a web tool for the detection of splice modulating exonic variants

... Giliani S, Insalaco A, Al-Ghonaium A, Soresina AR, Loubser M, Avanzini MA, Marconi M, Badolato R, Ugazio AG, Levy Y, Catalan N, Durandy A, Tbakhi A, Notarangelo LD, Plebani A: Mutations of CD40 ... via Galaxy [53] SNPs were retained only if there was a full three-way alignment available, chimp and macaque bases were identical, and one of the human alleles matched the ancestral chimp-macaque ... 41:e96 128 Hashimoto S, Tsukada S, Matsushita M, Miyawaki T, Niida Y, Yachie A, Kobayashi S, Iwata T, Hayakawa H, Matsuoka H, Tsuge I, Yamadori T, Kunikata T, Arai S, Yoshizaki K, Taniguchi N,...

Ngày tải lên: 09/08/2014, 20:21

... et al other Listeria spp are classified to A, B, C, or D type flagella antigen; but L grayi only have the E type flagella antigen [1] According to the report by Vatanyoopaisarn et al [22], attachment ... Selection for MAb pair for a sandwich ELISA Each monoclonal antibody (MAb) was diluted in carbonate buffer (pH 9.6) to the concentration of 10 µg/ml and 100 µg of diluted MAb was added into ELISA plate ... method that has nearly same sensitivity as × 104 to 105 bacteria ml−1 [4], the detection limit of the sandwich ELISA using Pair1 antibodies, the combination of HRP labeled MAb 7A3 and MAb 2B1 coated...

Ngày tải lên: 07/08/2014, 18:21

... Human Human Human Bovine Dog Negative Negative Negative Negative Negative 󰠏 󰠏 40 Vijayarani Kumanan et al Specificity of the assay The specificity of the lateral-flow biosensor assay was evaluated ... Vijayarani Kumanan et al handle systems for the detection and quantification of RNA molecules [1,2,4,5] A biosensor is a lateral flow assay that provides visual or reflectance data within about ... assess the signal-to-noise ratios with a relatively large dynamic range and the highest signal obtainable The standard lateral-flow biosensor assay was run in triplicate with μl of synthetic DNA with...

Ngày tải lên: 07/08/2014, 23:22

... doi:10.1186/1751-0147-53-23 Cite this article as: Jacobson et al.: Evaluation of a blocking ELISA for the detection of antibodies against Lawsonia intracellularis in pig sera Acta Veterinaria Scandinavica 2011 53:23 ... for the serological analyses UE were responsible for the statistical analyses and drafted this part of the manuscript All the authors red and approved the manuscript Competing interests The authors ... are however available for the use in other laboratories A blocking ELISA for the detection of antibodies to L intracellularis has been developed and is commercially available [13] To ensure a...

Ngày tải lên: 12/08/2014, 18:22

... resulting mathematically integrated patterns of the abnormal indexes are then compared for their degree of abnormality to the abnormal index patterns in the reference database to reach a final diagnostic ... to an amplitude histogram, which generates a large inventory of normalized mathematical indexes of abnormality It is the pattern of these mathematical indexes of abnormality, obtained from analysis ... in the included trials and were analyzed using the same software and hardware located at the central server location in New York All MCG analyses in this database have been validated against the...

Ngày tải lên: 03/11/2012, 10:58

... influence a substrate has on its own removal It is obtained for all substrates of any elementary reaction The main point of the present section is that any Jacobian matrix element equals the sum of a ... oscillations The main result of such an analysis is that oscillations arise if the parameter k3 is the largest and the parameters k6 and k7 are the smallest in the system Oscillations in this system can ... constructed of an even number of negative paths and any number of positive paths Our approach has the advantage that it considers positive and negative interactions in a unified manner The implication of...

Ngày tải lên: 19/02/2014, 16:20

... metabolic pathways Most of the available tools aim to interpret the whole mass spectra rather than a sparse list of compounds differentially present between samples Other tools are available that ... 101–103 2094 A V Antonov et al 33 Okuda S, Yamada T, Hamajima M, Itoh M, Katayama T, Bork P, Goto S & Kanehisa M (2008) KEGG atlas mapping for global analysis of metabolic pathways Nucleic Acids Res ... from the KEGG atlas However, without quantitative analysis, there are no clues about the quality of these relations To fill the gap, we propose an analytical framework for the interpretation of...

Ngày tải lên: 16/03/2014, 01:20

Ngày tải lên: 19/03/2014, 00:20

... CTGGCTAACGACTACATCTCCAGRGAYGARCT-3' 5'-GGCAGTTTCAAGGCTGTGAATTTYTTYGARCG-3' 5'-CCGTAAGAAATGGTGGTCCTGACRAAYTGNGG-3' 5'-CCGTAGGCGATGGTCGTCCTAACRAAYTGNGG-3' 5'-TACAAAATACAGCGAGTGATANATRAARCA-3' ORF ... A TGATCTGTAGTCCCCATGTGTCC T T A RV1 RFHVMm RFHVMn KSHV C A T CACA T.G GAGA C T GA GG.GCCCAATT AC GT C.C.GCGG.G.TATTT AG.CAGGCT A T CACA G GAGA C T GACGGAT CCTGGT GCGCGTAA.C A ... used the RV2 assay to detect novel RV2 rhadinovirus homologs in both the spleen of a crab-eating macaque (Macaca fascicularis) and the lymphocytes of a baboon (Papio cynocephalus) The standard...

Ngày tải lên: 18/06/2014, 22:20

... CTGGCTAACGACTACATCTCCAGRGAYGARCT-3' 5'-GGCAGTTTCAAGGCTGTGAATTTYTTYGARCG-3' 5'-CCGTAAGAAATGGTGGTCCTGACRAAYTGNGG-3' 5'-CCGTAGGCGATGGTCGTCCTAACRAAYTGNGG-3' 5'-TACAAAATACAGCGAGTGATANATRAARCA-3' ORF ... A TGATCTGTAGTCCCCATGTGTCC T T A RV1 RFHVMm RFHVMn KSHV C A T CACA T.G GAGA C T GA GG.GCCCAATT AC GT C.C.GCGG.G.TATTT AG.CAGGCT A T CACA G GAGA C T GACGGAT CCTGGT GCGCGTAA.C A ... used the RV2 assay to detect novel RV2 rhadinovirus homologs in both the spleen of a crab-eating macaque (Macaca fascicularis) and the lymphocytes of a baboon (Papio cynocephalus) The standard...

Ngày tải lên: 20/06/2014, 04:20

... enables a high number of cavities on each chip and facilitates the performance of many independent assays on one plate The funnelshaped cavities avoid the appearance of meniscuses, and therefore, ... participated in their fabrication, and wrote the main parts of the manuscript GO carried out the transfection of the reporter cell line and was involved in the preparation of the manuscript AS ... promoter activations that is based on the analysis of individual cells within a population It has been described that the physical properties of nanoscale materials can interfere with the analysis of...

Ngày tải lên: 21/06/2014, 00:20

... generate estimates of the noise covariance matrix with more variance when primary data are used More precisely, the variance of the noise covariance matrix estimate and then the associated performance ... (called primary observation vectors) For example the secondary observation vectors may correspond to samples of data associated with another range than the range of the detected target in radar ... R(nT), C(nT), s TNAR available R, C on sec data TNAR available R, C on sec + prim data No TNAR No TNAR available R, C on sec data TNAR available R, C on sec + prim data No TNAR - 29 - Receivers...

Ngày tải lên: 21/06/2014, 00:20

... Capture probe 5-GAGGAGGATGAAATAGATGGTCCAGCTGG ACAAGCAGAACCGGACAGAGCCCATTACAATAT TGTAACCTTTTGTTGCAAGTGTGACTCT ACGCTTCGGT-3 Secondary probe 5-GGAGCGACCCAGAAAGTTACCACAGTTATGC ACAGAGCTGCAAACAACTA-3 ... hybridization assay method only require extraction of DNA of the samples and simple incubation as well as magnetic separation, which has a good acceptability for any average lab assistant Table Comparison ... on the basis of the cutoff value Detection of HPV-16 with QDs and superparamagnetic nanoparticle-based hybridization The rationale of QDs and superparamagnetic nanoparticle-based hybridization...

Ngày tải lên: 21/06/2014, 01:20

... emaN ecneuqeS eborP esreveR drawroF eborP esreveR drawroF eborP esreveR drawroF '3-pxGCCAATTTCAGCCCAGGCACAAAm-'5 '3-TYCGGTTYGGGACCATGTT-'5 '3-AACTGTACAGRAGGACGTAGA-'5 '3-TAGCGCATGGTGGGCAACCTCCA-'5 ... 55/lizarb/oriezurc/4 2A rof ecneuqes ehT sniarts 21 eht rof dezylana saw noiger tegrat ehT )ASU ,ratsanD( erawtfos ratsanD eht gnisu dengila dna )1 elbaT( A C aisA 2TAS O O O O O O O O 55/lizarB/oriezurC/4 2A ... '3-TAGCGCATGGTGGGCAACCTCCA-'5 '3-CTGAARATGGGKACCTGCG-'5 '3-GTSCGYTTSTGYCGCACAA-'5 '3-px CCATGGACTTCCCGTAGGAATCGGACAm-'5 '3-CCATTGTRCGCTGTGAGC-'5 '3-CACGACCCCAACGTGTGT-'5 B2 redaeL SERI noigeR seborp dna sremirp cificeps-VDMF...

Ngày tải lên: 07/08/2014, 18:21

... keyword values Up to 10 additional keywords can be added and their keyword values are automatically calculated When performing a search, the textual information for each human gene stored in the table ... be a gene candidate The application can be accessed from the RatMap home page [7] or directly at http://ratmap.org/cgc Comparison of manual evaluation with CGC ranking To estimate the ability of ... containing rat genes and polymorphic DNA markers was used http://ratmap.org/ gene_mapping_data/integrated_linkage_maps/ For each QTL a pair of rat genes (obtained from the integrated linkage map)...

Ngày tải lên: 09/08/2014, 06:22

... between Fl and P2) are also heterogeneous AA or AB animals (BC1) and AB or BB animals (BC2) with proportions 1/2, 1/2 The statistical analysis of the data obtained from these populations was clearly ... preliminary analysis of available data; and iv) retrospective studies of old experiments without marker information may be important for various valuable The basis for population genetics was established ... and C26, and C3 and C5 The proportion of F2s to backcrosses increased between C30 and C35, C40 and C44, and C50 and C54 The major gene was characterized for each of these cases by an effect of...

Ngày tải lên: 09/08/2014, 18:21

... that enables visualization of transcriptome data onto a linear map of an annotated bacterial genome and at the same time highlights additional features, such as putative regulatory sequences and ... 95:14863-14868 Saeed AI, Sharov V, White J, Li J, Liang W, Bhagabati N, Braisted J, Klapa M, Currier T, Thiagarajan M et al.: TM4: a free, open-source system for microarray data management and analysis ... 30:59-61 Salgado H, Santos-Zavaleta A, Gama-Castro S, Millan-Zarate D, deposited research References reports Acknowledgements Our analyses of transcriptome data in relation to the activity of specific...

Ngày tải lên: 09/08/2014, 20:20

... distally along the shoe), medial (greater medial than lateral wear at the heel and forefoot), which may indicate excessive pronation, or lateral (greater lateral than medial wear at the heel and forefoot), ... range of each measure across included footwear was also reported Intra-rater and inter-rater reliability for all categorical data was evaluated using percentage agreement, and kappa (κ) statistics ... reliability for all categorical items, use of these items from the tool to assist clinical footwear assessment can be recommended for a range of populations Qualitative evaluation of the tool and...

Ngày tải lên: 10/08/2014, 21:23

... by a small sample size, the limited representativeness of participants, the lack of a comparison, and the assessment of intermediate outcomes For example, we have not evaluated the impact of the ... Hôtel Dieu, Paris Partner manager – Pierrre Durieux Italy: Unit of Clinical Governance, Agenzia Sanitaria Regionale (Regional Health Care Agency) of Emilia-Romagna, Bologna Partner manager – Roberto ... Italy: Center for the Evaluation of Effectiveness of Health Care (Ce.V.E .A. S.), Modena Partner manager – Alessandro Liberati The Netherlands: Centre for Quality of Care Research, University of...

Ngày tải lên: 11/08/2014, 05:22

... well managed if they are to generate useful knowledge Various web- based Page of 12 software platforms are available for managing and making use of metabolomics data These software platforms include ... Resonance data Bank (BMRB) [35] are also useful for metabolite identification by NMR Databases of this type may be seen as knowledgebases rather than integrated tools for data management, analysis and ... consultation For example, all protocols are Page of 12 collected in PDF format files, as such files are already available as part of the quality assurance approach operating in most laboratories:...

Ngày tải lên: 11/08/2014, 11:21