... 74% ofthe cases; residue 186 an Asn in 87% ofthe cases; and residue 334 a His in 80% ofthe cases [3] In addition, residue 54 is a Pro in 89% ofthe cases The importance ofthe identity ofthe ... respectively) ofthe very unstable variants Q334H and G56S/Q334H At the routinely used pH of 8.1, there is only a slight effect of vitronectin on the rate of latency transition of PAI-1 wild-type, and the ... wild-type, and the use of this pH therefore allowed optimization ofthe difference in the effect of vitronectin on wild-type and the unstable variants The presence of vitronectin during the incubations...
... expressed as a percentage ofthe total radioactivity in the cells at the end ofthe loading period (time 0) and are the mean of three separate experiments Error bars show the SEM The efflux curves were ... about the effects of CMR on NF-jB activity in macrophages The composition ofthe diet is known to be important in the development of atherosclerosis [24–26], and a major dietary determinant is the ... fish oil, respectively) they also contained a complex mixture of fatty acids which reflects the composition ofthe parent oils and of physiological CMR derived from them [28] The triacylglycerol ⁄...
... of uncertainty ofthebiomass estimate in the young age-classes The uncertainty ofthebiomass estimate calculated with the age-dependent BEFs was highest in the youngest age-classes due to the ... especially for the possible presence of bias, before they can be used in the national inventories Furthermore, it is evident that the time series ofthe inventory, i.e thebiomass estimates ofthe earlier ... pine The uncertainty in thebiomass estimates of birch was high, since the number of birch stands was low in both the material used for formulation ofthe BEFs, and in the Swedish data on which the...
... 2) was the result, at least partly, ofthe acquisition ofthe G116E substitution in the CA The amino acid residue at the 116th position ofthe CA affects viral growth in the presence of TRIM5α ... position ofthe CA on its three-dimensional (3-D) structure, the 3-D model ofthe N-terminus ofthe CA was constructed by homology-modeling on the basis ofthe published crystal structure ofthe N-terminus ... capability ofthe virus in human cells Sequence analysis ofthe MA-CA region ofthe adapted virus revealed that the there was a G-to-E single amino acid substitution at the 116th position ofthe CA...
... 67 the regulation of proliferation, migration, and differentiation ofthe epithelial cells [2,12,18,19] Following the surgical treatment of adult periodontitis, the epithelial regeneration ofthe ... for the tools and facilitate the direct clinical view ofthe defect [fig 3-4] A full-thickness flap was elevated and the granulation tissue was removed showing the true extension and depth ofthe ... demonstrated a gingivitis grade of or Thanks to the bacteriostatic properties ofthe tested polymer, a more effective control ofthe surgical wound no bacterial contamination ofthe surgical site was observable...
... avoid the adsorption loss of phosphorus and other changes that occur in soil The experimental equipment was placed on the rooftop terrace ofthe Institute of Industrial Science, the University of ... under fluorescent lighting of 15 watts The distance from the fluorescent lamp to the top ofthe giant reeds was about 0.2 m at the start ofthe experiment The temperature in the experimental room ... placed on the leaf The amount of phosphorus in the leaves was calculated based on the count ofthe beta ray, capture efficiency, device efficiency, and a specific value ofthe reagent The phosphorus...
... fatty acids (LCFAs) These standards were used to obtain 56 kinds of fatty acids Therefore, the contents of each fatty acid were calculated based on the quantity of lipid, the content ratios of all ... the deciduous effect ofthe respective parts of Z japonica The δ13C in the roots and rhizomes seems to be larger than the δ13C in the leaves and stems For the same species of seagrass, when the ... designates the sum of all the fatty acids with < 5% content The three fatty acids 16:0, 18:2n6c, 18:3n3 were found > 15% in the leaves and stems of all the samples of Z - 104 - Journal of Water...
... cracking Figure shows the schematic diagram ofthe SJBG The SJBG consists ofthe gas producer, purification unit and the generator The gas producer is an 180Nm3/h gas production unit The purification ... efficiency ofthe gasifier without looking at the downstream processes and associated components Figure Schematic diagram ofthe system Johansson Biomass Gasifier Figure shows the photo ofthe 180Nm3/h ... conversion efficiency ofbiomass gasifiers is an important factor that determines the feasibility of using a biomass gasifier, hence the relevance of this work The findings of this work assisted...
... lack of domain B protruding out ofthe TIM barrel in the place of loop connecting the b3-strand to the a3-helix [9,21] Sharing domain B by rBATs and GH13 enzymes, and especially the sequence ofthe ... ofthe hcHAT proteins and the GH13 a-amylase family members The tree is based on the alignment of complete sequences and calculated including gaps The numbers represent the bootstrap values The ... and S Janecek Origin of rBAT and 4F2hc within the GH13 a-amylase family Fig The CSRs ofthe hcHAT proteins and the GH13 a-amylase family members A list ofthe abbreviations of proteins and enzymes...
... interaction between the flavin, Y308 and the nicotinamide of NADP+ is precisely tuned by selecting amino acids that face the other side ofthe tyrosine phenol ring The specific volumes ofthe above-mentioned ... arrangement by the influence of amino acids C266 and L268 These residues face the other side of this tyrosine and are members of a conserved loop (266CGLKG270) that shapes part ofthe FNR catalytic ... shown) Therefore, the amino acid changes introduced not prevent assembly ofthe prosthetic group and not impede the production of a folded protein, although, as mentioned above, they may affect the...
... this is the rst demonstration of FdGOGAT mRNAs in the cytoplasm on the periphery of chloroplasts and ofthe enzyme protein in the chloroplasts ofthe two cell types This spatial distribution of Fd-GOGAT ... 4B) The localization of GLU mRNAs in the vicinity ofthe vascular bundle of BSCs suggests a role of Fd-GOGAT in amino acid translocation Therefore, B 10 Activity àmolãh1ãg1 FW Localization of ... amino acids is tightly correlated with the expression ofthe genes involved [15] Thus, we analysed the diurnal expression ofthe genes encoding the enzymes of nitrogen assimilation in the second...
... 4¢-phosphopantothenic acid, CTP and cysteine The synthesis of PPC occurs in two half reactions starting with the formation of 4¢-phosphopantothenoyl-CMP (activation ofthe carboxyl group of 4¢-phosphopantothenate) ... synthetase CoaB (Eur J Biochem 271) 169 Fig Analysis ofthe enzymatic activity of His-CoaB R206Q and A276V The synthesis of 4¢-phosphopantothenoyl-CMP (–cysteine, upper part ofthe figure) and of ... and that the partial blocking ofthe Dfp (CoaBC) enzyme is overcome in this way The molecular basis for the conditional lethality ofthe dfp-707 mutant is that one amino acid residue ofthe N-terminal...
... ScPDC on the other hand involving the C-terminal part ofthe polypeptide chain (Fig 7) In the latter, differences in the conformation ofthe loop between Fig Stereo picture ofthe model ofthe a-carbanion/enamine ... catalytic residues The active site cavity in IPDC extends from the thiazolium ring ofthe cofactor to the surface ofthe protein The entrance ofthe active site cleft is covered by the C-terminal ... in all ofthe tetrameric PDCs of known three-dimensional structure Binding ofthe cofactors ThDP and Mg2+ The homo-tetrameric IPDC binds four molecules ofthe cofactors ThDP and Mg2+ The ThDP...
... with the nt sequence ofthe open reading frame (ORF) of their cDNAs and the corresponding aa sequences, in Table Summary ofthe purification of DNases I from the pancreases of three species of snake ... not The same was true for these mutants ofthe rat and mouse DNase I (Fig 3) These findings demonstrate that the nature ofthe amino acid at position 130 may generally and markedly affect the thermal ... regions of E climacophora DNase I cDNA were identical to those of E quadrivirgata, but 8.4% (16/189) ofthe entire nt sequence in the 3¢-UTR ofthe former was different from that ofthe latter The...
... 70–80% ofthe BSM was coupled collected at a rate of 0.6 mLÆmin)1 The column was washed with HSB until the A280 ofthe effluent was < 0.002 The column was further washed with LSB at °C until the ... in the hemolymph of several crustaceans has been well known since the beginning ofthe 20th century [34] An evaluation ofthe literature revealed that purification of lectin from the hemolymph of ... that the major binding site ofthe lectin was sialic acid on the surface of erythrocytes The binding specificity of crab lectin Inhibition studies with various sugars was helpful in deducing the...
... reduction of their PBN adducts within the network of interacting lipoyl moieties ofthe E2 core provides a good explanation for these phenomena Considering possible mechanisms ofthe inactivation ofthe ... Hartman for their advices concerning the EPR measurements at the beginning of this work Critical reading ofthe manuscript by Prof J Mieyal and Prof A J L Cooper and the interest of Prof G Gibson ... either 2-oxo acid and CoA or NADH Thus, the E3-bound FAD was responsible for the formation of radical species at the expense of either complex-bound or free dihydrolipoate or NADH Action of the...
... investigate the evolution ofthe enzymes ofthe pyruvate dehydrogenase (PDH) complex, the tricarboxylic acid cycle, and the glyoxylate cycle by examining the individual phylogenies ofthe 21 subunits ... [106], these cases are relatively easy to explain as above But if one considers the evolution of all ofthe enzymes ofthe pathway (Fig 4), it is clear that only about half ofthe enzymes ofthe ... to the cytosol ofthe host [3,83] For the tricarboxylic acid cycle, a complete transfer ofthe pathway to the cytosol would not work, because some of its enzymes are intergral components of the...
... enhancement of secondary structure ofthe fragment species, and these significantly modify the physical state ofthe fatty acid in solution The increase in the a-helical structure in the fragment ... Aggregation ofthe fragment was deduced from the turbidity ofthe last eluted fraction (Fig 1Bd) This would account for the low recovery of soluble OA complex of fragment 53–103 For the sake of comparison, ... fractions ofthe effluent from the column that were collected for further studies fatty acid [20] The conformational features of a-LA in the OA complex are those of a protein MG, and upon binding to the...
... (indicated in bold) The enrichment for a given GO category was computed as the ratio ofthe distribution ofthe amino acid- regulated genes* versus the distribution ofthe genes spotted onto the microarray** ... contribute to these multistep processes, including the metabolic conditions ofthe cell, nature ofthe stimulus, RNA binding factors and the sequence ofthe target mRNA [35] Another amino acid sensing ... microarray** *Percentage ofthe representation of one GO term among all the amino acid- regulated genes **Percentage ofthe representation of one GO term among all the genes present on the micro-array...
... arrangement of sHsps 5886 The alteration ofthe properties ofthe C-terminal extension also leads to significant changes to the structure and function of sHsps The chaperone activity of Hsp30C is ... examined the role of residues in the C-terminal extensions of other sHsps, notably aA- and aB-crystallin, the flexible regions of these proteins are unique and distinct from that of Hsp25 The a-crystallins ... indicative of a change in the polarity ofthe tryptophan environment [37] Therefore, the overall tryptophan environment was not significantly affected by substitution ofthe glutamic acid residues in the...