6 7 and 8
Cloning genes by PCR
... interest and identify an EST, then this means that a cDNA clone of your target gene is available In most cases 30 000 15 000 500 75 0 87 5 940 470 235 120 60 60 M (B) M 75 000 50 000 37 500 18 75 0 12 ... 3, 5, 7, 8) while other clones show no apparent inserts (4, 6) Photography kindly provided by A Neelam (University of Leeds) 2 36 PCR mRNA AAAAAAAAAA oligo-dT GGG + First-strand synthesis and dC ... Proc Natl Acad Sci USA 85 : 89 98 9002 Chenchik A, Diachenko L, Moqadam F, Tarabykin V, Lukyanov S, Siebert PD (19 96) Full-length cDNA cloning and determination of mRNA 5′- and 3′-ends by amplification...
Ngày tải lên: 25/10/2013, 22:20
Báo cáo khoa học: The lactate dehydrogenases encoded by the ldh and ldhB genes in Lactococcus lactis exhibit distinct regulation and catalytic properties ) comparative modeling to probe the molecular basis pdf
... Fru(1 ,6) P2 No KPi 50 mM KPi 100 mM KPi No Fru(1 ,6) P2 No KPi mM KPi 50 mM KPi 100 mM KPi a c LDH pH 6. 0 pH 7. 0 pH 6. 0 pH 7. 0 100 ± 6. 2 110 ± 2 .6 – 100 ± 2 .6 88 ± 0 .7 – 100 ± 0.2 100 ± 0 .7 90 ± 0 .6 ... LDH pH 6. 0 a pH 7. 0 d Km NADH (lM) Km pyruvateb (mM) Kact Fru(1 ,6) P c (lM) 77 ± 1.3 ± 0.1 0.2 ± 0.03 pH 6. 0 d 364 ± 2.9 ± 0.3 140 ± 16 pH 7. 0 54 ± 1.5 ± 0.2 0.3 ± 0. 06 58 ± 1 .7 ± 0.2 0.5 ± 0.05 ... B 10 30 50 70 90 60 Pyridine nucleotides (mM) Concentration (mM) A 160 40 20 -10 10 30 50 70 90 C 60 Concentration (mM) 40 6. 8 30 20 6. 4 10 -10 Intracellular pH 7. 2 50 6. 0 10 30 50 70 90 Time...
Ngày tải lên: 16/03/2014, 05:20
Genes and Autoimmunity - Intracellular Signaling and Microbiome Contribution by Spaska Angelova Stanilova pot
... (%) 56 74 130 101 27 1 86 1 28 SLE (n=1 57) ( 36% ) ( 47% ) (73 %) (74 %) ( 27% ) (59%) (41%) 59 83 142 1 07 24 201 131 ( 36% ) (50%) ( 86 % ) (64 %) (14%) (61 %) (39%) 0 .84 4 0 .79 3 0 .81 4 (0.41±1 .72 ) (0.40±1. 56) (0.43±1.54) ... (n=53) 8. 88 ± 3 .79 male (n =7) 8. 57 ± 2 .72 female (n= 46) 8. 93 ± 3.95 male vs female p = 0 .82 Healthy controls total (n =66 ) 32.99 ± 24 .84 male (n=13) 60 . 98 ± 31.32 female (n=53) 26. 12 ± 17. 34 Significance ... 0 .61 4 0. 471 - χ2 =0.203 HC (n= 166 ) OR (95% CI) p OR 0.939 (95% CI) ref (0. 56 1. 57) p 0 .79 9 p=0.934 0.9 47 - ref (0 . 68 ±1.31) ref 0.5 - 0 .73 5 - 0.994 1. 185 1.0 56 (0 .61 ±1 .61 ) (0. 58 2.42) ref (0. 76 1. 47) ...
Ngày tải lên: 17/03/2014, 21:20
Báo cáo khoa học: "Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD" docx
... C5 C6 P1 P2 P3 P4 P5 P6 P7 4 388 8 4 388 9 4 389 2 4 389 4 29 (4-FS) 40 (13 98) 41 ( 973 ) 42 (75 ) 43 (7 96) 44 (1 489 ) 3009 -88 (3D) 3 077 -88 (3E) 3104 -88 (3C) 3299 -85 (3A) C7 -88 (4E) C 681 - 87 (4D) C999- 87 (4B) ... Immunomagnetic separation E coli O1 57: H7 Rapid kit 99. 58 (1 , 67 5/1 , 68 2)b 99.41 (1 , 67 2/1 , 68 2) 99.35 (1 , 67 1/1 , 68 2) 0.42 (7/ 1 , 68 2) 0.59 (10/1 , 68 2) 0 .65 (11/1 , 68 2) a No of positive/No of samples examined ... isolates and 17 U.S strains Lane M, kb DNA marker; lane 1, P01 07 26- 18; lane 2, P01 07 26- 21; lane 3, P01 07 26- 22; lane 4, P01 07 26- 23; lane 5, P01 07 26- 24; lane 6, P01 07 26- 25; lane 7, P01 07 26- 26; lane 8, ...
Ngày tải lên: 07/08/2014, 18:21
Báo cáo khoa học: " Identification and prevalence of Ehrlichia chaffeensis infection in Haemaphysalis longicornis ticks from Korea by PCR, sequencing and phylogenetic analysis based on 16S rRNA gene" docx
... 96. 4 96. 4 96. 4 96. 2 96. 2 96. 2 95 .6 94.1 92.3 92.1 91.1 0 98. 5 97. 9 97. 7 97. 4 97. 2 96. 9 96. 4 96. 4 96. 4 96. 2 96. 2 96. 2 95 .6 94.1 92.3 92.1 91.1 6 99.5 99.2 96. 7 96. 4 96. 2 96. 4 96. 4 96. 4 96. 7 96. 1 ... 97. 4 97. 2 96. 9 96. 4 96. 4 96. 4 96. 2 96. 2 96. 2 95 .6 94.1 92.3 92.1 91.1 100 100 98. 5 97. 9 97. 7 97. 4 97. 2 96. 9 96. 4 96. 4 96. 4 96. 2 96. 2 96. 2 95 .6 94.1 92.3 92.1 91.1 100 98. 5 97. 9 97. 7 97. 4 97. 2 96. 9 ... 96. 4 96. 7 96. 1 96. 7 95.9 93 .6 93.1 93.1 92.0 8 98. 2 96. 2 96. 4 95.9 95 .6 95 .6 95 .6 96. 4 95.4 96. 7 95.1 93.1 92.5 92 .6 91 .8 9 96. 9 96. 7 96. 4 96. 1 96. 1 96. 6 95.9 95.9 95.9 96. 1 94 .6 92 .8 93.1 92.3 10...
Ngày tải lên: 07/08/2014, 18:21
Báo cáo y học: "Analysis of bacterial DNA in synovial tissue of Tunisian patients with reactive and undifferentiated arthritis by broad-range PCR, cloning and sequencing" pps
... UA) AY0 083 15 1,420 98 .73 Aeromonas sp (1 UA) U8 86 5 6 1,3 96 99. 36 Aeromonas sp (2 UA) AF0990 27 1,3 36 98. 58 Afipia genosp (1 UA) U 87 7 73 1,3 36 97. 38 Afipia genosp (1 ReA) U 87 7 79 1,335 99 .62 Afipia ... 47 38 +++ 96 36 +++ 84 50 +++ 104 48 +++ 34 26 ++++ 24 24 ++ 105 48 ++ 114 41 ++ 72 42 10 ++ 75 25 11 ++ 96 46 12 ++ 74 34 13 ++ 1 18 42 14 ++ 60 28 15 ++ 101 49 16 ++ 48 40 17 + 96 35 18 + 48 ... ReA) U 87 7 75 1,2 56 97. 00 Afipia genosp (1 ReA) U 87 7 77 1,3 37 99.55 Corynebacterium coyleae (1 UA) X 964 97 1, 360 99.04 Escherichia coli (1 ReA) AP0090 48 1, 389 99 .71 Escherichia sp (2 ReA) DQ3 375 03...
Ngày tải lên: 09/08/2014, 10:23
Báo cáo y học: "Tetra-O-methyl nordihydroguaiaretic acid (Terameprocol) inhibits the NF-B-dependent transcription of TNF-a and MCP-1/CCL2 genes by preventing RelA from binding its cognate sites on DNA" ppsx
... and TLR -8 are double and single stranded RNA, respectively In these experiments we used the artificial ligands poly(I:C) for TLR-3 and resiquimod (R -84 8) for TLR -8 HEK293/TLR3 and HEK293/TLR8 ... Main Campus Dr., Suite 100, Raleigh, NC, 2 76 0 6) and Grant # R56AI 070 84 8-01A1 from the National Institutes of Health The authors wish to thank D Eads and K Belanger-Crook for their technical assistance ... Egr-1, c-Jun, and NF-kappaB transcription factors J Biol Chem 19 97, 272 : 177 95- 1 78 01 Oyegunwa et al Journal of Inflammation 2010, 7: 59 http://www.journal-inflammation.com/content /7/ 1/59 23 DuBois...
Ngày tải lên: 11/08/2014, 03:20
Báo cáo khoa học: " Detection and identification by PCR of Clostridium chauvoei in clinical isolates, bovine faeces and substrates from biogas plant" potx
... AN 365 6/05 AN 165 / 06 AN 11 06/ 06 AN 171 7/ 06 AN 181 8/ 06 AN 2091/ 06 B 164 76 / 07 B 175 20/ 07 B 19034/ 07 P 2500/ 07 P 285 5/ 07 P 482 2/ 07 P 24 46/ 08 B 29245/ 08 B 322 86 / 08 P 30 86 / 08 P 3240/ 08 B 41901/ 08 B ... AN 165 / 06 AN 11 06/ 06 AN 171 7/ 06 AN 2091/ 06 B 164 76 / 07 B 175 20/ 07 B 19034/ 07 P 285 5/ 07 P 482 2/ 07 P 24 46/ 08 B 29245/ 08 B 322 86 / 08 P 30 86 / 08 P 3240/ 08 B 41901/ 08 B 41902/ 08 B 43014/ 08 B 43531/ 08 P ... plants Bioresource Technology 20 08, 99 : 78 59 -7 86 5 Larsen HE: Risks of bacterial infections when using animal manure and biowaste Dansk Vet Tidskr 1995, 78 : 76 3 - 76 6 Larsen HE, Munch B, Schlundt J:...
Ngày tải lên: 12/08/2014, 18:22
Báo cáo y học: "Wound healing and inflammation genes revealed by array analysis of ''''macrophageless'''' PU.1 null mice" ppt
... Kcnk7 Adcy4 EST AW0 461 24 Myf6 Osp94 Plaur Fv1 EST AI5 364 57 Ctla2a EST AA0 083 87 Pex5 Map3k1 EST AA 8 67 77 8 Ptgls Pld1 EST AW0 461 50 Car2 Kic1 Ppt2 EST AA 960 6 57 Hck EST AI8521 48 Gaigt1 Igh -8 Mal IL8rb ... Stal1 EST AW12 372 9 EST AI 882 555 Vdr EST AA 881 294 EST AI845009 EST AW123223 Rock2 EST AA25 9 68 3 EST AI83 961 1 EST AI849305 Klkbp Ereg Sdcbp1 Mmp11 Alrp Tac1 EST AA2 76 9 48 Eps8 EST AA73 87 76 Nat2 Pfkp ... Cyp51 Zfp292 Sdfr2 EST AA 982 595 Zt3 EST C801 08 Sprr1b EST AA690 483 Atp7a Mtap6 Amd2 EST AW04 76 1 6 Mbd4 EST AI6 065 77 EST AA612212 EST C812 57 Narg1 Diap3 Wnt5a EST AI 561 5 67 Stag1 Glf Egfr Ube3a Grpei2...
Ngày tải lên: 14/08/2014, 14:21
Generation of mouse graves ophthalmopathy model with full length TSH receptor plasmid and cytokine evaluation by real time PCR
... Swiss Outbred 57 Summary of Findings 62 4.1 Changes after Genetic Immunization 62 4.2 Th1 and Th2 Cytokine Expression 62 V DISCUSSION 66 Technical Difficulties 66 Discussion of Results 68 2.1 Genetic ... GO 74 2.4 Clinical relevance of differences in cytokine profile in Mouse model 75 2.5 Limitation of current study 75 2 .6 Possible Future Work / Experiments 76 VI CONCLUSION 77 VII REFERENCES 78 ... (PCR) 36 Statistical Analyses 37 IV RESULTS 38 RNA Extraction 38 Immunization of Balb/c and Swiss Outbred Mice 43 2.1 Weight 43 2.2 Total T4 46 2.3 FACS for TRAB Detection 47 2.4 TBII Assay 48 2.5...
Ngày tải lên: 07/10/2015, 09:55
ESTROGENIC ACTIVITY LEVEL OF NAKDONG RIVER BASIN AND ITS CONTROL BY WATER TREATMENT PROCESSES
... 1.43±0.09 18. 56 5.4 7 . 67 ±0. 28 0.50±0.03 1 .83 ±0.01 2.53±0 .72 8. 22±0 .62 0. 47 0.02 1. 86 0.04 1 .63 ±0.04 8. 53±0. 17 1.40±0. 07 4.22±0.04 3. 17 0.23 8. 20±0.23 0 . 67 ±0.03 3 .69 ±0.01 9.13±1.14 7. 17 0.12 0.45±0.02 ... 1 .79 ±0.04 1. 17 0. 08 1.04±0. 07 2 .74 ±0.04 1.24±0.09 1. 58 0.11 3. 27 0.02 1.24±0. 08 46. 73 ±4.21 1 .82 ±0.13 3.94±0. 16 1. 38 0.04 51.34±3.59 1 .75 ±0.12 4. 36 0. 17 1 . 68 ±0.01 2.40±0. 17 1.19±0. 08 3 .83 ±0. 06 ... 4 .60 ±0. 18 4. 16 0.12 1 .69 ±0.19 2.95±0.12 2.93±0 .83 4. 18 0. 18 5. 47 0. 58 3.94±0.39 7. 93±0. 08 3.92±0. 06 1 .83 ±0.09 4. 97 1.20 3.02±0.13 1. 46 0.09 2 .80 ±0. 17 4.14±0.41 3.92±0.11 1 .83 ±0. 08 3. 08 0.23 3. 87 0.32...
Ngày tải lên: 05/09/2013, 08:40
Moment and Shear, Generated by DL on the Composite Section
... 3 86 . 19 334.00 260 .94 1 87 . 88 93.94 0.00 0.00 3 57. 89 71 5 . 78 9 87 . 70 1259 .63 14 56. 97 165 4.31 175 1.31 184 8.31 18 76 . 07 1903 .83 18 76 . 07 184 8.31 175 1.31 165 4.31 14 56. 97 1259 .63 9 87 . 70 71 5 . 78 3 57. 89 0.00 0.00 ... 3 .88 2 20 .84 8 11 1.55 3 .88 2 20 .84 8 12 1 .6 3 .88 2 20 .84 8 13 1 .65 8 .73 7 15.993 14 1 .7 8 .73 7 15.993 15 1 .75 8 .73 7 15.993 16 1 .8 8 .73 7 15.993 17 1 .85 9. 87 5 14 .85 5 18 1.9 9. 87 5 14 .85 5 19 1.95 9. 87 5 ... 513 .75 65 7 .60 76 0 .35 86 3 .10 924 .75 9 86 . 40 10 06. 95 10 27. 50 10 06. 95 9 86 . 40 924 .75 86 3 .10 76 0 .35 65 7 .60 513 .75 369 .90 184 .95 0.00 4 87 . 80 67 9.35 87 0 .90 1010.09 1149.29 1230.59 1311 .89 13 38. 07 1 364 .24...
Ngày tải lên: 29/09/2013, 12:20
a discourse analysis of opening and closing speeches by masters of ceremonyon reality television showsin american english versus vietnames
... in AOSs and VOSs Types of phrases American Vietnamese Occurrence Rate Occurrence Rate Noun phrases 65 3 47. 15% 10 26 57. 22% Verb phrases 3 68 26. 57% 584 32. 58% Adjective phrases 364 26. 28% 183 10.20% ... VOSs 66 Table 4.10 Reiteration of Lexican Items in AOSs and VOSs 67 Table 4.11 Grammatical Cohesion in AOSs and VOSs 70 Table 4.12 Reference in AOSs and VOSs 71 Table 4.13 Conjunctions in AOSs and ... 63 4.4.2 Grammatical Cohesion 69 4.5 THE GENERAL OUTLINE OF ACSS AND VCSS 87 4.5.1 Transition 87 4.5.2 The Body 89 4.5.3 Departing 91 4 .6 THE...
Ngày tải lên: 26/11/2013, 12:41
A RESEARCH ON ENGLISH INVERSION AND DIFFICULTIES ENCOUNTERED BY ENGLISH MAJORS OF HAI PHONG PRIVATE UNIVERSITY
... (THM, 1 985 :110) [ 16] Has he (got) to go? (THM, 1 985 :110) [ 17] Dare you it? (ALG, 1 988 :2 36) [ 18] Need you leave so soon? (ALG, 1 988 :229) According to Quirk.R (1 973 ), apart from the questions and the ... between the speaker and the listener [ 96] What is your brother like? Anh trai bạn nhìn giống thế? (ALG, 1 988 : 265 ) [ 97] How much does this cost? Cái giá nhỉ? (ALG, 1 988 : 2 68 ) [ 98] Whose umbrella ... following examples: [ 57] Đằng trước công nhân (BDQ, 19 98: 154) [ 58] Trong nhà có khách (BDQ, 19 98: 1 56) [59] Trên bàn có lọ hoa (BDQ, 19 98: 1 58) [60 ] Dưới gầm bàn trại muỗi (BDQ, 19 98: 1 38) According to...
Ngày tải lên: 11/12/2013, 23:48
A study on imperative and errors made by vietnamese learners
... 4/50 8% 36/ 50 72 % 47/ 50 94% 10 3/50 6% 11 11/50 22% 12 16/ 50 32% 13 23/50 46% 14 17/ 50 34% 15 12/50 24% 16 8/ 50 16% 17 17/ 50 34% 36 18 13/50 26% 19 21/50 42% 20 38/ 50 76 % 21 43/50 86 % Through ... University And the result of the survey is given in the table as follow: Percentage of incorrect Number No of incorrect answers answers 38/ 50 36% 12/50 24% 45/50 90% 6/ 50 12% 43/50 86 % 39/50 78 % 4/50 8% ... command 24 4.3 Giving an advice 26 4.4 Giving a request 26 4.5 Giving a warning and an instruction 27 4 .6 Giving a prohibition 28 4 .7 Giving...
Ngày tải lên: 11/12/2013, 23:51
A study on subjuntive and errors made by vietnamese learners
... http://tailieu.vn/xem-tai-lieu/subjunctive-mood.393 37. html E.g - I would rather go to a movie tonight than study grammar [Azar, Understanding and using English Grammar, (1 989 : 366 )] - My grandmother would rather live ... quickly.[(Quirk, (1 973 :50)] - If only he did not smoke! [Thomson and Martinet, (19 86 :204)] - It is neccessary that you learn by heart this lesson now - God save the Queen! [Quirk, (1 973 :50)] 18 Chapter ... that… - If need be… [Quirk, (1 973 :50)] (http://www.tranphu.com/51/viewtopic.php?f=2 18& t=2 2 68 8) A wish can also be expressed by “may + infinitive” (in the present) and “might + infinitive” (in the...
Ngày tải lên: 11/12/2013, 23:53
Tài liệu How to Win Friends and Influence People by Dale Carnegie docx
... bacon and soap and lard for Armour and Company His territory was up among the Badlands and the cow and Indian country of western South Dakota He covered his territory by freight train and stage ... butter too thick on your bread And as you started off to play and I made for my train, you turned and waved a hand and called, "Goodbye, Daddy!" and I frowned, and said in reply, "Hold your shoulders ... book and in his work Thousands of people all over the world are being trained in Carnegie courses in increasing numbers each year And other thousands are reading and studying How to Win Friends and...
Ngày tải lên: 24/01/2014, 00:20