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Tài liệu Báo cáo khoa học: A facile method for expression and purification of the Alzheimer’s disease-associated amyloid b-peptide pdf
Ngày tải lên :
18/02/2014, 13:20
... primers: Aba, 5¢-ATGGACGCTGAAT
TCCGTCACGACTCTGGTTACGAAGTTCACCACCAG
AAGCTGGTG-3¢;Abb, 5¢-GTTCACCACCAGAAGCT
GGTGTTCTTC GCTGAA GACGT GGGTTCT AACAAG
GGTGCT-3¢;Abc, 5¢-CACAACGCCACCAACCATCAGA
CCGATGATAGCACCCTTGTTAGAACCCAC-3¢;Ab-
start, ... 5¢-CACAACGCCACCAACCATCAGA
CCGATGATAGCACCCTTGTTAGAACCCAC-3¢;Ab-
start, 5¢-GCGTAGGGTCGACATATGGACGCTGAATT
CCGTCACG-3¢;Abstop, 5¢-CCTGCCGAGCTCCTATTA
CACAACGCCACCAACCATCAG-3¢.
The PCR solution was prepared in the buffer supplied
with ... enzyme, and contained Aba, Abb and Abcat
40 nm each, and the start and stop primers Abstart and
Abstop at 600 nm each, and 200 lm each of dATP, dCTP,
dGTP and dTTP. The product was separated from...
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Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-specific blood vascular and lymphatic endothelial cells of the mouse pdf
Ngày tải lên :
18/02/2014, 17:20
... tsA58T Ag cDNA carry-
ing the A4 38V mutation were PCR-amplified from COS-7
cDNAs using the following primers: LTA-1F, 5¢-CTC
GAGATGGATAAAGTTTTAAACAGAG-3¢ and LTA-
1R, 5¢-TGAAGGCAAATCTCTGGAC-3¢ for ... organ-specific blood vascular and lymphatic
endothelial cells of the mouse
Takashi Yamaguchi, Taeko Ichise, Osamu Iwata, Akiko Hori, Tomomi Adachi, Masaru Nakamura,
Nobuaki Yoshida and Hirotake ... LEC, lymphatic endothelial cell; Lyve-1, lymphatic vessel
endothelial hyaluronan receptor-1; MACS, magnetic-activated cell separation; MAPK, mitogen-activated protein kinase; PFA,
paraformaldehyde;...
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Báo cáo khoa học: "A Nonparametric Method for Extraction of Candidate Phrasal Terms" docx
Ngày tải lên :
08/03/2014, 04:22
... Annual Meeting
of the Association for Computational Linguistics,
pages 188-195.
Ferreira da Silva, J. and G. Pereira Lopes (1999). A
local maxima method and a fair dispersion
normalization for ... 605–613,
Ann Arbor, June 2005.
c
2005 Association for Computational Linguistics
A Nonparametric Method for Extraction of Candidate Phrasal Terms
Paul Deane
Center for Assessment, Design and Scoring ... lexical
association measures on unfiltered data no doubt
has multiple explanations, but a logical candidate
is the failure or inappropriacy of underlying
statistical assumptions. For instance,...
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Báo cáo khoa học: Kinetic hybrid models composed of mechanistic and simplified enzymatic rate laws – a promising method for speeding up the kinetic modelling of complex metabolic networks pptx
Ngày tải lên :
23/03/2014, 06:20
... The load charac-
teristics were calculated by varying a load parameter
within a preset range of physiologically reasonable values.
For each value of the load parameter, the steady state
was computed ... increased value of k
ATPase
as compared to the value k
0
ATPase
¼ 1:6h
À1
. At values
of k
ATPase
exceeding seven-fold of its normal value,
no stationary states can be found; that is,
k
max
ATPase
¼ ... physiologically feasible
ranges:
1
2
k
0
ATPase
k
ATPase
2k
0
ATPase
(small variation of the energetic load)
1
5
k
0
ATPase
k
ATPase
5k
0
ATPase
(large variation of the energetic load)
1
50
k
0
ox
...
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USAID Supported a Wide Range of Child and Maternal Health Activities, but Lacked Detailed Spending Data and a Proven Method for Sharing Best Practices pptx
Ngày tải lên :
28/03/2014, 09:20
... reports, and program
reports, such as the Bureau for Africa’s Child Survival in Sub-Saharan
Africa – Taking Stock.
We assessed the reliability of financial data compiled and generated by
USAID’s ... allocations for child survival
across sub-Saharan Africa.
USAID officials told us that grantees may pilot innovations (see sidebar)
or work in a country’s most rural and hard-to-reach areas. Also, ... the CS/MH account, USAID
allocated funds for maternal and child health efforts in 40 countries, in
Latin America, sub-Saharan Africa, and South Asia. Figure 2 illustrates the
global distribution...
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Báo cáo khoa học: "A Bayesian Method for Robust Estimation of Distributional Similarities" pot
Ngày tải lên :
30/03/2014, 21:20
... Estimation of Distributional Similarities
Jun’ichi Kazama Stijn De Saeger Kow Kuroda
Masaki Murata
†
Kentaro Torisawa
Language Infrastructure Group, MASTAR Project
National Institute of Information ... of ACL 94.
Ido Dagan, Shaul Marcus, and Shaul Markovitch.
1995. Contextual word similarity and estimation
from sparse data. Computer, Speech and Language,
9:123–152.
Ido Dagan, Lillian Lee, and ... pro-
files are multinomial distributions, the pri-
ors are Dirichlet, and the base measure is
the Bhattacharyya coefficient, we can de-
rive an analytical form that allows efficient
calculation. For the...
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Báo cáo khoa học: "A Unification Method for Disjunctive Feature Descriptions" pot
Ngày tải lên :
31/03/2014, 17:20
... a formula contain informa-
tion that is more definite than the information contained in
disjunctions. Thus a formula can be regarded as having a
definite part, containing only unconditional ... Press, Cam-
bridge, England, 1985.
[8] Perelra, F. C. N. and D. H. D. Warren. Definite clause
grammars for language analysis - a survey of the formal-
ism and a comparison with augmented transition ... choosing particular values for the fea-
ture). In the analysis of a particular sentence most features
have a unique value, and some features are not present at all.
When disjunction remains in...
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a novel method for the synthesis of titania nanotubes using
Ngày tải lên :
05/05/2014, 15:26
... respectively.
2.3. Annealing of the materials
The anodized titania nanotubular arrays were annealed in a
nitrogen and oxygen atmosphere at 500
◦
Cfor6hinaCVDfur-
nace at a heating rate of 1
◦
C/min. The UAT samples ... the annealed titania nanotubes
are crystalline (mostly anatase) and show varied activity de-
pending on the material preparation and annealing atmosphere
(Fig. 12).
Titania nanotubes prepared ... spectra of (a) O
2
annealed UAT, (b) N
2
annealed UAT,
(c) as-prepared UAT, and (d) H
2
annealed TiO
2
nanotubes prepared using eth-
ylene glycol and ultrasonic treatment.
be due to partial incorporation...
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a novel method for the synthesis of cao nanoparticle
Ngày tải lên :
06/05/2014, 08:55
... very faster
via using a solvent. The GC chromatograms
and area under curve (AUC) data’s are shown
in Figures 4 and 5 and Tables 1 and 2. The
isopropanol, heptane, toluene and 2-CEPS
are diagnosed ... metal atoms. It was
reported that polyvinyl pyrrolidone (PVP)
could stabilize colloidal particles in water
and many non-aqueous solvents by adsorbing
onto a broad range of materials, such as ... (PVP) as a capping
agent was reported. Then, we have focused
our attention on the CaO nanoparticles/
Polyvinyl pyrrolidone (PVP) surface as a solid
catalyst due to good catalytic properties and...
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Báo cáo hóa học: "One Step Nucleic Acid Amplification (OSNA) - a new method for lymph node staging in colorectal carcinomas" docx
Ngày tải lên :
18/06/2014, 16:20
... (DCI)
DCI was performed after the original analysis. OSNA
runs were repeated from discordant sample homoge-
nates and afterwards RNA was isolated and subjected
toqRT-PCRforCK19,CEA,andbeta-actin.Condi-
tions ... Tsujimoto M, Nakabayashi K, Yoshidome K, Kaneko T, Iwase T, Akiyama F,
Kato Y, Tsuda H, Ueda S, Sato K, Tamaki Y, Noguchi S, et al: One-step
nucleic acid amplification for intraoperative detection ... inves-
tigated with both OSNA (CK19 mRNA as a m arker)
and intensive histological methods (H&E and CK19 IHC
on 5 levels for each of 2 LN slices). RNA quality was
assured by OSNA performed for beta-actin....
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Báo cáo sinh học: " A general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open reading frame" potx
Ngày tải lên :
19/06/2014, 08:20
... times a week.
The database will be made available free of charge to inter-
ested parties.
Table 7: Amplification efficiency for patients' amplicons
Genotype 1a
Amplicon A1 A2 A3 A4 x A4 y
Amplification ... Transcriptase (M-MLV RT; Promega) and
Enhanced Avian Reverse Transcriptase (AMV-RT; Sigma),
an enhanced avian myeloblastosis virus reverse tran-
scriptase. Reactions were assembled per manufacturer's
instructions ... remote
access, it is secured behind a fire-wall, and access is limited
to authorized users with valid passwords. The database
and all sequence data are backed up to a secure tape-
backup system in a...
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Báo cáo hóa học: " A novel method for neck coordination exercise – a pilot study on persons with chronic non-specific neck pain" docx
Ngày tải lên :
19/06/2014, 08:20
... authors would like to thank Nisse Larson for excellent engineering
assistance, Maria Frykman for valuable assistance during data collection and
Margaretha Marklund for graphical work.
References
1. ... study,
participated in the design, statistical analyses of the study
and helped to draft the manuscript. All authors read and
approved the final manuscript.
Additional material
Acknowledgements
The authors ... self-reported characteristics
Self-rated pain was assessed as pain at the moment and
measured within a week before the day of testing on a
blank 100 mm visual analogue scale (VAS), on which 0...
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báo cáo hóa học: "A binary method for simple and accurate two-dimensional cursor control from EEG with minimal subject training" potx
Ngày tải lên :
19/06/2014, 08:20
... an optimum channel/bin, so we used
Bhattacharyya distance plots for real movementFigure 2
Bhattacharyya distance plots for real movement. Higher values indicate greater class separability. (a) ... Piccione's
system had an average accuracy of 76.2% and a bit rate of
7.59 bits/min with healthy trained subjects [15]. Based on
these results, our method appears to have a higher accu-
racy at a ... the potential of naturalistic pacing, it still features
somewhat unnatural control methods (hand, foot, and
tongue motor imagery), as well as variable accuracy and
high computational demand. Thus,...
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báo cáo hóa học:" A general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open reading frame" pdf
Ngày tải lên :
20/06/2014, 04:20
... Transcriptase (M-MLV RT; Promega) and
Enhanced Avian Reverse Transcriptase (AMV-RT; Sigma),
an enhanced avian myeloblastosis virus reverse tran-
scriptase. Reactions were assembled per manufacturer's
instructions ... authorized users with valid passwords. The database
and all sequence data are backed up to a secure tape-
backup system in a different building three times a week.
The database will be made available ... made available free of charge to inter-
ested parties.
Table 7: Amplification efficiency for patients' amplicons
Genotype 1a
Amplicon A1 A2 A3 A4 x A4 y
Amplification efficiency 95
a
98 93...
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