5 1 analysis and bcg internalization assay

... Differential Analysis (RDA)…………………… 40 2.2.3 5 1 analysis and BCG internalization assay ………………………………… 41 2.2.3 .1 Integrin 5 1 receptor analysis ………………………………………………… 41 2.2.3.2 BCG internalization assay ……………………………………………………… ... of lyo BCG treatment…………………………………………………………………………… 11 0 4.4 Discussion 2…………………………………………………………………… 11 1 4.4 .1 Integrin 5 role in BCG internalization, cytotoxicity and ROS production……… 11 1 4.4.2 ... lyo BCG treatment 11 1 Figure 5. 1 Isolation of MptpA 12 2 Figure 5. 2 Phosphatase activity of MptpA in vitro 12 4 Figure 5. 3 Effects of MptpA on cell cycle progression of MGH cell line 1 25 Figure 5. 4...

Ngày tải lên: 11/09/2015, 10:06

... is 3. 61 at 20° [JCS 24 95 19 31; JACS 77 6698 1 955 1 2-Acetylbenzoic acid [57 7 -56 - 01 M 16 4.2, m 1 15 - 11 6O, 11 6 -11 8O Recrystallises from C6H6 and H (15 g /10 0ml) It has pKa in H20 of 4 .10 at O , and the ... m 16 3 -1 65. 5O; JACS 79 452 4 1 957 ; H C A 31 114 2 19 48) [HCA 40 55 4 1 957 1 2-Acetylthiophene (methyl 2-thienyl ketone) [88- 15 - 31 M 12 6.2, m 9.2 -10 .52 "' 10 .45O, - l o , b 77O/4mm, 89- 91 /9mm, 94 .5- 96 .5 /13 mm, ... has m 14 9O (15 lo), and the less soluble has m 17 2 -1 75 (19 10) 4-Nitrophenylhydrazone has m 16 6 -16 7O and the 2,4-dinitrophenylhydrazonehas m 11 4 -11 5O [HCA 17 12 9, 14 0 19 34; A 56 4 10 9 19 4 91 N-Acetyl-6N’-methylglycinamide[7606-79-31M...

Ngày tải lên: 05/03/2014, 21:20

... 1 35( 71) , 12 1(24), 10 7 (10 0), 1 05( 20), 91( 33), 79(20), 67 (52 ), 53 (22), 41( 28) 216 (M1 ,13 ), 14 8(30), 13 3 (10 ), 10 9 (10 ), 10 8 (10 0), 1 05( 12 ), 93 (11 ), 91( 16), 79 (17 ), 77( 15 ) 15 4 (M1,36), 11 1(47), 10 8 (50 ), ... 16 7( 75) , 10 9(80), 95( 34), 82(36), 69 (10 0), 68(64), 67( 61) , 55 (73), 41( 67) 236(M1,26), 1 35( 28), 12 1 (10 0), 11 9(29), 10 7( 41) , 93(40), 91( 29), 69(33), 55 (44), 43(28), 41( 46) 218 (M1,8), 13 6(49), 1 35( 71) , ... K4 K5 K6 0.39 0.60 0.77 1. 49 1. 66 3.27 0.33 0 .56 0.76 1. 74 1. 94 4 .53 0 .17 0.32 0 .50 1. 02 1. 15 2.22 0.28 0 .54 0.72 2.08 2 .17 6.49 0.34 0.63 0. 75 1. 90 1. 94 5. 22 0.43 0.72 0.80 1. 49 1 .56 2.99 0 .58 ...

Ngày tải lên: 07/03/2014, 21:20

... distillation 12 12 13 13 14 RECRYST ALLIS ATION Techniques 14 14 Filtration 15 Solvents 15 Choice of solvents Water Petroleum ethers Mixed solvents Recrystallisation Zone refining 15 16 16 16 from ... (FC and HPFC) Paired- ion Chromatography (PIC) Ion-exchange Chromatography Ion-exchange Resins Ion-exchange celluloses and sephadex chromatography) 18 18 18 19 19 20 20 20 20 21 21 21 21 21 21 ... MACROMOLECULES NITRILES 69 70 70 70 70 70 71 71 71 71 71 71 72 72 72 72 73 73 74 74 75 75 75 75 75 NITRO COMPOUNDS NUCLEIC ACIDS PHENOLS POLYPEPTIDES AND PROTEINS QUINONES SALTS With metal ions...

Ngày tải lên: 14/03/2014, 20:20

... nanotubes [11 2] [11 3] [11 4] [1 15 ] [11 6] [11 7] [11 8] [11 9] [12 0] [12 1] [12 2] [12 3] [12 4] [1 25] [12 6] [12 7] [12 8] [12 9] [13 0] by size-exclusion chromatography Anal Chem 20 05; 77 (19 ):62 25 8 Doorn ... bio-nanomaterials [ 15 ] , energy storage media [16 ,17 ] and nanoelectronic devices [18 ,19 ] 1. 1 2 014 2 014 20 15 2 016 2 016 2 016 2 017 2 018 2 018 2 018 2020 2020 20 21 20 21 20 21 20 21 20 21 benzene, ethanol, ... [93] [94] [ 95] [96] [97] [98] [99] [10 0] [10 1] [10 2] [10 3] [10 4] [1 05] [10 6] [10 7] [10 8] [10 9] [11 0] [11 1] CARBON ( 0 ) 0 –2 via organic functionalization J Am Chem Soc 2002 ;12 4(48) :14 318 –9 Banerjee...

Ngày tải lên: 20/03/2014, 13:06

... 636 2 45 74 61 2 75 65 31 15 10 12 9 14 5. 4 2 .1 2.9 1. 2 660 13 1. 3 0.26 927 24 3.0 0.3 0 .13 18 11 71 3.7 0.42 0.36 0.063 0.96 19 59 239 0.30 2.7 10 51 79 0.0 71 0 .19 1. 4 5. 0 8.0 19 20 61 248 35 17 1 266 ... ± 0.90 ± 280 ± 240 ± 11 0 ± 11 0 ± 11 ± 13 ± 64 ± 70 ± ND >50 17 ± 18 ± 23 ± 24 ± GGT AT2 GGT GPT PSOT AT3 AGT 0. 05 0.3 0.04 0.2 0.8 0 .1 1.8 0.07 0.3 0 .1 0.08 10 10 10 10 1 a The estimate of the ... (YP_ 010 112 ), Halorhodospira halophila (YP_0 010 017 22), human (NP_872603), Hydrogenivirga sp 12 8 -5- R1 -1 (ZP_0 217 6974), Hydrogenobaculum sp Y04AAS1 (YP_00 212 1232), Nitrococcus mobilis (ZP_ 011 27 658 ),...

Ngày tải lên: 29/03/2014, 09:20

... 77 81 94 10 8 11 6 12 3 12 8 13 6 14 2 14 4 15 5 16 2 16 8 17 4 18 2 18 9 2 01 211 2 25 2 35 244 2 51 257 259 2 61 262 56 67 78 85 90 94 10 8 12 2 13 1 13 7 14 3 15 1 15 7 16 0 17 1 17 9 1 85 19 0 19 9 206 219 229 243 254 263 ... 13 3 13 7 14 0 14 0 14 2 - 15 -7 11 15 25 36 43 48 53 59 64 66 74 81 86 86 97 10 2 11 2 12 0 13 1 14 0 14 7 15 2 15 7 16 0 16 0 16 2 -4 15 20 24 28 39 51 58 63 68 74 79 82 90 97 10 2 10 7 11 4 11 9 13 0 13 8 15 0 15 9 ... 279 2 81 282 68 79 91 98 10 3 10 8 12 1 13 6 14 6 15 2 15 8 16 6 17 3 17 6 18 7 1 95 202 207 216 223 237 247 262 273 282 290 296 299 3 01 302 80 91 103 11 1 11 6 12 1 1 35 15 1 16 0 16 7 17 3 18 2 18 8 19 1 203 211 218 ...

Ngày tải lên: 04/06/2014, 15:52

... 4 :10 1 A HHV -6A kD 220- Mw 1- 3 http://www.virologyj.com/content/4 /1/ 1 01 4-7 B 8 -10 11 - 15 16 -17 Pel Mock Mw 1- 3 kD 220- 9766 45- 9766 45- 3020 .11 4.3- 3020 .11 4.3- 4-7 8 -10 11 - 15 16 -17 Pel 220- 2 1. 16 ... (% )1 Virus to host ratio2 dpi Medium #11 15 Purification factor3 88 kD 220 15 8 50 5. 4 5. 2 4 .1 44 kD 88 kD 44 kD 88 kD 44 kD 1. 8 8.3 4.2 0.4 2.0 1. 0 27 11 9 47 4.3 19 7 .5 15 14 11 11 9 .5 7 .5 1Recovery ... Pel 220- 2 1. 16 1. 14 1. 12 1. 1 40 1. 08 1. 06 20 1. 04 1. 02 10 12 14 16 18 Density (g/ml) 1. 18 Viral DNA copies Viral DNA copies Density Density #11 - 15 D Total viral DNA copies (10 6 ) 45- C 66- -gp60...

Ngày tải lên: 18/06/2014, 18:20

... 4 :10 1 A HHV -6A kD 220- Mw 1- 3 http://www.virologyj.com/content/4 /1/ 1 01 4-7 B 8 -10 11 - 15 16 -17 Pel Mock Mw 1- 3 kD 220- 9766 45- 9766 45- 3020 .11 4.3- 3020 .11 4.3- 4-7 8 -10 11 - 15 16 -17 Pel 220- 2 1. 16 ... (% )1 Virus to host ratio2 dpi Medium #11 15 Purification factor3 88 kD 220 15 8 50 5. 4 5. 2 4 .1 44 kD 88 kD 44 kD 88 kD 44 kD 1. 8 8.3 4.2 0.4 2.0 1. 0 27 11 9 47 4.3 19 7 .5 15 14 11 11 9 .5 7 .5 1Recovery ... Pel 220- 2 1. 16 1. 14 1. 12 1. 1 40 1. 08 1. 06 20 1. 04 1. 02 10 12 14 16 18 Density (g/ml) 1. 18 Viral DNA copies Viral DNA copies Density Density #11 - 15 D Total viral DNA copies (10 6 ) 45- C 66- -gp60...

Ngày tải lên: 20/06/2014, 01:20

... t6 Pa t5 Pa t4 Pa t3 N H S Pa t1 LASV IgG Assay NPbac B 3 .5 1/ 100 3.0 1/ 200 2 .5 O.D Pa t2 0.0 2.0 1 .5 1. 0 0 .5 Pa t9 Pa t1 Pa t1 Pa t8 Pa t7 Pa t6 Pa t5 Pa t4 Pa t3 Patient # LASV IgG Assay GP2bac ... Vaccines 2004, 3(2) :10 3 -11 1 McCormick JB: Clinical, epidemiologic, and therapeutic aspects of Lassa fever Med Microbiol Immunol 19 86, 1 75: 15 3 - 15 5 McCormick JB: Epidemiology and control of Lassa ... J Gen Virol 19 91, 72 :54 9 -55 5 Sambrook J, Fritsch EF, Maniatis T: Molecular cloning: a laboratory manual Cold Spring Harbor, Cold Spring Harbor Press; 19 89 References 10 11 12 13 14 Fisher-Hoch...

Ngày tải lên: 20/06/2014, 01:20

... Journal 2006, 3 :58 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 Hatta M, Gao P, Halfmann P, Kawaoka Y: Molecular basis for high virulence of Hong Kong H5N1 influenza A viruses ... binding assay protocol have been described previously [54 ] 15 0 μl binding reactions containing 1 .5 × 1 05 cpm of labelled probe (5' 3'v or 5' 3'c) were incubated with 30 μg of Vac 3P extract for 10 at ... Letters 20 05, 1: 113 -11 7 Engelhardt OG, Smith M, Fodor E: Association of the influenza A virus RNA-dependent RNA polymerase with cellular RNA polymerase II J Virol 20 05, 79 :58 12 -58 18 Smith GL,...

Ngày tải lên: 20/06/2014, 01:20

... reductase (M1 254 6 .1) ; CamB: putidaredoxin (J 054 06 .1) ; CamC: cytochrome P- 450 cam (M1 254 6 .1) ; CamD: 5- exo-alkohol-dehydrogenase (M134 71. 1); CamP: 1, 2-diketocamphane 2 ,5- monooxygenase (AY 450 2 85. 1) ; CamQ: ... putida NCIMB 10 007: Enantioselective preparation of a precursor of Azadirachtin J Chem Soc Perkin Trans 1: 15 0 5– 15 0 5 doi :10 .10 39/p19 950 00 15 0 5 Grogan G, Roberts SM, Wan PWH, Willetts AJ (19 93) Camphor ... purification, and characterization of the recombinant BaeyerVilliger monooxygenase MekA from Pseudomonas veronii MEK700 Appl Microbiol Biot 77 :1 2 51 12 60 doi :10 .10 07/s00 253 -007 -12 64-6 doi :10 .11 86/ 219 1-0 855 -1- 13...

Ngày tải lên: 21/06/2014, 02:20

... 0.0670+0. 012 0a 0.0800+0. 011 a 0.0032+0.0 010 a stem 0.0 15 0 +0.0030b 0.0260+0.0 050 c 0.0 310 +0.009b 0.00 15 + 0.0003c leaves 0.0070+0.0 010 ab 0. 011 0+0.0020bc 0. 011 0+0.004c 0.0007+0.0001bc the whole plant 0. 056 0+0. 012 0** ... environmental science, 2000, 19 (4): 23-26 [11 ] Miu S Y,Chen G Z Pb distribution and migration in simulated mangrove wetland systems [J] China environmental science ,19 98 ,18 (1) :48 - 51 [12 ] Hu G R,Yu R L,Lv ... taken the water samples and plant samples 19 63 Fangli Su et al / Procedia Environmental Sciences 10 (2 011 ) 19 61 – 19 66 Fig .1 Experiment device schemes Sample collection and pretreatment.Water...

Ngày tải lên: 20/07/2014, 12:47

... 11 .9 10 .5 10 .2 10 .5 SiMAG/N-NTA/Nickel 68 .1 His Mre 11 47.6 1. 1 12 .9 15 . 8 6 .5 36.3 76 11 .3 11 .6 64 .5 1. 1 66.2 0.9 2.3 10 .9 5. 5 19 .6 38 31 0 0 0 11 .2 17 .7 3.4 0 .1 0 .1 0 .1 3.7 21 14 33 5. 2 3 .1 1.6 1. 8 ... 1. 8 11 .7 35 21. 3 63.4 8.6 19 .1 9.2 2 .5 39.4 62 24 His Mre 11 50 .6 11 .2 His-ProT 0 .5 SiMAG/CS-NTA/Nickel 65. 7 12 .8 14 .9 3.9 1. 9 33 .5 51 32.2 5. 2 0 .5 12 0 .5 1. 2 4 .1 6.8 57 33 .5 0.8 1. 2 8.9 13 .5 24.4 ... 28.3 5. 4 84.7 94 5. 4 93.3 6 .5 34.2 32 7.7 80.4 86 12 .9 0 .5 26.6 15 . 7 1. 3 0.6 0.3 17 .9 67 8.7 27.2 16 .3 1. 2 1. 1 0.7 19 .3 71 7.9 36.4 1. 3 11 .4 13 .6 1. 2 27 .5 75 8.9 His Mre 11 43 .1 91 .5 His-ProT 11 .9...

Ngày tải lên: 11/08/2014, 00:23

... Porcine Circovirus and Porcine Parvovirus Vet Pathol 2000, 37: 254 -263 Zhou et al Virology Journal 2 010 , 7:366 http://www.virologyj.com/content/7 /1/ 366 10 11 12 13 14 15 16 17 18 19 20 21 22 23 Segales ... 19 86, 12 (1) :1- 6 Casal JI: Use of parvovirus-like particles for vaccination andinduction of multiple immune responses Biotechnol Appl Biochem 19 99, 29 :14 1- 15 0 Casal JI: Parvovirus diagnostic and ... with 5% skimmed milk in TBS-T (50 mM Tris-HCl, 15 0 mM NaCl; 0. 05% Tween 20, pH 7 .5) for h at room temperature (RT) Swine anti-PPV sera (1: 1000 dilution) or anti-His monoclonal antibody (1 :50 00...

Ngày tải lên: 11/08/2014, 21:21

... 95 2 2 72 NaCl 55 50 mM T0 - h+ M 5h C T0 - h+ + T0 h h 5h 15 0 mM 300 mM 95 pH 72 72 55 55 HMS 17 4 75 mM HMS Lys E RP pH 6.0 pH 7.0 pH 8.0 pH 9.0 HMS Rossetta Figure Expression analysis of chimeric ... T0 5h M A T0 T0 5h 5h T0 5h M D 36 37 ºC 21 ºC T0 S S P P 72 28 55 BL 21 Ros2 T0 HMS Ros2 Ros-gami HMS LysE - h+ M 5h B T0 HMS Rossetta - h+ + T0 h h 5h 72 M 55 Ros-gami E BL 21 Ros2 HMS Ros 95 ... Vaccine 20 05, 23:4 011 -40 15 Tregear JW, Roberts LM: The lectin gene family of Ricinus communis: cloning of a functional ricin gene and three lectin pseudogenes Plant Mol Biol 19 92, 18 : 51 5 -52 5 Peumans...

Ngày tải lên: 12/08/2014, 04:21

... Date Run 10 NA 12 0.687 5. 75 NA 0.349 3 .5 NA Discarded NA 0.24 (280 read) 2/ 21/ 2009 0.47 (280 read) 3/6/2009 0.46 (280 read) 4/ 15 / 2009 2/2/2009 2/3/2009 2 /5/ 2009 1. 75 1. 75 38 Figure 11 Immunoprecipitation ... 10 Dialysis 11 Stringent column wash 11 E Protein quantification 11 Bradford assay 11 UV Absorbance 12 F SDS-PAGE and Coomassie ... association has been shown between mPLK/IRAK -1 and TNF R1, and subsequently a physical link has been shown between SIMPL and mPLK/IRAK -1 (Vig et al 19 99; Vig et al 20 01) One goal of this thesis was to identify...

Ngày tải lên: 24/08/2014, 12:43

... families (PR -1 through 17 ) (Van Loon, 19 99; Görlach et al., 19 96; Okushima et al., 2000; Christensen et al., 2002) The PR -1 family consists of proteins with small size (usually 14 -17 kD) and antifungal ... lysozyme and chitinase (Jekel et al., 19 91) The crystal structures of hevamine and its complex with the inhibitor allosamodin has been determined (Terwisscha van Scheltinga et al., 19 94, 19 95, 19 96) ... by Cabib (19 88) with some modifications (Shih et al., 20 01) Each assay reaction mixture contained 50 μL of 0 .1 M sodium acetate buffer (pH5.0), 20 μL of [3H]-chitin (approximately 3x1 05 CPM), various...

Ngày tải lên: 13/11/2014, 09:25

... activity (mg) (U) (U/mg protein) 2 35. 743 387.3 21 1.643 31 . 51 5 1 45. 14 7 4.624 2. 81 3. 810 16 . 758 4.4 2.68 13 Purification (folds) CONCLUSIONS AND SUGGESTIONS 4 .1 Conclusions - Ammonium sulfate fractionation ... Total Specific protein activity activity (mg) (U) (U/mg protein) 2 85. 17 3 14 3. 211 1. 643 61. 840 15 . 754 2.338 1. 42 15 . 3 81 12.400 0.806 0 .5 Purification (folds) Hydrophobic interaction chromatography ... MgSO4.7H2O (0.1g/L), KCl (0.5g/L), FeSO4 (0.01g/L), MnSO4 (0.01g/L), NaCl (0.1g/L), CaCl2 (5g/L), KH2PO4 0 .1% , glucose : sucrose (1: 1) 1% 2.2 Methods 2.2 .1 Preparation of fungi strain and culture...

Ngày tải lên: 06/10/2015, 12:59

... DO in the sediment 10 In ten sity (µV) Intensity(m V ) 50 00 with clams 5cm depth 4000 10 10 10 Molecular weight 10 10 10 10 with clams 15 cm depth 17 6343 409338 3000 2000 10 00 In ten sity µ V ... nitrogen (TAN) The excretion rates of T philippinarum were about 1 .5- 46 .1 mg (kg.clam)-1day -1 and 4.8 -13 1.0 mg (kg.clam)-1day -1 for TAN and TKN, respectively, indicating that NH4-N is the primary ... Intensity ((µV) 50 00 without clams cm depth 4000 without clams 15 cm depth 3000 479364 11 84 816 2000 10 00 0 10 20 30 40 50 60 Retentiontime (min) Retention time (min) 10 20 30 40 50 60 Retention...

Ngày tải lên: 05/09/2013, 09:08