a photolytic precursor of glutamate
Synthetic studies towards sieboldine a and a macrocyclic precursor of bielschowskysin
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Ngày tải lên: 09/09/2015, 10:15
Báo cáo khoa học: A novel mechanism of V-type zinc inhibition of glutamate dehydrogenase results from disruption of subunit interactions necessary for efficient catalysis doc
... concentration of the substrate glutamate was examined In these experiments a fixed concentration of NADP+ of 250 lM was used and the Ki for zinc was determined at a series of fixed glutamate concentrations ... relative quantitation was achieved using the ratio of the height of the emerging peak to that of the undigested GDH For MALDI-TOF calibration purposes, BSA was used as a standard and was diluted ... substrates (glutamate or norvaline) and zinc impact the overall stability and local flexibility of the enzyme As shown in Table 4, the addition of zinc to enzyme alone causes a small increase in thermal...
Ngày tải lên: 05/03/2014, 23:20
Báo cáo khoa họcRe-engineering the discrimination between the oxidized coenzymes NAD+ and NADP+ in clostridial glutamate dehydrogenase and a thorough reappraisal of the coenzyme specificity of the wild-type enzyme docx
... Km and the 0.3% NAD+ contamination in mm NADP+ is far below Km Nevertheless, a rate 200 of the rate in a standard NAD+ reaction may be anticipated A detailed re-analysis of the steady-state ... authentic NAD+ sample revealed total similarity of the fragmentation pattern The negative portion of the spectrum displayed a fragment at m z 540, along with a small amount of parental molecule ... was not observed in our batches of NAD+ Reassessment of coenzyme specicity of clostridial glutamate dehydrogenase In light of the discovery of variable contamination of the commercial NADP+ samples...
Ngày tải lên: 06/03/2014, 00:20
Báo cáo khoa học: Assessment of porcine and human 16-ene-synthase, a third activity of P450c17, in the formation of an androstenol precursor doc
... porcine and human assays using preg as a substrate, an additional peak of elution appeared at 15 (panels C and D, respectively) This additional peak coincides with the elution time of nonlabeled ... performed transfection assays with increasing amounts of DNA fragments encoding cyt b5 and monitored the formation of androstadienol and DHEA As shown in Fig the stimulation of DHEA and androstadienol ... a more profound effect on the synthesis of androstadienol More precisely, an increase of androstadienol formation was observed at a cyt b5/P450c17 ratio of : (Fig 5) The activity reached a maximum...
Ngày tải lên: 08/03/2014, 08:20
Báo cáo khoa học: Crystal structure of the halotolerant c-glutamyltranspeptidase from Bacillus subtilis in complex with glutamate reveals a unique architecture of the solvent-exposed catalytic pocket docx
... was amplified by PCR from the plasmid pCY167 (Suzuki H & Yamada C, Unpublished), using forward primer 5¢-CATATGGATGAGTACAAACA AGTAGATG-3¢ and reverse primer 5¢-GGATCCTCGAG CTCATTTACGTTTTAAATTAATGCCGAT-3¢ ... significance of the lid-loop in GGT catalysis During the preparation of this article, the crystal structures of Bacillus anthracis CapD, a GGT-related enzyme, in the absence and presence of a glutamate ... we analyzed its surface potential The waterbinding capacities of glutamate and aspartate have been reported to be 7.5 and 6.0 molecules per amino acid, respectively, whereas those for asparagine,...
Ngày tải lên: 22/03/2014, 21:20
Báo cáo khoa học: Hepatocyte growth factor activator is a serum activator of single-chain precursor macrophage-stimulating protein potx
... processing of pro-MSP in a dose-dependent manner Immunoblot analysis using an anti-MSP IgG revealed a band of approximately 60 kDa, presumably the a chain of mature MSP (Fig 1A) Generation of a band of ... (Qiagen, Tokyo, Japan) The following primers were used: b-actin: forward, 5¢-TGACAGGATGCAGAAGGA GA; reverse, 5¢-GCTGGAAGGTGGACAGTGAG; CD68: forward, 5¢-TCTACCTGGACTACATGGCGGTGG; reverse, 5¢-ACATGGCCCGAAGTGTCCCTTGTC ... growth factor Structural similarity of the protease precursor to blood coagulation factor XII J Biol Chem 268, 10024–10028 Hayashi T, Nishioka J, Nakagawa N, Kamada H, Gabazza EC, Kobayashi T, Hattori...
Ngày tải lên: 29/03/2014, 23:20
Báo cáo lâm nghiệp: "Changing electrophoretic patterns of glutamate dehydrogenases and aspartate aminotransferases in a few tree species under the influence of ectomycorrhization" potx
... Glutama dehydrogenases in ectomycorrhizas of spruce (Picea excelsa L.) and beech (Fagus sylvatica L.) New Phytol 111, 683-692 Khalid A. , Boukroute A. , Botton B & Martin F (1988) The aspartate aminotransferase ... glutamine synthetase /glutamate synthase in ammonia assimilation by Aspergillus nidulans J Gen Microbiol 123, 1235-1242 Lea P.J & Miflin B.B (1974) An alternative route for nitrogen assimilation ... cortical region including host cells and fungal hyphae revealed identical isoforms, while no activity was found in the peri- pheral mycelial layer (Table II) Conclusion In all the associations...
Ngày tải lên: 09/08/2014, 04:20
A Designated Center of Academic
... of thread that calls it – The DLL uses the stack of the calling thread and the virtual address space of the calling process – The DLL allocates memory from the virtual address space of the calling ... Systems Programming 10 2/27/2008 Advantages of Using DLL Some advantages over static linking – Smaller program image – Save system memory, since multiple processes can share a single copy of the ... Last Class Memory Management – Overview – Heap management – Memory-mapped files – Dynamic link libraries CS222 - Systems Programming 2/27/2008 Today’s Class Memory Management – Overview...
Ngày tải lên: 12/09/2012, 14:38
Cambridge.University.Press.A.Student.Grammar.of.Spanish.Feb.2006.pdf
... -ie and -sis are feminine: 13 A STUDENT GRAMMAR OF SPANISH -ma (el) asma la cama la crema (M) la estratagema la forma asthma bed cream stratagem form la gema la lima la trama la yema agility anxiety ... uncouth man, brute head of an organization 21 A STUDENT GRAMMAR OF SPANISH calavera c´ mara a caza cura espada facha g´nesis e gu a ı ma˜ ana n meta pareja recluta vig a ı skull camera hunt cure ... siguiente parrafo en voz alta y en clase, e indica el uso de la sinalefa Al escribir ´ las palabras, pon un , o sea subrayando el espacio entre las palabras apropiadas o dentro de una palabra: A trav´...
Ngày tải lên: 21/09/2012, 10:39
A study to indicate the importance of consumer based-brand equity on consumer perception of brand (a case study of fast food restaurants).pdf
... MacDonald can be said to have a larger degree of dispersion of data around the mean than Max hamburger in those attributes Max hamburger had a larger standard deviation than MacDonald in these attributes“its ... was clear and well dressed neat and trained staffs” Therefore MacDonald can be said to have a larger degree of dispersion of data around the mean than Max hamburger in those attributes Max hamburger ... jingle: a slogan is a visible feature of a brand There can be a strong link between a slogan and a brand The slogan and jingle are powerful and can be a great change for a brand • Be different and...
Ngày tải lên: 24/09/2012, 17:19
Báo cáo y học: "Excess circulating angiopoietin-2 is a strong predictor of mortality in critically ill medical patients"
... be statistically significant at a 10% level in the univariate analysis and subjected to multivariate Cox regression analysis: lactate, APACHE II score, SOFA score and circulating Ang-2 (Table ... Critical Care Vol 12 No Kümpers et al package (SPSS Inc., Chicago, IL, USA) and the GraphPad Prism software (GraphPad Prism Software Inc San Diego, California, USA) Figure Results Decreased Ang-1 and ... coefficient and linear regression analysis was performed after logarithmic transformation of Ang-2 values (logAng-2) The primary outcome studied was 30-day survival and was calculated from the day of...
Ngày tải lên: 25/10/2012, 10:31
Báo cáo y học: "Electronic patient record use during ward rounds: a qualitative study of interaction between medical staff"
... Intensive care unit The ICU, consisting of 25 critical care beds, is part of a specialist hospital that concentrates on all aspects of adult cardiothoracic care Approximately 70% of admissions are patients ... detailed data on particular aspects of a patient's condition and treatment – are accessible via tabs displayed across the top of the summary screen as shown in Figure A multidisciplinary team at ... place of a paper record? Given the complex nature of interaction of multidisciplinary communication in an ICU, we have chosen to triangulate three types of qualitative data: video-based interaction...
Ngày tải lên: 25/10/2012, 10:31
Báo cáo y học: "Surgical and medical emergencies on board European aircraft: a retrospective study of 10189 cases"
... board European aircraft Materials and methods This study originates from the surgical department of an academic teaching hospital (Department of General and Visceral Surgery, Augusta Krankenanstalt, ... compare the MFK of a large national European national airline with that of a lowcost (no-frills) carrier Although the national European airline had excellent equipment, intravenous medications and ... cases, 4.9%), patients with cardiac irregularities may profit from an on-board AED as part of the MFK The same is true for patients with a suspected myocardial infarction (34 cases, 0.3%) Apart...
Ngày tải lên: 25/10/2012, 10:31
Báo cáo y học: "Eosinopenia is a reliable marker of sepsis on admission to medical intensive care units"
... 51:189-197 A ssaoui Y, Zeggwagh AA, Zekraoui A, Abidi K, Abouqal R: Validation of a behavioral pain scale in critically ill, sedated, and mechanically ventilated patients Anesth Analg 2005, 101:1470-1476 ... Zeggwagh AA, Abidi K, Madani N, Abouqal R: Measurement properties of the short form 36 and health-related quality of life after intensive care in Morocco Acta Anaesthesiol Scand 2007, 51:189-197 A ssaoui ... of data NM helped to draft the manuscript, and participated in the acquisi- Page of 10 (page number not for citation purposes) tion of data AZ participated in the coordination of the study AAZ...
Ngày tải lên: 25/10/2012, 10:35
Báo cáo y học: "A severe coarctation of aorta in a 52-year-old male: a case report"
... of surgical repair of coarctation of the aorta in patients older than 50 years Ann Thorac Surg 2001; 72: 2060– 2064 Discussion Aortic coarctation is a congenital vascular lesion typically diagnosed ... Campbell M Natural history of coarctation of the aorta Br Heart J 1970, 32:633-640 Jenkins NP, Ward AR Coarctation of the aorta: natural history and outcome after surgical treatment QJM 1999, 92:365-371 ... Vermeersch P, Paelinck B, Van den Heuvel P, Van den Branden F Aortic coarctation: a rare and unexpected cause of secondary arterial hypertension in the elderly Cathet Cardiovasc Diagn 1996, 39:71-74...
Ngày tải lên: 25/10/2012, 11:40
Báo cáo y học: "Intrathecal siRNA against Toll-like receptor 4 reduces nociception in a rat model of neuropathic pain"
... sequence of rat TLR4 (GenBank accession NM_019178) were: 5’-CUACCAACAGAGAGGAUAU-3” (siRNA1), 5’-GUCUCAGAUAUCUAGAUCU-3’ (siRNA2), 5’-GAGCCGGAAAGUUAUUGUG-3’ (siRNA3) All siRNAs were chemically synthesized ... system (Amersham Pharmacia Biotech, Uppsala, Sweden) Histone3 (Sigma, St Louis, MO, USA, 1:500) was used as an internal control Statistical analyses Data are expressed as mean ± standard deviation ... once daily for days, starting from day before CCI surgery Evaluation of tactile allodynia and thermal hyperalgesia The paw withdrawal latency (PWL) to radiant heat and paw withdrawal threshold (PWT)...
Ngày tải lên: 25/10/2012, 11:48
Báo cáo y học: "A pilot study of rizatriptan and visually-induced motion sickness in migraineu"
... 2001;41:604-5 Prakash S, Chavda BV, Mandalia H, Dhawan R, Padmanabhan D Headaches related to triptans therapy in patients of migrainous vertigo J Headache Pain 2008;9(3):185-8 Marcus DA, Furman JM Prevention ... Female Female Female Female Female Female Female Aura Aura No Aura Aura Aura Aura Aura Vertigo Vertigo Non-Vertigo Non-Vertigo Vertigo Vertigo Non-Vertigo Motion Sickness History Actual Visual ... RB, Pascual J, Goadsby PJ, et al Effect of rizatriptan and other triptans on the nausea symptom of migraine: a post hoc analysis Headache 2001;41(8):754-63 32 Krymchantowski AV, Bigal ME Rizatriptan...
Ngày tải lên: 26/10/2012, 09:57
Báo cáo y học: " Self-reported sickness absence as a risk marker of future disability pension. Prospective findings from the DWECS/DREAM study 1990-2004"
... was stronger among men (OR=3.13) than among women (OR=2.19) (Table 2) Additional analysis treating days of sickness absence during 1990 as a continuous variable showed a clear trend of increase ... considerably younger than the official retirement age, and to ensure a maximum age of 59 during follow-up: Alternative labour market exit options in terms of voluntary early retirement is available ... Ministry of Employment, the Ministry of Social Affairs and the Ministry of Education DWECS was conducted in 1990, and featured a random sample drawn from the Central Population Register of Denmark of...
Ngày tải lên: 26/10/2012, 10:03
Báo cáo y học: "A comparative analysis of antibody repertoire against Staphylococcus aureus antigens in Patients with Deep-Seated versus Superficial staphylococcal Infections"
... clinical isolate of S aureus from the two groups of patients were obtained from clinical bacteriology laboratory, department of Medical Microbiology, PGIMER, Chandigarh, India A standard strain of ... Martin RR, Greenberg SB, Wallace RJ Staphylococcal teichoic-acid antibodies Lancet 1979;1(8118):731 Ayyagari A, Pal N Antiribitol-teichoic acid antibody (ARTA) in diagnosis of deep seated Staphylococcus ... Gram-positive and Gram-negative bacteria tested 3.5 Anti-staphylococcal antibodies react with PG and TA We observed that levels of anti-staphylococcal IgG antibodies were very similar when total...
Ngày tải lên: 02/11/2012, 11:08