a brief introduction to the current status

... originally a wild plant, is native to the Dalmatian region of the former Yugoslavia and Persia It is classified taxonomically into the following three species [2]: Chrysanthemum cinerariaefolium (Tanacetum ... Japan n.matsuo@kincho.co.jp Health & Crop Sciences Research Laboratory Sumitomo Chemical Co., Ltd 4-2-1 Takatsukasa Takarazuka Hyogo 665-8555 Japan morit7@sc.sumitomo-chem.co.jp Editorial Board ... useful to the reader The coverage is not exhaustive in data, but rather conceptual, concentrating on the methodological thinking that will allow the nonspecialist reader to understand the information...

Ngày tải lên: 29/05/2014, 23:59

... 5¢-CCAGC GTCCACCAACTTATTAC-3¢; murine ADAM9 forward, 5¢-CTTAACATCCCGAAGCCTGAC-3¢; murine ADAM9 reverse, 5¢-CTCACTGGTCTTCCCTCTGC-3¢; human ADAM10 forward, 5¢-TTCAGGAAGCTCTGGAGGA A- 3¢; human ADAM10 ... 5¢-AGCACTGTGTTGGCGTACAG-3¢; murine b-actin forward, 5¢-AGCCATGTACGTAGCCATCC-3¢; murine b-actin reverse, 5¢-CTCTCAGCTGTGGTGGTG AA-3¢; human ADAM9 forward, 5¢-GAATGAATCACG ATGATGGGAG-3¢; human ADAM9 reverse, ... cellular membrane, whereas ADAM17 translocation to the cytoplasm was seen after h of treatment, and continually increased over the 24 h of testing ADAM17 ADAM9 µM ATRA (h) ADAM10 looking particularly...

Ngày tải lên: 16/03/2014, 01:20

... regulates polarized Numb-mediated endocytosis for axon growth Nat Cell Biol 5, 819–826 10 Arimura N, Menager C, Kawano Y, Yoshimura T, Kawabata S, Hattori A, Fukata Y, Amano M, Goshima Y, Inagaki ... regulated at the transcriptional level gives us another example of a coordinated cell response at both the mRNA synthesis and protein activation stages Experimental procedures Materials ATRA and ActD ... boric acid and mm EDTA, pH 8.3) After the electrophoresis, the gel was dried, autoradiographed, and analyzed using imagemaster software (Amersham Biosciences) RT-PCR analysis Total RNA (2 lg) was...

Ngày tải lên: 16/03/2014, 12:20

... for the dilution heat (A) , and plotted against the molar ratio (GA ⁄ c-CD) The data were fitted using a nonlinear least-squares method Table Thermodynamic parameters of the interaction between GA ... glucuronic acid and c-CD (data not shown), the aglycone portion of GA should penetrate into the hydrophobic pocket of c-CD To determine the thermodynamic parameters, the area of each exothermic peak as ... least-squares method (Fig 2E) The thermodynamic parameters at 25 °C are summarized in Table Similar thermodynamic parameters at different pH values between 4.5 and 9.5 indicate that the chemical structures...

Ngày tải lên: 16/03/2014, 14:20

... Smirnov, A. V., Kulakovskaya, T.V & Kulaev, I.S (2002) Exopolyphosphatase of the halotolerant bacterium Brevibacterium sp.strain VKM Ac-2118 grown at normal and enhanced salinity Doklady Acad Nauk ... corresponding carbon atom resonates at d 71.1) In turn, there was a correlation between the latter proton and the proton of a –CHOP– group at d 4.37 (the corresponding carbon atom resonates at d 75.3) ... teichoic acid Acid degradation of the trichloroacetic acid extract (crude preparation) yielded mannitol phosphates and glycerol phosphates Therefore, it was necessary to establish whether these...

Ngày tải lên: 23/03/2014, 15:21

... and Rac2 isoforms in the membrane The plasma membrane fraction was separated from neutrophils and HL-60 cells were harvested at 0, 1, 3, and days after ATRA treatment Rac-PAK binding assay was ... clear in the plasma membrane fraction from either the undifferentiated cells or mature neutrophils (Fig 4B) These data suggest that Rac and PAK located in the membrane may interact to play a role ... ATRA for a week, and Rac and PAK were assayed in both cytosol and membrane fractions Rac occurs as two isoforms (Rac1 and Rac2) that are 92% identical in amino-acid sequence and Rac2 is more abundantly...

Ngày tải lên: 31/03/2014, 15:20

... mitochondrial depolarization, which resulted in caspase-3 associated apoptotic cell death Thus, Aurora kinase inhibitor VX-680 offered as a novel therapeutic agent in treatment of ATRA-resistant ... an interaction of a number of key cellular regulatory pathways, including cell proliferation pathway, cell survival pathway, caspase activation pathway, tumor suppressor pathway, death receptor ... pathway, mitochondrial pathway and protein kinase pathway Most cells apoptosis pathway is through mitochondrial-mediated pathway, which is mostly regulated by Bcl-2 family, including the antiapoptotic...

Ngày tải lên: 18/06/2014, 19:20

... in the lumbar spinal cord [19] When ATRA was administered intrathecally, the sensitization-like effect was inhibited by a RARpan-antagonist and associated with a modulation of COX-2 and IL-1 activities ... [21] Materials and methods Drugs and other reagents The RARs pan-antagonist ATRA (all trans-retinoic acid) was purchased from Sigma (St Louis, MO) The selective RAR pan-antagonist (LE540) and RXR ... using a commercially available enzyme immunoabsorbent assay (EIA) kit (Cayman Chemical Company, Ann Arbor, MI) following the manufacturer's protocol The assay was performed in a total volume of...

Ngày tải lên: 19/06/2014, 22:20

... mitochondrial depolarization, which resulted in caspase-3 associated apoptotic cell death Thus, Aurora kinase inhibitor VX-680 offered as a novel therapeutic agent in treatment of ATRA-resistant ... an interaction of a number of key cellular regulatory pathways, including cell proliferation pathway, cell survival pathway, caspase activation pathway, tumor suppressor pathway, death receptor ... pathway, mitochondrial pathway and protein kinase pathway Most cells apoptosis pathway is through mitochondrial-mediated pathway, which is mostly regulated by Bcl-2 family, including the antiapoptotic...

Ngày tải lên: 20/06/2014, 03:20

... secretory IgA concentration was done by the sandwich ELISA [8,32] S tatis tica l an a ly sis Data were analysed by one-way analysis of variance followed by Duncan's multiple-range test to identify ... CLA2 and CVA2 had a significant decrease compared to control CS2 was slightly increased compared to other 1.5% CLA groups, but there was no significant difference among them CVA1, CVA2, CS1 and ... hours, the samples were allocated into 50㎕ at -80℃ until IgA analysis P re p ara tion o f th e gu t lu m e n la va ge After blood collection, the end of the duodenum and the cranial part of the...

Ngày tải lên: 07/08/2014, 17:22

... pairs [bp], 59°C), RARα (sense: 5'-ACCAGATTACCCTTCTCAAGG-3', anti-sense: 5'-GGTACCGCGTGCAGATT-3', NM 031528, 65 bp, 58°C), RAR-β (5'-TCTTAATGAAAATCACAGATCTCCGC-3', antisense: 5'-CCAGGAATTTCCATTTTCAAGG-3', ... Nakajima T, Kinoshita S, Sasagawa T, Sasaki K, Naruto M, Kishimoto T, Akira S: Phosphorylation at threonine-235 by a rasdependent mitogen-activated protein kinase cascade is essential for transcription ... 5'-CTGCACCGGGCAGGGTGGAAT-3', anti-sense: 5'-CTGGACGGAAACCGAGCGGTG-3', NM 031765, 65 bp, 66°C), and RP29 (sense: AAGATGGGTCACCAGCAGCTCTACTG-3', antisense: 5'-AGACGCGGCAAGAGCGAGAA-3', NM 012876, 70 bp, 59°C) The quantity...

Ngày tải lên: 09/08/2014, 01:22

... symbols) The data are mean values (and standard deviations) from three independent determinations for each treatment modality The survival data after combined treatment are normalized to SAHA toxicity ... that cell cycle regulation changes and a p53-independent apoptotic pathway are the major mechanisms for the sensitization to HIT by SAHA We further assessed apoptosis 24 and 48h after HIT 12 Apoptosis ... the data and draft the manuscript, AEK aided in study design and provided the necessary laboratory equipment, VE performed experimental procedures (FACS analysis) and helped to analyze the data,...

Ngày tải lên: 09/08/2014, 09:21

... TW and YS have carried out the study design, statistical analyses and drafted the manuscript All authors read and approved the final manuscript Competing interests The authors declare that they ... 24 Taguchi T, Sonobe H, Toyonaga S, Yamasaki I, Shuin T, Takano A, Araki K, Akimaru K, Yuri K: Conventional and molecular cytogenetic characterization of a new human cell line, GIST-T1, established ... completely the migration of these cells (data not shown) All together, the data suggested that ATRA may be useful to prevent the invasion or metastasis of GIST cells Cytotoxic effect of combination...

Ngày tải lên: 10/08/2014, 10:20

... gratitude to Dr Sudip, Dr Rakesh, Dr Meng Tack, Dr Tian Lu, Dr Wei Lee, Dr Mangayarkarasi, Abdul, Saravanan, Goutam, Jeremiah, Raghavendar and Anjana for their invaluable support, suggestions and ... organic ligands extending in an array The design and synthesis of metal-organic coordination polymers has attracted special interest due to their potential applications in gas storage/separation, ... 19 a) A view showing the connectivity of the nodal unit giving a two-dimensional (4,4) square grid b) A graphical diagram representing a (4,4) square grid in the ab-plane a) A graphical diagram...

Ngày tải lên: 11/09/2015, 10:06

... saturated fatty acids with chain length of C12:0-C16:0 are atherogenic, stearic acid is neutral, and oleic and polyunsaturated fatty acids have a lipid lowering effect Total saturated fatty acids ... found regarding total SFA (saturated fatty acids), and PUFA (polyunsaturated fatty acids) SFA were around 34% to 45% of total fatty acid methyl esters; cisPUFA, around 13% to 20% of total methyl esters ... samples of triplicates * Fatty acids less than 0.1%: C15:0, C17:0, C21:1 * SFA: saturated fatty acids * PUFA: polyunsaturated fatty acids * TFA: trans fatty acid* MUFA: monounsaturated fatty acids...

Ngày tải lên: 28/08/2013, 16:28

... and transistor power converters II ELECTROMAGNETIC VIBRATORY ACTUATOR (EVA) All main types of vibratory actuators can be seen as two-mass systems The majority of them generate harmonic excitation ... for cases of phase angle control and switch-mode current control are presented Parameters of the real actuator and vibratory system are chosen Electrical and mechanical parameters of the EVA are ... of the above-mentioned vibratory actuators are shown in Fig The mathematical model of EVA is based on presentation in Figs 2 (a) and with details An electromagnet is connected to an ac source and...

Ngày tải lên: 03/01/2014, 19:16

... used to further characterize the specific binding modes of these 1,3-BPGA analogues to the two trypanosomatid enzymes Materials and methods Sources of substrates, cofactors and inhibitors The synthesis ... codon of the T brucei gGAPDH gene; an antisense primer 5¢-CAGCCAAGCGCCTAGGGAGCGAGA AC-3¢, containing a BamHI site (underlined) and starting 31 nucleotides downstream of the stop codon The total volume ... catalysis The average isotropic temperature factor values for the main chain and all atoms of the 359 residues from ˚ ˚ each monomer are, respectively, 43.5 A2 and 43.8 A2 in ˚ ˚ ˚ monomer A, ...

Ngày tải lên: 20/02/2014, 02:21

... format MAT, malonyl-CoA transacylase domain; KS, b-ketoacyl synthase domain; KR, b-ketoacyl reductase domain; DH, dehydratase domain; ER, b-enoyl reductase domain; ACP, acyl-carrier protein domain; ... Inactivation of hypothalamic FAS by C75 is linked to satiety and dramatic weight loss [15] because accumulation of the substrate malonyl CoA through the inhibition of FAS appears to inhibit the ... Cruz, CA, USA) Unless otherwise stated, all chemical reagents were from Sigma-Aldrich FAS activity assay FAS activity was assayed by a spectrophotometric method using a Synergy Microplate Reader...

Ngày tải lên: 06/03/2014, 09:22

... (RVV)-7, a cytotoxin with phospholipase A2 (PLA2) activity [17] that also acts as a renal tubular necrosis factor [18] Empirical assay protocols for hemorrhage and PLA2 activity that are suitable ... combinations of OAB OAB, OAB NAT and OAB NATA were applied to L1 and L2, considering OAB as inhibitor (I1) and OAB, NAT and NATA as inhibitor (I2) In these experiments, the substrate concentration ... group and amidation of the carboxylate group in Trp result in a substrate analog N-acetyl-l-tryptophan amide (NATA) with neutralized charged groups, whereas N-acetyltryptophan (NAT) bears a carboxylate...

Ngày tải lên: 07/03/2014, 05:20