816 vhdl program for counter cell of figure845

... μM of each primer, 200 μM of each dNTP, 2.5 μM MgCl2, 0.025 U/μl of Taq DNA polymerase (Fermentas, MMedical, Milan) PCR conditions were 95°C for followed by 30 cycles at 95°C for 45 sec, 55°C for ... (5'-GCA-TGA-CTC-AAA-AAA-CTT-AGCAAT-TCT-G-3') The target cells were confluent monolayers of SV40immortalised rabbit autologous skin cells (106 cells/5-cm Petri dish) infected with 10 PFU /cell of the FPE6 or FPE7 recombinants ... well at the effector-to-target -cell (E:T) ratios of 30:1 and 15:1 The plates were centrifuged for at 250 × g, and the cells were incubated at 37°C for h A volume of 50 μl supernatant was transferred...

Ngày tải lên: 18/06/2014, 16:20

... of mutagens that were not detected in the standard tester strains They are suitable for mutagenicity of aromatic hydrocarbons, allyl hydrocarbons and insecticides, but not suitable for that of ... Identification of C8-modified deoxyinosine and N2- and C8-modified deoxyguanosine as major products of the in vivo reaction of N-hydroxy-6-aminochrysene with DNA and the formation of the adducts ... We compared the mutagenic activity (shown as the number of revertants per nanomolecule of chemicals, rev./nmol) of YG strains with that of the conventional TA strains as shown in Fig YG1041 showed...

Ngày tải lên: 05/09/2013, 08:40

... functional status of cells is now routinely performed for plant cell cultures in order to permit accurate assessment of growth and metabolite production rates The availability of plant cells for quantitative ... development of an information base on genetic, cellular, and molecular levels is now a prerequisite for the use of plants or plant cell cultures for biotechnological applications for the following ... patterns, bioinformatics is used here for this type of analysis of complex systems Bioinformatics refers to the extraction of biological information from genomic sequence and the reconciliation of multiple...

Ngày tải lên: 25/10/2013, 05:20

... expression in human cell lines We initially analyzed the effects of hypoxia on the expression of HO-1 and HO-2 in human cell lines of Reduced expression of heme oxygenase-2 A B Fig Effects of hypoxia ... oxygen) for 48 h The cell extracts were prepared for western blot analysis of HO-1 and HO-2 The lane labeled h contained cell extracts prepared from untreated cells harvested just before starting ... expression levels of HO-2 mRNA and protein in five out of six cell lines, with the exception of KG1 myeloid cells A Hypoxia decreases the expression levels of HO-2 protein in YN-1 and K562 cells To confirm...

Ngày tải lên: 19/02/2014, 06:20

... III Cell treatment with the heparinase III GAG lyase (Sigma) was performed as previously described [21] However for easier handling of the cells, treatment was performed on HeLa cells instead of ... not cell speci®c as a large number of cell lines tested so far entrapped the translocating peptide Fig In¯uence of temperature on the uptake of Tat CPP and of Tat±GFP fusion protein HeLa cells ... Travo for his help in ¯uorescence imaging and computerized analysis of pictures We are grateful to Dr Jean-Jacques Vasseur for performing MALDI-TOF analysis of peptides We also thank I Robbins for...

Ngày tải lên: 21/02/2014, 03:20

... use of this drug for sickle cell disease Therefore, we sought information on barriers to the use of other therapies for treatment of sickle cell disease, including the receipt of routine, scheduled ... methodological quality of the MSH was excellent (Jadad score of 5, out of a maximum of 5) and that of the Belgium study was moderate (Jadad score of 3), because the method of masking was not described ... person-year for Hb SC disease In a study of 1,056 patients with Hb SS disease in California, 70 percent of patients were admitted for a crisis; the overall rate of hospitalizations for crisis...

Ngày tải lên: 05/03/2014, 10:20

... the percentage of cells within a population that are actively undergoing apoptotic cell death For the cell- death assessment, the cells were plated in 60 mm dish at a density of · 105cells per dish ... control (G) Effect of overexpression of an Ser70-specific mutant form of Bcl-2 (S70A) on radiation-induced Bcl-2 phosphorylation HeLa cells transfected with the Ser70-specific mutant form of Bcl-2 (S70A) ... control (H) Effect of overexpression of the Ser70-specific mutant form of Bcl-2 (S70A) on radiation-induced loss of mitochondrial transmembrane potential and apoptotic cell death HeLa cells transfected...

Ngày tải lên: 07/03/2014, 05:20

... research was conducted under the auspices of the Graduate School of Wageningen Institute of Animal Sciences (WIAS) Optimisation of selective breeding program for Nile tilapia (Oreochromis niloticus) ... submitted in fulfilment of the requirements for the degree of doctor at Wageningen University by the authority of the Rector Magnificus Prof.dr M.J Kropff, in the presence of the Thesis Committee ... correlations of this trait with growth rate and shape would be of added value for the breeding program 14 General introduction The GIFT breeding program is conducted by the Research Institute for Aquaculture...

Ngày tải lên: 13/03/2014, 18:58

... decision-making for future plans for adolescents Purpose of the study This study’s purpose was to evaluate a reproductive health awareness program for the improvement of reproductive health for unmarried ... was shown in either of the tests for the boys Comparison before and after the program Table describes the scores of knowledge, attitude, and behavior before and after the program The scores in ... Page of that they wanted more time for discussion, (2) because the program evaluation was conducted immediately after the program, we cannot exclude the possibility of retention Efficacy of reproductive...

Ngày tải lên: 22/03/2014, 12:20

... [fluorescence activated cell sorting (FACS)], a significant increase of CD98 expression on intact BeWo cells after forskolin treatment for 24 h [18] Here, we determined the levels of expression of CD98 by ... control cells) was not substantially different from that in cells treated with 100 lm forskolin after 12 h of culture After 24 h, whereas there was no increase in control cells, the addition of forskolin ... (arrow) of  64 kDa (A1) or  80 kDa (B) was present in whole cell lysates from BFA-treated cells in both control and forskolin-stimulated cells, presumably a partially glycosylated form of CD98...

Ngày tải lên: 23/03/2014, 09:20

... T of S is a subsemigroup of S if the restriction of S × S → S maps T × T into T The intersection of any family of subsemigroups of S is again a subsemigroup of S If G is any set of elements of ... collection of units U and a homomorphism T defined on sequences of elements of U In other words, U is the set of generators of the free semigroup that is the domain of T Suppose that a and b are two of ... product of any sequence s1, s2, ,.sn of elements of a semigroup S is an element of S defined inductively in terms of the binary composition, and is shown to be independent of the association of...

Ngày tải lên: 23/03/2014, 13:20

... efficiency of protein N-myristoylation and the identity of the amino acids at positions 3, and of the consensus motif in plant target proteins with the use of a Cell- free N-myristoylation of plant ... the cell- free system, we next performed MALDI-TOF MS analysis Wild-type forms of ARF1A1c and Myr–GFP were synthesized in vitro and purified by Ni2+-affinity column chromatography on the basis of ... et al Cell- free N-myristoylation of plant proteins agreement with the calculated mass for the corresponding myristoylated N-terminal fragments (m ⁄ z ratios of 818.1 for ARF1A1c and 1184.5 for...

Ngày tải lên: 29/03/2014, 21:20

... galectins [24]; (b) tested as ligand for surface receptors of different types of tumor cells; and (c) injected into circulation with monitoring of the time course of biodistribution The data set on ... %/420.6 number of events 103 103 C B 70.3 %/252.2 101 102 102 A 100 101 B 0 number of events 100 100 128 128 Fig Semilogarithmic representation of the fluorescent surface staining of cells of the human ... tendency for enhanced binding relative to the core-fucosylated N-glycan indicated that the new branch is not an inert modification at this level of testing The decrease of cell positivity for KG-1 cells...

Ngày tải lên: 30/03/2014, 16:20

... constructed uniformly and consisted of three sets of ten digits specifying addresses E1(l), E1 (2), E1(3) (See Fig 4.) Program for MT 57 dress E1(1) = S, the address of the initial instruction of a routine ... addresses of the first equivalents altered to the address of the initial order of the new routine The size of storage in the computer imposed severe limits on the extent and performance of the program ... particular part of the program Secondly, the method of effecting change of word order appears to be applicable only to subsections of languages where permutation of target language order into foreign...

Ngày tải lên: 30/03/2014, 17:20

... treated cells +/- inactivated serum Cells negative for both Annexin V and PI were deemed viable cells Complement-mediated Cytotoxicity of Whole Ascites A single cell suspension of whole ascites cells ... isolate single cells for FACS For Alemtuzumab induced cytotoxicity assays in whole ascites, after red cell lysis, whole cell pellets were resuspended in 1/20th of the original volume of ascites supernatant ... myeloid cells appear to be a primary source of resistance to antiVEGF therapy, suggesting a critical role for these cells in tumor angiogenesis [5] The exact mechanism of action of myeloid cells...

Ngày tải lên: 18/06/2014, 15:20

... regulatory T cells (T reg) cells [2] These cells constitute 1-10% of * Correspondence: ciro.piccirillo@mcgill.ca † Contributed equally FOCIS Center of Excellence, Research Institute of the McGill ... Hence miRNA155 is required for Treg cell fitness in contexts of differential IL-2 levels in contexts of homeostasis and inflammation Therefore, the waning of Treg cells, and ensuing breakdown ... development is independent of IL-2, while this cytokine is essential for survival of Treg cells [43] Moreover, although IL-2-/- or IL-2R-/mice display reduced numbers of Treg cells in vivo, their suppressive...

Ngày tải lên: 18/06/2014, 16:20

... at 95°C for min, and then 30 cycles at 94°C for 45 s, 54°C for min, 72°C for and a final extension cycle of 72°C for The house-keeping gene b-actin was taken as a loading control HEK293T cells ... Ana-1 and HUVEC cells was homoplastic with that on OS-RC-2 cells As shown in Figure 1C, the toxicity profiling of PCFC-g-PEI on HUVEC cells was similar with that on OS-RC-2 cells For example, with ... FA-PEAs are amphiphilic polymers, both of them may form polymeric micelles through self-assembling During the process of the micelle formation, the concentrations of polymers in solutions would be...

Ngày tải lên: 18/06/2014, 19:20

... at 95°C for min, and then 30 cycles at 94°C for 45 s, 54°C for min, 72°C for and a final extension cycle of 72°C for The house-keeping gene b-actin was taken as a loading control HEK293T cells ... Ana-1 and HUVEC cells was homoplastic with that on OS-RC-2 cells As shown in Figure 1C, the toxicity profiling of PCFC-g-PEI on HUVEC cells was similar with that on OS-RC-2 cells For example, with ... FA-PEAs are amphiphilic polymers, both of them may form polymeric micelles through self-assembling During the process of the micelle formation, the concentrations of polymers in solutions would be...

Ngày tải lên: 20/06/2014, 03:20

... relevance of the curriculum content and didactic quality of information delivered to journalists (process evaluation) and, b) explore the effects of such programs on reporting of HIV/AIDS related information ... assessment of the content of each J2J program follows: Barcelona 2002 J2J program This program was graded as fairly complete by our team of evaluators The agenda allowed ample time for discussion ... that his/her program was broadcast monthly Examples of Experiences of Journalists Two sources of descriptive evaluations of the program were available A database from the J2J Sydney program and...

Ngày tải lên: 20/06/2014, 08:20