tải lên :
13/08/2014, 09:21
... limiting Gag polyprotein [13] These differences in the location of the major packaging determinants may contribute to the ability of viral mRNA to be cross packaged by a heterologous Gag protein The ... RNAprotein complex is then targeted to the plasma membrane where virion budding takes place Poly A pol ∆ Env RRE Figure packaging constructs Gag-Pol Gag-Pol packaging constructs such as the G protein ... known as the packaging signal or psi and the nucleocapsid (NC) domain of the Gag structural polyprotein This highly specific process results in the selection of unspliced viral mRNA from a high...
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Báo cáo y học: " A remission spectroscopy system for in vivo monitoring of hemoglobin oxygen saturation in murine hepatic sinusoids, in early systemic inflammation" doc
Ngày tải lên :
13/08/2014, 13:20
... decreases cellular high-energy phosphates leading to an early hepatocellular injury and dysfunction Studies of tissue oxygenation focusing on the relationship between microcirculatory disturbances...
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Báo cáo y học: "Human T-cell leukemia virus type 2 post-transcriptional control protein p28 is required for viral infectivity and persistence in vivo" ppt
Ngày tải lên :
13/08/2014, 05:20
... These biological studies have led the way for future studies that are needed to understand the function of p28 Such studies will entail identifying the functional domains of the protein involved ... Rex activity 6Kb 7Kb 8Kb 3’LTR pol Structural & enzymatic proteins pro env rex tax p28 rex p22/20 p10 Regulatory proteins Accessory proteins p11 Figure Organization of the HTLV-2 genome and coding ... been unable to detect p28 protein by Western blot [30] (Fig 2C and data not shown) We then determined the effect of exogenously over-expressed p28- and Δp28-AU1 tagged proteins on Tax-mediated...
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Báo cáo toán học: "Iodine Alters Gene Expression in the MCF7 Breast Cancer Cell Line: Evidence for an Anti-Estrogen Effect of Iodine" doc
Ngày tải lên :
08/08/2014, 17:20
... factor 15 interferon gamma receptor myristoylated alanine-rich protein kinase C substrate 3.3 2.7 2.7 2.5 2.5 2.4 2.2 2.1 2.0 hypothetical protein DKFZp434P055 oral cancer overexpressed tripartite ... (estrogen-inducible sequence) NM_003881 WISP2 WNT1 inducible signaling pathway protein 23 Cell Cycle genes NM_003258 TK1 thymidine kinase 1, soluble NM_002305 LGALS1 lectin, galactoside-binding, soluble, ... stress protein, in cellular response to DNA damage Mutat Res 2005, 569:133-143 Jia J, Li B, Jin Y, Wang D Expression, purification, and characterization of human tyrosyl-tRNA synthetase Protein...
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Báo cáo y học: ""Shock and kill" effects of class I-selective histone deacetylase inhibitors in combination with the glutathione synthesis inhibitor buthionine sulfoximine in cell line models for HIV-1 quiescence" doc
Ngày tải lên :
12/08/2014, 23:21
... a Jurkat cell clone (A1) fluorescent protein (GFP) by MS-275 and buthionine sulfoximine Stimulation of HIV-1 LTR-controlled expression of green fluorescent protein (GFP) by MS-275 and buthionine ... ACH-2 cells than in their uninfected counterparts and linked this phenomenon to the cytotoxicity of activated HIV-1 replication in lymphoid cells [16] In our experiments, three different class I HDACIs ... was confirmed by our experiments using Jurkat cell clones with an integrated green fluorescence protein (GFP)encoding gene under control of the HIV-1 LTR [17] In these Jurkat cell clones, GFP...
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INTERLEUKIN 6 RELEASE FROM t98g HUMAN GLIAL CELL LINE AS a PREDICTIVE MARKER FOR CHRONIC PAIN, AND THE CHARACTERIZATION OF SUBSTANCE(S) INVOLVED IN PAIN
Ngày tải lên :
02/10/2015, 17:15
... PBS with 0.1% v/v Tween-20 PGE2 Prostaglandin E2 PHN Post-herpetic Neuralgia PKA Protein Kinase A PKC Protein Kinase C xv PMSF Phenylmethylsulfonyl Fluoride PNS Peripheral Nervous System ppN/OFQ ... fibers or increase their responses to heat stimuli through an IL-1β receptor type I/tyrosine kinase /protein kinase C (PKC)dependent mechanism (Obreja et al., 2002) 21 1.2.3.3 Interleukin-6 (IL-6) ... injury or disease conditions, activation of microglia occurs first Activated microglia display a change in surface markers and membrane proteins, and this triggers the production and release of painenhancing...
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Báo cáo y học: "High level expression of apoptosis inhibitor in hepatoma cell line expressing Hepatitis
Ngày tải lên :
02/11/2012, 11:17
... surface protein [22] Besides those viral proteins, HBV expresses a small non-structure protean named X protein, its function is still unclear The role of HBV protein, particular of middle surface protein ... of HBV protein plays role in this alteration is unknown In an attempt, we have expressed the different HBV proteins such as surface proteins (LHBs, MHBs and SHBs), core protein and HBV X protein ... them by proteolysis [8] The activated executioner caspases cleave a number of structural and regulatory proteins, leading to apoptotic cell death [26] One kind of proteins that regulate cell...
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Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx
Ngày tải lên :
19/02/2014, 16:20
... technologies In marked contrast, studies addressing the effect that iron loading of hepatic cells has on the expression of immune recognition molecules have been scarce In vivo studies by Hultcrantz and ... mice against a recombinant denatured CD1d protein the capacity of this antibody to recognize native CD1d molecules is limited [26] Accordingly, further studies were performed using CD1d42 antibodies, ... not influence their expression (Fig 4B) CD1d protein and mRNA are upregulated in iron-loaded HepG2 cells Immunocytochemistry and immunofluorescence studies confirmed that Nor3.2-reactive CD1d is...
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Tài liệu Báo cáo khoa học: Identification of different isoforms of eEF1A in the nuclear fraction of human T-lymphoblastic cancer cell line specifically binding to aptameric cytotoxic GT oligomers ppt
Ngày tải lên :
21/02/2014, 00:20
... nuclear proteins and lg of polyclonal anti-eEF1A (lane 4), with nuclear proteins and 0.9 lg of polyclonal anti-eEF1A (lane 5), with nuclear proteins and lg of total CRS (lane 6), with nuclear proteins ... recombinant eEF1A protein (R eEF1A); lane 2, eEF1A protein from total nuclear extracts (NE eEF1A); lane 3, P1 band excised from an SDS/PAGE gel (P1) (B) P1 affinity for GT oligomer P1 protein, excised ... nuclear protein, Ran-GTP, was detected by Western blot on the same filters used to analyse eEF1A protein, by using a specific mAb (BD Pharmingen, CA, USA) Results Identification of eEF1A as a nuclear protein...
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Tài liệu Báo cáo khoa học: Insulin/protein kinase B signalling pathway upregulates metastasis-related phenotypes and molecules in H7721 human hepatocarcinoma cell line pptx
Ngày tải lên :
21/02/2014, 00:20
... such as receptor protein tyrosine kinase/ growth factor receptor binding protein 2-son of sevenless protein/ Ras/Raf/mitogen activated protein kinase kinase/mitogen activated protein kinase pathway ... Woodgett, J & Dedhar, S (1998) Phosphoinositide-3OH kinase- dependent regulation of glycogen synthetase and protein kinase B/AKT by the integrin-linked kinase Proc Natl Acad Sci USA 95, 11211–11216 ... Sable, C.L., Hemmings, B.A & van Obberghen, E (2000) Effect of phospho- inositide-dependent kinase on protein kinase B translocation and its subsequent activation Mol Cell Biol 20, 5712–5721 Hemming,...
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Báo cáo khoa học: Measuring enzyme activities under standardized in vivo-like conditions for systems biology pdf
Ngày tải lên :
06/03/2014, 09:22
... measured, i.e phosphofructokinase and hexokinase (HXK; EC 2.7.1.1) All enzyme activities were expressed as moles of substrate converted per minute per milligram of extracted protein Protein determination ... steady-state samples from a single chemostat culture Enzyme In vivo-like Vmax (mmolÆmin)1Æg protein) 1) Flux (mmolÆmin)1Æg protein) 1) HXK PGI PFK ALD TPI GAPDH PGK GPM ENO PYK PDC ADH 0.80 2.8 0.80 1.2 26 ... assays, in which cell extracts are diluted The crowded intracellular environment may affect protein protein interactions and thereby also the activities of the enzymes involved [58] As an indirect...
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Báo cáo khoa học: Activator-binding domains of the SWI ⁄ SNF chromatin remodeling complex characterized in vitro are required for its recruitment to promoters in vivo pot
Ngày tải lên :
07/03/2014, 00:20
... resonance energy transfer (FRET) to measure in vivo interactions between proteins fused to derivatives of the green fluorescent protein, the activation domain of the transcriptional activator Gal4 ... required a group of genes that are activated by the same activator We chose to study the GAL regulon, which is known to be regulated by the Gal4 activator protein We first tested a group of known ... activity is repressed by its association with the Gal80 repressor protein Upon addition of galactose to such cultures, the Gal3 protein antagonizes Gal80-mediated repression and Gal4 is immediately...
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Báo cáo khoa học: In vivo RNA interference in oyster – vasa silencing inhibits germ cell development pptx
Ngày tải lên :
07/03/2014, 00:20
... of protein samples, total protein extracts were quantified using a DC protein assay (Bio-Rad, Hercules, CA, USA), and adjusted to a final concentration of mgÆmL)1 Twelve micrograms of each protein ... from 0% to 83% In the same samples, the protein level of histone H3 (blot under the graph) was unchanged The control used for protein measurement is a pool of proteins from all control oysters injected ... protein level ranged from 15% to 100% In the same samples, the protein level of histone H3 (blot under the graph) was unchanged (D) OYVLG protein level month postinjection (D) The values presented...
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