... NMR protocol is an important tool to study paramagnetic proteins Plastocyanin is a good model system to address features of paramagnetic copper proteins in terms of assignment strategy Indeed, ... recycle and polarization transfer delays allows to identify 11 resonances that clearly experience a substantial gain in signal intensity when comparing a diamagnetic HSQC experiment with a tailored ... of amino acids in the protein data base and NMR data indicates that amino acids have various phi propensities in the random coil conformation J Mol Biol 254, 322–333 16 Bertini, I., Cowan, J .A. ,...
Ngày tải lên: 08/03/2014, 08:20
... were added into each well and the plate was incubated at 37°C for 30 minutes After shaking out the samples, washing each plate six times with 1× TBST, and slapping the plate face down onto a clean ... purchased from the experimental animal centre of Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences Mice were maintained in isolation rooms in filter top cages and any ... Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, 250100, China 3Division of Swine Infectious Diseases, State Key Laboratory of Veterinary...
Ngày tải lên: 12/08/2014, 04:20
Báo cáo khoa hoc:" A simple way to distinguish bed clothing contamination in a whole body bone scan: a case report" ppsx
... body, to eliminate or reduce the uptake of radioactivity absorbed into the body, and cleaning and decontaminating dry and wet surface, equipment and clothing Figure ing contamination activity at ... her bed clothing, the abnormal tracer uptake disappeared Case-2 A 45 year old woman, with a history of adenocarcinoma of the right breast and a mastectomy performed two years ago, was referred ... conditions, including hot and humid or cramped working environments, can cause contamination [2] There are several methods to control these factors For example, decontaminating different anatomical sites...
Ngày tải lên: 11/08/2014, 10:22
báo cáo hóa học: " A refined in vitro model to study inflammatory responses in organotypic membrane culture of postnatal rat hippocampal slices" potx
... Sanz O, Acarin L, Gonzalez B, Castellano B: NF-kappaB and IkappaBalpha expression following traumatic brain injury to the immature rat brain J Neurosci Res 2002, 67:772-780 Hawiger J: Innate immunity ... anti-inflammatory stimuli during the culture Next, we wanted to see if either proinflammatory histone deacetylase inhibitor TSA or anti-inflammatory NF-κB inhibitor helenalin could influence the LPS-induced ... (TSA, 20 nM), a well characterised histone deacetylase inhibitor [25] and as an anti-inflammatory stimulus we used a known NF-κB inhibitor helen- Histological methods For revealing microglia,...
Ngày tải lên: 19/06/2014, 22:20
Báo cáo khoa hoc:" The chicken as a model to study microchromosomes in birds: a review" potx
... degree of chromatin compaction possible to obtain characteristic banding patterns for each pair (68], it is im- However, by using DAPI or chromomycin A3 staining, it has been suggested that most of ... Myakoshina Y.U., Chelysheva L .A. , Solovei LV., Gaginskaya E.P., Chiasmata on lampbrush chromosomes of Gallus gallus domesticus Cytogenetic investigations of recombination frequency and linkage group ... them as ancestral chromosomes although they are very rare in fish and batracians Indeed, they could have been inherited from a common ancestor of the vertebrates, as they can be encountered in...
Ngày tải lên: 09/08/2014, 18:21
Báo cáo sinh học: " A simple algorithm to estimate genetic variance in an animal threshold model using Bayesian inference" docx
... was calculated as: h = a + a e AnimB: Same model as AnimA, except that additive genetic variance was sampled using the new algorithm as described above Heritability was calculated as in model ... algorithm (AnimB) Here, mixing was much faster, and the samples were within a reasonable parameter space, given an input heritability of 0.20 Finally, the same dataset was analyzed using a standard ... replicates, the AnimA model resulted in genetic variance drifting towards infinity (both using standard Gibbs sampling and a random walk algorithm) However, the problems associated with animal models...
Ngày tải lên: 14/08/2014, 13:21
A simple introduction to working with LVM
... hda1, hda2, and hda3 are all physical volumes We'll initialize hda3 as a physical volume: root@lappy:~# pvcreate /dev/hda3 If you wanted to combine several disks, or partitions you could the same ... hda1 Boot Primary Linux ext3 [/] 8000.01 hda2 Primary Linux swap / Solaris 1000.20 hda3 Primary Linux 31007.57 Here I have a 7Gb root partition which contains my Debian GNU/Linux installation ... be able to see it included in the output of vgscan: root@lappy:~# vgscan Reading all physical volumes This may take a while Found volume group "skx-vol" using metadata type lvm2 Now that we have...
Ngày tải lên: 18/09/2012, 10:12
A simple route to annihilate defects in silicon nanowires
... down to  10À3 Torr, argon was fed into the tube as a protective gas and the furnace temperature was increased to 1100°C for h 1100°C was used as the annealing temperature because nanowires react ... nanowires react to become SiO vapor and disappear above this temperature [21] During annealing, a total pressure of 740 Torr was maintained Afterwards, small pieces of the specimen were taken o directly ... under an inert ambient HRTEM investigation of the microstructures in the nanowires after annealing showed that stacking faults and micro-twins in the as-grown Si nanowires were annihilated Raman...
Ngày tải lên: 16/03/2014, 15:03
Báo cáo khoa học: "A Simple Measure to Assess Non-response" docx
... Rodrigo, Anselmo Pe˜ as, and Felisa Verdejo n 2011 Evaluating Question Answering Validation as a classification problem Language Resources and Evaluation, Springer Netherlands (In Press) Tetsuya Sakai ... Seattle, Washington, USA, August 6-11, 2006, pages 525–532 Tetsuya Sakai 200 7a On the Reliability of Factoid Question Answering Evaluation ACM Trans Asian Lang Inf Process., 6(1) Tetsuya Sakai 2007b ... Felisa Verdejo n 2008 Evaluating Answer Validation in Multi-stream Question Answering In Proceedings of the Second International Workshop on Evaluating Information Access (EVIA 2008) Alvaro Rodrigo,...
Ngày tải lên: 17/03/2014, 00:20
Báo cáo khoa học: "A Probability Model to Improve Word Alignment" ppt
... A state in this space is a partial alignment A transition is defined as the addition of a single link to the current state Any link which would create a state that does not violate any constraint ... similar results using the same initial alignment and search algorithm with an alternative model Without using any features, our model is similar to IBM’s Model 1, in that they both take into account ... texts In Proceedings of the 4th Speech and Natural Language Workshop, pages 152–157 DARPA, Morgan Kaufmann Rebecca Hwa, Philip Resnik, Amy Weinberg, and Okan Kolak 2002 Evaluating translational...
Ngày tải lên: 17/03/2014, 06:20
a simple method to synthesize nanowires titanium dioxide from layered titanate particles
... (Wako) and TiO2 (ST-01, Ishihara Sangyo Kaisha LTD.) in the stoichiometrical ratio 1:3 The powders were mixed together and repeatedly ground in an agate mortar, and calcined at 1000 °C for h in ... of (a) the raw material Na2Ti3O7 and (b) product containing lots of nanowires Fig TEM images of the sample containing lots of nanowires (a) low magnification, (b, c) high magnification Scheme An ... resulted in weakened static interaction between neighboring [TiO6] octahedral sheets As a result, the layered compound Na2Ti3O7 particles were gradually exfoliated In the meantime, Na+ in Na2Ti3O7 was...
Ngày tải lên: 19/03/2014, 16:47
The Weapons Mix Problem - A Math Model to Quantify the Effects of Internetting of Fires to the Future Force pptx
... having to run the preprocessor again to create the input to the math solver The ability to make quick changes to the scenario also enables exploratory analyses and quick-reaction analyses Table ... also may lead to an overestimation of the damage to targeted systems) This too was not a limitation to the IOF Allocator but rather of the incoming data Finally, the formulation is one-sided and ... This is a means to aggregate the data somewhat to reduce the run-time of the analysis Moving to a unit-based approach rather than a cell-based approach would be the next step in formulating the...
Ngày tải lên: 23/03/2014, 02:20
Báo cáo hóa học: " A simple route to vertical array of quasi-1D ZnO nanofilms on FTO surfaces: 1D-crystal growth of nanoseeds under ammonia-assisted hydrolysis process" pptx
... Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor, Malaysia 2College of Engineering, Universiti Tenaga Nasional, 43000, Kajang, Selangor, Malaysia Department of Materials Chemistry, Graduate School ... the increasing of ammonia On the basis of available free-standing quasi-NRs, the diameter was seen to decrease from 30 nm to 15 nm for ammonia concentration increasing from 36 to 360 mM, inferring ... films, in which no absorption band appeared in this region Since we used FTO on glass as the substrate, which normally produces an artificial wave pattern at the glass-FTO interface due to an internal...
Ngày tải lên: 20/06/2014, 22:20
Báo cáo hóa học: "A Simple Method to Synthesize Cadmium Hydroxide Nanobelts" pptx
... sealed, warmed up at a speed of 8C/min and maintained at 100 8C for h, and was then cooled to room temperature on standing The white precipitate was filtered off, washed with absolute ethanol and distilled ... graphitemonochromatized high-intensity Cu Ka radi˚ ation (k = 1.541784 A) The accelerating voltage was set at 50 kV, with 100 mA flux at a scanning rate of 0.06°/s in the 2h range 10–80° The X-ray photoelectron ... distilled water for several times, and then dried in vacuum at 40 8C for h X-ray diffraction (XRD) patterns were carried out on a Japan Rigaku D/max rA X-ray diffractometer equipped with graphitemonochromatized...
Ngày tải lên: 22/06/2014, 01:20
Báo cáo khoa học: "Development of a new branchiness index ASIX – A simple tool to describe branchiness in young deciduous forest stand" pps
... tree quality Hakkila [12] and Hakkila et al [13] used similar indices to ASIX to estimate branch amount Table II Calculation of branch thickness with a special Asix Initial spacing “narrow spaced” ... These kind of disadvantages caused by using categorical variables can be avoided by the use of features on interval scales These interval scale variables must be created so that the results are independent ... create another architectural model The ASIX values are proposed as an additional, very easy, tool to describe and compare tree quality The periodical comparison of the ASIX-values is able to...
Ngày tải lên: 08/08/2014, 14:22
Báo cáo y học: "A cohort study of accidents occurring in mentally handicapped patients living in institutions" pot
... the univariate analysis to have P < 0.1, using a backward modelling strategy As some patients had several accidents (or as an individual who has had an accident may be more likely to have more), ... the patient All information was entered into a database designed for this purpose using FileMaker Pro for Windows (FileMaker, Santa Clara, CA, USA) Patient-related variables were entered into the ... considered as being at high risk of suffering accidents, a finding which should be taken into account when establishing the composition of care groups and training care staff Another relevant finding...
Ngày tải lên: 08/08/2014, 23:21
osophila as an emerging model to study metastasis doc
... resulted in excessive proliferation and metastasis [9,10] Scrib is an adaptor protein containing multiple PDZ domains (which enable binding to a variety of other proteins); it is localized to basolateral ... larvae gene encodes a protein that contains WD40 repeats (predicted to be involved in protein-protein interactions) and discs large encodes a member of the membrane-associated guanylate kinase ... (MAGUK) family that contains multiple PDZ domains known to be involved in targeting signaling proteins to the cell membrane Pagliarini and Xu [10] therefore asked whether the metastatic behavior...
Ngày tải lên: 09/08/2014, 20:20
báo cáo khoa học: "A simple technique to position patients with bilateral above-knee amputations for operative fixation of " docx
... the patient is important in achieving and maintaining fracture reduction while not causing skin injuries Generally, the foot of the affected limb is put into a boot, and applying traction to this ... UK Authors’ contributions AA was involved in collecting patient details, reviewing the literature, and drafting the manuscript as the main author AD was involved in reviewing the literature and ... securing the padded stump to the inverted traction boot; the stump and knee act as a pseudo foot and ankle, thus allowing traction to be applied to the limb In the case of an above-knee amputation,...
Ngày tải lên: 11/08/2014, 02:22
Báo cáo khoa hoc:" Applying the Stages of Change model to Type 2 diabetes care in Trinidad: A randomised trial" pptx
... variable measured was HbA1c level All HbA1c samples were tested at the same laboratory in the San Fernando hospital, Southwest Regional Health Authority in Trinidad Other variables recorded included ... was a simultaneous increase in the number of patients in the action and maintenance stages (collectively) at the end of the study In that publication application of a stage of change model based ... able to move to setting a date to start the appropriate exercise behaviour from initially being in the contemplation stage; four (4) patients each were able to move from preparation to action stage...
Ngày tải lên: 11/08/2014, 07:21
báo cáo khoa học: "Brachypodium distachyon: a new pathosystem to study Fusarium head blight and other Fusarium diseases of wheat" pdf
... participated to Bd detached leaf assays AS and PN took part in designing and supervising the study and participated in drafting the manuscript All authors have read and approved the final manuscript ... plant model available, Arabidopsis thaliana, because it is ideally suited to laboratory studies and there are extensive genetic and genomic resources available [20] Floral and foliar bioassays ... detection and quantification was performed using an ELISA competitive immunoassay (AgraQuant®, Romer Labs Singapore Pte Ltd) according to the manufacturer’s recommendation Inoculation, incubation and...
Ngày tải lên: 11/08/2014, 11:20