... activity in crude yeast extract as the untagged protein, indicating that the tag was not interfering with the protein activity. The tagged protein was purified and then used for further analysis. In the ... nkatÆmg )1 . The GLD1 pro- tein was tagged with a histidine tag at the N-terminal end by adding the coding sequence for six histidines to the end of the open reading frame, and then expressed in S. ... The tagged protein had a similar activ- ity in the crude extract as the nontagged protein, indi- cating that the tag did not affect the protein activity. The tagged protein was then purified and...
Ngày tải lên: 19/02/2014, 06:20
... to the setting, run them through the action and finally move on to the climax of the game. This method can enable players to begin playing the game before knowing all of the rules, letting them ... mindful of their own safety and the safety of others, including parties for whom they have a professional responsibility. To the fullest extent of the law, neither the Publisher nor the authors, ... vision on the game and the rest of the team. Instead, the designer should seek out the opinion of other team members. Use the expertise of your teammates to solve problems. But at the end of the...
Ngày tải lên: 16/03/2014, 21:27
báo cáo khoa học: "Speciation burst hypothesis : an explanation for the variation in rates of phenotypic evolution" pps
Ngày tải lên: 09/08/2014, 22:22
Báo cáo khoa học: N-Glycosylation is important for the correct intracellular localization of HFE and its ability to decrease cell surface transferrin binding pptx
... described in the secretion, stability and oligomerization of proteins [38,39], the bioactivi- ties of enzymes [40] and the binding affinities of ligands and receptors [41]. In many instances, ... absence of tunicamycin. Drug treatments resulted in the accumulation of forms of the mutant proteins that were of lower apparent molecular mass and of similar size to the unglycosy- lated form of the ... was func- tionally deficient in b2-microglobulin interactions and incapable of regulating transferrin binding, once again, reminiscent of the HFE-C282Y variant. Single and double mutants that undergo...
Ngày tải lên: 06/03/2014, 22:21
Báo cáo y học: "Multivariate explanatory model for sporadic carcinoma of the colon in Dukes’ stages I and IIa"
... pathway in patients with SCRC depending on the location of the tumour in the large intestine [31]. The use of LR for the observational studies continues to being authenticated by the bibliography, ... interest in the re- cords gathered by the different collaborators. The fruit of that qualitative examination was the rejection of a total of 9 controls and 3 cases at the defining moment of ... Service during 2003. From 2001 to 2003, the rest of the cases and controls in this investigation were collected in the centers of ori- gin (Table 1). Therefore, the time limits of our data...
Ngày tải lên: 03/11/2012, 11:34
LINH DAM NEW TOWN - SOLUTION FOR THE HIGH-DENSITY DEVELOPMENT OF NEW SETTLEMENTS IN THE SOUTH-WEST OF HANOI
... adjustments for Linh Dam Master plan in response to the need of mix uses, more accommodation and more outstanding architectural positions for Linh Dam, from the beginning of 2001,. According to the ... in the middle, still has the highest density). New Towns in the south-west of Hanoi (including Linh Dam) have been rapidly developed in many types and forms, contributing very much in the ... road and housing densities. Urbanization results in the expansion of administrative boundaries, stretching in various directions and leading to changes in land use, mainly at the periphery. The...
Ngày tải lên: 29/08/2013, 08:15
LINH DAM NEW TOWN - SOLUTION FOR THE HIGH-DENSITY DEVELOPMENT OF NEW SETTLEMENTS IN THE SOUTH-WEST OF HANOI
... factors (living and working environments in cities, home-town customs, working conditions and relating policies) were identified as the determinants affecting to jobs and daily life of female ... labors selling goods in the streets and in- waiting labor market have to work in unsafe environments; their daily life are rather poor. 22.86% female freelane labors live in boarding houses characterized ... jobs as family care, selling goods in the streets or in- waiting labor markets. Although working time is rather long (11- 13 hours/day) and continuously, their montly income average only ranges...
Ngày tải lên: 29/08/2013, 08:15
Tài liệu Action plan for the multi-level conservation of forest wetlands in the Mekong River Delta, Vietnam pdf
... provide information on the type and the location of wetland, the economic and ecological value of the wetland, and the type and incidence of human threat [2]. They are used as basis for the formulation ... awareness of economic and livelihood values in wetland biodiversity conservation sites; ▪ revising and upgrading wetland sustainability utility planning; and ▪ integrating information for wetland forest ... on forest fire in 2002 [7], about 8,212 ha of Melaleuca forest in the Upper and the Lower U Minh areas were burned, accounting for approximately 57% (17% in the Lower U Minh and 40% in the...
Ngày tải lên: 09/12/2013, 22:15
Developing a system of exercises to improve public speaking skill in interpreting for the 4th year students of english at vinh university
Ngày tải lên: 25/12/2013, 20:21
Tài liệu Báo cáo khoa học: A role for the intersubunit disulfides of seminal RNase in the mechanism of its antitumor action docx
... mechanism of antitumour action of BS-RNase is based on the ability of the protein to resist the neutralizing action of the cytosolic RNase inhibitor, a resistance due to the dimeric structure of the enzyme. ... exerted when the protein reaches the cytosol of the affected cells, where it degrades ribosomal RNA, thus blocking protein synthesis and leading cells to death. The current model proposed for the mechanism ... cannot fit into the horseshoe cavity of the inhibitor, whereas a monomeric form of the enzyme can, and is fully inhibited by CRI. Indeed, monomeric RNases lacking cytotoxic activity, such as bovine...
Ngày tải lên: 20/02/2014, 11:20
Báo cáo khoa học: Definition of the residues required for the interaction between glycine-extended gastrin and transferrin in vitro pptx
... 130% of the wild-type value [18]. The biochemical basis of the gastrin–transferrin inter- action is still unknown. Knowledge of the regions of transferrin required for the binding of gastrin, and ... remained that mutation of the iron-binding residues did affect gastrin binding, and that the observed binding was to the unmutated lobe. The observation that the extent of crosslinking was the ... gastrin, and of the regions in gastrin required for the interaction with transferrin, is obviously essential to a full understanding of the interaction. The independent involvement of iron [17] and nonamidated...
Ngày tải lên: 07/03/2014, 02:20
Báo cáo khoa học: Structural basis for the erythro-stereospecificity of the L-arginine oxygenase VioC in viomycin biosynthesis docx
... l-homoarginine and l-canavanine (Fig. 3A, Table 1). These findings can be explained by the coor- dination of l-arginine in the active site of VioC (Figs 4, 5 and 6B). As the stereochemistry of the Ca-atom ... established by the hydroxyl group of the side chain of Ser224 and the carboxamide group of the side chain of Gln137. Gln137 is suitably ori- ented to interact with the a-amino group of l-arginine. These ... concerning the adenylation domain (A-domain) specificity of VioF revealed b-ureidoalanine activation, leading to the proposition of a new model for the order of the NRPSs during viomycin biosynthe- sis...
Ngày tải lên: 16/03/2014, 02:20
Báo cáo khoa học: "Semantic Retrieval for the Accurate Identification of Relational Concepts in Massive Textbases" ppt
... expansion of text information has motivated the development of efficient methods of access- ing information in huge texts. Furthermore, user demand has shifted toward the retrieval of more precise and ... entry and examine the effects of using predicate argu- ment structures and ontological identifiers. The need for the discovery of relational con- cepts has been investigated intensively in Infor- mation ... that target information must be formalized beforehand and each system is de- signed for a specific task. For instance, an IE system for protein-protein interactions is not use- ful for finding gene-disease...
Ngày tải lên: 17/03/2014, 04:20
Báo cáo " Balance of rice for the Mekong Delta - Viet Nam to 2050 in terms of industrialization and climate change '''' doc
... output increased due to intensive investment and increasing the use of rice land, but the rate of declining rice land under the influence of climate change and industrialization plus increasing ... Analysis the changes of elements in the model In the original script of the model using actual values of the elements obtained from the secondary resources from the provinces and the primary ... hectares per year. The land is recovered mainly for the construction of urban, industrial and other projects. With the speed of the current land acquisition, in 2020 paddy land in the country is...
Ngày tải lên: 24/03/2014, 01:21
Protecting the Elderly in Times of Disaster: The Critical Need for Comprehensive Disaster Planning and Exercise Design doc
... Lack of understanding of what comprises a “vulnerable population”, and the implication for this group prior to, and during, a disaster. Area 2. Lack of adequate understanding and training of the ... future planning efforts and the exercising of these plans. Introduction Recent disasters in the United States, especially Hurricane Katrina, have proven the inadequacy of planning for the protection ... States. Great effort and extensive resources have been spent in preparing ourselves for this likelihood of Avian Influenza, and the possibility of Pandemic Influenza. The potential for pandemic is...
Ngày tải lên: 28/03/2014, 20:20
báo cáo hóa học: " Characteristic values of the lumbar load of manual patient handling for the application in workers’ compensation procedures" pot
... thanks for very helpful support, the advice and the realising of their long experience in the field of nursing and for their capable accomplishment of the patient handling tasks to Mrs. B B. Beck and Mrs. ... from the signals of the four force sensors in the force platform. The height of the measur- ing chair could be adapted according to the require- ments of the specific patient handling. Furthermore, footstep-bridges ... healthcare worker just standing at the bedside and waiting for the signal announcing the start of the measurement. Thereupon she was bending forward to the patient in the bed. In detail, caregiver’s...
Ngày tải lên: 20/06/2014, 00:20
báo cáo hóa học:" In vivo evaluation of a vibration analysis technique for the per-operative monitoring of the fixation of hip prostheses" pdf
... amplifier. *The circle indicates the place of the measuring hardware (presented in the left picture) behind the transparent wall. A member of the surgical team holds the shaker during the insertion ... man- uscript. Appendix The intra-operatively manufactured prosthesis In the IMP-procedure, the stem of the prosthesis is custom made for each individual patient, during the operation [27]. After reaming of the ... sys- tem for intra operative manufacturing and stability testing of hip prostheses. In Proceedings of TEHNOMUS XIII, the 13th Inter- national conference on New technologies and products in machine...
Ngày tải lên: 20/06/2014, 01:20
Báo cáo toán học: " Weak convergence theorem for the three-step iterations of non-Lipschitzian nonself mappings in Banach spaces" pptx
Ngày tải lên: 20/06/2014, 21:20
Báo cáo hóa học: " Procedure for the steady-state verification of modulation-based noise reduction systems in hearing instruments" doc
Ngày tải lên: 20/06/2014, 22:20
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