5 molecular analysis of preinvasive breast cancer

... and telomerase levels, an area of extensive study in the field of cancer research We wish to thank the authors of the various chapters of Molecular Analysis of Cancer for their excellent contributions ... Department of Tumour Cell Biology, Institute of Cancer Biology, Danish Cancer Society, Copenhagen, Denmark PER GULDBERG Department of Tumour Cell Biology, Institute of Cancer Biology, Danish Cancer ... Department of Tumour Cell Biology, Institute of Cancer Biology, Danish Cancer Society, Copenhagen, Denmark JESPER ZEUTHEN Department of Tumour Cell Biology, Institute of Cancer Biology, Danish Cancer...

Ngày tải lên: 08/04/2014, 12:51

... Journal of Biomedical Science 2011, 18:36 http://www.jbiomedsci.com/content/18/1/36 Despite the advancement of our understanding of cancer genetics of CRC, genomic alterations of various subtypes of ... colon cancer Cancer Res 2001, 61:900-902 24 Dressler LG, Seamer L, Owens MA, Clark GM, McGuire WL: Evaluation of a modeling system for S-phase estimation in breast cancer by flow cytometry Cancer ... Molecular profile and copy number analysis of sporadic colorectal cancer in Taiwan Journal of Biomedical Science 2011 18:36 Submit your next manuscript to BioMed Central and take full advantage of: •...

Ngày tải lên: 10/08/2014, 05:21

... analysis of p53 56 Gene amplification of hdm2 59 2.4.3 Mutational Analysis of p14ARF 60 2.4.4 Analysis of mRNA expression of p14ARF 61 RESULTS IN HUMAN BREAST CELL LINES 62 2.5.1 Mutational analysis ... Primary breast cancer and corresponding metastatic nodal tissues and normal breast tissues 124 Human breast cancer cell lines RESULTS OF EXPRESSION ANALYSIS 129 132 5.5.1 5.5.2 5.6 Primary breast cancer ... TABLE OF CONTENTS i iii SUMMARY x LIST OF TABLES xiii LIST OF FIGURES xv LIST OF ABBREVIATIONS xx PUBLICATIONS xxiii CHAPTER 1 1.1 GENERAL INTRODUCTION HISTOPATHOLOGY OF BREAST CANCER The normal breast...

Ngày tải lên: 17/09/2015, 17:20

... classification of breast cancer by the WHO (1968) and its prognostic value] Arkh Patol 1980;42(4): 13–19 Singletary SE, Greene FL: Revision of breast cancer staging: the 6th edition of the TNM Classification ... in patients with pT1–2 breast cancer Anticancer Res 2004; 24(5): 3221–3224 Stein RC, Zvelebil MJ: The application of 2D gel-based proteomics methods to the study of breast cancer J Mammary Gland ... 693–699 Early Breast Cancer Trialists' Collaborative Group (EBCTCG) Effects of chemotherapy and hormonal therapy for early breast cancer on recurrence and 15-year survival: an overview of the randomised...

Ngày tải lên: 25/10/2012, 11:15

... dominant inheritance of early onset breast cancer Cancer 73: 643–51 Collaborative Group of Hormonal Factors in Breast Cancer (2001) Familial breast cancer: collaborative reanalysis of individual data ... care of women who are at a raised or high risk of developing hereditary breast cancer Breast cancer is the most common cancer in women Most women with breast cancer not have a family history of ... breast cancer 12 1.2 Breast awareness and examination 1.2.1 Women at increased risk of breast cancer should be breast aware’ in line with Department of Health advice for all women (see www.cancerscreening.nhs.uk/breastscreen/breastaware.pdf)...

Ngày tải lên: 15/03/2014, 00:20

... motif of cardosin A is located at the surface of the protein [4], as seen in other structures of biologically active proteins [32,33] However, a careful examination of the X-ray structure of cardosin ... the tip of a loop protruding away from the core of the protein This amino-acid motif mimics RGD in terms of charge and is positioned at the tip of a loop and is therefore reminiscent of RGD sequences ... superimposition of the 3D structures of kistrin [32] and cardosin A (Fig 8) The importance of both motifs and in particular their basic residues was further emphasized by the complete lack of interaction...

Ngày tải lên: 30/03/2014, 11:20

... an increase of the G1 peack at low dose BPA (30 μM) and an increase of the G2-M fraction of cells at the highest concentration of BPA (100 μM) Differently, in the K562 cells, a model of AML derived ... toxicity of BPA The regulation of caspase-dependent pathways of apoptosis suggests a specific action on the extrinsic and intrinsic pathways of apoptosis which is confirmed by the clear induction of ... combination of ATRA μM and BPA, at the indicated concentrations for 48 hrs (B) Cell cycle analysis and apoptosis in K562 cells after 48 hrs of treatment with 60 and 100 μM BPA (C) Cell cycle analysis...

Ngày tải lên: 18/06/2014, 15:20

... unexpected areas of breast metastases that occurred following the IC infusion of the brain seeking 231BR breast cancer cell line [11] Methods Tumor cell line The 231BR breast cancer cell line ... infusion of cells Region of interest from the head of group Page of 10 (page number not for citation purposes) Journal of Translational Medicine 2009, 7:88 MRI with histological correlation of group ... distribution of breast cancer metastases determined from histology and imaging for the three cohorts of animals was summarized on Table Labeling the 231BRL cells with FEPro did not alter the breast cancer...

Ngày tải lên: 18/06/2014, 15:20

Ngày tải lên: 07/08/2014, 17:22

... and 2) Analysis of hprt mutations in T-cells from spleens of control and test materials-exposed B6C3F1 mice Analysis of the spontaneous hprt mutant clones yielding The toxicologic actions of ozone, ... percentage versus the number of total clones Table DNA sequence analysis of hprt mutant in splenic cells of B6C3F1 female mice in 52-wk study Type of mutation Number of mutants Control Ozone NNK ... expected by addition of their individual responses The study of these interactions can lead to a better understanding of the toxic mechanism of the chemicals involved A number of terms have been...

Ngày tải lên: 07/08/2014, 18:20

... the standard of care in a large population of breast cancer patients A strength is that our study found that African Americans were younger at time of diagnosis of their breast cancer and were ... Frequencies and percentages of women who received standard of care and addition to standard of care are presented in Table Only two-thirds of all breast cancer cases received standard of care No differences ... two-thirds of women in the overall study population received standard of care for their breast cancer While no racial differences were found, many breast cancer cases are not receiving standard of care...

Ngày tải lên: 08/08/2014, 17:20

... breast cancer [21] The risk of subsequent contralateral breast cancer was highest for men aged less than 50 years at the time of the first cancer diagnosis, which is consistent with studies of ... M, Lopez Gomez M, Casado E: Male breast cancer Cancer Treat Rev 2010 Auvinen A, Curtis RE, Ron E: Risk of subsequent cancer following breast cancer in men J Natl Cancer Inst 2002, 94:1330-1332 ... with breast cancer [22,23] Estrogen receptors and progesterone receptors have been suggested to play a role in breast cancers in men, and they are present in about 90% and 81% of breast cancers...

Ngày tải lên: 09/08/2014, 01:24

... review of 31 reported cases of paraneoplastic neurological syndromes due to breast cancer reported only 29% of patients responded to chemotherapy with an improvement in neurological deficits [3] Often ... the nervous system and the underlying cancer Each of these antibodies is associated with a narrow spectrum of clinical syndromes and a restricted subgroup of cancers [2] Paraneoplastic syndromes ... visceral disease Biopsy of the axillary lymph node confirmed the diagnosis of an oestrogen receptor (ER) positive invasive carcinoma of mammary type Mammography and ultrasound of the breasts were unremarkable...

Ngày tải lên: 09/08/2014, 07:22

... Reirradiation of recurrent breast carcinoma of the recurrence and a subgroup of patients (n = 13) are shown Figure Reirradiation of recurrent breast carcinoma Survival proportions of a subgroup of patients ... progression-free start of retreatment; n = 29) cancer Local progression-free survival of reirradiated breast cancer patients (months after start of retreatment; n = 29) A heterogeneous group of patients ... 17:99-107 Bedwinek JM, Fineberg B, Lee J, Ocwieza M: Analysis of failures following local treatment of isolated local-regional recurrence of breast cancer Int J Radiat Oncol Biol Phys 1981, 7:581-585...

Ngày tải lên: 09/08/2014, 09:22

... toxicity Biological tools: To determine breast cancer patients at high risk of local relapse The current locoregional management of early breast cancer consists of breast conserving surgery (i.e., lumpectomy ... MF: The prognostic role of a gene signature from tumorigenic breast- cancer cells N Engl J Med 2007, 356:217-26 van Beers EH, Nederlof PM: Array-CGH and breast cancer Breast Cancer Res 2006, 8:210 ... Fourquet A: Risk of breast cancer recurrence and contralateral breast cancer in relation to BRCA1 and BRCA2 mutation status following breast- conserving surgery and radiotherapy Eur J Cancer 2005,...

Ngày tải lên: 09/08/2014, 10:20

... therapeutic potential of candidate biomarkers, such as IGF-1 for the early detection of breast cancer These studies are important since the breast cancers from populations of women at high risk ... and breast cancer in young women: a pooled re -analysis of three prospective studies Eur J Cancer Prev 2005, 14:493-6 Renehan AG, Frystyk J, Flyvbjerg A: Obesity and cancer risk: the role of the ... BRCA1-associated breast cancer, increased expression of several components of the IGF axis is seen in liver, normal mammary tissue and mammary tumors of these mice along with increased levels of IGF-1...

Ngày tải lên: 11/08/2014, 00:23

... the risk of evolution to cirrhosis and HCC [11] This type of mutants seems to be frequent in our population in view of the fact that 59% of the patients are characterized by the absence of HBeAg ... genotyping In addition to the risk of erroneous results, the limit of the rapid methods using classic PCR is the low sensitivity of DNA detection (the limit of detection of our method being 10 copies/ml) ... constructed using the neighbour-joining algorithm of MEGA4.1 software, with 1000 Bootstrap replicates Results Sixty percent of patients (n = 130 out of 217) were positive by PCR amplification in...

Ngày tải lên: 12/08/2014, 02:20

... integrated analysis of miRNA expression, mRNA expression and genomic changes in human breast cancer and may serve as a basis for functional studies of the role of miRNAs in the etiology of breast cancer ... normal human breast and primary human breast cancer To generate a comprehensive set of miRNA expression profiles for primary human breast cancer we selected 99 primary human tumors, normal breast samples ... classification of human tumors using mRNA microarray profiling is an area of intense research A number of classifiers have been developed for human breast tumors, including the use of expression...

Ngày tải lên: 14/08/2014, 08:20

... of knowledge of the molecular principles of T-DNA transfer process AMT is one of the most powerful genetic techniques for generation of transgenic plants Transgenic plants have a great many of ... the fewer amount of tubulin isoforms and the various functions of MTs in multiple molecular and cellular processes, it was proposed that the function diversity of different types of MTs could be ... synthesis of plant hormones such as auxin or cytokinin, which leads to uncontrolled proliferation of the cells and the formation of tumors The transfer of T-DNA also results in synthesis of opines,...

Ngày tải lên: 09/09/2015, 10:08

... assay of VirE2 in yeast cells 68 Figure 3.22 GFP11 does not perturb the function of VirE2 in AMT of plants 71 Figure 3.23 Study of putative nuclear localization signals of VirE2 in AMT of ... Schematic diagram of Agrobacterium VirE2 labeling strategy 46 Figure 3.9 Schematic diagram of transgenic expression of VirE2 in yeast 48 Figure 3.10 Schematic diagram of internal labeling of VirE2 ... MOLECULAR ANALYSIS OF YEAST FACTOR PMP3P DURING AGROBACTERIUM-MEDIATED TRANSFORMATION LI XIAOYANG (B Sc Science.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BIOLOGICAL...

Ngày tải lên: 09/09/2015, 11:19