5 high dose chemotherapy and allogeneic stem cell transplantation

... Autologous blood stem cell transplantation, High -dose chemotherapy, Outpatient setting, Outpatient supportive care Background High -dose (HD) chemotherapy and autologous blood stem cell transplantation ... Mathias Witzens-Harig1, Anthony D Ho1 and Patrick Wuchter1,4* Abstract Background: High -dose (HD) chemotherapy with melphalan and autologous blood stem cell transplantation (ABSCT) for treatment ... RESEARCH ARTICLE Open Access High -dose chemotherapy and autologous stem cell transplantation of patients with multiple myeloma in an outpatient setting Katharina Lisenko1, Sandra Sauer1, Thomas Bruckner2,

Ngày tải lên: 20/09/2020, 01:31

... fludarabine and days of intravenous busulfan (Flu-ivBu4) regimen between 2006 and 2013 Exclusion criteria were previous allogeneic or cord blood transplantation and ex vivo T cell- depleted stem cell ... Comparison between antithymocyte globulin and alemtuzumab and the possible impact of KIR-ligand mismatch after dose- reduced conditioning and unrelated stem cell transplantation in patients with multiple ... intravenous busulfan and fludarabine: clinical and pharmacokinetic results of a myeloablative, reduced-toxicity conditioning regimen for allogeneic stem cell transplantation in AML and MDS Blood 2004;104(3):857–64

Ngày tải lên: 04/12/2022, 14:56

... blood stem cells for an autologous transplant? Stem Cells Dev 2004;13:598–606 31 Schiller G, Vescio R, Freytes C, et al Transplantation of CD34+ peripheral blood progenitor cells after high -dose chemotherapy ... impact of CD34+ cell dose is marginal, G-CSF significantly accelerates the leukocyte recovery Keywords: Peripheral blood stem cells, Insufficient graft, Autologous stem- cell transplantation, ... transplantation, Multiple myeloma Background High -dose (HD) chemotherapy followed by autologous blood stem- cell transplantation (ASCT) is the standard of care and a highly effective therapy for multiple

Ngày tải lên: 30/05/2020, 21:57

... Hematopoietic stem cell transplantation B-thalassemia major Fanconi’s anemia Immunodeficiency diseases Inherited metabolic disorders A B S T R A C T Allogeneic hematopoietic stem cell transplantation ... prolonged survival ª 2014 Production and hosting by Elsevier B.V on behalf of Cairo University Introduction Patients and methods Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is a ... at a total dose of 110 mg/kg divided into 10 doses (5 pre- and post-transplant) FA patients received Flu/Cy/ ATG regimen consisting of low -dose cyclophosphamide mg/kg/day for days and fludarabine

Ngày tải lên: 15/01/2020, 06:21

... after the start of infusion for dose 1, dose and dose 9/HPLC-UV 20 min, 40 as well as 1, 1.5, 2, 3, 4, and h after the start of infusion for dose 1, dose and dose 9/GC-MS po 40 mg/m2 po mg/kg ... Sampling and analysis 0.5, and h after the start of infusion for dose 1; h after the start of infusion for dose 2, 3, 4, 12,13/GC-MS 0, 1, 2, 2.25, 2.5, and h after the start of infusion for doses and ... 2.25 and h after the start of infusion for doses13/GC-MS 2,3,4,5,6 h after the start of infusion for doses and 9;2 and h after the start of infusion for doses 13 /GC-MS NR/GC-MS 1, 2.25, 2.5, and

Ngày tải lên: 29/05/2020, 18:56

... self-renewing, stem- like cells within tumors, which have been called cancer stem cells (CSCs) [23] Increasing evidence suggests that EMT bestows carcinoma cells at the tumor front with cancer stem cell ... molecular process through which cell- to -cell interactions and apico-basal polarity are lost and a mesenchymal phenotype is acquired, which are required for cell motility and basement membrane invasion ... and cancer stem cell- like properties in SCC9 cells Cong-Chong Yang1,2, Li-Fang Zhu3, Xiao-Hui Xu1,4, Tian-Yun Ning1,2, Jin-Hai Ye1,5 and Lai-Kui Liu1,2* Abstract Background: Tissue invasion and

Ngày tải lên: 05/11/2020, 07:37

... affects the morphology, division and differentiation of stem cells Cells, such as differentiating embryonic stem cells, spread to a greater degree and form prominent intracellular actin stress fibres ... could direct bone marrow stromal stem cells (alternatively referred to as mesenchymal stem cells, MSCs) to differentiate into cells as diverse as neurons, muscle and bone (Engler et al., 2006) ... promote cell division (Peyton et al., 2006; Yeung et al., 2005), stimulate tumour growth and fibrosis (Georges and Janmey, 2005; Li et al., 2007) and direct the differentiation of other stem cells,

Ngày tải lên: 02/11/2022, 09:35

... Van Pham et al Stem Cell Research & Therapy 2013, 4:91 http://stemcellres.com/content/4/4/91 RESEARCH Open Access Activated platelet-rich plasma improves adipose-derived stem cell transplantation ... myogenic, and osteogenic cells in the presence of specific induction factors [12] These cells are termed adipose-derived stem cells (ADSCs) ADSCs express surface markers as CD44, CD73, CD90, and CD105, ... Adipose-derived stem cell culture SVF cells were cultured to expand the number of ADSCs SVF cells were cultured in DMEM/F12 (Sigma-Aldrich, St Louis, MO, USA) containing 1× antibiotic–mycotic and 10%

Ngày tải lên: 12/12/2017, 05:46

... undergoing hematopoietic stem cell transplantation? ??HSCT）before（Time 1［T1］ ? ?and one month after transplantation? ??Time 2［T2］ ） , and? ??2）to identify the pre-HSCT factors that predict anxiety and depressive ... before and one month after transplantation, and2 to identify the pre-HSCT factors that predict anxiety and depressive symptoms in fathers and mothers one month after transplantation Patients and ... high -dose chemotherapy and total body irradiation Blood Cell Therapy-The official journal of APBMT-Vol Issue No 2019 39 40 Blood Cell Therapy-The official journal of APBMT-Vol Issue No 2019 TBIand

Ngày tải lên: 15/01/2020, 06:10

... sepsis and fungemia, sepsis and viremia, and sepsis and bacteremia Moreover, cytokine IL-8 enabled a significant differentiation between sepsis and viremia, sepsis and bacteremia, and bacteremia and ... bacterial, viral and fungal infections are known as adverse events after hematopoietic stem cell transplantation representing the major cause for morbidity and mortality Detection and differentiation ... autologous transplantation (n = 2) All autologous and allogeneic transplant recipients received standard prophylaxis including antimycotics, virostatics and metronidazole On day four after HSCT, all allogeneic

Ngày tải lên: 28/09/2020, 01:35

... unresponsive immune system with a new, tumor-sensitive immune system by allogeneic hematopoietic stem cell transplantation (alloHSCT) Transplantation of allogeneic hematopoietic stem cells from a human-leukocyte-antigen ... mg/kg total dose) Stem cell product and stem cell transplantation Only mobilized hematopoietic stem cells obtained from peripheral blood by hemapheresis according to § 13 AMG and the German Transfusion ... surgical resection and standard adjuvant chemotherapy Methods/Design: The STEM PACE trial is a single center, phase II study to evaluate adjuvant allogeneic hematopoietic stem cell transplantation

Ngày tải lên: 05/11/2020, 01:33

... England Journal of Medicine From www.bloodjournal.org by guest on February 21, 2017 For personal use only Gennery AR, Slatter MA, Grandin L, et al Transplantation of hematopoietic stem cells and ... sources and conditioning regimens, or use of hematopoietic stem cell infusion alone may result in different outcomes, depending on the immunophenotype and genotype 9,10 Tlymphocyte donor chimerism and ... CD27/CD45RA15 and CD16/56 enumeration and serum IgG, IgA, and IgM levels were measured routinely Donor cell chimerism was based on polymerase chain reaction amplification of short tandem repeats

Ngày tải lên: 04/12/2022, 15:13

... high -dose IL-2 to determine the safety, objective response rate and effect on humoral and cell- mediated immunity. Methods: 25 patients with metastatic RCC who qualified for IL-2 were eligible and ... from the cell membrane and thus can medi- ate antibody-dependent cell- mediated cytotoxicity (ADCC). In addition, 5T4-transduced renal carcinoma cell lines can be recognized by human T cells in ... high -dose IL-2. We fur- ther characterized the effector CD8+ T cells in whole PBMC and found that there was an increase in CD107a, a marker of degranulation and cytotoxic function [16,17]. These cells

Ngày tải lên: 18/06/2014, 15:20

... forward and side scatter properties, and T cells were identified based on their expression of CD4 and CD3 To discriminate between CD25high Treg and CD25low activated effector-memory T cells, we ... factor-b (TGF-b), IL-10, and autoantibody levels were also measured, along with efficacy and safety Patients and methods Patients Patients with stage III and IV operable and inoperable melanoma ... and CD3+ cells and significantly greater decreases in endotumoral CD83+ cells compared with nonresponders However, a recently published subanalysis of the European Organization for Research and

Ngày tải lên: 18/06/2014, 16:20

... irradiated to 40 Gy, and to 66 Gy, was 18 cm, and 12 cm, respectively. All patients were treated with 10 MV photon beams. Chemotherapy Two schedules of chemotherapy were used. Concurrent chemotherapy ... with high -dose accelerated radiotherapy with concomitant chemotherapy, with sequential chemotherapy or without chemotherapyFigure 4 Actuarial overall survival of patients treated with high -dose ... multi-agent standard chemotherapy sched- ules with higher cumulative doses of cisplatin than in our study except for the EORTC trial. There is no convincing evidence that concomitant stand- ard poly-chemotherapy

Ngày tải lên: 09/08/2014, 10:21

... Marrow Transplantation Organ < 21 days > 21 days Mouth Mucositis Infection: Candida, herpes simplex Infection: Candida, herpes simplex GVHD: acute & chronic Xerostomia Mucositis Infection: Candida, ... correction of fluid and electrolyte abnormalities and on preventing or correcting resultant malnutrition and nutrient deficiencies c) Diarrhea interferes with the absorption of oral CsA and may require ... immunologic standpoint, GVHD is initiated when: a) Genetically determined histocompatibility differences exist between the bone marrow recipient and the bone marrow donor b) Immunocompetent cells in

Ngày tải lên: 10/08/2014, 08:20

... WiCell Research Institute, National Stem Cell Bank In this protocol, the hESCs formed embryoid bodies using non-adherent cell culture plates and were transferred onto laminincoated plates and ... differentiation state or potency of stem cells in comparison to differentiated cells, DNA methylation studies were done The goal was to identify methylation changes between hES cells grown on the human ... their high nuclear to cytoplasm ratio, but there were more pockets and areas consisting of differentiated cells with flatter and larger morphologies (arrows) 3.6.4 Pluripotency markers levels

Ngày tải lên: 11/09/2015, 10:07

... blood stem cell transplantation; BMT, bone marrow transplantation; UBMT, unrelated bone marrow transplantation; T-UBMT, T -cell- depleted bone marrow transplantation; UCBT, umbilical cord blood transplantation; ... Biology of Blood and Marrow Transplantation, 13, 1207-1215 Joseph, R W., Couriel, D R., & Komanduri, K V (2008) Chronic graft-versus-host disease after allogeneic stem cell transplantation: Challenges ... ease after allogeneic hematopoietic stem cell transplantation Biology of Blood and Marrow Transplantation, 8, 674-682 Richards, S J., Morgan, G J., & Hillmen, P (1999) Analysis of T cells in paroxysmal...

Ngày tải lên: 12/02/2014, 19:20

... angiogenesis and vasculogenesis: Cellular approach Adult mesenchymal stem cells Smooth muscle cells Hematopoietic stem cells and progenitor cells Endothelial progenitor and endothelial cells Bone ... exogenous stem cells and differentiated cells and also by enhancing the mobilization of endogenous stem cells These cells have natural capacity of participating in therapeutic angiogenesis and vasculogenesis ... Low dose: 0.005 and 0.017µg/kg High dose: 0.05 and 0.167µg/kg Coronary artery disease 15 I Low dose: 17ng/kg High dose: 50ng/kg Coronary artery disease 178 II Reference Dose Hendel et al, Low dose: ...

Ngày tải lên: 14/09/2015, 09:26

... established two Ptb) ⁄ ) ES cell lines, and found that Ptb) ⁄ ) ES cells showed severe defects in cell proliferation in vivo and in vitro (Figs 3B and 4B) As Ptb) ⁄ ) ES cells exhibit a low proliferation ... blue-staining-negative cells to that of total cells Cells (3 · 105) were seeded on growth medium and counted every day for days of culture The circles indicate the values for wild-type cells and the triangles ... undifferentiated state and did not lead to precocious differentiation PTB in development and ES cells 2.5 1.5 0.5 +/+ –/– Fig Ptb) ⁄ ) ES cells have a severe defect in cell proliferation in vivo and in vivo...

Ngày tải lên: 07/03/2014, 00:20