... primers: Aba, 5Â-ATGGACGCTGAAT TCCGTCACGACTCTGGTTACGAAGTTCACCACCAG AAGCTGGTG-3Â;Abb, 5Â-GTTCACCACCAGAAGCT GGTGTTCTTC GCTGAA GACGT GGGTTCT AACAAG GGTGCT-3Â;Abc, 5Â-CACAACGCCACCAACCATCAGA CCGATGATAGCACCCTTGTTAGAACCCAC-3Â;Ab- start, ... 5Â-CACAACGCCACCAACCATCAGA CCGATGATAGCACCCTTGTTAGAACCCAC-3Â;Ab- start, 5Â-GCGTAGGGTCGACATATGGACGCTGAATT CCGTCACG-3Â;Abstop, 5Â-CCTGCCGAGCTCCTATTA CACAACGCCACCAACCATCAG-3Â. The PCR solution was prepared in the buffer supplied with ... enzyme, and contained Aba, Abb and Abcat 40 nm each, and the start and stop primers Abstart and Abstop at 600 nm each, and 200 lm each of dATP, dCTP, dGTP and dTTP. The product was separated from...
Ngày tải lên: 18/02/2014, 13:20
... tsA58T Ag cDNA carry- ing the A4 38V mutation were PCR-amplified from COS-7 cDNAs using the following primers: LTA-1F, 5Â-CTC GAGATGGATAAAGTTTTAAACAGAG-3Â and LTA- 1R, 5Â-TGAAGGCAAATCTCTGGAC-3Â for ... organ-specific blood vascular and lymphatic endothelial cells of the mouse Takashi Yamaguchi, Taeko Ichise, Osamu Iwata, Akiko Hori, Tomomi Adachi, Masaru Nakamura, Nobuaki Yoshida and Hirotake ... LEC, lymphatic endothelial cell; Lyve-1, lymphatic vessel endothelial hyaluronan receptor-1; MACS, magnetic-activated cell separation; MAPK, mitogen-activated protein kinase; PFA, paraformaldehyde;...
Ngày tải lên: 18/02/2014, 17:20
báo cáo hóa học:" A general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open reading frame" pdf
... Transcriptase (M-MLV RT; Promega) and Enhanced Avian Reverse Transcriptase (AMV-RT; Sigma), an enhanced avian myeloblastosis virus reverse tran- scriptase. Reactions were assembled per manufacturer's instructions ... authorized users with valid passwords. The database and all sequence data are backed up to a secure tape- backup system in a different building three times a week. The database will be made available ... made available free of charge to inter- ested parties. Table 7: Amplification efficiency for patients' amplicons Genotype 1a Amplicon A1 A2 A3 A4 x A4 y Amplification efficiency 95 a 98 93...
Ngày tải lên: 20/06/2014, 04:20
Báo cáo hóa học: " Research Article A New Method for Least-Squares and Minimax Group-Delay Error Design of Allpass Variable Fractional-Delay Digital Filters" pdf
... Design of Allpass Variable Fractional-Delay Digital Filters Cheng-Han Chan, 1 Soo-Chang Pei (EURASIP Member), 2 and Jong-Jy Shyu 3 1 Department of Aviation and Communication Electronics, Air Force ... it has been shown that the performance in group delay and phase for the proposed systems can be improved drastically by appropriately specifying the range of fractional delay. For the computational ... satisfactory results. Equation (23) can be further represented in a quadratic form as e k ( a k ) = s τ + a T k Q s a k + a T k Q c a k + r T s a k + r T c a k + a T k Q cs a k + α s b + r T b a k +...
Ngày tải lên: 21/06/2014, 07:20
Báo cáo hóa học: " Research Article A Novel Method for Improving Fairness over Multiaccess Channels" pdf
Ngày tải lên: 21/06/2014, 11:20
Báo cáo hóa học: " A New Method for Estimating the Number of Harmonic Components in Noise with Application in High Resolution Radar" pdf
Ngày tải lên: 23/06/2014, 01:20
Everyone Is a Customer - A Proven Method for Measuring the Value of Every Relationship in the Era of Collaborative Business pdf
Ngày tải lên: 28/06/2014, 22:20
Báo cáo toán học: "A construction method for complete sets of mutually orthogonal frequency squares" pdf
Ngày tải lên: 07/08/2014, 06:20
Báo cáo khoa hoc:" A rapid method for computing the inverse of the gametic covariance matrix between relatives for a marked " pdf
Ngày tải lên: 09/08/2014, 18:21
everyone is a customer a proven method for measuring the value of every relationship in the era of collaborati phần 5 pdf
Ngày tải lên: 10/08/2014, 11:20
Báo cáo y học: "Immobilized pH gradient-driven paper-based IEF: a new method for fractionating complex peptide mixtures before MS analysis" pdf
Ngày tải lên: 13/08/2014, 13:20
Tài liệu Module 7: Creating a Security Design for Accounts pdf
... the account or disable its use. Anyone who can manage a password to an account can, at any time, access all of the information that the account can access. Who can obtain account information. ... primary goals of an attacker is to elevate his privileges to the Administrator or SYSTEM level of access. External and internal attackers may use a variety of approaches to gain access to a network. ... opens a Web page that contains links to each lab. Click a link to begin a lab. Work with a lab partner to perform the lab. ầ To complete a lab 1. Read Ashley Larson’s e-mail in each lab to...
Ngày tải lên: 18/01/2014, 05:20
Tài liệu HOW TO HANDLE DIFFERENT TYPES OF RETAIL SHOPPERS AND MAKE SHOPPING A MEMORABLE EXPERIENCE FOR THEM pdf
... always remembers a store where it got a special treatment. Especially if they had visited this store as a casual visitor. Research indicates the way store staff treats the causal visitors along ... and patient to listen to their knowledgeable views. Therefore instead of you doing the talking, let them speak and as soon as you get a cue that they are aware about the product/brand and are ... store and resource availability. ~ By specifying a target group, a retailer will be able to carve a niche for itself and hence avoid head on competition with big brands/retailers. This eventually...
Ngày tải lên: 19/02/2014, 10:20
Báo cáo khoa học: "A Nonparametric Method for Extraction of Candidate Phrasal Terms" docx
... Annual Meeting of the Association for Computational Linguistics, pages 188-195. Ferreira da Silva, J. and G. Pereira Lopes (1999). A local maxima method and a fair dispersion normalization for ... 605–613, Ann Arbor, June 2005. c 2005 Association for Computational Linguistics A Nonparametric Method for Extraction of Candidate Phrasal Terms Paul Deane Center for Assessment, Design and Scoring ... lexical association measures on unfiltered data no doubt has multiple explanations, but a logical candidate is the failure or inappropriacy of underlying statistical assumptions. For instance,...
Ngày tải lên: 08/03/2014, 04:22
International Labor Migration: A Responsible Role for Business pdf
... Advocacy – Trade unions have advocated the Ministry of Labor for labor law reform and a xed, equal minimum wage for national and foreign workers. » Legal Assistance – Unions help negotiate agreements ... passport » Make passport applications available in villages and towns » Make the passport and visa application process more straightforward 5 ‘‘ A widespread tendency is to regard migrants ... Taskforce is conducting formal national consultations with govern- ments in Brunei Darussalam, Cambodia, Lao PDR, Malaysia and Singapore. » ILO International Migration Program (MIGRANT): Program...
Ngày tải lên: 15/03/2014, 21:20
Food and health in Europe: a new basis for action pdf
... eating patterns that harm public health are not inevitable, but policy-makers need information and data so that they can make informed decisions to prevent harm. Nationally collected data can be ... widespread industrialized animal husbandry are likely to have a greater prevalence of pathogens such as Salmonella and Campylobacter. Croatia (1995–1996) Armenia (1998) Czech Republic (1991) Hungary (1980–1988) Yugoslavia ... discussed. Several issues can already be marked out as requiring action: sustainability and a range of action to protect health. Sustainable and healthy food production Agricultural policies have successfully...
Ngày tải lên: 16/03/2014, 14:20
Báo cáo khoa học: Kinetic hybrid models composed of mechanistic and simplified enzymatic rate laws – a promising method for speeding up the kinetic modelling of complex metabolic networks pptx
... The load charac- teristics were calculated by varying a load parameter within a preset range of physiologically reasonable values. For each value of the load parameter, the steady state was computed ... increased value of k ATPase as compared to the value k 0 ATPase ẳ 1:6h 1 . At values of k ATPase exceeding seven-fold of its normal value, no stationary states can be found; that is, k max ATPase ẳ ... physiologically feasible ranges: 1 2 k 0 ATPase k ATPase 2k 0 ATPase (small variation of the energetic load) 1 5 k 0 ATPase k ATPase 5k 0 ATPase (large variation of the energetic load) 1 50 k 0 ox ...
Ngày tải lên: 23/03/2014, 06:20