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Tài liệu Báo cáo khoa học: A facile method for expression and purification of the Alzheimer’s disease-associated amyloid b-peptide pdf
... primers: Aba, 5¢-ATGGACGCTGAATTCCGTCACGACTCTGGTTACGAAGTTCACCACCAGAAGCTGGTG-3¢;Abb, 5¢-GTTCACCACCAGAAGCTGGTGTTCTTC GCTGAA GACGT GGGTTCT AACAAGGGTGCT-3¢;Abc, 5¢-CACAACGCCACCAACCATCAGACCGATGATAGCACCCTTGTTAGAACCCAC-3¢;Ab-start, ... 5¢-CACAACGCCACCAACCATCAGACCGATGATAGCACCCTTGTTAGAACCCAC-3¢;Ab-start, 5¢-GCGTAGGGTCGACATATGGACGCTGAATTCCGTCACG-3¢;Abstop, 5¢-CCTGCCGAGCTCCTATTACACAACGCCACCAACCATCAG-3¢.The PCR solution was prepared in the buffer suppliedwith ... enzyme, and contained Aba, Abb and Abcat40 nm each, and the start and stop primers Abstart andAbstop at 600 nm each, and 200 lm each of dATP, dCTP,dGTP and dTTP. The product was separated from...
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Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-specific blood vascular and lymphatic endothelial cells of the mouse pdf
... tsA58T Ag cDNA carry-ing the A4 38V mutation were PCR-amplified from COS-7cDNAs using the following primers: LTA-1F, 5¢-CTCGAGATGGATAAAGTTTTAAACAGAG-3¢ and LTA-1R, 5¢-TGAAGGCAAATCTCTGGAC-3¢ for ... organ-specific blood vascular and lymphaticendothelial cells of the mouseTakashi Yamaguchi, Taeko Ichise, Osamu Iwata, Akiko Hori, Tomomi Adachi, Masaru Nakamura,Nobuaki Yoshida and Hirotake ... LEC, lymphatic endothelial cell; Lyve-1, lymphatic vesselendothelial hyaluronan receptor-1; MACS, magnetic-activated cell separation; MAPK, mitogen-activated protein kinase; PFA,paraformaldehyde;...
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Báo cáo khoa học: "A Nonparametric Method for Extraction of Candidate Phrasal Terms" docx
... Annual Meeting of the Association for Computational Linguistics, pages 188-195. Ferreira da Silva, J. and G. Pereira Lopes (1999). A local maxima method and a fair dispersion normalization for ... 605–613,Ann Arbor, June 2005.c2005 Association for Computational Linguistics A Nonparametric Method for Extraction of Candidate Phrasal Terms Paul Deane Center for Assessment, Design and Scoring ... lexical association measures on unfiltered data no doubt has multiple explanations, but a logical candidate is the failure or inappropriacy of underlying statistical assumptions. For instance,...
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Báo cáo khoa học: Kinetic hybrid models composed of mechanistic and simplified enzymatic rate laws – a promising method for speeding up the kinetic modelling of complex metabolic networks pptx
... The load charac-teristics were calculated by varying a load parameterwithin a preset range of physiologically reasonable values. For each value of the load parameter, the steady statewas computed ... increased value of kATPaseas compared to the value k0ATPase¼ 1:6hÀ1. At valuesof kATPaseexceeding seven-fold of its normal value,no stationary states can be found; that is,kmaxATPase¼ ... physiologically feasibleranges:12k0ATPase kATPase 2k0ATPase(small variation of the energetic load)15k0ATPase kATPase 5k0ATPase(large variation of the energetic load)150k0ox...
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USAID Supported a Wide Range of Child and Maternal Health Activities, but Lacked Detailed Spending Data and a Proven Method for Sharing Best Practices pptx
... reports, and program reports, such as the Bureau for Africa’s Child Survival in Sub-Saharan Africa – Taking Stock. We assessed the reliability of financial data compiled and generated by USAID’s ... allocations for child survival across sub-Saharan Africa. USAID officials told us that grantees may pilot innovations (see sidebar) or work in a country’s most rural and hard-to-reach areas. Also, ... the CS/MH account, USAID allocated funds for maternal and child health efforts in 40 countries, in Latin America, sub-Saharan Africa, and South Asia. Figure 2 illustrates the global distribution...
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Báo cáo khoa học: "A Bayesian Method for Robust Estimation of Distributional Similarities" pot
... Estimation of Distributional SimilaritiesJun’ichi Kazama Stijn De Saeger Kow KurodaMasaki Murata†Kentaro TorisawaLanguage Infrastructure Group, MASTAR ProjectNational Institute of Information ... of ACL 94.Ido Dagan, Shaul Marcus, and Shaul Markovitch.1995. Contextual word similarity and estimationfrom sparse data. Computer, Speech and Language,9:123–152.Ido Dagan, Lillian Lee, and ... pro-files are multinomial distributions, the pri-ors are Dirichlet, and the base measure isthe Bhattacharyya coefficient, we can de-rive an analytical form that allows efficientcalculation. For the...
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Báo cáo khoa học: "A Unification Method for Disjunctive Feature Descriptions" pot
... a formula contain informa- tion that is more definite than the information contained in disjunctions. Thus a formula can be regarded as having a definite part, containing only unconditional ... Press, Cam- bridge, England, 1985. [8] Perelra, F. C. N. and D. H. D. Warren. Definite clause grammars for language analysis - a survey of the formal- ism and a comparison with augmented transition ... choosing particular values for the fea- ture). In the analysis of a particular sentence most features have a unique value, and some features are not present at all. When disjunction remains in...
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a novel method for the synthesis of titania nanotubes using
... respectively.2.3. Annealing of the materialsThe anodized titania nanotubular arrays were annealed in a nitrogen and oxygen atmosphere at 500◦Cfor6hinaCVDfur-nace at a heating rate of 1◦C/min. The UAT samples ... the annealed titania nanotubesare crystalline (mostly anatase) and show varied activity de-pending on the material preparation and annealing atmosphere(Fig. 12).Titania nanotubes prepared ... spectra of (a) O2annealed UAT, (b) N2annealed UAT,(c) as-prepared UAT, and (d) H2annealed TiO2nanotubes prepared using eth-ylene glycol and ultrasonic treatment.be due to partial incorporation...
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a novel method for the synthesis of cao nanoparticle
... very faster via using a solvent. The GC chromatograms and area under curve (AUC) data’s are shown in Figures 4 and 5 and Tables 1 and 2. The isopropanol, heptane, toluene and 2-CEPS are diagnosed ... metal atoms. It was reported that polyvinyl pyrrolidone (PVP) could stabilize colloidal particles in water and many non-aqueous solvents by adsorbing onto a broad range of materials, such as ... (PVP) as a capping agent was reported. Then, we have focused our attention on the CaO nanoparticles/Polyvinyl pyrrolidone (PVP) surface as a solid catalyst due to good catalytic properties and...
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Báo cáo hóa học: "One Step Nucleic Acid Amplification (OSNA) - a new method for lymph node staging in colorectal carcinomas" docx
... (DCI)DCI was performed after the original analysis. OSNAruns were repeated from discordant sample homoge-nates and afterwards RNA was isolated and subjectedtoqRT-PCRforCK19,CEA,andbeta-actin.Condi-tions ... Tsujimoto M, Nakabayashi K, Yoshidome K, Kaneko T, Iwase T, Akiyama F,Kato Y, Tsuda H, Ueda S, Sato K, Tamaki Y, Noguchi S, et al: One-stepnucleic acid amplification for intraoperative detection ... inves-tigated with both OSNA (CK19 mRNA as a m arker)and intensive histological methods (H&E and CK19 IHCon 5 levels for each of 2 LN slices). RNA quality wasassured by OSNA performed for beta-actin....
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Báo cáo sinh học: " A general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open reading frame" potx
... times a week.The database will be made available free of charge to inter-ested parties.Table 7: Amplification efficiency for patients' ampliconsGenotype 1a Amplicon A1 A2 A3 A4 x A4 yAmplification ... Transcriptase (M-MLV RT; Promega) andEnhanced Avian Reverse Transcriptase (AMV-RT; Sigma),an enhanced avian myeloblastosis virus reverse tran-scriptase. Reactions were assembled per manufacturer'sinstructions ... remoteaccess, it is secured behind a fire-wall, and access is limitedto authorized users with valid passwords. The databaseand all sequence data are backed up to a secure tape-backup system in a...
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Báo cáo hóa học: " A novel method for neck coordination exercise – a pilot study on persons with chronic non-specific neck pain" docx
... authors would like to thank Nisse Larson for excellent engineering assistance, Maria Frykman for valuable assistance during data collection and Margaretha Marklund for graphical work.References1. ... study,participated in the design, statistical analyses of the studyand helped to draft the manuscript. All authors read andapproved the final manuscript.Additional materialAcknowledgementsThe authors ... self-reported characteristicsSelf-rated pain was assessed as pain at the moment andmeasured within a week before the day of testing on a blank 100 mm visual analogue scale (VAS), on which 0...
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báo cáo hóa học: "A binary method for simple and accurate two-dimensional cursor control from EEG with minimal subject training" potx
... an optimum channel/bin, so we usedBhattacharyya distance plots for real movementFigure 2Bhattacharyya distance plots for real movement. Higher values indicate greater class separability. (a) ... Piccione'ssystem had an average accuracy of 76.2% and a bit rate of7.59 bits/min with healthy trained subjects [15]. Based onthese results, our method appears to have a higher accu-racy at a ... the potential of naturalistic pacing, it still featuressomewhat unnatural control methods (hand, foot, andtongue motor imagery), as well as variable accuracy andhigh computational demand. Thus,...
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báo cáo hóa học:" A general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open reading frame" pdf
... Transcriptase (M-MLV RT; Promega) andEnhanced Avian Reverse Transcriptase (AMV-RT; Sigma),an enhanced avian myeloblastosis virus reverse tran-scriptase. Reactions were assembled per manufacturer'sinstructions ... authorized users with valid passwords. The databaseand all sequence data are backed up to a secure tape-backup system in a different building three times a week.The database will be made available ... made available free of charge to inter-ested parties.Table 7: Amplification efficiency for patients' ampliconsGenotype 1a Amplicon A1 A2 A3 A4 x A4 yAmplification efficiency 95 a 98 93...
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