a general synthesis of macrocyclic ruthenium ii metal complexes

... Birmingham and Basel has given great support for my research work Thanks for the help and friendship from all my colleagues: Tao, Pan, Valerie J, Ayten, Robyn, Azad, Chris, Annette, Amar, Barbara, ... number and orientation of the coordination sites of ligands [molecular components containing metal- binding domains] and (2) the coordination number and geometry of the metal ions A variety of metallosupramolecular ... chemistry Metallosupramolecular chemistry has become a main research area in supramolecular chemistry Constable has defined metallosupramolecular chemistry as follows: "Metallosupramolecular chemistry...

Ngày tải lên: 02/07/2014, 13:33

... a- amino-b-carboxymuconate-e-semialdehyde decarboxylase J Am Chem Soc 129, 9278–9279 Fukuoka SI, Ishiguro K, Yanagihara K, Tanabe A, Egashira Y, Sanada H & Shibata K (2002) Identification and expression of a cDNA encoding human 6622 ... a- amino-b-carboxymuconate-e-semialdehyde decarboxylase (ACMSD) J Biol Chem 277, 35162–35167 Tanabe A, Egashira Y, Fukuoka SI, Shibata K & Sanada H (2002) Expression of rat hepatic 2-amino3-carboxymuconate-6-semialdehyde ... recently, abnormally high brain levels of PA have been reported in a murine model of cerebral malaria, a frequently fatal complication of Plasmodium falciparum infection; in the same model, pharmacological...

Ngày tải lên: 18/02/2014, 06:20

... were amplified by PCR using pairs of primers 5¢-CATATGCAACAGACAACCCAAATTCAGCCC-3¢ and 5¢-AGATCTTATCACTCCCTTACTAAGTCGATG-3¢ for the b subunit and 5¢-CATATGAGCGAGAAAACCA TGCGCGTGCAG-3¢ and 5¢-AGATCTTAGCTTCCTTT ... DNA polymerase (Stratagene) and pairs of primers 5¢-TCTGAGTGCGGTGGAAGAGATG ATGAAGCG-3¢ and 5¢-AGATCTTATTCAATGGTGT CGGGCTGAACC-3¢ and digested with EcoRV and BglII Resulting 210-bp fragment was ... pET19b(H6bG) A pair of synthetic oligonucleotides, 5¢-TATGGGCAGCAGCCATCATCATCATCATCACA GCAGCGGCCTGGTGCCGCGCGGCAGCAC-3¢ and 5¢-TAGTGCTGCCGCGCGGCACCAGGCCGCTGCT GTGATGATGATGATGATGGCTGCTGCCCA-3¢ were...

Ngày tải lên: 21/02/2014, 03:20

... The DASA of the AB dimer is defined as [(ASA of A) + (ASA of B) ) (ASA of AB dimer)] ⁄ The number of water molecules in the dimer interface was detected with ASV CALCULATOR [34] ASA was calculated ... was calculated (Table 1) It is conceivable that a dimer with larger DASA is more plausible We found that the DASAs of the AB and DA dimers were comparable Although the DASA of the CD dimer was ... beamline staff of PF and SPring-8 for data collection We also acknowledge the kind support of M Maeda (National Institute of Agrobiological Sciences, Japan) for calculation of DASA This work was...

Ngày tải lên: 14/03/2014, 23:20

... precipitant Prior to data collection, a crystal was transferred into Analysis of substrate specificity Substrate specificity was analyzed using xyloglucan tetradecasaccharide, XXXGXXXG, prepared as ... at various concentrations of the substrate, tamarind seed xyloglucan (Dainippon Sumitomo Pharma, Osaka, Japan), in 50 mm sodium acetate buffer (pH 5.5) at 45 °C for 15 The bicinchoninate assay ... 760–763 18 Navaza J (1994) AMoRe: an automated package for molecular replacement Acta Crystallogr A 50, 157–163 19 Brunger AT, Adams PD, Clore GM, DeLano WL, ¨ Gros P, Grosse-Kunstleve RW, Jiang JS,...

Ngày tải lên: 23/03/2014, 05:22

... Glu272–Arg148 21 19 14 092 8248 5844 –1 6.5 No of hydrogen bonds (side chain–side chain) Aromatic stacking ˚ ASA total (A2 ) ˚ ASA apolar (A2 ) ˚ ASA polar (A2 ) Formal global charge Theoretical pIb ... and aromatic interactions are similar in all enzymes Frequently, alterations of the accessible surface of nonpolar side chains and of the accessible charged surfaces are observed in cold-adapted ... activity: crystallographic structure at 2 -A resolution of cephalosporinase from the ampC gene of Enterobacter cloacae P99 and comparison with a class A penicillinase Proc Natl Acad Sci USA 90, 11257–11261...

Ngày tải lên: 23/03/2014, 07:20

... in the crystal and no part of it has got cleaved off during the crystallization process 4.5.2 Data collection and analysis The native and selenomethionylated crystal data sets for all the five ... But a few of these crystals from the condition containing PEG 6000 and t-butanol diffracted up to about Å 4.3.2 Data collection and analysis Data collection was done at the X25 beamline, National ... oscillation one degree for the respective crystals The crystal parameters and data collection statistics are given in Table below Table Crystal parameters and data-collection statistics for wt AtCyP38...

Ngày tải lên: 14/09/2015, 17:50

... Table 2.1 40 Figure 2.5 lAmyA crystal picture Crystals of AmyA, with maximum dimensions of 0.1 x 0.1 x 0.6 mm Table 2.1 Crystal parameters and data collection statistics of AmyA at low salt Values ... report, partially purified AmyA that contained the signal peptide with a His-tag was used for the activity assay From the crystal structure of AmyA it appears that the His-tag and Nterminal signal ... temperature above 60 °C makes AmyA a true halo-thermophilic enzyme 2.1.4 The α-amylases: Alpha-Amylases (alpha-1,4-glucan-4-glucanohydrolase, EC 3.2.1.1) are Endo-acting enzymes that catalyze...

Ngày tải lên: 16/09/2015, 08:31

... in the most favourable region of the Ramachandran plot (Table 2) Crystal structure of a Thielavia arenaria laccase Kinetic data Kinetic constants (Km and Vmax) for rMaL, TaLcc1 and ThL were determined ... structure of a metallo-oxidase Fet3p Proc Natl Acad Sci USA 102, 15459–15464 Smith AW, Camara-Artigas A, Wang M, Allen JP & Francisco WA (2006) Structure of phenoxazinone synthase from Streptomyces antibioticus ... Crystal structure of a Thielavia arenaria laccase J P Kallio et al 15 Lyashenko AV, Zhukova Y, Zhukhlistova N, Zaitsev V, Stepanova E, Kachalova G, Koroleva O, Voelter W, Betzel C, Tishkov V et al...

Ngày tải lên: 14/02/2014, 18:20

... molecules and peptide atoms) and rj is the standard deviation of B factors The normalized B factors have a zero mean and unit variance All atoms that satisfy Bz ‡ are treated as outliers and discarded ... supernatant was added to a 3-mL solution of either nickel nitrilotriacetic acid agarose resin (Qiagen, Valencia, CA, USA) or Profinity immobilized metal affinity chromatography (IMAC) resin (Bio-Rad, ... with a contact area ˚ of 13.5 A2 and the Lys residue at P3 with a contact ˚ area of 10.2 A2 Residues outside the central binding area have significantly lower values These results are summarized...

Ngày tải lên: 14/02/2014, 19:20

... Academia Sinica and National Synchrotron Radiation Research Center (Taiwan, ROC) We thank Ms M.Sakai (Osaka University) for performing ultracentrifugation analysis and Mr K Mieda and M Sakata ... hyperthermophilic archaeon growing at temperatures up to 100°C Int J Syst Bacteriol 46, 1070–1077 10 Kawarabayasi Y, Hino Y, Horikawa H, Yamazaki S, Haikawa Y, Jin-no K, Takahashi M, Sekine M, Baba S, Ankai A ... coordination around the metal cofactor The absence of an anomalous Fourier map demonstrated that the active site in apo-ApeSOD did not contain a metal cofactor (Fig 3C) However, the side chains and...

Ngày tải lên: 14/02/2014, 22:20

... conserved active-site motifs, RHGXRXP and HD, and hydrolyze metal- free phytate with pH optima in the acidic range They consist of two domains, a large a ⁄ b domain and a small a domain with the catalytic ... values increase from G1P over PhyK to AppA by a factor of  2200 The conformational changes of AppA upon substrate binding facilitate a faster turnover of phytate and are in line with a higher specificity ... TGGCAGCTG) and KlebTH-rv (5¢-CCGGCGGTAGC CATGGTCCTGCCGAAGCTT) and chromosomal DNA of Klebsiella strain ASR1 as a template The PCR product was cloned into the BglII and HindIII sites of plasmid pET22b(+)...

Ngày tải lên: 16/02/2014, 09:20

... Val-Ser-Ala-ArgflMet-Ala-Pro and Phe-Thr-Phe-ArgflSer-Ala-Arg for PAI-1 and PCI, respectively [6] By contrast, the reactive site loops of a1 -antichymotrypsin and heparin cofactor II contain leucine instead of arginine ... favored and favored regions of the Ramachandran ˚ plot and r.m.s.d values for bond and angle of 0.005 A and 1.37 ° as shown in Table References Table Data collection and refinement statistics of ... Shimomura T, Denda K, Kitamura A, Kawaguchi T, Kito M, Kondo J, Kagaya S, Qin L, Takata H, Miyazawa K et al (1997) Hepatocyte growth factor activator inhibitor, a novel Kunitz-type serine protease...

Ngày tải lên: 19/02/2014, 00:20

... types of GAB catalysis have been identified to date [12] which are based either on a single, bifunctional GAB catalyst or on a GAB catalysts pair The available biochemical data on MshB and LpxC are ... families database Nucleic Acids Res 30, 276–280 Handa N, Terada T, Kamewari Y, Hamana H, Tame JRH, Park S-Y, Kinoshita K, Ota M, Nakamura H, Kuramitsu S et al (2003) Crystal structure of the conserved ... biosynthetic pathway of mycothiol [10]; its catalytic residues are similar to those found in metalloproteases so that a catalytic mechanism similar to that of metalloproteases [8,11,12] has been proposed...

Ngày tải lên: 19/02/2014, 00:20

... atoms of Asp11 and Asn23 (Fig 3) in an arrangement similar to what is observed in VPRK Both PRK and VPRK have calcium bound at Ca3 SPRK also has an aspartic acid residue at position at 200, and ... initial comparative studies showed that the catalytic turnover was at least twice that of PRK, but substrate affinity was reduced SPRK was compared with PRK and was found to be remarkable stable against ... hexa62 Table Data collection and refinement statistics for SPRK ˚ Resolution (A) Space group Cell parameters ˚ a- axis (A) ˚ b-axis (A) ˚ c-axis (A) b angle (°) Number of observations (24 – maximum...

Ngày tải lên: 19/02/2014, 07:20

... hydrogen bonds Main chain–main chain Main chain–side chain Side chain–side chain Total ˚ Exposed surface areab (A2 ) ˚ Apolarc (A2 ) ˚ Buried surface areab (A2 ) ˚ Apolarc (A2 ) a 1IC6 1THM 38 24 ⁄ ... Structural aspects of cold adaptation surface with their hydrophilic nature may enhance favourable electrostatic interaction with water at low temperature and, at the same time, result in an anionic ... whereas the overall exposed surface areas of the psychro- and the mesophilic enzymes are larger than for the thermophile enzyme, mainly as a result of larger area of apolar atoms, the meso- and...

Ngày tải lên: 19/02/2014, 16:20

... oligonucleotides: a sense primer 5¢-CAACAAATTTGCATATGACTATT AAAG-3¢ containing an NdeI site (underlined) next to the start codon of the T brucei gGAPDH gene; an antisense primer 5¢-CAGCCAAGCGCCTAGGGAGCGAGA AC-3¢, ... Castellano, E.E., Garratt, R.C & Craivich, A (1998) Set-up and experimental parameters of the protein crystallography beamline at the Brazilian National Synchrotron Laboratory J Synchr Rad 5, ... modes of these 1,3-BPGA analogues to the two trypanosomatid enzymes Materials and methods Sources of substrates, cofactors and inhibitors The synthesis of 1,3-BPGA analogues used in this study has...

Ngày tải lên: 20/02/2014, 02:21

... simulation programme for displaying reciprocal-space diffraction data J Appl Crystallogr 32, 375–376 Navaza, J (1994) AMoRe: an automated package for molecular replacement Acta Crystallogr A5 0, ... S., Marion-Poll, A. , Caboche, M., Kamiya, Y & Koshiba, T (1998) Molecular cloning and characterization of aldehyde oxidases in Arabidopsis thaliana Plant Cell Physiol 39, 433–442 Koga, J., Adachi, ... intermediate in enzymatic thiamin catalysis: Crystal structure of the a- carbanion of (a, b-dihydroxyethyl) -thiamin diphosphate in the active site of transketolase from Saccharomyces cerevisiae Proc Natl...

Ngày tải lên: 20/02/2014, 11:20

... residues was minimal (451456 aa; six aa in total) A search for structural homologues of the C-terminal domain using the DALI [15] databases identified SpoIIAA (anti-anti-sigma factor; PDB 1til, chain ... 765–773 Wakayama M, Yamagata T, Kamekura A, Boontim N, Yano S, Tachiki T, Yoshimune K & Moriguchi M (2005) Characterization of salt-tolerant glutaminase from Stenotrophomonas maltophila NYW-81 and ... et al Crystal structure of salt-tolerant glutaminase A B Fig L-Glutamate in complex with Mglu and asparaginase of Erwinia carotovora LGlutamate (cylinder) on the surfaces of the structures of...

Ngày tải lên: 06/03/2014, 09:22

... aaagtgaagtcc-3¢ and 5¢-attattggatccctattgctggtccgattctgccag-3¢ 3-TPR NlpI primers (residues 62–197) were 5¢-aataatccatgg gggcacagcttttatatgagcgcggag-3¢ and 5¢-aataatggatcctcactgttc cttatccgatttttcgaagtgc-3¢ ... chemically synthesized by the W M Keck Core Facility (Yale University, New Haven, CT, USA) Primers to amplify mature NlpI (residues 20–294) were 5¢-aataatccatggggagtaatacttcctggcgta aaagtgaagtcc-3¢ ... (Finland) Data for this study were measured at beamline X12C of the National Synchrotron Light Source We are grateful for the assistance of Dr Anand Saxena in the use of this beamline The National...

Ngày tải lên: 07/03/2014, 16:20