a reaction center complex associated with light harvesting complexes

... Biotechnologies) and goat anti-mouse IgG alkaline phosphatase conjugate (Novagen, Madison, WI, USA) were used as primary and secondary antibodies The absorbance was measured using a microplate reader (GMI, ... molecular masses of approximately 90, 55, 50 and 45 kDa Western blot analysis using anti-synaptotagmin as the primary antibody revealed that one 65 kDa band from the 0.1 m NaCl eluate is synaptotagmin, ... nonspecific binding Goat anti-GST IgG (Amersham Pharmacia Biotech) and rabbit anti-goat IgG alkaline phosphatase conjugate (Sigma) were used as the primary and secondary antibodies, respectively...

Ngày tải lên: 23/03/2014, 13:20

... Mukaiyama et al B BSA holo-NPAS2 ∆ F (Hz) holo-NPAS2 holo-NPAS2 holo-NPAS2 holo-NPAS2 a p o- N PAS apo-NPAS2 apo-NPAS2 apo-NPAS2 BSA apo-NPAS2 ∆ F (Hz) A Characterization of bHLH-PAS -A of NPAS2 ... was mixed with 2, or lm bHLH-PAS -A domain or 16 lm apoPAS -A domain using a stopped-flow apparatus at 25 °C There was a concomitant increase in the absorbance at 421 and 419 nm and a decrease at ... complexes with circadian and hypoxia factors Proc Natl Acad Sci USA 95, 5474–5479 Oishi K, Miyazaki K, Kadota K, Kikuno R, Nagase T, Atsumi G, Ohkura N, Azama T, Mesaki M, Yokimasa S, et al (2003)...

Ngày tải lên: 30/03/2014, 11:20

... GCA TTT TTT ATC AGC AAG CCC GTT AAA G C TTT AAC GGG CTT GAT GCT AAA AAA TGC ATA CGA GAT GCG G C CGC ATC TCG TAT GCA TTT TTT AGC ATC AAG CCC GTT AAA G C TTT AAC GGG CTT GCT GCT AAA AAA TGC ATA ... GAT TGC AGC AAT GAT AAC CGC C G CCT TCT TTC CGA TCA GCT TAC CGG GCT CTT G C AAG AGC CCG GTA AGC TGA TCG GAA AGA AGG C C TTT AAC GGG CTT GCT GAT AAA AAA TGC ATA CGA GAT GCG G C CGC ATC TCG TAT ... GGA ATG ATC ACC GGT GAT CAT TCC GCC CAT AGC GTG TCC GAA CAG CAC GTG CTG TTC GGA CAC TGT ATG GGC GGA ATG ATC AC GT GAT CAT TCC GCC CAT ACA GTG TCC GAA CAG CAC G GCG GTT ATC ATT GCT GCA ATC CAG...

Ngày tải lên: 30/03/2014, 13:20

... when analyzed separately as well as analyzed as a haplotype Especially in the sub group of patients ≤60 years old and in patients with non-abdominal and non-pulmonary sepsis focus the association ... Cerami A, Bucala R: MIF is a pituitary-derived cytokine that potentiates lethal endotoxaemia Nature 1993, 365:756-759 Calandra T, Bernhagen J, Mitchell RA, Bucala R: The macrophage is an important ... malarial anemia J Infect Dis 2009, 200:629-637 Dhanantwari P, Nadaraj S, Kenessey A, Chowdhury D, Al-Abed Y, Miller EJ, Ojamaa K: Macrophage migration inhibitory factor induces cardiomyocyte apoptosis...

Ngày tải lên: 18/06/2014, 15:20

... significance for this particular TNF-α gene variant have been demonstrated so far In general, an association between a specific disease and an allele can be interpreted either as a causal relation ... subgroups of COPD patients without and with radiological emphysema * Patients without radiological emphysema Patients with radiological emphysema (n = 32) (n = 127) Age (year) Sex (male/female) FEV1% ... technically acceptable spirometric manoeuvres was used Statistical Analysis Data are presented as mean ± standard deviation (SD) The Mann-Whitney U test was used for differences in age and FEV1...

Ngày tải lên: 12/08/2014, 18:20

... with AFM (raw images) Areas of different crystal lattices are indicated by dashed lines (A) Two types of zig-zag (areas and 2), disordered (area 3) and dimer (area 4) lattices (B) Zig-zag lattice ... (OTG) can greatly increase the crystal size Detergent removal using bio-beads can be achieved at a much higher rate than with dialysis [26] On the basis of AFM imaging of both the periplasmic and ... configurations can be obtained within one preparation AFM images allow the differences in protein packing and interaction within the membrane to be visualized Within the packed lattices, we find a new...

Ngày tải lên: 18/02/2014, 18:20

... cytochrome c oxidase from Paracoccus denitrificans Nature 376, 660–669 Tsukihara, T., Aoyama, H., Yamashita, E., Tomizaki, T., Yamaguchi, H., Shinzawa-Itoh, K., Nakashima, R., Yaono, R & Yoshikawa, S (1995) ... bo3 exhibits a broad Soret peak at 425 nm and bands at 530 and 560 nm in the dark Upon irradiation with a Xe lamp (Fig 4) or with an excimer laser after 15 laser flashes at 308 nm with an intensity ... evacuated to 8–10 mbar residual pressure The sample containment, maintained at °C, was purged with dry air to minimize absorbance by water vapor A water-cooled globar was used as source of radiation,...

Ngày tải lên: 21/02/2014, 15:20

... Buchnania lanzan extract administration increases the life span of rats with hepatocellular carcinoma D G Reddy, R Kartik, V C Rao, K M Unnikrihsnan and P Pusphangadan Laboratory of Ethnopharmacology, ... Department of Pharmacology, NBRI, Lucknow, UttarPradesh India E-mail: dayanandr@yahoo.com The effect of Buchnania lanzan bark extract administration after induction of hepatocellular carcinoma ... circadian signalling network using automated measurements of physiology and microarray analysis These data have allowed is to demonstrate why plants have circadian clocks and to quantify and characterize...

Ngày tải lên: 23/03/2014, 15:20

... immunoprecipitation with anti-FLAG IgG Western blot analyses with antibodies against PMCA, as a marker for the cell membrane, and extracellular signalregulated kinase ⁄ (ERK1 ⁄ 2), as a marker for ... (21 aa) and an intracellular portion (567 aa), containing a kinase homology (KH) domain, the dimerization domain and the C-terminal catalytic GC domain [1,7] In the absence of ligand, GC -A forms ... concentrations are associated with significant changes in blood pressure [6] The GC -A receptor consists of an extracellular ligand-binding domain of approximately 441 amino acids (aa), a short membrane-spanning...

Ngày tải lên: 29/03/2014, 09:20

... leaf mass per area (LMA) and leaf area ratio of the shoot (LARshoot , leaf area divided by the dry mass of stems + branches + petioles + leaves); (4) leaf area of individual leaves to calculate ... internode length; D/H, diameter/height ratio; LARshoot , leaf area ratio; number of leaves; Ea , light harvesting efficiency; MLA, mean leaf area; LMA, leaf mass per area) and the scores of the first ... summer and annual total precipitation; TJ /TA : mean of January and August temperatures, respectively Distribution and climatic data were taken from several sources for European [6, 18] and North American...

Ngày tải lên: 07/08/2014, 16:20

... vessels or coronary arteries and there was no pleural or pericardial effusion Arterial bloods gas analysis revealed a mild acidosis (pH 7.34 with a base excess of -5.7) and an elevated lactate level ... organ ischemia can be managed through revascularization grafts such as axillary- femoral bypass Endovascular stenting without entry closure for type A dissection has the risk of cardiac tamponade ... right axillary and bilateral common femoral approaches, a 150 mm covered stent graft (Medtronic, Santa Rosa, USA) was deployed into the thoracic aorta, distal to the left subclavian artery A further...

Ngày tải lên: 10/08/2014, 10:20

... the administration of anti-neoplastic drugs and the appearance of sarcoidosis [6-8] Although our patient manifested sarcoid reaction rather than sarcoidosis, it remains unknown whether sarcoid reaction ... Dermatology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan 2Surgical Oncology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, ... deoxyglucose uptake in sarcoidosis measured with positron emission tomography Eur J Nucl Med 1994, 21:297-305 13 Takanami K, Kaneta T, Yamada T, Kinomura S, Yamada S, Fukuda H, Takahashi S: FDG PET...

Ngày tải lên: 10/08/2014, 23:21

... FDAapproved drugs on the market for the treatment of RLS Now ropinirole and pramipexole, both dopamine agonists, are available Unfortunately these drugs can cause insomnia, nausea, dyspepsia, and ... designed to have a “support group” character A recent meta-analysis [27] assessing the placebo effect in RLS treatment studies found a substantial placebo response associated with RLS treatment This ... Pathophysiology, and Pharmacology Pharmacol Rev 1991, 43(2):109-142 23 Hawkins D, Abrahamse H: Phototherapy - a treatment modality for wound healing and pain relief African Journal of Biomedical Research 2007,...

Ngày tải lên: 11/08/2014, 03:21

... FDAapproved drugs on the market for the treatment of RLS Now ropinirole and pramipexole, both dopamine agonists, are available Unfortunately these drugs can cause insomnia, nausea, dyspepsia, and ... designed to have a “support group” character A recent meta-analysis [27] assessing the placebo effect in RLS treatment studies found a substantial placebo response associated with RLS treatment This ... Pathophysiology, and Pharmacology Pharmacol Rev 1991, 43(2):109-142 23 Hawkins D, Abrahamse H: Phototherapy - a treatment modality for wound healing and pain relief African Journal of Biomedical Research 2007,...

Ngày tải lên: 11/08/2014, 07:20

... obstetric patients.[1-3] In our review of literature there have been no report of severe anaphylaxis to ranitidine in a patient with pancreatitis Demirkan et al found only cases of anaphylactic reaction ... Hewitt PB: Anaphylactoid reaction to ranitidine in an obstetric patient Anaesthesia 1992, 47(4):360-1 Greer IA, Fellows K: Anaphylactoid reaction to ranitidine in labour Br J Clin Pract 1990, ... Natsch S, Vinkins MH, Voogt AK, Mees EB, Meyboom RHB: Anaphylactic reactions to proton-pump inhibitors Ann Pharmacother 2000, 34:474-6 Demirkan K, Bozkurt B, Karakaya G, Kalyoncu AF: Anaphylactic...

Ngày tải lên: 11/08/2014, 10:22

... Table Association of AKI with hospital mortality: results of the unadjusted and adjusted Fine and Gray modelsa SHR univariate analysis (95% CI) P value SHR multivariate analysis (95% CI) No AKI ... Silva S, de Almeida E, Abreu F, Martins C, Carmo JA, Lacerda JF, Prata MM: An assessment of the RIFLE criteria for acute renal failure following myeloablative autologous and allogeneic haematopoietic ... Comparisons of AKI patients according to their maximum RIFLE class were based on c2 tests for categorical data and on one-way analysis of variance or the Kruskal-Wallis test for continuous data as...

Ngày tải lên: 14/08/2014, 08:21

... 1145-9 Tamura N, Ogawa Y, Chusho H, et al Cardiac fibrosis in mice lacking brain natriuretic peptide Proc Natl Acad Sci USA 2000; 97: 4239-44 Mukoyama M, Nakao K, Saito Y, et al Human brain natriuretic ... Japanese Circ Res 2000; 86: 841-5 13 Nakayama T, Soma M, Rahmutula D, Ozawa Y, Kanmatsuse K Isolation of the 5'-flanking region of genes by thermal asymmetric interlaced polymerase chain reaction ... like to thank Dr Y Watanabe and Dr Y Izumi for collecting the samples, and Ms H Tobe, M Nakamura, and K Sugama for their technical assistance This work was supported financially by a grant from...

Ngày tải lên: 26/10/2012, 10:04

... Yamaguchi H, Sasaki K, Satomi Y, Shimbara T, Kageyama H, Mondal MS, Toshinai K, Date Y, Gonzalez LJ, Shioda S, Takao T, Nakazato M, Minamino N Peptidomic identification and biological validation of ... Cruz Biotech, CA) The assay was developed using a stabilized HRP substrate All samples were analyzed in the linear range of the ELISA using over-expressed human Vgf as a standard Assessment of ... Lange DJ, Voustianiouk A, Macgrogan D, Ho L, Suh J, Humala N, Thiyagarajan M, Wang J, Pasinetti GM A ketogenic diet as a potential novel therapeutic intervention in amyotrophic lateral sclerosis...

Ngày tải lên: 03/11/2012, 10:52

... can get acquainted with the ballot more quickly and shorten the task over each sample evaluation We compared this alphabetized approach with a randomized attribute presentation and found that ... chocolate, vanilla ice cream, fried chicken and mashed potatoes and gravy Pizza and chocolate produced the strongest emotions based on Analysis of Variance The terms active, adventurous, affectionate, ... Sensation seeking and positive affect 1.4 Facial scaling Another approach to measuring emotions has been the use of facial scaling A number of different systems for facial scaling have recently appeared...

Ngày tải lên: 03/04/2013, 21:07

... TCATCAT-3¢; rOMM-64-IV, 5¢-CGCGGATCCGCCCCT GTTAATGATGGAACC-3¢ and 5¢-CGCCTCGAGCTAA GAAGACTGGGCTGCCAG-3¢; rOMM-64-V, 5¢-CGCGG ATCCAGGCAAGATTTTAAGCATCCA-3¢ and 5¢-CGCC TCCACCTAAGAGGCATCCTTGTCCAC-3¢; ... 5¢-CGCCTCCA CCTAAGAGGCATCCTTGTCCAC-3¢; rOMM-64-II, 5¢CGCGGATCCACCGTAGACACTTATGATATA-3¢ and 5¢-CGCCTCGAGCTAAGAGTCAGCTTGCACGTC-3¢; rOMM-64-III, 5¢-CGCGGATCCGCTGATGTGACCAGT GATGAC-3¢ and 5¢-CGCCTCGAGCTATTTGGGCTCTT ... DNA contamination in the total RNA was confirmed by lack of amplification of a b-actin mRNA fragment by PCR using a pair of primers (5¢-ATCACCATCGGCAACGAGAG-3¢ and 5¢-TGGAGTTGTAGGTGGTCTCGTG-3¢) without...

Ngày tải lên: 18/02/2014, 17:20