a multiform ribosomal protein leader

... database Nucleic Acids Res 2009, 37: D136-140 39 Gama-Castro S, Jimenez-Jacinto V, Peralta-Gil M, Santos-Zavaleta A, Peñaloza-Spindola MI, Contreras-Moreira B, Segura-Salazar J, MuñizRascado ... proteins as well as leader peptides (considered as a particular case of transcriptional attenuation), so as to potentially reannotate some of them A terminator search was carried out using a combination ... 320:1344-1349 Taft RJ, Glazov EA, Cloonan N, Simons C, Stephen S, Faulkner GJ, Lassmann T, Forrest AR, Grimmond SM, Schroder K, Irvine K, Arakawa T, Nakamura M, Kubosaki A, Hayashida K, Kawazu C, Murata M,...

Ngày tải lên: 09/08/2014, 22:23

... LlCBP3 3A was small and ceased after approximately 48 h (Fig 6A) This indicates that LlCBP3 3A only acts on a specic minor subfraction of a- chitin Thus, LlCBP3 3A has an effect on the degradation of a- chitin, ... containing a putative transcription regulator (GenBank ID: AAK06047.1), chitinase gene (GenBank ID: AAK06048.1) and gene encoding a family 33 CBP (GenBank ID: AAK06049.1) was amplied FEBS Journal ... 5Â-AGAGGAGAGTTAG AGCCTTACAAGAAGGGTCCAAAGA-3Â) The PCR product was puried, treated with T4 exonuclease to create vector-compatible overhangs and annealed to a prepared expression vector (pET30 Xa LIC) provided...

Ngày tải lên: 18/02/2014, 08:20

... TCATCAT-3¢; rOMM-64-IV, 5¢-CGCGGATCCGCCCCT GTTAATGATGGAACC-3¢ and 5¢-CGCCTCGAGCTAA GAAGACTGGGCTGCCAG-3¢; rOMM-64-V, 5¢-CGCGG ATCCAGGCAAGATTTTAAGCATCCA-3¢ and 5¢-CGCC TCCACCTAAGAGGCATCCTTGTCCAC-3¢; ... 5¢-CGCCTCCA CCTAAGAGGCATCCTTGTCCAC-3¢; rOMM-64-II, 5¢CGCGGATCCACCGTAGACACTTATGATATA-3¢ and 5¢-CGCCTCGAGCTAAGAGTCAGCTTGCACGTC-3¢; rOMM-64-III, 5¢-CGCGGATCCGCTGATGTGACCAGT GATGAC-3¢ and 5¢-CGCCTCGAGCTATTTGGGCTCTT ... genomic DNA contamination in the total RNA was confirmed by lack of amplification of a b-actin mRNA fragment by PCR using a pair of primers (5¢-ATCACCATCGGCAACGAGAG-3¢ and 5¢-TGGAGTTGTAGGTGGTCTCGTG-3¢)...

Ngày tải lên: 18/02/2014, 17:20

... of GAB catalysis have been identified to date [12] which are based either on a single, bifunctional GAB catalyst or on a GAB catalysts pair The available biochemical data on MshB and LpxC are ... Sorokin A, Anderson I, Galleron N, Candelon B, Kapatral V, Bhattacharyya A, Reznik G, Mikhailova N, Lapidus A et al (2003) Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis ... N-termini are structurally very similar adopting a Rossmann fold motif Although the biological role and the natural substrate of TT1542 remain unknown, MshB has been characterized as a deacetylase...

Ngày tải lên: 19/02/2014, 00:20

... Japan) equipped with U-MNIBA2 and U-MWIG2 mirror units (Olympus), a digital charge-coupled device camera C4742-95–12ER (Hamamatsu Photonics, Hamamatsu, Japan), and aquacosmos 2.0 software (Hamamatsu ... 1962–1965 Abe M, Kobayashi Y, Yamamoto S, Daimon Y, Yamaguchi A, Ikeda Y, Ichinoki H, Notaguchi M, Goto K & Araki T (2005) FD, a bZIP protein mediating signals from the floral pathway integrator FT at ... Hayashida M, Fujii T, Hata Y, Hayashi R & Ueda M (2004) Crystallization and preliminary X-ray analysis of carboxypeptidase Y inhibitor IC complexed with the cognate proteinase Acta Crystallogr D 60, 1622–1624...

Ngày tải lên: 19/02/2014, 05:20

... fusion protein in bacteria A probe for RNAase protection for mouse Cyp4x1 was obtained by PCR of brain RNA with primers 4x1-12-pF, 5¢-CATGGACATAAGTCCTTTTCCCTTCCTCCT-3¢, and 4x1-12-pR, 5¢-AAACATAAATTTCGCCATTTCTCCTAG ... 5¢-AAACATAAATTTCGCCATTTCTCCTAG TAT-3¢ The full-length mouse cDNA was obtained with primers 4x1-f-pF, 5¢-ATGGAGGCCTCCTGGCTGGAG ACTCGTTGG-3¢ and 4x1-f-pR, 5¢-AAACATAAATTT CGCCATTTCTCCTAGTAT-3¢ Total RNA from ... function Experimental procedures Animals and tissue Human cardiovascular system and 12-lane multitissue northern blots, human aorta cDNA library and RACE ready aorta cDNA were obtained from Clontech...

Ngày tải lên: 19/02/2014, 07:20

... Correia IJ, Paquete CM, Coelho A, Almeida CC, Catarino T, Louro RO, Frazao C, Saraiva LM, ˜ Carrondo MA, Turner DL et al (2004) Proton-assisted two-electron transfer in natural variants of tetraheme ... da Silva JJR, Amorim MTS, Cabral MF, Chaves S & Costa J (1991) Dissociation constants of Bronsted acids in D2O and H2O: studies on polyaza and polyoxa–polyaza macrocycles and a general correlation ... interactions by intermolecular nuclear paramagnetic relaxation: plastocyanin from Anabaena variabilis J Am Chem Soc 125, 6858–6859 FEBS Journal 272 (2005) 2251–2260 ª 2005 FEBS C A Salgueiro et al...

Ngày tải lên: 19/02/2014, 17:20

... GCAACATGCCCAAGTTTTATTGTG TATCCTTATCTGTCTGGTCGAGTC AGCATCATGCCCAAGTTTTATTGTGA TTTCTCCCTCCAAAAATATTCAGTTA TCTCCTCTCCTCGAGATGGCGTGTGCTCGCCCACTG ACCGCCGCCTTCTCATCTGA TTCTCTGGAACAACCTTCTCG ATGGCCTCAGTTCCGAAAACCAACAAAATAGA ... ATGGCCTCAGTTCCGAAAACCAACAAAATAGA TTCTGACTTAGAGGCGTTCAGTCATAATCCCA RT-PCR primer, human U1C, sense RT-PCR primer, human U1C, antisense RT-PCR primer, mouse U1C, sense RT-PCR primer, mouse U1C, antisense ... [32P]orthophosphate (Amersham Pharmacia Biosciences, Piscataway, NJ), incubated for 1.5 h and chased with the regular growth medium for h 32P-labeled RNA was isolated and analyzed as described [17] RNA immunoprecipitation...

Ngày tải lên: 20/02/2014, 01:20

... increase intracellular AMP concentration, whereas AICAr is phosphorylated into AICA-ribotide (ZMP), an AMP analogue Both ZMP and AMP are activators of AMPK As already observed in heart [26], AMPK ... and 10 of incubation In contrast, the inactivation of p70S6K by AICAr was slower and was half-maximal only at min, whereas the inactivation of ACC by AICAr was half-maximal at about (Fig 3) The ... activated by the amino-acid-induced accumulation of glutamate and is inhibited by calyculin A [17] Activation of AMPK inactivates ACC by direct phosphorylation of Ser79 An additional inactivation...

Ngày tải lên: 22/02/2014, 07:20

... the mascot search engine [49] for a PMF search in several proprietary and public genomic databases using a tailor-made bioinformatics facility The mascot search was run against all proteins and ... have been reported to act as phytotoxins [e.g cerato-platanin of Ceratocystis fimbriata f sp platani, Snodprot1 of Phaeosphaeria nodorum and Sp1 of Leptosphaeria maculans) or human allergens and ... (Cerato platanin) Ceratocystis fimbriata Q8NJ53 28 Aca1 Antrodia camphorata Q6J935 Botrytis cinerea BC1G_08735 Sp1 (secreted protein1 ) Leptosphaeria maculans Q8J0U4 Snodprot1 Phaeosphaeria nodorum...

Ngày tải lên: 07/03/2014, 12:20

... E GAG A GCC T ACC L TTG A GCC Y TAC E GAG D GAC H CAC D GAC Q CAG A GCA S AGC V GTC S TCC A GCT N AAC K AAG A GCG N AAC E GAG Y TAC D GAC K AAG L CTA K AAG Q CAG L CTC G GGC G GGG E GAG E GAG ... YVSP AA AVG.GAVS.S.K.Q 54 Triticum MQTPKLAILLALAMSAAMANLSQAQNSP Y.SP AA AVG.GAV S.K.Q 54 Zea -MAPRLACLLALAMAAIVVAPCTAQNSP YVDP AA DVG.G.VS.D V 53 Nicotiana MGFVLFSQLPSFLLVSTLL.FLVISHSCRAQNSQ ... CTG K AAG T ACC K AAG P CCC V GTG S AGC T ACC T ACA G GGT L CTC R CGC S TCG M ATG K AAG A GCC K AAG Y TAC I ATT F TTC A GCC T ACA S AGC N AAC V GTC K AAG F TTC S AGC V GTC D GAC L CTC G GGC A GCC...

Ngày tải lên: 07/03/2014, 12:20

... D-Ala-Gly, D-Ala-(Gly)2 (D-Ala)2, D-Ala-L-Ala (D-Ala)3 (D-Ala)4, L-Ala-Gly, L-Ala-(Gly)2 (L-Ala)2, L-Ala-D-Ala, L-Ala-D-Ala-L-Ala, DL-Ala-DL-Asn, DL-Ala-DL-Ile, DL-Ala-DL-Leu, DL-Ala-DL-Met, DL-AlaDL-Phe, ... L-Ala-pNA D-Ala-NH2 L-Ala-NH2 D-Ala-(Gly)2 L-Ala-(Gly)2 b-Ala-L-Ala b-Ala-Gly b-Ala-NH2 b-Ala-L-His (Carnosine) b-Ala-L-Leu (b-Ala)2 similarity to that from dmpA of O anthropi LMG7991 DmpA has ... (BapA) acting on d-Ala-pNA was purified and characterized BapA was found to show a unique substrate specificity for b-alanyl dipeptides H Komeda and Y Asano Results Characterization of the flanking...

Ngày tải lên: 07/03/2014, 21:20

... organisms as diverse as Neurospora crassa, Candida tropicalis, Dictyostelium discoideum, Medicago varia (alfalfa), Arabidopsis thaliana, Oryza sativa (rice), Caenorhabditis elegans, Drosophila ... of protein phosphatase 2A leads to pattern duplication in Drosophila imaginal discs Genes Dev 7, 429±440 Ito, A. , Kataoka, T.R., Watanabe, M., Nishiyama, K., Mazaki, Y., Sabe, H., Kitamura, Y ... domains in Ba and PR72 to the A subunit of PP 2A Fragments of rat Ba and human PR72 encompassing ASBD and were tested for binding to GST and GST -A by incubation for h at 30 °C The precipitated proteins...

Ngày tải lên: 08/03/2014, 10:20

... protein, the cDNA encoding p16 was obtained from the psp54 (28) cDNA The oligonucleotides used as primers were: 5¢-CCCCTCGA GATGTCTAGACAAAAAAAGAGTAG-3¢ and 5¢-CCC CTCGAGTCACACAACAGCACGAC-3¢ The PCR ... [14C]acetyl-CoA in the presence of yeast recombinant Esa1p AUT/PAGE (acetic acid/urea/Triton X-100/PAGE) analysis showed that H3 and H4 are almost fully acetylated, while H 2A contains a mixture of acetylated ... acetylated and nonacetylated isoforms and H2B was not acetylated at all The acetylation and AUT/PAGE analysis of histones was ´ carried out by G Lopez-Rodas, Dept of Biochemistry and Molecular Biology,...

Ngày tải lên: 08/03/2014, 10:20

... Medicaid managed care arrangements depend on contracts appropriate to child health needs and systems Medicaid Managed Care and Child Managed Care Arrangements and Contracts Health To a great extent, ... basis Primary Care Case Management Primary care case management (PCCM) is a form of managed care that can be used to create and sustain a medical home model Participat­ ing families choose a ... org/tabid/631/Default.aspx (Accessed March 19, 2009.) Raphael JL, Guadagnolo BA, Beal AC, and Giardino AP Racial and ethnic disparities in indicators of a primary care medical home for children Acad Pediatr...

Ngày tải lên: 14/03/2014, 09:20

... C-terminal Caskin1 are functional and may interact with SH3 domain-containing proteins, such as Abi2 [We have also found an in vivo association and colocalization of Abi2 with Caskin1 (A Balazs, ... buffer spectrum and averaging 10 separate scans Gel filtration chromatography The unfolded nature of PRD and its fragments was also characterized by gel filtration chromatography The proteins (200 ... proteolysis [5] At typical protease concentrations at which globular proteins are hardly affected, these proteins are degraded rapidly and completely In accordance with this, PRD-His shows a greater sensitivity...

Ngày tải lên: 16/03/2014, 02:20

... NDE15P15 89 (5¢-CCATATGAAGAAAGGAGCAGC-3¢) and Bam-LYS30 reverse (5¢-CGGGATCCTTAATCAATTTCAACTTC ATC-3¢), and the protrialysin cDNA cloned in pGEM-T Easy (Promega, Madison, WI, USA) as template [9] in ... by PCR amplification The template was the same as above, and the reaction included oligonucleotides BAMH15P15 1000 (5¢-CGGATCCGCTGAATATGAACTTG-3¢) and ECOR13LYS (5¢-CGAATTCTTAATCAATTTCAACTTC-3¢) ... megistus saliva (Hemiptera ⁄ Triatominae) Acta Trop 61, 255–261 Amino R, Tanaka AS & Schenkman S (2001) Triapsin, an unusual activatable serine protease from the saliva of the hematophagous vector...

Ngày tải lên: 16/03/2014, 06:20

... PAGE (15% acrylamide) and PhosphorImager analysis To calculate reaction stoichiometries, radiolabeled reaction products and radioactive standards were quantitated using IMAGEQUANT software (Amersham ... (PPM) and phosphoamino acid analysis (PAAA) of mAK-L preparatively phosphorylated by PKC (E) Site-directed mutagenesis analysis of PKC phosphorylation of mAK-L The Coomassie stain and autoradiogram ... quantification purposes Recombinant mAK-S standards have a higher apparent molecular weight than mAK-S in the sample lanes due to N-terminal polyhistidine tags Quantification of relative AK abundance...

Ngày tải lên: 16/03/2014, 18:20

... obtained by RACE, a PCR reaction was performed using a pair of specific primers P3 (5¢-GGAAGAATACAGACACGGACAG-3¢) and P4 (5¢-ATAACAACAGTTTATACATCGCTTC-3¢) corresponding to the 5¢-untranslated and ... was used as template for PCR, which was performed with 1.5 mm MgCl2, 200 lm dNTP, 1.5 U Taq DNA polymerase, and 20 pm of each primer G1 (5¢-ATGGCGGAAGATC TCACAGAAGAACAAA-3¢) and G2 (5¢-TCATTTATTTT ... CaLP2 were used in the nested PCR reactions The first round of PCR reaction was performed with a forward primer UPM (a mixture of primers 5¢-CTAATACGACTCACTATAGGGC AAGCAGTGGTAACAACGCAGAGT-3¢ and...

Ngày tải lên: 16/03/2014, 23:20

... GAATTCAGAGAAAGAGATACGAGATGGA-3¢) and U660033Not (5¢-TCATAAGGCGGCCGCCTACATCG ATCTTAATCTGCTCAA-3¢) A fragment carrying At3g21260 cDNA was amplifed from A thaliana RNA by RT-PCR RNA was isolated ... GLTP1PRONBAM (5¢-CTCCTTGGATCCGCCTGAGAATTGAAAAA GGTGGG-3¢) A 1.3 kb fragment carrying the At1g21360 promoter was amplified using primers 21360PRUXBA (5¢-AACGATCTAGATTAAGAATGTAATCACATTAGG GT-3¢) and 21360PRNNBAM ... activity assays A 1.8 kb DNA fragment carrying the At2g33470 promoter was amplified from the A thaliana Col-0 genome using primers GLTP1PROUXBA (5¢-AGACTGCTCTAGAATG GGTTTCTAAACCAACACGT-3¢) and GLTP1PRONBAM...

Ngày tải lên: 23/03/2014, 07:20