6microbial modulation of nf kappa b signaling commensals and pathogens

Báo cáo y học: " Nontypeable Haemophilus influenzae induces COX-2 and PGE2 expression in lung epithelial cells via activation of p38 MAPK and NF-kappa B" pps

... activity of NF- kappa B induced by NTHi A549 cells were incubated with different concentrations of NTHi with or without SB203580, and DNA binding activity of NF- kappa B was measured by EMSA analysis ... within 90 mins, and DNA binding activity of NF- kappa B was not affected by pretreatment of specific p38 MAPK inhibitor SB203580 These data indicate that p38 MAPK and NF- kappa B are two independent ... shown in Fig 6A & 6B, NTHi-induced COX-2 mRNA expression was markedly inhibited by pre-treatment of NF- kappa B inhibitor PDTC and p38 MAPK inhibitors SB203580 and Page of (page number not for citation...

Ngày tải lên: 12/08/2014, 15:21

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báo cáo hóa học: " Inhibition of NF-κB activation by 5-lipoxygenase inhibitors protects brain against injury in a rat model of focal cerebral ischemia" docx

... effect of BW -B 70C on the activation of NF- B was examined in BV2 cells BW -B 70C inhibited the LPS-mediated NF- κBdependent luciferase activity in a dose-dependent manner (Fig 5A) To confirm further ... (B) Effect of BW -B 70C on infarct volume (measured in six serial coronal sections arranged from cranial to caudal regions), (C) Effect of BW -B 70C on neurological score and (D) Effect of BWB ... both 5-LOX and NF- B It is a potent and a selective inhibitor of 5-LOX in vitro and in vivo with a long half-life and high oral bioavailability Other potent 5-LOX inhibitors, caffeic acid and 2,...

Ngày tải lên: 19/06/2014, 22:20

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Báo cáo y học: "κ Increased AP-1 and NF-κB activation and recruitment with the β combination of the proinflammatory cytokines IL-1β, tumor necrosis factor alpha and IL-17 in rheumatoid synoviocyte" ppt

... low concentrations of IL-1β and of IL-17 showed a synergistic effect on the nuclear translocation of C-Fos, of FosB, of Fra-1, of Fra-2, of C-Jun, of JunD and of NF- B This combination had a particular ... almost all of the TFs Similarly, in myofibroblasts, IL-6 production was enhanced by IL-17 combined with IL-1β and TNF-α through an activation of NF- B [23,24] The effects of IL-17 with TNF-α were ... translocation of Fra-1, of C-Jun and of NF- B Available online http://arthritis-research.com/content/6/3/R190 Figure Effects of IL-1β, tumor necrosis factor alpha (TNF-α) and IL-17 used alone and in combination...

Ngày tải lên: 09/08/2014, 01:23

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Báo cáo y học: "The S100A8/A9 heterodimer amplifies proinflammatory cytokine production by macrophages via activation of nuclear factor kappa B and p38 mitogen-activated protein kinase in rheumatoid arthritis" potx

... factor kappa B (NF- B) pathway, was identified as a functional receptor for the S100A12 protein [14] Structural similarities between S100A12 and other S100 proteins [3] and the binding of S10 0B and ... with 100 ng/ml of lipopolysaccharide were used as positive controls The specificity of NF- B protein binding was verified by inhibition and supershift experiments with unlabeled NF- B consensus ... oligonucleotides and anti -NF- B p65 antibody (b) Effects of p38 MAPK inhibition on S100A8/A9-induced NF- B activation Monocytes, after two-hour pretreatment with or without the MAPK inhibitors (PD98059, SB202190,...

Ngày tải lên: 09/08/2014, 07:20

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Báo cáo y học: "Elevated expression of CD30 in adult T-cell leukemia cell lines: possible role in constitutive NF-κB activation" pps

... IκBs Activation of NF- B generally involves phosphorylation and degradation of IκBs, followed by nuclear translocation of NF- B dimers and subsequent activation of the genes containing NF- B binding ... Alternatively, NF- B activation occurs by inducible processing of NFKB2/p100 with I B- like inhibitory activity, into p52 with DNA binding activity, followed by nuclear translocation of p52 containing NF- B ... tax-independent NF- B activation, such as a gain of function of the NF- B activating molecule(s) or a loss of function of the NF- B regulator(s) The elucidation of the molecular mechanism of NF- B activation...

Ngày tải lên: 13/08/2014, 09:21

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Tài liệu Báo cáo khoa học: Autophagy inhibits reactive oxygen species-mediated apoptosis via activating p38-nuclear factor-kappa B survival pathways in oridonin-treated murine ﬁbrosarcoma L929 cells doc

... inhibitor kappa B (I-jB) level, with the increase of phosphorylated I-jB (p-I-jB) and NF- jB levels being time-dependently indicative of NF- jB activation Interestingly, we found that inhibition of ... cells, NF- jB is sequestered in the cytoplasm by binding to the inhibitor of NF- jB (I-jB) In response to a variety of stimuli, activation of NF- jB typically involves the phosphorylation of I-jB, resulting ... we found that inhibition of NF- jB activation by using NF- jB inhibitor pyrrolidine dithiocarbamate (PDTC) or speciﬁc siRNA, which caused a reduction in NF- jBp65 levels (Fig 6B) , signiﬁcantly increased...

Ngày tải lên: 18/02/2014, 13:20

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Tài liệu Báo cáo khoa học: Staphylococcal enterotoxin C1-induced pyrogenic cytokine production in human peripheral blood mononuclear cells is mediated by NADPH oxidase and nuclear factor-kappa B doc

... attenuates the movement of p47phox (Fig 5B) , translocation of NF- jB (Fig 2C), DNA-binding activity of NF- jB (Fig 2D) and phosphorylation of IKK -b, IjBa (Fig 4), whereas inhibitors of NF- jB not affect the ... translocation of NF- jB which may bind to its target genes to trigger the production of IL- 1b and IL-6 that is blocked by the NF- jB inhibitor (PDTC or SN-50) Moreover, the NF- jB binding ability is abrogated ... Primary antibodies including mouse monoclonal NF- jB p65, rabbit polyclonal NF- jB p65, goat and rabbit polyclonal NF- jB p50, and rabbit polyclonal p47phox were obtained from Santa Cruz Biotechnology...

Ngày tải lên: 19/02/2014, 00:20

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Báo cáo y học: "DNA-like class R inhibitory oligonucleotides (INH-ODNs) preferentially block autoantigen-induced B-cell and dendritic cell activation in vitro and autoantibody production in lupus-prone MRL-Faslpr/lpr mice in vivo" potx

... activation of macrophages, DCs, Page of 16 (page number not for citation purposes) AM14 B cells, and primary mouse B cells Figures 6a and 6b show the ability of both classes of INH-ODNs to block the ... isothiocyanate class B INH-ODNs as inhibitors of both anti-dsDNA antibody secretion (detected by immunofluorescence and by ELISA) and of anti-Sm/RNP antibody secretion (as detected by ELISA) (Figure ... IFN-α mAb (22100-1; PBL Biomedical Laboratories, Piscataway, NJ, USA), rabbit anti-mouse IFN-α polyclonal antibody (32100-1; PBL Biomedical Laboratories), HRP-conjugated donkey anti-rabbit IgG...

Ngày tải lên: 09/08/2014, 14:21

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báo cáo khoa học: "HGF/c-Met related activation of b-catenin in hepatoblastoma" pps

... http://www.jeccr.com/content/30/1/96 Page of 10 Figure Immunohistochemical staining of HB using an antibody to b- catenin (a) Cytoplasmic staining of b- catenin in hepatoblastoma (b) Nuclear and cytoplasmic accumulation of b- catenin ... that aberrant activation of bcatenin may be critical to the pathogenesis of HB but the means of this activation may not be as important as was previously thought Our finding of a large number of ... subtype, b- catenin and Y654 b- catenin IHC and CTNNB1 gene status of hepatoblastomas with mutations Case Number Histologic Type Histologic Subtype b- catenin Y654 -b- catenin CTNNB1 mutation CCRG9...

Ngày tải lên: 10/08/2014, 10:21

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Báo cáo y học: "Early Inhibition of NF-κB activation and MMP-9 secretion by plasma of human volunteers after ingestion of maritime pine bark extract (Pycnogenol)" pot

... Inhibition bark extract after five days intakeby plasma of five Inhibition of LPS-induced NF- B activation by plasma of five volunteers before and after five days intake of 200 mg maritime pine bark ... 10 15 20 25 30 Inhibition of NF- kB activation (%) Figure teers before and after matching plasma and MMP-9 of inhibition of LPS-induced NF- B of five volunCorrelation release by five days intake ... we observed a positive correlation between inhibitory activity of matched plasma samples on MMP-9 and NF- B In vitro inhibition of NF- B activation by plant extracts or constituents has been...

Ngày tải lên: 11/08/2014, 08:21

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Báo cáo y học: "Dysregulated apoptosis and NFκB expression in COPD subjects" pptx

... Sonenshein GE: Inhibition of NF- kappaB/ Rel induces apoptosis of murine B cells EMBO J 1996, 15:4682-4690 Grimm S, Bauer MK, Baeuerle PA, Schulze-Osthoff K: Bcl-2 downregulates the activity of transcription ... concentrations of sFas-L, an inducer of apoptosis, and sFas, an inhibitor of apoptosis, were measured using commercially available ELISA assays (IDS Ltd UK) Quantification of NF B activation and I B phosphorylation ... nuclear I B is associated with inhibition of NF B activation and the subsequent induction of apoptosis [42] In bronchial epithelium, there is an increase in p65 expression (regarded as NF B activation)...

Ngày tải lên: 12/08/2014, 14:20

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Báo cáo y học: "Potential role and mechanism of IFN-gamma inducible protein-10 on receptor activator of nuclear factor kappa-B ligand (RANKL) expression in rheumatoid arthritis" pptx

... expression, we used PTX, a bacterial toxin that inhibits Gai activation by ADP-ribosylating Gai subunits, and the inhibition of Gai by PTX was confirmed by measuring CREB phosphorylation that was ... membrane was subsequently washed and incubated with a peroxidase-labeled rabbit anti-goat IgG antibody for hours at room temperature and then incubated with an enhanced chemiluminescence substrate ... expression of RANKL in human RA synoviocytes and CD4+ T cells and to find which subunit of CXCR3 contributes to RANKL expression by CXCL10 Materials and methods Synovial fluid and sera Synovial fluid and...

Ngày tải lên: 12/08/2014, 17:22

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Environmental and Social Impacts of Shrimp farming in Tam Giang lagoon, Vietnam-Local perception

... CONTENTS Acknowledgements Abstract List of Abbreviations and Acronyms Table of Contents List of Tables List of Figures INTRODUCTION 1.1 Objectives of the study 10 1.2 Research ... been the destruction of mangroves and other wetlands for the construction of shrimp ponds More than 50% of the world’s mangroves have been removed, and the establishment of shrimp ponds has been ... vectors and therefore become secondary victims of infection In addition, birds also eat the infected shrimp and fly to the other farms and might be dangerous carriers In this way, outbreaks of disease,...

Ngày tải lên: 02/11/2012, 10:15

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B-to-B Marketing in 2009:Trends in Strategies and Spending

... 2009, and about their attitudes and beliefs regarding the effectiveness of various tactics for generating leads and building brand awareness As shown on the following pages, there are a number of ... Spending Table of Contents Respondent Profile Current Marketing Tactics and Budget Allocations 14 Attitudinal and Behavioral Analysis 29 B- to -B Marketing in 2009: Trends in Strategies and Spending ... the 95% level of confidence: A /B 13 Not in a marketing role B- to -B Marketing in 2009: Trends in Strategies and Spending CURRENT MARKETING TACTICS AND BUDGET ALLOCATIONS 14 B- to -B Marketing in...

Ngày tải lên: 10/08/2013, 16:17

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ĐỀ THI KHẢO SÁT ĐẠI HỌC MÔN TOÁN KHỐI (A,B,D) - TRUNG TÂM LUYỆN THI _NGUYỄN TRUNG TRỰC

... CÂU VI : Cho tam giác cân ABC B có tung độ B khác -3.đỉnh A (-3,-3) đường tròn nội tiếp tam giác ABC có phương trình (x-1)2 + y2 =9 Tìm B CÂU VII: Cho ba điểm A(-1,-2,0) B( 3,1,2) C(1,0,1) mặt phẳng ... phẳng P cho b n điểm A ,B, C,D đồng phẳng Và b n điểm hình thang CÂU IX: Tìm số phức z biết z(1-2i) =(3+4i)(2-i)2 B I LÀM THÊM tinh tích fân sau : : d(x) ∫ (x31+1) 3 √1+x CHÚC CÁC EM LÀM B I TỐT ...

Ngày tải lên: 05/09/2013, 16:34

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Tài liệu ĐỀ THI THỬ ĐH-CĐ 2010 MÔN TOÁN KHỐI A–B–D–V_TRUNG TÂM THÀNH ĐẠT(ĐỀ 02) pptx

... VMCNB MC MN MB 6 ã Vỡ D l trung im ca MC nờn d ( M ,(CNB)) = 2d (D ,(CNB)) ị VMCNB = 2VDCNB = VDCSB = VS ABCD ị VDPQCNB = VSABNPQ 7 VS ABCD ị VSABNPQ = VS ABCD ị = 12 12 VDPQCNB Cõu V: T gi ... Ax + By + Cz = (vi A2 + B + C ) ã Vỡ (P) ^ (Q) nờn: A + 1 .B + 1.C = C = - A - B (1) A + 2B - C ã d ( M ,( P )) = 2 A +B +C = ( A + B - C )2 = 2( A2 + B2 + C ) (2) B = (3) (4) ã T (3): B = ... c: B = 2p -2 ã Tớnh B = x - x dx = A + B ũ ã Tớnh A = = -2 Cõu IV: Gi P = MN ầ SD, Q = BM ầ AD ị P l trng tõm DSCM, Q l trung im ca MB ã VMDPQ VMCNB = MD MP MQ 1 = = ị VDPQCNB = VMCNB MC...

Ngày tải lên: 23/12/2013, 08:17

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Tài liệu Báo cáo khoa học: Collagen I regulates matrix metalloproteinase-2 activation in osteosarcoma cells independent of S100A4 pdf

... Fig The expression of MT1-MMP and TIMP-2 in pHb-1 and II1 1b cells Determination of MT1-MMP (A) and TIMP-2 (B) by western blot of cell lysates from pHb-1 and II-1 1b cells (A) Cells were attached ... Expression of S100A4 in pHb-1 and II-1 1b cells (A) Determination of S100A4 by western blotting of cell lysates from pHb-1 and II-1 1b cells attached to plastic, using a total protein concentration of ... USA), S100A4 rabbit polyclonal from Abcam (Cambridge, UK) and RECK mouse mAb from BD Biosciences (San Jose, CA, USA) Anti- mouse and anti-rabbit IgG horseradish peroxidase-linked antibodies were...

Ngày tải lên: 18/02/2014, 11:20

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Tài liệu Báo cáo Y học: Granule-bound starch synthase I A major enzyme involved in the biogenesis of B-crystallites in starch granules ppt

... CD142) with an insert of 1696 bp was isolated and fully sequenced on both strands and submitted to GenBank (accession number AF026420) To obtain more information about the 5¢ end of this cDNA, an ... Chlamydomonas GBSSI in the same set of experiments Because of this high activity, we were able to double the amount of polysaccharide by a 24 h incubation of puriﬁed starch granules in the presence of 3.2 ... in GenBank (accession number AF433156) Figure displays the length and position of the six introns within the GBSSI sequence compared with those of rice and potato The number and position of the...

Ngày tải lên: 22/02/2014, 07:20

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