6combinatorial transcription factor action and epigenetic modification

... Ets factors may direct the choice of the promoter elements in play, and therefore, determine the selective combination of transcription factors involved and the regulatory pathways modulating transcription ... The mechanism of action of sumoylation of ERM is unknown and could potentially involve the regulation of interactions with cofactors We have identified an interaction between CHD3 and the CIDD region ... assays also showed interactions between SUMO-1 and p53 and p73 [39] Hence it is possible that CHD3 is implicated in complexes with p73 and p53 through interaction with SUMO-1 (and in yeast the SUMO-1...

Ngày tải lên: 30/03/2014, 09:20

... cultures in normal oxygen the gene expression of collagen types II, IX, X and XI and the transcription factors SOX6 and SOX9 was greatly increased (p < 0.05 or p < 0.001) in comparison with monolayer ... lowered oxygen tension, and this was correlated with the selective upregulation of HIF2α and increased expression of the key chondrogenic transcription factors SOX9, SOX5 and SOX6 Immunohistochemistry ... expression for hypoxia-inducible transcription factors (HIF) and cultures of infrapatellar fat pad cells SOX genes (a), collagens (b) and proteoglycans (c) in monolayer culture and after chondrogenic differentiation...

Ngày tải lên: 09/08/2014, 10:20

... realistic model for competitive interactions into GOMER (see Materials and methods) and have used this model to study the interaction of yeast transcription factors Ndt80p and Sum1p Ndt80p is an activator ... close and precise spacing in the cooperative interaction of Fkh2p and Mcm1p [17] One possibility is that Fkh2p and Mcm1 bind cooperatively by two different mechanisms: through direct interaction ... closely match the specificity of that particular transcription factor and are, therefore, less sensitive to the effects of the competing factor Fraction of Rap1p bound http://genomebiology.com/2005/6/10/R87...

Ngày tải lên: 14/08/2014, 14:22

... 67 xi ABBREVIATIONS ATF activating transcription factor ATF4 activating transcription factor ATF5 activating transcription factor ATF6 activating transcription factor bZIP basic zipper CHOP C/EBP ... recombinant ATF5 and ATF4 protein, respectively, and the specific transcription factor in mouse and human cell lines that was induced by different stress conditions Cell Culture and Stress Conditions ... REGULATING THE TRANSCRIPTION FACTOR ATF5 AND ITS FUNCTION IN THE INTEGRATED STRESS RESPONSE Phosphorylation of eukaryotic initiation factor (eIF2) is an important mechanism regulating global and gene-specific...

Ngày tải lên: 24/08/2014, 11:17

... the zero, xN and 0.87xH frequencies, versus the sequence of the TTF-1 HD Measurements were done at 11.7 T and 286 K Blank slots are for residues 29 and 42 (prolines) and Glu17, Leu34 and Lys46, ... (9.84 ± 0.20 ns) and two for sgi (0.26 ± 0.03 ns and 0.55 ± 0.06 ns) (supplementary Doc S1 and Fig S2) Comparison of results from MF and RSDM The results for sm obtained by the MF and RSDM approaches ... when the HD is part of a much larger transcription factor, and determine the extent of the conformational changes and, hence, the energetics of the interaction with DNA [2,3,8,11,32] Experimental...

Ngày tải lên: 18/02/2014, 16:20

... family and CRE binding 6428 factors involved in the NFjB pathway and other functional related factors, such as p300 and CBP Group D contain factors related to cell cycle and DNA repair-related factors ... TAFII and NC2 and NF-Y factors that have histone fold motifs [52] Group G is a small subset that contains all the SMAD proteins of the HTFN and APC and b-catenin-related factors Groups C and D ... 2005 FEBS C Rodriguez-Caso et al Human transcription factor network topology Table Description and functionality of transcriptions factor hubs Transcription factor (TF), degree (k), betweenness...

Ngày tải lên: 19/02/2014, 07:20

... AMP, and GTP) and 2-OG at different concentrations on GlnK binding to TnrA GlnK–TnrA interaction in the presence of mm ATP On the other hand, 2-oxoglutarate did not influence the GlnK–TnrA interaction, ... exponential growth phase, and the cells were then washed and resuspended in SMM without nitrate, and finally incubated for a further 20 Samples were taken before and after the shift, and soluble cell-free ... assimilatory enzymes glutamine synthetase (GS) and glutamate synthase, respectively [6–8] TnrA belongs to the MerR family of transcription factors, and is present as a homodimer of two 12-kDa subunits...

Ngày tải lên: 06/03/2014, 00:21

... pathway and HO-1 regulation in PC12 cells [29,30] This may be through nuclear factor E2-related factor- 2 (Nrf2), a member of the cap’n’collar family of basic leucine transcription factors and a ... different transcription factors that may be involved in mediating the effect of insulin on HO-1 expression, including heat shock transcription factor- 1 (HSF-1), hypoxia-inducible factor- 1 (HIF-1) and ... pathway and its downstream effectors [23] This pathway is a key signal transducer of many growth factors and cytokines and has been implicated in the regulation of cell growth, cell migration and...

Ngày tải lên: 07/03/2014, 12:20

... d-Galinduced aging is still unknown Mitochondrial transcription factor A (TFAM) plays important roles in mtDNA replication and transcription, and the structure ⁄ organization of mitochondrial ... controlled by nuclear-encoded transcription and replication factors that are translocated to the mitochondria Both TFAM and mitochondrial-specific Pol-c are required TFAM is a key factor involved in directly ... dark cycle, and were fed standard rodent chow and water All experimental procedures were performed under the supervision of our Institutional Animal Care and Use Committee DNA extraction After...

Ngày tải lên: 14/03/2014, 23:20

... regulated by Cdx homeodomain proteins and GATA factors [14–16] Thyroid transcription factor- 1 (TTF-1) is an important factor during lung morphogenesis [17–20] and drives the expression of several ... GATA factors in the regulation of Muc5b expression In addition to TTF-1, GATA factors and, especially, GATA-6 are important factors in lung morphogenesis and are known to regulate TTF-1 and synergize ... 10) and cold T84 (lane 16); supershift analysis with anti-GATA-4 (lanes 5, 11 and 17), anti-GATA-5 (lanes 6, 12 and 18) and anti-GATA-6 (lane 7, 13 and 19) IgGs The DNA–protein complex (GATA) and...

Ngày tải lên: 15/03/2014, 00:20

... PHL and ASR PHL and ASR PHL and HXPR PXHXH Transcriptional repressor of neuronal genes in non-neuronal tissues [39,40] Transcriptional repressor and activator [36,41] Transcriptional repressor and ... [49] Transcription factor IIE, a subunit (TFIIE a) [50] casein kinase II-a (CKII-a), ETS-1 and the a subunit of transcription factor IIE (TFIIE-a) (Table 2) REST and YY1 have one HXPR motif and ... the zinc-finger transcription factors YY1 and Sp1 Proc Natl Acad Sci USA 90, 6145–6149 58 Yao YL, Yang WM & Seto E (2001) Regulation of transcription factor YY1 by acetylation and deacetylation...

Ngày tải lên: 16/03/2014, 18:20

... by Sp family transcription factors [19] On the other hand, competitive bandshift demonstrated that the three Sp bands were only slightly reduced by Kmer and Lmer (Fig 6A, lanes and 6), whereas ... on interactions between different transcription factors There is strong evidence demonstrating that transcription factors belonging to the Sp1 family interact with other transcription factors, ... Sp transcription factors In addition, Sp1 and Sp3 transcription factor chromatin immunoprecipitation demonstrated in vivo binding of these proteins to P1 promoter Our results suggest that Sp transcription...

Ngày tải lên: 23/03/2014, 07:20

... the reaction [see bottom of (B) for precise additions] Transcription reactions were processed as described in Fig (A) Transcription factors and RNAPII preparations used in the reconstituted transcription ... basal transcription from TATA-containing promoters and TATA-less promoters PC4 or TFIIEb was added to the transcription reactions, as indicated at the bottom of the figure Transcription reactions ... basal transcription in Sc pombe A B C Fig Stimulation of transcription by PC4 in a reconstituted in vitro transcription assay is dependent on Mediator and TFIIA All in vitro transcription reactions...

Ngày tải lên: 23/03/2014, 07:20

... Cereghini, S (1996) Liver-enriched transcription factors and hepatocyte differentiation FASEB J 10, 267–282 Pontoglio, M (2000) Hepatocyte nuclear factor 1, a transcription factor at the crossroads of ... observed by the overexpression of the transcription factors, Pax8 and lim1, and this effect was additive [44] Furthermore, the artificial expression of Pax8 and lim1 in the Xenopus embryo induced ... the transcription factors, Pax8 and lim1, in Xenopus embryos led to the development of an abnormally large pronephros, and to the formation of ectopic pronephric tissue [44] As both these transcription...

Ngày tải lên: 23/03/2014, 13:20

... in CpG islands containing and TATA-free promoters [10–12] Typically, GC-rich promoter regions lack TATA or DPE core elements, and are frequently found to be bound with transcription factor Sp1 ... [29]; (b) 1120 interaction model – the interaction between Sp1 and AP-2 affects formation of the Sp1–DNA complex [23]; (c) competing model – the AP-2 and Sp1 binding sites overlap, and the AP-2 binding ... Activation of transcription by two factors that bind promoter and enhancer sequences of the human metallothionein gene and SV40 Nature 325, 368–372 Getman DK, Mutero A, Inoue K & Taylor P (1995) Transcription...

Ngày tải lên: 30/03/2014, 02:20

... Immediate-Early ICP4 and ICP22 genes RE-1/NRSE is the binding site of RE1-Silencing Transcription factor/ Neuronal Restrictive Silencer Factor (REST/NRSF) [8] REST/ NRSF is a zinc finger transcription factor ... isolation and RT-PCR pSG28 and control plasmid (1:1) pSG28 and pFLAG-REST (1:1) pSG28 and pCMVp73 (1:1) pH4-2 and control plasmid (1:1) pH4-2 and pFLAG-REST (1:1) pH4-2 and pCMVp73 (1:1) D The RT-PCR ... effect of REST/NRSF on ICP22 promoter pICP22 and control plasmid pICP22 and pFLAG-REST (1:1) pICP22 and pFLAG-REST (1:2) pICP22 and pCMVp73 (1:1) pICP22 and pCMVp73 (1:2) The asterisk P values represent...

Ngày tải lên: 18/06/2014, 18:20

... through a better understanding of the alteration and expression of transcription factors that regulate cellular growth, differentiation and apoptosis Brn-3 transcription factors (Brn-3a, 3b, 3c) ... POU transcription factor and inhibits its ability to activate transcription Cancer Biol Ther 2002, 1:428-32 18 Irshad S, Pedley RB, Anderson J, Latchman DS, Budhram-Mahadeo V: The Brn-3b transcription ... ascites tumor cells and ovarian cancer cell lines consistent with novel distinct role of this factor in the progression and recurrence of this disease Methods and materials Antibodies and reagents Mouse...

Ngày tải lên: 20/06/2014, 07:20

... Arabidopsis and Petunia, MYB transcription factors require a bHLH partner for the trans-activation of flavonoid structural genes [17,21] Recently, two bHLH transcription factors (VvMYC1 and VvMYCA1) and ... with chromatin remodelling factors, general transcription factors (GTFs) of the RNA polII pre-initiation complex, and can also affect initiation of the transcription and elongation [44,45] The ... in a weaker interaction between this protein and yeast general co-activators of the RNA polII complex Indeed, transcription factors act in several ways through protein interactions to enhance...

Ngày tải lên: 11/08/2014, 11:21

... subgroup (AtMYB30, 31, 94 and 96) On the other hand, the grape accession protein ABK59040 was closely related to AtMYB30 and AtMYB31 and to a lesser extent to AtMYB94 and AtMYB96 (Figure 1A) Hereafter, ... ACF21938 and ABK59040 as VvMYB60 and VvMYB30, respectively Based on these results, we further divided Subgroup1 into Subgroup 1.1, (AtMYB30, AtMYB31, AtMYB94 and AtMYB96) and Subgroup 1.2 (AtMYB60 and ... first intron and the third exon, which were longer in the grape genes compared to AtMYB60 Expression of VvMYB30 and VvMYB60 in grape tissues and in response to hormonal and stress factors qPCR...

Ngày tải lên: 11/08/2014, 11:21