826 general structure of a counter s state diagram—a single cycle

... Structure analysis and comparison Software for structure analysis and comparison was chosen from the CCP4 suite and O Numbering in the comparisons refers to SPRK unless stated otherwise The alignment ... classical cold adapted features [19], but still initial comparative studies showed that the catalytic turnover was at least twice that of PRK, but substrate affinity was reduced SPRK was compared ... substrate analogues are observed in the structures of serine peptidase complexes Instead it is located in the S1 ¢ and S2 ¢ sites, and Thr3 and Pro2 occupy the same space as P1¢ and P2¢ residues found...

Ngày tải lên: 19/02/2014, 07:20

... mistakes that learners easily make in specific situations Last but not least, a number of analytical instruments helps both teachers and learners deal with grammatical, semantic, and discoursal ... Clause as Messages In each metafunction, an analysis of a clause gives a different kind of structure composed from a different set of elements In the ideational metafunction, a clause is analyzed ... to as Behaver, and this participant is always a conscious being For example: She cried Behaver softly Process Behavioural Circumstance Relational processes are the processes of being, being at,...

Ngày tải lên: 07/09/2013, 13:48

... meaning and structure of a science fiction story: A sysyemic 29 Clauses and Clause Complexes Analysis The analysis of the text into clauses and clause complexes and their logico-semantic relations ... lexical cohesion analysis is based on Halliday and Hasans (1976) and Hasans (1984) procedures The analysis focuses on such lexical cohesive ties as repetition, antonym, meronym, synonym, syper-ordinate ... the analysis of a science fiction story, using systemic functional linguistics as the theoretical framework As can be seen from the analysis, systemic functional linguistics is a model of language...

Ngày tải lên: 12/02/2014, 20:20

... scaffold characteristic of subtilisin-like serine proteinases It consists of six a helices, one ⁄ 10 helix, a b sheet made of seven parallel strands and two b sheets made of two antiparallel strands ... was done in xtalview [76] Water molecules were assigned with arp ⁄ warp [77] using standard parameters Refinement statistics are shown in Table Structure analysis Superposition of structures was ... temperatures To address questions regarding the proposed role of the increased exposed apolar surface as a mechanism of cold adaptation, it should be considered that interactions of such surfaces...

Ngày tải lên: 19/02/2014, 16:20

... eigenvectors, nine classes were discriminated on the basis of similarity of features after rotational alignment without symmetrization Most class averages showed a star-shaped structure with six-fold symmetry ... images were classified on the basis of 5364 eigenvector–eigenvalue data analysis, and subsequent averaging was performed for each class separately The average was finally symmetrized on the basis ... apomonomers and FAD at room temperature for [26], was also measured These assays revealed that NADH oxidase activity was slightly higher than that of NADPH oxidase, and FAD significantly restored...

Ngày tải lên: 07/03/2014, 04:20

... kinases, a MAP kinase (MAPK), a MAPK kinase (MAPKK) and a MAPKK kinase (MAPKKK), are common signalling modules in eukaryotic cells [20,21] The budding yeast (S cerevisiae) has several MAPK cascades ... Waskiewicz AJ & Cooper JA (1995) Mitogen and stress response pathways: MAP kinase cascades and phosphatase regulation in mammals and yeast Curr Opin Cell Biol 7, 798–805 22 Brewster JL, de Valoir ... osmolarity glycerol response) pathway for hyperosmolarity adaptation in budding yeast (Saccharomyces cerevisiae) and based on the related mRNA expression time-series data from Stanford Microarray...

Ngày tải lên: 07/03/2014, 21:20

... al crystal structure of a cellulase was reported; it was a catalytic domain of Hypocrea jecorina Cel 6A (HjeCel 6A, formerly designated cellobiohydrolase II), a GH6 cellobiohydrolase from an ascomycete ... overall structure of CcCel6C alone is shown in Fig 1A Like the fungal GH6 cellobiohydrolases [6,7], CcCel6C consists of a sevenstranded b ⁄ a barrel fold a- Helices and b-strands are numbered as a1 a8 ... potential catalytic residues and could act as a proton donor and a base, respectively Another aspartic acid residue (Asp175 of HjeCel 6A) has been proposed to contribute to the electrostatic stabilization...

Ngày tải lên: 15/03/2014, 10:20

Ngày tải lên: 17/03/2014, 14:28

... organicrinorganic hybrid phases are obtained and structurally identified using the single crystal X-ray diffraction method Brief crystal data of one of the phases, a mono- Results and Discussion The ... quality and morphology The reaction is then rationalized by using Na 2WO4 as W 6q source instead of WO that gives rise to monophasic product of compound containing large single crystals TGA shows a ... reflections 0.90 A w I ) s Ž I x for 134 parameters and converged to R 1Ž wR s 0.0281Ž0.0634 Atomic coordinates, bond lengths and angles and thermal parameters are presented in supplementary crystallographic...

Ngày tải lên: 19/03/2014, 16:48

... the availability of health care services in underserved areas The IHS Health Professions Scholarship Program and IHS Loan Forgiveness Program improve access to medical care for Indian and Alaska ... transportation services are also granted a one-year extension of the placed-in-service deadline Special rules apply to syndications, sale-leasebacks, and transfers to related parties of qualified ... suffer an economic disruption as a result of a major job loss event, such as a military base closing or manufacturing plant closing Reasons for Change The loss of a major employer can devastate a...

Ngày tải lên: 23/03/2014, 02:20

... sleeping sickness (Trypanosoma brucei), Chagas disease (Trypanosoma cruzi), and leishmaniasis (Leishmania species)] Note that some euglenozoans, such as E gracilis, also contain a chloroplast with ... ubiquitous, free-living phagotrophic flagellates (e.g Bodo saltans), photosynthetic algae (e.g Euglena gracilis), and parasitic trypanosomatids [e.g the causal agents of the tropical diseases African sleeping ... fasciculata, a monogenetic insect parasite that is not pathogenic to humans Some trypanosomatids (e.g Leishmania spp and T cruzi) possess a classic mitochondrial respiratory chain [14–16] In others...

Ngày tải lên: 23/03/2014, 04:21

... sugars The Michaelis constant (Km) and specific activity were calculated from a plot of initial reaction rates versus substrate concentration using Prism (GraphPad Software, San Diego, CA, USA) ... collection, a crystal was transferred into Analysis of substrate specificity Substrate specificity was analyzed using xyloglucan tetradecasaccharide, XXXGXXXG, prepared as described previously [8] ... Yaoi et al (1 fi 6)-b-d-Glcp Other sugars such as galactose, arabinose and fucose may also be present on the side chains in various branching patterns, depending on the plant species Structural...

Ngày tải lên: 23/03/2014, 05:22

... the active site structure and composition are identical to those of mesophilic b-lactamases This indicates that the active site of the Pse fluorescens b-lactamase is more easily accessible to large ... separate samples of the protein with Lys-C proteinase, N-Asp protease, and Arg-C protease The correctness of the sequence of each peptide was controlled by mass analysis using a Tofspec SE MALDI-TOF ... Life Sci 53, 830–841 Matsumura N, Minami S & Mitsuhashi S (1998) Sequences of homologous beta-lactamases from clinical isolates of Serratia marcescens with different substrate specificities Antimicrob...

Ngày tải lên: 23/03/2014, 07:20

... 5’-CATCCTTCCCTATCTCAACATCCAGCTGGTTTACT-3’ D341N-1 5’-AAGGGGAGAACTCAACGGAACACCGGAGG-3’ 5’-CACTCTCGAAGAGTTCATAAACGAAGTGAAGAAGAATCTC-3’ 5’-GAGATTCTTCTTCACTTCGTTTATGAACTCTTCGAGAGTG-3’ F205M D36N + D341N D389N FEBS ... 5’-CCTGGAGAAATCGATCATGAGCTTCGCTCAGTCGTG-3’ 5’-CACGACTGAGCGAAGCTCATGATCGATTTCTCCAGG-3’ 5’-AAAAGGTCGACATCTGGATGGCGACGAAAGACACGATC-3’ 5’-GATCGTGTCTTTCGTCGCCATCCAGATGTCGACCTTTT-3’ D36N-1 5’-CATCCTTCCCTATCTCAACATCCAGCTGGTTTACT-3’ ... structural assignments made using the program dssp [72] Surface and volume calculations and analysis of noncovalent interactions Ion pairs and ionic networks were analysed using the programs contact...

Ngày tải lên: 23/03/2014, 10:21

... systems [13] In some cases, as in the treatment of oral leukoplakia lesions, the use of BBIs has reached phase II of clinical trials [14,15] Besides the anticarcinogenic effects, BBIs also showed anti-inammatory ... molecular basis of these BBI activities has not been established so far, however, because a high protease activity has been shown to be connected with tumour formation and other diseases associated ... times A correlation time optimum value of ns was found by systematic search, assuming isotropic motions A further selection of the best 24 structures was made on the basis of total energy Finally,...

Ngày tải lên: 23/03/2014, 10:21

... proposed catalytic residues type of mechanism, as shown by Davies & Henrissat [9], ˚ involves a proton donor and a base separated by  5.5 A [59–61] Henrissat [62] classified all members of GH7, ... that the structures of T reesei GH7 CBHs were solved in the presence of substrates However, as 1Q9H was solved in the absence of bound substrate, one could imagine that if a substrate was present ... Desmet, T & Claeyssens, M (2003) A hydrophobic platform as a mechanistically relevant transition state stabilising factor appears to be present in the active centre of all glycoside hydrolases...

Ngày tải lên: 23/03/2014, 13:20

... surface areas are calculated with a probe radius 1.4 A added to the van der Waals radius Dimer model Buried surface Ê area (A2 ) Hydrogen bonds Salt bridges A 77Arg A 65Glu A 65Glu A 61Glu A 58Glu ... (1993) Interaction between staphylokinase, plasmin(ogen), and alpha2-antiplasmin Recycling of staphylokinase after neutralization of the plasmin-staphylokinase complex by alpha2-antiplasmin J Biol ... possible dimer geometries, designated as a a, head±tail, and b±b The a a dimer has a diad and is characterized as helix-helix packing between the two monomers, as shown in Fig 2A The head±tail...

Ngày tải lên: 23/03/2014, 21:21

... This result illustrates the natural tendency of TPR domains to stack head to tail and self-assemble into an ordered macrostructure in crystals We have seen no evidence for such association in solution ... cocrystal structure of a designed TPR domain with its partner peptide We show that this 3-TPR domain can adopt a superhelical structure in the crystal similar to those reported for long TPR arrays ... gas ˚ stream (100 K) Data were collected to 2.85 A resolution at the NSLS beamline X12C (Brookhaven National Laboratory) The data collection statistics are shown in Table Structure determination...

Ngày tải lên: 29/03/2014, 08:20

... conformational changes of protein structures at submolar denaturation concentrations The structural changes of horseradish peroxidase and spectrin at submolar concentrations of denaturant are examples reported ... Enzyme activity assay FB188 HDAH exhibits amidohydrolase [28] and esterase activity [48] Amidohydrolase activity was assayed in the two-step assay [49,50] As trypsin activity is required in this type ... this type of assay, and trypsin rapidly denatures upon addition of denaturant, the two-step assay was not suited for activity measurements in samples containing Gdn-HCl Esterase activity was monitored...

Ngày tải lên: 30/03/2014, 08:20

... restraints regarding disulfide bridges Analysis of Ca–Ca and Sc–Sc distances between cysteines observed for the resultant structures led to identification of disulfide bond pairings of the 4-kDa ... cross-reacted bands were detected with alkaline phosphatase substrate (Moss, Maryland) Results and discussion Possible physiological function of the 4-kDa peptide To investigate the physiological ... systems, which are similar to the animal systems Yamauchi, F., Sato, K & Yamagishi, T (1984) Isolation and partial characterization of a salt-extractable globulin from soybean seeds Agric Biol Chem...

Ngày tải lên: 31/03/2014, 07:20