5nanofiber matrices for differentiation of stem cells

... behavior of stem cells in vivo Overall, microfluidics tools can be used for spatio­ temporal control over the stem- cell microenvironment, so that the ideal ex vivo niche for cell survival and differentiation ... configuration of microchips is a major advantage of microfluidics-based cell-culture systems, and the ease with which fluid flows can be controlled over time and space Human embryonic stem cells (hESCs) ... automation of hESC growth and differentiation in vitro on microfluidics platforms produces more standardized outcomes Many investigators believe that the stress of manual handling of hESCs is...

Ngày tải lên: 06/08/2014, 19:21

... ancestral for all bilaterian animals Because of their unusual neoblast system, platyhelminths and acoels are particularly attractive invertebrate models for stem- cell research They present a number of ... properties of neoblasts and their non-neoblast progeny is unquestionably an important component of understanding the functioning of these remarkable stem cells However, current research in stem- cell ... germ line); these stem cells exhibit high rates of turnover, undergoing continual self-renewal and production of differentiated progeny; and neoblasts are the only proliferative cells in the body...

Ngày tải lên: 06/08/2014, 19:21

... common hepatopancreatic stem cell, or transdifferentiation of adult cells Tissue-specific adult stem cells are not pluripotent, but recent evidence has suggested that rare stem cells with high developmental ... transformation of normal stem cells Work in Weissman’s laboratory has established the role of a leukemia stem cell in leukemia If the results hold true for other tumors, the new challenge of cancer ... between the host and grafted cells Further analysis is required if we are to identify any other, more appropriate, sources of stem cells Development of a cell-based therapy for liver failure and inherited...

Ngày tải lên: 14/08/2014, 14:21

... milk-secreting -cells (Buehring 1972) 1.4 Adult Stem Cells Stem cells are defined by their functional attributes Typically, one would define the stem cells of a particular tissue as undifferentiated cells ... 131 5.6.2.1 5.6.2.2 Isolation of Cells 131 Culture of SP Cells 132 Isolation of Stem/ Progenitor Cells in Expressed HBM by CD133 133 vii Table of Contents 6.1 Introduction ... Location of the Putative Stem Cells 33 1.4.1.3 Characterisation of Mammary Epithelial Stem Sells 34 1.4.1.4 Association with Tumorigenesis 38 1.4.2 Haemopoietic Stem and Progenitor Cells...

Ngày tải lên: 11/09/2015, 09:57

... 2.2 Stem cells in tissue regeneration 2.2.1 Embryonic stem cells 2.2.2 Adult stem cells 2.2.3 Adipose derived stem cells Chapter Chapter v 2.2.4 Hepatic differentiation of adipose derived stem ... Injectable deliverey systems for stem cell therapy 20 2.4.1 Effect of mechanical cues on stem cells 21 2.4.2 Effect of biomolecular cues on stem cells 22 2.4.3 Hydrogels for stem cell therapy 23 ... of stemness marker genes on gelatin 49 microspheres 4.2.4 Adipogenic and osteogenic differentiation of 49 adipose derived stem cells 4.2.5 Hepatic differentiation of adipose derived stem 51 cells...

Ngày tải lên: 09/09/2015, 11:18

... the three Drosophila isoforms and two Leptinotarsa isoforms Such quantitative differences may prove important for in vivo functions In Manduca, the presence of a B-isoform increases transcriptional ... quantitative level of transcriptional activity, with both its own EcR isoforms, and with the two L decemlineata EcR isoforms Discussion 10 B 40 3.0 A controlled assessment and comparison of the Leptinotarsa ... quantitative level of transcription for a given isoform, it had no effect on relative ligand responsiveness Importantly, the relative induction by individual agonists was species-specific for all of the tested...

Ngày tải lên: 18/02/2014, 08:20

... types of cells It is believed that stem cells also form reservoirs of repair cells to replace cells and tissues that degenerate over the life span of the organism The dual capacity of stem cells for ... human cells was significant because of its implications for improved health The dual capacity of hES cells for self-renewal and for differentiation into repair cells offers great potential for ... characterization of stem cells for purposes of standardization and for validation of results The safe handling and storage of blastocysts and stem cell material and the conditions for transfer of such...

Ngày tải lên: 06/03/2014, 15:20

... Conditional disruption of Ptb in ES cells Ptb) ⁄ flox ES cells were generated by expression of Cre in Ptb) ⁄ flox-hyg ES cells Ptb) ⁄ ) ES cells were generated by infection of Ptb) ⁄ flox ES cells with a ... number of Trypan blue-staining-negative cells to that of total cells Cells (3 · 105) were seeded on growth medium and counted every day for days of culture The circles indicate the values for wild-type ... blue solution The value of relative viable cells was calculated as the ratio of the number of Trypan blue-negative cells to that of total cells The apoptosis assay was performed using an ApopTag...

Ngày tải lên: 07/03/2014, 00:20

... of foot faults in the left and right forelimbs The sum of foot faults (SOFFF) in the forelimbs is defined as the number of faults of right and left forelimbs, whereas the differentiation of forelimb ... foot faults (DOFFF) is the difference between the number of faults for the right and left forelimbs SOFFF and DOFFF were used as an index of motor deficit CSF aspiration 80 μL of CSF were aspirated ... media of culture passages five to seven of primary neural stem cells from cortices of E16 Sprague-Dawley rat embryos in three separate experiments A preponderance of NGF in pg per day of culture of...

Ngày tải lên: 18/06/2014, 16:20

... rpOka∆58-infected cells were harvested at 24 hours p.i Cells were extracted with mL of TRIzol Reagent (Invitrogen) and 200 µL of chloroform Cell extract was centrifuge and supernatant was added with 500 µL of ... µL of DEPCtreated water Seven µL of solution was added with µL of 10× DNase buffer and µL of DNaseI and incubated for 20 minutes followed by added with µL of 25 mM EDTA and incubated at 60°C for ... were assessed for the property of cellto-cell spread by comparison of plaque sizes Briefly, MRC-5 cells or MeWo cells were infected with approximately 50 PFU of cell-free viruses of rpOka or rpOka∆58,...

Ngày tải lên: 20/06/2014, 01:20

... the well of a 96-well plate (34 mm ) for 24 h For every cavity, the total number of cells and the number of fluorescent cells were determined by microscopy, and the ratio of fluorescent cells was ... the suspension cells started to adhere and form neuronal networks (Figure 3c) Mesenchymal stem cells (MSCs) were used as a model for a sensitive in vitro system [43] The morphology of the human ... effect of miniaturization on the differentiation capacity of hMSCs Human MSCs were cultured for 18 days in adipogenic differentiation medium Lipid droplets, which were formed as a result of adipocytes,...

Ngày tải lên: 21/06/2014, 00:20

... alcohol to burn off 19 Do not let the sterilized portion of the instrument contact any nonsterile material before use Let the heated part of the instrument cool for ∼10 sec before use 20 After ... spill for 30 Drain the contents of the catch basin into a container suitable for final sterilization by autoclaving Visually inspect the working interior of the laminar cabinet for evidence of dried ... procedure for localizing the source of any remaining contamination This can be achieved with a series of opened plates of trypticase soy agar and Emmons’ modification of Sabouraud’s agar systematically...

Ngày tải lên: 11/07/2014, 21:20

... marrow stem cell transplantation for severe systemic lupus erythematosus of long duration Lupus 1997, 6:545-548 Burt RK, Traynor A, Ramsey-Goldman R: Hematopoietic stemcell transplantation for systemic ... cyclophosphamide dose of 255 mg/kg, compared with 200 mg/kg in the study of Brodsky and colleagues By having to mobilize stem cells, the transplant protocol allows for infusion of a significantly ... compared with the same dose of cyclophosphamide without hematopoietic stem cell support, and also to hasten stem cell regeneration of the immune system under the umbrella of the ATG effect Based...

Ngày tải lên: 09/08/2014, 01:23

... rationale for that procedure is a rapid eradication of autoreactive effector cells while sparing stem cells because their high content of aldehyde dehydrogenase Page of 10 (page number not for citation ... absence of GVHD, an increase of the anti-inflammatory IL-10 is described which may promote the formation of T-regulatory cells Apart from the increase of IL-10 serum levels, reduced levels of interferon, ... administration of mesenchymal stem cells or T-regulatory cells [47] In a recent prospective clinical trial, 56 patients with severe acute GVHD were treated with mesenchymal stem cells obtained...

Ngày tải lên: 09/08/2014, 10:23

... dosage imbalance of HSA21 genes Results Validation of an inducible/exchangeable system for generation of transgenic mES cells In order to generate a library of mES transgenic lines of selected HSA21 ... to Additional file for more general information about these genes) For a subset of the selected genes, there is evidence for the presence of different alternatively spliced isoforms that may differ ... Page of 18 onic stem (mES) cell clones capable of the inducible overexpression of each one of 32 selected genes, 29 murine orthologs of HSA21 genes and HSA21 coding sequences, under the control of...

Ngày tải lên: 09/08/2014, 20:22

... sequences of their viral isolates for evidence of drug specific resistance mutations Genotyping revealed that 5/9 (55.5%) had drug specific mutations Of these patients, had drug resistance for all ... viruses As entry inhibitors for CCR5 and CXCR4 co-receptors are currently being used for HIV treatment, fast and reliable methods for determination of viral tropism will be of value to clinicians ... osteosarcoma cells) expressing CCR5, CXCR4 with CD4 or CD4 alone (received from NIH AIDS Reagent Program) were used to determine co-receptor use Briefly, cells were seeded at a concentration of 10e5 cells/ well...

Ngày tải lên: 10/08/2014, 05:21

... for conditioning; Phenytoin for prophylaxis of busulfan’s side affect Tacrolimus; methotrexate and mycophenolate mofetil (0.5 g twice a day on days to +28) for GVHD prophylaxis; Caspofungin for ... provision of clinical care of the patient and the collection of data and the review of the manuscript GPY: supplied the acquisition of data, analysis and interpretation of data, drafting of manuscript; ... Medical University; Member of the Asian-Pacific Society of Hematology; Member of Blood Branch of the Chinese Medical Association; Member of the Chinese hematopoietic stem cell transplant group;...

Ngày tải lên: 10/08/2014, 21:23

... CSP/MMF/ MTX short 1.8 220 Not done 55% 47% 2-year 19% for SR 31% for HR 12% for SR 39% for HR 2-year 68% for SR 42% for HR Japan, 2006[39] Journal of Hematology & Oncology 2008, 1:27 JoneHopkins University ... blood with bone marrow stem cells, both depleted of T cells, following TBI-based conditioning regimens[16-18] In their early report, the median numbers of CD34+ and CD3+ cells were 10.8 × 106/kg ... occurrence of GVHD, subsequent follow-up revealed problems of both late rejection and/or graft failure, which led to high TRM[16] The availability of techniques for positive selection of CD34 cells...

Ngày tải lên: 10/08/2014, 22:20

... weight of AGMA1-75 is reduced of 25% and that one of ISA23-75 of only 12% Atomic Force Microscopy AFM images reported in Figure show the morphology of different substrates TCPS surface is uniform ... substantial amount of cells on AGMA1-75 and TCPS show an polygonal-like morphology typical of the phenotype of adhered MDCK cells, whereas on ISA23-75 the number of adhered cells is still very ... Synthesis of hydrogels Synthesis of hydrogels Scheme of the procedure for the synthesis of hydrogels Step A: functional oligomer preparation; step B: in situ crosslinking Page of 15 (page number not for...

Ngày tải lên: 11/08/2014, 00:22