7physiological roles of nf kappa b in innate immunity

Tài liệu Báo cáo khoa học: Autophagy inhibits reactive oxygen species-mediated apoptosis via activating p38-nuclear factor-kappa B survival pathways in oridonin-treated murine ﬁbrosarcoma L929 cells doc

... Oridonin treatment led to a decreased inhibitor kappa B (I-jB) level, with the increase of phosphorylated I-jB (p-I-jB) and NF- jB levels being time-dependently indicative of NF- jB activation Interestingly, ... in oridonin-induced L929 cells Interestingly, we found that inhibition of p38 decreased oridonin-induced NF- jB activation, indicating that oridonin-induced p38 activation contributes to NF- jB ... NF- jB by PDTC or siRNA increased the oridonin-induced SubG1 cell proportion Additionally, oridonin induced a decrease of I-jB levels but an increase of p-I-jB and NF- jB levels These results indicate...

Ngày tải lên: 18/02/2014, 13:20

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Tài liệu Báo cáo khoa học: Staphylococcal enterotoxin C1-induced pyrogenic cytokine production in human peripheral blood mononuclear cells is mediated by NADPH oxidase and nuclear factor-kappa B doc

... trigger the production of IL- 1b and IL-6 that is blocked by the NF- jB inhibitor (PDTC or SN-50) Moreover, the NF- jB binding ability is abrogated by binding site competition and antibody supershift analysis ... Decursin inhibits induction of in ammatory mediators by blocking NF- jB activation in macrophages Mol Pharmacol 69, 1783–1790 16 Laos S, Baeckstrom D & Hansson GC (2006) Inhibition of NF- kappaB activation ... Primary antibodies including mouse monoclonal NF- jB p65, rabbit polyclonal NF- jB p65, goat and rabbit polyclonal NF- jB p50, and rabbit polyclonal p47phox were obtained from Santa Cruz Biotechnology...

Ngày tải lên: 19/02/2014, 00:20

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Báo cáo y học: "The S100A8/A9 heterodimer amplifies proinflammatory cytokine production by macrophages via activation of nuclear factor kappa B and p38 mitogen-activated protein kinase in rheumatoid arthritis" potx

... specificity of NF- B protein binding was verified by inhibition and supershift experiments with unlabeled NF- B consensus or mutant oligonucleotides and anti -NF- B p65 antibody (b) Effects of p38 MAPK inhibition ... MAPK inhibitors did not reduce the DNA-binding activity of NF- B, these two critical signaling pathways may be independently involved in S100A8/A9-mediated cytokine production NF- B inhibitors ... proteins were measured for the DNA-binding activity of NF- B by EMSA As shown in Figure 7a, the NF- B activity was induced by S100A8/A9 in a dose-dependent manner, verifying the activation of NF- κB...

Ngày tải lên: 09/08/2014, 07:20

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Báo cáo y học: " Nontypeable Haemophilus influenzae induces COX-2 and PGE2 expression in lung epithelial cells via activation of p38 MAPK and NF-kappa B" pps

... NTHi-induced NF- kappa B signaling pathway EMSA analysis showed that NFkappa B translocation and its DNA binding activity were markedly increased by NTHi within 90 mins, and DNA binding activity of NF- kappa ... binding activity of NF- kappa B induced by NTHi A549 cells were incubated with different concentrations of NTHi with or without SB203580, and DNA binding activity of NF- kappa B was measured by ... of MAPK inhibited NF- kappa B- dependent gene transcription without affecting IkappaBalpha phosphorylation and NF- kappa B- DNA binding ability [9,2427] In our study, the fold induction of NTHi-induced...

Ngày tải lên: 12/08/2014, 15:21

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Báo cáo y học: "Potential role and mechanism of IFN-gamma inducible protein-10 on receptor activator of nuclear factor kappa-B ligand (RANKL) expression in rheumatoid arthritis" pptx

... the synovium of inflamed joints and thus mediate inflammation [10,11], the etiology of RA remains unknown A Th1/Th2 cytokine imbalance with a predominance of Th1 cytokines, including IFN-g, is ... Gai activation by ADP-ribosylating Gai subunits, and the inhibition of Gai by PTX was confirmed by measuring CREB phosphorylation that was increased following Gai inhibition Figure shows that ... lysed in a lysis buffer (Cell Signaling Technology, Inc., Danvers, MA, USA) by incubating the suspension on ice for 20 minutes The protein concentration of the lysate was measured using the bicinchoninic...

Ngày tải lên: 12/08/2014, 17:22

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Tài liệu Báo cáo khoa học: Polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells pdf

... different binding partners including ezrin that can be anchored to the actin cytoskeleton [24] The potential contribution of ezrin in apical distribution of iNOS is inferred from the observation ... enrichment in iNOS activity within the particulate fraction of epithelial cells Apical distribution of iNOS in intestinal epithelial cells To get more insight into the localization of iNOS in intestinal ... up-regulated in mice exposed to ﬂagellin (Fig 5B) Immunostaining of duodenum sections revealed that iNOS was distributed apically in B A B Fig iNOS localizes to the apical domain of polarized intestinal...

Ngày tải lên: 19/02/2014, 16:20

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Báo cáo khóa học: Disruption of the interaction between the Rieske iron–sulfur protein and cytochrome b in the yeast bc1 complex owing to a human disease-associated mutation within cytochrome b potx

... (as shown in Fig 4B) The inhibition of the enzyme by its product quinone could be excluded, because the addition of an equal amount of decylubiquinone to decylubiquinol at the beginning of the assay ... is discussed in greater detail below Instability of the mutant enzyme Further analysis of the kinetic parameters of the G167E mutant was hindered by rapid inhibition of the activity of Fig QH2-cytochrome ... described below Stigmatellin was purchased from Fluka Preparation of decylubiquinol Ten milligrams of 2,3-dimethoxy-5-methyl-n-decyl-1,4-benzoquinone (decylubiquinone), an analogue of ubiquinone...

Ngày tải lên: 07/03/2014, 15:20

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Báo cáo khoa học: Interaction of human steﬁn B in the preﬁbrillar oligomeric form with membranes Correlation with cellular toxicity doc

... binding of the aggregated forms, albeit to a much smaller extent than that observed for some proteins that bind specifically to membranes, such as the small membrane-binding domains involved in ... chip by three injections of 100 mm NaOH Unspecific binding sites were blocked by one injection of 0.1 mgÆmL)1 bovine serum albumin For the binding experiment proteins were injected at lm concentration ... evaluation of polyethyleneimine precipitation in isolation of recombinant cysteine proteinase inhibitor stefin B Protein Expr Purif 5, 65–69 49 MacDonald RC, MacDonald RI, Menco BP, Takeshita K, Subbarao...

Ngày tải lên: 07/03/2014, 17:20

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Báo cáo khoa học: Regulation of DNp63a by tumor necrosis factor-a in epithelial homeostasis docx

... performed as in (B) except for the use of a different NF- jB inhibitor, BAY 11-1082 (10 lM) The same blot was reprobed for western blot analysis with anti-laminA ⁄ C as loading control NF- jB inhibitors ... occurred after combined treatment with TNF-a and CHX, and was blocked by the NF- jB inhibitor JSH23 The same blot was reprobed with antibodies to b- actin as loading control (B) ME180 cells grown ... TNF-a destabilizes DNp63a by both proteasomal and caspase-dependent degradation pathways The degradation of DNp63a by TNF-a was attenuated by inhibition of NF- jB, suggesting that activation of...

Ngày tải lên: 16/03/2014, 06:20

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Báo cáo khoa học: Transgenic Cdx2 induces endogenous Cdx1 in intestinal metaplasia of Cdx2-transgenic mouse stomach pot

... Thomasset M, Traber PG & Perret C (1996) cis-Acting elements and transcription factors involved in the intestinal specific expression of the rat calbindin-D9K gene: binding of the intestinespecific ... 2009 FEBS H Mutoh et al Cdx1 expression in intestinal metaplasia A Intron Cdx2 stomach CpG, indicated by the box in Fig 3B, that was methylated in five normal intestines and five intestinal metaplasias ... expressed in Cdx2-induced intestinal metaplasia was investigated Endogenous Cdx1 was detected in the normal intestine and in all of the Cdx2-induced intestinal metaplasia, but not in the normal...

Ngày tải lên: 23/03/2014, 04:20

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Báo cáo khoa học: Transport of the phosphonodipeptide alafosfalin by the H+/peptide cotransporters PEPT1 and PEPT2 in intestinal and renal epithelial cells doc

... Mechanism of intestinal absorption and renal reabsorption of an orally active ace inhibitor: uptake and transport of fosinopril in cell cultures Drug Metab Dispos 29, 1307–1315 Ó FEBS 2004 Alafosfalin ... 580 lM in the absence of the inhibiting phosphonodipeptide The same alafosfalin effect was observed in SKPT cells where the Vmax values were 2.2 ± 0.1 nmolÆ(10 min)1)Æ(mg protein))1 in the absence ... Technologies, Inc (Karlsruhe, Germany) Fetal bovine serum was obtained from Biochrom (Berlin, Germany) Insulin, dexamethasone and glycylsarcosine (Gly-Sar) were from Sigma Chemie (Deisenhofen, Germany)...

Ngày tải lên: 23/03/2014, 12:20

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Báo cáo khoa học: Nuclear factor kappa B and tumor necrosis factor-alpha modulation of transcription of the mouse testis- and pre-implantation development-speciﬁc Rnf33⁄Trim60 gene pot

... positive-acting kappa B (jB) element that was located in the single intron of the Rnf33 gene adjacent to exon 1; the intronic jB was targeted by the p65 ⁄ RelA and p50 ⁄ NFjB1 nuclear factor-jB (NF- jB) ... targeted by the NF- jB proteins p50 and p65 It has been well established that jB sequences are targeted by the abundantly expressed NF- jB [13–15] Involvement of the NF- jB in gene regulation in the ... indicate that the NF- jB subunit proteins p65, and possibly p50, target the intronic jB site of Rnf33, resulting in transcriptional activation of Rnf33 in the testis The p65 protein seems to be...

Ngày tải lên: 29/03/2014, 00:20

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Báo cáo khoa học: Effect of 5-lipoxygenase inhibitor MK591 on early molecular and signaling events induced by staphylococcal enterotoxin B in human peripheral blood mononuclear cells doc

... lipoxygenase inhibitors (MK886, MK591 and curcumin), p38kinase inhibitor (SB203580), JNK inhibitor (SP600125) and MAPK inhibitor (PD98059) to observe the effect in human PBMCs As all investigated protein ... of SEB-induced cascades in human PBMCs have not been examined in detail To gather evidence vital in identifying early signaling events triggered by super-antigen SEB in lethal shock, we examined ... labeled thymidine incorporation (in cpm) The investigation then focused on analyzing the effect of the same set of inhibitors on TNF-a induction (Table 1) The observed induction in SEB treated cells...

Ngày tải lên: 30/03/2014, 04:20

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Báo cáo sinh học: " Open Access Cloning of the canine RNA polymerase I promoter and establishment of reverse genetics for influenza A and B in MDCK cells" docx

... against the development of a full blown pandemic To this end, MDCK cells are being developed by many of the influenza vaccine manufacturers as a cell-substrate for influenza vaccine production because ... used in the rescue of recombinant influenza will function only in primate or avian cells [5-7] In order to rescue influenza using the RNA pol I transcription machinery in canine cells, cloning ... BamHI BamHI BamHI AvrII SpeI EcoRI SpeI HindIII SphI XbaI XbaI BamHI * XbaI BamHI BamHI SacI BamHI * EcoRI BamHI SacI BamHI BamHI AvrII 18S rRNA Gene MDCK Eco RI 7.1 kb Fragment B M C M Probe C C...

Ngày tải lên: 18/06/2014, 18:20

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Báo cáo sinh học: " Improvement of cardiac contractile function by peptide-based inhibition of NF-B in the utrophin/dystrophin-deficient murine model of muscular dystrophy" potx

... efficacy of the NBD peptide in cardiac muscles of dko treated mice Both NF- B DNA binding activity, as well as nuclear levels of the p65 subunit of NF- B were elevated in the dko heart In general, ... large fibrotic scars (Figure 6A) in the hearts of most of dko mice in this study regardless of treatment (5 of [71%] NBD treated mice, versus of Figure NBD is effective in inhibiting NF- B in cardiac ... Li ZW, Beg AA, Ghosh S, Sahenk Z, Weinstein M, Gardner KL, RafaelFortney JA, Karin M, Tidball JG, Baldwin AS, Guttridge DC: Interplay of IKK/ NF- kappaB signaling in macrophages and myofibers promotes...

Ngày tải lên: 18/06/2014, 19:20

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Báo cáo hóa học: "Urinary N-acetyl-beta -D-glucosaminidase and its isoenzymes A & B in workers exposed to cadmium at cadmium plating" pot

... Urine cadmium (μg/g of creatinine) Urinary Total NAG (U/g of creatinnine) Urinary NAG-A (U/g of creatinnine) Urinary NAG -B (U/g of creatinnine) Urine cadmium Urinary Total NAG Urinary NAG-A Urinary ... cadmium plating process shown the nasal toxicity and renal tubular dysfunction by using urinary β2microglobulin [4-6] The urinary β2-microglobulin is unstable in acidic urine The levels of urinary ... significant influence (53%) on urinary total-N-acetylbeta -D-glucosaminidase activity Subjects who had urinary Cd levels greater than μg/g of creatinine appeared to have an influence (52%) on urinary...

Ngày tải lên: 20/06/2014, 00:20

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Báo cáo hóa học: " Cloning of the canine RNA polymerase I promoter and establishment of reverse genetics for influenza A and B in MDCK cells" pdf

... against the development of a full blown pandemic To this end, MDCK cells are being developed by many of the influenza vaccine manufacturers as a cell-substrate for influenza vaccine production because ... used in the rescue of recombinant influenza will function only in primate or avian cells [5-7] In order to rescue influenza using the RNA pol I transcription machinery in canine cells, cloning ... BamHI BamHI BamHI AvrII SpeI EcoRI SpeI HindIII SphI XbaI XbaI BamHI * XbaI BamHI BamHI SacI BamHI * EcoRI BamHI SacI BamHI BamHI AvrII 18S rRNA Gene MDCK Eco RI 7.1 kb Fragment B M C M Probe C C...

Ngày tải lên: 20/06/2014, 01:20

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báo cáo hóa học:" Improvement of cardiac contractile function by peptide-based inhibition of NF-B in the utrophin/dystrophin-deficient murine model of muscular dystrophy" doc

... efficacy of the NBD peptide in cardiac muscles of dko treated mice Both NF- B DNA binding activity, as well as nuclear levels of the p65 subunit of NF- B were elevated in the dko heart In general, ... large fibrotic scars (Figure 6A) in the hearts of most of dko mice in this study regardless of treatment (5 of [71%] NBD treated mice, versus of Figure NBD is effective in inhibiting NF- B in cardiac ... Li ZW, Beg AA, Ghosh S, Sahenk Z, Weinstein M, Gardner KL, RafaelFortney JA, Karin M, Tidball JG, Baldwin AS, Guttridge DC: Interplay of IKK/ NF- kappaB signaling in macrophages and myofibers promotes...

Ngày tải lên: 20/06/2014, 03:20

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báo cáo hóa học:" Substitutions in the Reverse Transcriptase and Protease Genes of HIV-1 Subtype B in Untreated Individuals and Patients Treated With Antiretroviral Drugs" potx

... synthesis of the negative strand of DNA can result in the insertion of a uracil as a result of the deamination of a cytosine, leading to the inclusion of an adenosine instead of guanosine in positive-stranded ... H, Wainberg M-A, Brenner B, et al.: Prevalence of HIV-1 resistant to antiretroviral drugs in 81 individuals newly infected by sexual contact or injecting drug use Investigators of the Quebec Primary ... hypermutation may involve a cellular factor, APOBEC3G, a cytidine deaminase that converts cytosine to uracil The activity of APOBEC3G is inhibited by the Vif protein.[5,7,8] In the absence of Vif, the...

Ngày tải lên: 20/06/2014, 08:20

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