a new method for fast purification of recombinant h3 h4 tetramers

... force calculation – a significant calculation part of FMM on GRAPE Remaining parts of the paper are organized as follows In section we gives a summary of the FMM and related algorithms as well as ... Computational Physics 87 (1990) 161 [9] T Amisaki, S Toyoda, H Miyagawa, K Kitamura, Development of hardware accelerator for molecular dynamics simulations: a computation board that calculates nonbonded ... section 3 A new formulation for fast calculation of far field force Eq (3) gives solution for outer expansion of P2 M2 Using a similar approach, we obtained solution for inner expansion as: p N...

Ngày tải lên: 22/03/2014, 11:20

... SV40T Ag and the 3¢ portion of the tsA58T Ag cDNA carrying the A4 38V mutation were PCR-amplified from COS-7 cDNAs using the following primers: LTA-1F, 5¢-CTC GAGATGGATAAAGTTTTAAACAGAG-3¢ and LTA1R, ... 5¢-TGAAGGCAAATCTCTGGAC-3¢ for the former, and LTA–M2F, 5¢-CAGCTGTTTTGCTTGAATTATG-3¢ and LTA–2R, 5¢-GAATTCATTATGTTTCAGGTTCA GGGG-3¢ for the latter The PCR products were cloned into the EcoRV site of ... Dong QG, Bernasconi S, Lostaglio S, De Calmanovici RW, Martin-Padura I, Breviario F, Garlanda C, Ramponi S, Mantovani A & Vecchi A (1997) A general strategy for isolation of endothelial cells from...

Ngày tải lên: 18/02/2014, 17:20

... the phase approximation, that is, the phase of (7) arg H e jω , p = −Nω − arg A e jω , p where p is the parameter used to adjust fractional delay and N denotes the order of the designed allpass ... It can be observed that both ετ and εθ of the proposed method are smaller than those of the existing method [23], but the performances of ετ2 and εθ2 for the proposed method are not as good as ... the range of fractional delay For the computational complexity, although the design time of the proposed method is much more than the existing methods, an alternative method has been revealed...

Ngày tải lên: 21/06/2014, 07:20

... Schmidt, A signal subspace approach to multiple emitter location and spectral estimation, Ph.D thesis, Stanford University, Stanford, Calif, USA, 1981 [3] A Paulraj, R Roy, and T Kailath, A subspace ... is based on the original idea which consists in reformulating the estimation problem as a classification problem with two classes An analytical demonstration is provided for a special case of a ... to a high-resolution radar application The goal is to find the most accurate estimate of the range profile of a radar target using its complex signature in the frequency domain An Estimation of...

Ngày tải lên: 23/06/2014, 01:20

... indicates the localization of active laccase in situ Activity of laccase is also stimulated by its natural substrate but the product does not show enough contrast for observation Figs 1-4 show that ... Evolutionarily, laccase and its substrates seem to be an adaptation of the lacquer tree for survival in competition with other life forms, including insects, fungi, humans and herbivorous animals At ... used at 4°C for h Discussion and Conclusion An interesting comparison can be made between histochemical and biochemical methods of enzyme demonstration All laccases previously described catalyze...

Ngày tải lên: 09/08/2014, 03:24

... Contextual word similarity and estimation from sparse data Computer, Speech and Language, 9:123–152 Keiji Shinzato, Tomohide Shibata, Daisuke Kawahara, Chikara Hashimoto, and Sadao Kurohashi 2008 ... relations are used as context profiles as in Kazama and Torisawa (2008) and Kazama et al (2009) From a large corpus of Japanese Web documents (Shinzato et al., 2008) (100 million Scientific Library ... using a large amount of Web data in Japanese and show that the proposed measure gives better word similarities than a non-Bayesian Bhattacharyya coefficient or other well-known similarity measures...

Ngày tải lên: 30/03/2014, 21:20

... crystalline (mostly anatase) and show varied activity depending on the material preparation and annealing atmosphere (Fig 12) Titania nanotubes prepared by the sonoelectrochemical method and annealed ... continuously using a digital multimeter (METEX, MXD The anodized titania nanotubular arrays were annealed in a nitrogen and oxygen atmosphere at 500 ◦ C for h in a CVD furnace at a heating rate of ◦ C/min ... Mohapatra et al / Journal of Catalysis 246 (2007) 362–369 363 466 0A) After an initial increase-decrease transient, the current reached a steady-state value The anodized samples were properly washed...

Ngày tải lên: 05/05/2014, 15:26

... high surface area due to smaller particle and many non-aqueous solvents by adsorbing size and the reactive sites tailored in the form onto a broad range of materials, such as of edge and corner ... decomposition applications of Co-Precipitation in the absence and presence nanosized metal oxides such as AP-MgO, AP- of Polyvinylpyrrolidone (PVP) as a capping CuO, AP-Fe2O3, AP-Al2O3 and AP-CaO [15­ agent ... The average particle size of diffraction angle The average particles size nanoparticles was investigated from line by Debye-Scherrer formula was estimated broadening of the peak at 2θ=0­ 10° via...

Ngày tải lên: 06/05/2014, 08:55

... DCI was performed after the original analysis OSNA runs were repeated from discordant sample homogenates and afterwards RNA was isolated and subjected to qRT-PCR for CK19, CEA, and beta-actin ... levels for each of LN slices) RNA quality was assured by OSNA performed for beta-actin 139 samples gave a negative result and 37 samples gave a positive result with both methods (table 2) No isolated ... M, Nakabayashi K, Yoshidome K, Kaneko T, Iwase T, Akiyama F, Kato Y, Tsuda H, Ueda S, Sato K, Tamaki Y, Noguchi S, et al: One-step nucleic acid amplification for intraoperative detection of lymph...

Ngày tải lên: 18/06/2014, 16:20

... planning and preparation of the manuscript PIF and AES participated in the cutting and staining of Page of the bone tissue RGR participated in the planning of the experiments, data analysis and ... the preparation of the manuscript MJS supervised the study planning, data analysis and preparation of the manuscript All authors read and approved the final manuscript Acknowledgements The authors ... viability of non-treated days cultured bone demonstrated a central area of decreased marrow survival Macroscopically, all treatment groups, cultured on for days, showed almost no detectable marrow...

Ngày tải lên: 20/06/2014, 04:20

... methods for monotone variational inequalities,” Mathematical Programming, vol 104, no 1, pp 39–68, 2005 19 A Bnouhachem, “An LQP method for pseudomonotone variational inequalities,” Journal of ... 1984 25 P N Anh and L D Muu, “Coupling the Banach contraction mapping principle and the proximal point algorithm for solving monotone variational inequalities,” Acta Mathematica Vietnamica, vol 29, ... “Generalized variational inequalities,” Journal of Optimization Theory and Applications, vol 38, no 3, pp 363–383, 1982 20 Journal of Inequalities and Applications 12 C J Goh and X Q Yang, Duality...

Ngày tải lên: 21/06/2014, 07:20

... a more accurate spectral estimation without having to increase the data record length Because of nonstationarity of the respiration signal and more accurate spectral estimation of the spectral ... Extracting the respiration and heartbeat signal Figure 2: The basic flowchart of the signal processing the nonparametric ones, but the advantage of the parametric estimator is that when applicable, ... and the number of samples of the data; (4) perform inverse wavelet transformation to obtain the heartbeat signal EXPERIMENTS AND ANALYSIS There are 15 healthy volunteers who participated in the...

Ngày tải lên: 22/06/2014, 23:20

... The idea of the above construction which improves on products of H3 can be applied to products of other families as well For example, we can better than (1) starting with products of Hk for any ... G.O.H Katona, Extremal Problems for Hypergraphs, Combinatorics, Mathematical Centre Tracts part 56 (1974), 13–42 [4] A. F Sidorenko, The Maximal Number of Edges in a Homogeneous Hypergraph containing ... we may take all the 1-element sets Let Hn be the family of all 1-element sets of a n-set It had been conjectured that the largest cancellative families could be built up by taking products of...

Ngày tải lên: 07/08/2014, 06:20

... points Any AD(m, m) is an a ne plane of order m A classical theorem of Bose states that an a ne plane of order m exists if and only if there exists a complete set of orthogonal frequency squares of ... exists a Hadamard matrix of order 4t Note that Corollary asserts the existence of a type F (2kλ; kλ) complete set without requiring the existence of a Hadamard matrix of order 2kλ (Though of course ... if m and m2 are orders of a ne planes Corollary If there is a Hadamard matrix of order 2k and a complete set of type F (2kλ; λ), then there exists a complete set of type F (2kλ; kλ) Proof This...

Ngày tải lên: 07/08/2014, 06:20

... Avantec Inc., A: An’s Cannula B: Casali’s cannula Fig The new coronary cannula maded for this study (A: An’s Cannula) and the coronary cannula used in the work of Casali et al (B: Casali’s cannula) ... area of aorta cardioplegia cannula placed into aorta Inferior Vena Cava(VCI) and pulmonary vein(PV) localized 10 VCI clamped 11 aorta clamped and 4℃ cardioplegia infused at 300 mmHg simultaneously ... ischemia can be 26 induced regionally Therefore, a new coronary cannulafixed-in-Aorta tube is made to have a multiporous coronary cannula tip to avoid any blockage of coronary branches, providing a...

Ngày tải lên: 07/08/2014, 15:20

... system For example, opportunity can influence motivation as can capability; enacting a behaviour can alter capability, motivation, and opportunity Page of 11 Figure The COM-B system - a framework for ... ways to achieve a behavioural target, whereas the ‘intervention mapping’ approach draws on a range of theoretical approaches each of which independently addresses different aspects of the behaviour ... author information is available at the end of the article specified behaviour patterns In general, these behaviour patterns are measured in terms of the prevalence or incidence of particular behaviours...

Ngày tải lên: 10/08/2014, 10:23

... Yoganandan N, Pintar FA, Kumaresan S, Maiman DJ, Hargarten SW: Dynamic analysis of penetrating trauma J Trauma 1997, 42:266-72 Yetiser S, Kahramanyol M: High-velocity gunshot wounds to the head ... titanate particles appeared in the permanent cavity along the bullet path The cavity was covered with barium titanate particles along the entire bullet path Analysis of the temporary cavity To analyze ... [Characteristics of war wound infection] Acta Med Croatica 2006, 60:353-63 Zhang J, Yoganandan N, Pintar FA, Guan Y, Gennarelli TA: Experimental model for civilian ballistic brain injury biomechanics quantification...

Ngày tải lên: 11/08/2014, 20:21

... bone mass and ortholalveolar form [6] This can be achieved by augmentation of the available substrate using established techniques such as vertical and lateral augmentation of the alveolar ridge, ... definite abutments and removing the auxililary implants final restauration was placed (figure 5) Discussion The main basic criteria for restauration of the edentulous maxilla and mandible are adequate ... implants were accurately positioned in the mandible and the maxilla according to the predefined planning that was made up of DVT scan and a wax up Again bone augmentation around the dental implants...

Ngày tải lên: 11/08/2014, 23:22

... Bland and Altman plots is to assist in the comparison of different assays, thereby defining an optimum assay(s) for a particular analysis Table summarizes the results from Supplementary Data tables ... the PIEF and OGE datasets was analyzed at the protein level for iTRAQ ratios and at the peptide level for pIs ProteinPilot Software v2.0 (an updated and integrated version of ProQuant and ProGroup ... (IMAT) program of the National Cancer Institute, National Institutes of Health, Grant #R33 CA092731 Author details Department of Pathology and Laboratory Medicine, Los Angeles Biomedical Research Institute...

Ngày tải lên: 13/08/2014, 13:20

... inhaled APC can prevent or minimize primary or secondary lung damage when low tidal volumes are used for mechanical ventilation Abbreviations APC, activated protein C; ERK, extracellular-regulated ... injury in anaesthetised sheep Crit Care 2009, 13:R51 Kotanidou A, Loutrari H, Papadomichelakis E, Glynos C, Magkou C, Armaganidis A, Papapetropoulos A, Roussos C, Orfanos SE: Inhaled activated protein ... exposed to large tidal volumes, an important clinical issue Additionally, low-tidal-volume ventilation has become the standard of care in critically ill patients because of studies showing that its...

Ngày tải lên: 13/08/2014, 20:21