9 conclusions and future trends in the dairy industry

Separation, extraction and concentration processes in the food, beverage and nutraceutical industrie

Ngày tải lên : 20/09/2015, 01:57

... dairy industry 12.8 Treatment of effluents and technical fluids in the dairy industry 12.9 Conclusions and future trends in the dairy industry 12.10 The egg products industry ... proteins in the dairy nutraceutical industry 16.6 Immunoglobulins in the dairy nutraceutical industry 16.7 Purification technologies for immunoglobulins in the dairy nutraceutical industry ... casein in the dairy industry 12.6 Separation techniques applied to whey and derivatives in the production of cheese 12.7 Fractionation of individual proteins and peptides in the dairy...

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Báo cáo khoa học: Apoptosis-inhibiting activities of BIR family proteins in Xenopus egg extracts ppt

Ngày tải lên : 30/03/2014, 16:20

... apoptosis-inhibiting function, and the further addition of the residue 270–401-containing PEST2 and RING finger domain resulted in rapid proteolysis Therefore, the apoptosis-inhibiting potential ... (compare Figs 5A and 6C) Thus, in both xEIAP ⁄ XLX and xXIAP, the C-terminal region containing the RING finger domain is required for the initial phase of proteolysis within h and also for the final degradation ... extracts containing cycloheximide and incubated for 0, 2, and h in the presence of dimethylsulfoxide (DMSO), 100 lM roscovitine, and 100 lM U0126 p150Glued and the remaining MBP fusion proteins were...

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Extraction of anthocyanins from industrial purple ﬂeshed sweetpotatoes and

Ngày tải lên : 05/05/2014, 08:39

... other common anthocyanin containing fruits and vegetables, the concentration of anthocyanins in purple-fleshed ISPs are in the same range as some of the highest anthocyanin producing crops like blueberries, ... to represent the change in starch content as a percent of dry matter and was examined in this study to determine the amount of starch converted during hydrolysis In this case, the main effects ... fraction of the ISP dry matter, assuming the enzymes are not degrading protein and fiber (difference between initial and final AIS values) Protein and fibrous fractions of the ISP dry matter were not measured...

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Báo cáo hóa học: " Localization studies of two white spot syndrome virus structural proteins VP51 and VP76" docx

Ngày tải lên : 20/06/2014, 01:20

... understanding of the molecular mechanism of WSSV infection and assembly, or be helpful for the control of virus infection in the future Conclusion The localization of the two proteins, VP51 and ... proteins, VP51 and VP76, in WSSV virions by employing multiple approaches, and make a definite conclusion, i.e VP51 and VP76 reside in the viral nucleocapsid, and are viral minor capsid proteins The ... of VP51 and VP76 by Western blotting The truncated recombinant proteins, VP51p and VP76p, were expressed in Escherichia coli and purified by using NiNTA affinity chromatography, and then specific...

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Báo cáo y học: "Eggshell and egg yolk proteins in fish: hepatic proteins for the next generation: oogenetic, population, and evolutionary implications of endocrine disruption" pptx

Ngày tải lên : 13/08/2014, 13:20

... protein synthesis Eggshell zona radiata proteins and the egg yolk protein precursor, vitellogenin are synthesized and secreted by the hepatocyte They are transported in blood to the ovary and incorporated ... is the synthesis of the oogenic proteins, Vtg and Zrproteins E2 is the major estrogen in female fish E2 stimulates the production of Vtg and Zr-proteins in the liver The genes encoding these fish ... re-organized through multiple insertions and deletions of intervening sequences during the evolution of the various lineages Characterization of the Vtg region in the genome of the rainbow trout, e.g.,...

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Báo cáo y học: "Identification of Cellular Membrane Proteins Interacting with Hepatitis B Surface Antigen using Yeast Split-Ubiquitin System"

Ngày tải lên : 02/11/2012, 11:08

... domain The fusion plasmids are then cloned into the yeast cell and expressed on the cell membrane If the prey protein interacts with the bait protein, this would bind the NubG and Cub domains ... limitation [6-8] In this system, the ubiquitin is split into N-terminal ubiquitin (Nub) and C-terminal ubiquitin (Cub) but the Nub and Cub have high affinity towards each other and is able to reassemble ... reassemble back together spontaneously into ubiquitin protein When Nub and Cub moieties bind together within a cell, this would activate the ubiquitin-specific protease (UBP) and hence, the transcriptional...

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Comparison of Methods for the Extraction of Bioflocculants from Activated Sludge

Ngày tải lên : 05/09/2013, 09:08

... Ag-protein stain, the components varied greatly by polymer Several bands demonstrated the same mobility in both stains, suggesting that these bands were complexes of protein and saccharide These included ... included the band corresponding to a molecular weight of 103,000 in the steaming-extracted polymer (1), the band corresponding to 38,000 in the NaOH-extracted polymer (2), the band corresponding to ... determined using the molybdenum-H2SO4 method (Schnittger et al., 1959) Protein was determined according to the method of Lowry et al (1951) using a bovine serum albumin standard Nucleic acid and...

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Tài liệu Activity 12.1: Risks of No Functional Specification doc

Ngày tải lên : 24/01/2014, 10:20

... 1: Creating an Origami Boat ! Create an origami boat Construct an origami boat using the sheet distributed by the instructor Fold the paper along the lines to create the boat The order and directions ... boat The order and directions for folding are documented in the functional specification After completing the above steps, you will discuss the activity with the class Activity 12.1: Risks of No ... the activity with the class Activity 12.1: Risks of No Functional Specification 105 THIS PAGE INTENTIONALLY LEFT BLANK ...

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Tài liệu Báo cáo khoa học: A knowledge-based potential function predicts the speciﬁcity and relative binding energy of RNA-binding proteins ppt

Ngày tải lên : 18/02/2014, 16:20

... extracted the protein coordinates and the four nucleotides bound at the center of the domain (for structures containing more than one RNA-binding domain, the structure was divided into two independent ... 0.05 a The native U1A complex was included in the training set for this experiment b The U2B¢ complex (U1A homolog) was included in the training set for this experiment and have scored these structures ... protein mutants and RNA containing cytosine at position )5 (C) The characteristic intramolecular hydrogen bond between the amino group of C5 and the O1P atom of U6 observed in the structure of the...

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Tài liệu Báo cáo khoa học: Analysis of the molecular dynamics of medaka nuage proteins by ﬂuorescence correlation spectroscopy and ﬂuorescence recovery after photobleaching doc

Ngày tải lên : 18/02/2014, 16:20

... of the constructs microinjected into the medaka eggs and transfected into HeLa cells The olvas, nanos and tudor coding sequences were joined to the C-terminal region of the GFP or RFP coding ... expressed in the medaka PGC at stage 24 and localized in the nuage 3D imaging of Olvas GFP was performed at the indicated times (B) The nuage was seen around the nucleus and came together and apart in ... at time point t, T0 and Tt represent the uorescence in the whole cell at time points and t, respectively, and B0 and Bt represent the uorescence in the bleached region at time points and t Diffusion...

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Tài liệu Báo cáo khoa học: Structural bases for recognition of Anp32⁄LANP proteins doc

Ngày tải lên : 18/02/2014, 17:20

... spectrum, is routinely used to detect interactions and map them on the structures of the individual components Fig Probing the interaction between the Anp32a LRR domain and the AXH domain of Atx1 by ... each other are mainly perpendicular to the b-sheet vectors and, therefore, to B0 in the aligned medium These results suggest that interactions with other molecules involving the LRR domain are ... and ﬂexible method for mapping binding interfaces Our structure, as described in the following sections, reveals the presence of two extra N-terminal LRR motifs not observed in the crystal, and...

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Tài liệu Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas docx

Ngày tải lên : 19/02/2014, 00:20

... mainly expressed during larval metamorphosis, in the mantle edge and the digestive gland During the reproductive cycle, expression was high in gonads during the postspawning period but not in ... from the oyster Crassostrea gigas [5] Interestingly, this protein, named C gigas chitinase-like protein (CgClp1) was found to be involved in the control of growth and remodelling processes in a ... blocked by incubating sections in 0.3% hydrogen peroxide in methanol for 30 at room temperature Slides were then washed and incubated in a blocking solution according to the manufacturer’s instructions...

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Tài liệu Báo cáo khoa học: Binding of N- and C-terminal anti-prion protein antibodies generates distinct phenotypes of cellular prion proteins (PrPC) obtained from human, sheep, cattle and mouse doc

Ngày tải lên : 19/02/2014, 02:20

... out in the linear range determined using serial dilutions of samples (Fig 2) Linearity consisting of continuous signal increase and of reproducible glycoprotein patterns was determined in the ... antibodies binding to the core region (i.e amino acids 121–166; C-terminal region) The di-glycosylated band of human PrPC showed the heaviest staining with antibodies binding to the N-terminal region ... C-terminal binding antibodies recognized two bands comprising full-length PrPC and an 18–19 kDa protein band corresponding to the N-terminally truncated form The distribution of the signal intensities...

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Tài liệu Báo cáo khoa học: Analysis of oxidative events induced by expanded polyglutamine huntingtin exon 1 that are differentially restored by expression of heat shock proteins or treatment with an antioxidant ppt

Ngày tải lên : 19/02/2014, 06:20

... of the initiator ATG in exon (Ex1) of the 67 exon-containing htt gene [5] Pathogenesis in HD correlates with the cleavage of mutated htt and the release of an N-terminal fragment bearing the ... arrow indicates the position of the more intensively oxidized polypeptide in the assay The bracket underlines the domain (molecular mass range 10–40 kDa) of the oxyblots that contains the greatest ... abolished the increase in NO• levels In contrast, Hsps were not efﬁcient in httEx1-103Q-EGFP-expressing cells, but NAC did abolish the NO• increase induced by both httEx1- Huntingtin inclusions and...

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Tài liệu Báo cáo khoa học: Selection of stably folded proteins by phage-display with proteolysis docx

Ngày tải lên : 19/02/2014, 12:20

... N-terminal domains (N1, N2) of the minor coat protein (g3p) are responsible for binding and infection in E coli Thus, incorporation of a library of target proteins between the N-terminal domain and ... that are originally packed between the fourth and the third helices in the wild type protein, to participate in the new hydrophobic core of the structure The two hydrophilic residues in the selected ... of target proteins with a tag can be fused to the g3p protein on the surface of the phage The tag can be a His-tag [11] or antibodybinding proteins such as the protein AB-domain [18] Removal...

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Tài liệu Báo cáo khoa học: Subproteomics analysis of Ca2+-binding proteins demonstrates decreased calsequestrin expression in dystrophic mouse skeletal muscle pdf

Ngày tải lên : 19/02/2014, 16:20

... dye staining solution The dye-containing solution was heated to 90 °C and carefully poured over the 2D gel in a stainless steel tray The tray was then placed on top of a hot plate and the temperature ... temperature maintained at 90 °C for to aid the staining of protein spots The tray was then placed on a laboratory shaker for a further 10 at room temperature Destaining was achieved by placing gels in a ... and were stored at °C until further usage For silver staining, the PhastGel protein silver staining kit was used (omitting glutaraldehyde from the sensitizing solution and formaldehyde from the...

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Tài liệu Báo cáo khoa học: "Cross-Domain Co-Extraction of Sentiment and Topic Lexicons" pdf

Ngày tải lên : 19/02/2014, 19:20

... Portland, Oregon ACL Minqing Hu and Bing Liu 2004 Mining and summarizing customer reviews In Proceedings of the tenth ACM SIGKDD international conference on Knowledge discovery and data mining, ... the proposed two-stage domain adaptation framework: 1) generating some sentiment and topic seeds in the target domain; and 2) expanding the seeds in the target domain to construct sentiment and ... domain and the sentence the movie is excellent” in the movie domain, respectively As can be observed, the relationships between the topic and sentiment words in the two sentences are the same They...

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Tài liệu Báo cáo khoa học: "Automatic Extraction of Lexico-Syntactic Patterns for Detection of Negation and Speculation Scopes" pdf

Ngày tải lên : 20/02/2014, 04:20

... split into subsets (preceding vs following their scope) to identify cues using string matching The cue scopes extend from the cue to the beginning or end of the sentence, depending on the cue ... present in the dataset The scopes of cues that are missing from the baseline system were not included in the results As the baseline system was not penalized for missing cue phrases, the results ... Statistics of the BioScope corpus The 2nd and 3d columns show the total number of cues within the datasets; the 4th and 5th columns show the percentage of negated and speculative sentences 70% of the corpus...

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Tài liệu Báo cáo khoa học: "Robust Extraction of Named Entity Including Unfamiliar Word" doc

Ngày tải lên : 20/02/2014, 09:20

... described in (Asahara and Matsumoto, 2003) and features of surrounding phrases described in (Nakano and Hirai, 2004) We will in1 28 vestigate incorporating SF and these features in the near future ... of extracting NEs which consist of unfamiliar morphemes The column “Other” shows the results of extracting NEs which contain both familiar morpheme and unfamiliar one These results indicate that ... derived labels from the label B and the label I for eight NE types, in order to distinguish them When the task of extracting Japanese NEs from a sentence is formalized as a chunking problem of a...

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Tài liệu Báo cáo Y học: A Raman optical activity study of rheomorphism in caseins, synucleins and tau New insight into the structure and behaviour of natively unfolded proteins pot

Ngày tải lên : 21/02/2014, 15:20

... helical conformation There may also be some b strand in some of the proteins, especially b- and j-casein judging by the wellde®ned negative ROA bands in these proteins in the range » 1245 cm)1, ... residues, as observed in the caseins [3], synucleins [10], tau [14], prothymosin a [7] and the ®bronectin-binding protein [8], and also in typical chemically denatured proteins [56±58] Over extensive ... Some of the negative ROA intensity in the range » 1240±1266 cm)1 may be due to b strand These data suggest that, as in the caseins and synucleins, the major conformational element present in the...

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