... was a band of about 29 kDa on the SDS- Page of Figure The expression of GST-ORF2-E protein was analyzed by SDS-PAGE (A) and Western-blotting (B) with an anti-GST monoclonal antibody Lane 1, BL21 ... induction of IPTG; Lane 2, BL21 cell lysate after induction of IPTG; Lane 3, Supernatant of cell lysate after sonication and centrifugation; Lane 4, Pellet of cell lysate after sonication and centrifugation, ... membrane using an anti-GST monoclonal antibody and porcine serum Evaluation of GST-ORF2-E proteins ELISA Figure (A) The map of dominant immunoreactive areas of ORF2 The amino acid residues of each...
Ngày tải lên: 12/08/2014, 01:22
Ngày tải lên: 07/11/2013, 09:15
AN INTEGRATED ATOM CHIP FOR THE DETECTION AND MANIPULATION OF COLD ATOMS USING a TWO PHOTON TRANSITION
... Aarthi, Daniel and Siva, thank you a lot for working towards the chip design and fabrication It was a nice time to get a chance to know each other and work together Johnathan and Nillhan it was ... field, which made my many wrong calculation right, many doubts clear and many exams to pass together! Thanks for the proof-reading of my thesis and for your patience and support Thanks SINGAPORE! v ... + z −xz 21 (2.25) THEORY OF COOLING AND TRAPPING OF ATOMS ON AN ATOM CHIP where the x-axis is the longitudinal symmetry axis and the y − z plane is the transverse plane Around the trap center,...
Ngày tải lên: 08/09/2015, 15:07
Evaluating the Reliability and Validity of an English Achievement Test for Third-year Non- major students at the University of Technology, Ho Chi Minh National University and some suggestions for chan
... good tests can sustain or enhance class morale and aid learning 2.2 Language Testing Language testing is one of the forms of testing and it is also one form of measurements Its importance in English ... that reliability and validity are essential to the interpretation and use of measures of language abilities and are the primary qualities to be considered in developing and using tests For this ... to the list of numbers that occur most frequently Thus, in this set of numbers, there are two modes, which are and Measures of Dispersion: the standard deviation (sd) and the range Range is the...
Ngày tải lên: 07/11/2012, 15:05
Isolation and characterization of an extracellular polymer closely related to flocculation of activated sludge
... Blumenkrantz, N and Asboe-Hansen, G (1973) New method for quantitative determination of uronic acids Anal Biochem., Vol.54, 484-489 Dermlim, W., Prasertsan, P and Doelle, H (1999) Screening and characterization ... determined using the method of Blumenkrantz and Hansen (1973) with a glucuronic acid/galacturonic acid 1:1 standard Hexosamine was determined by the method of Blix (1948) with a glucosamine standard ... as follows: 500 g of soil was washed times with distilled water and suspended in 1,000 ml of water The mixture was allowed to stand for 10min and the supernatant was collected and used for the...
Ngày tải lên: 05/09/2013, 09:08
Real-Time Digital Signal Processing - Chapter 5: Design and Implementation of FIR Filters
... Magnitude response of bandpass filter with narrow bandwidth 188 DESIGN AND IMPLEMENTATION OF FIR FILTERS where !a and !b are the lower and upper cut-off frequencies, respectively The bandwidth is the ... response of an ideal bandstop (or band-reject) filter is illustrated in Figure 5.1(d) The regions ! !a and ! ! !b are referred to as the passband The region !a < ! < !b is called the stopband A bandstop ... frequency and the stopband edge frequency, respectively 186 DESIGN AND IMPLEMENTATION OF FIR FILTERS H(w) Ap 1+d p Ideal filter 1−d p Actual filter As ds Passband p wc ws wp Transition band w Stopband...
Ngày tải lên: 23/10/2013, 22:15
Tài liệu The Life and Times of an Object pdf
... only the compiler can make this translation You can't override Finalize yourself and you can't call Finalize yourself Why Use the Garbage Collector? In C#, you can never destroy an object yourself ... object in the same piece of memory Either way, the outcome of using dangling reference would be undefined All bets would be off You'd try and destroy the same object more than once This might or ... starting to run low), and then it collects as much as it can Performing a few large sweeps of memory is more efficient than performing lots of little dustings! NOTE You can invoke the garbage...
Ngày tải lên: 15/12/2013, 00:15
Analysis and design of control systems using MATLAB
... inverse tangent of x The function returns an angle in radians between – π/2 and π/2 atan(x) atan2(y, x) Computes the arctangent or inverse tangent of the value y/x The function returns an angle ... A Command Window which is used to enter commands and data to display plots and graphs A Graphics Window which is used to display plots and graphs An Edit Window which is used to create and modify ... sensitivity of the ratio of output to input for variations in system characteristics and other parameters (c) Reduced effects of nonlinearties and distortion (d) Increased bandwidth (bandwidth of a...
Ngày tải lên: 01/01/2014, 18:43
Tài liệu Mountain Goats Ecology, Behavior, and Conservation of an Alpine Ungulate doc
... winter ranges north and east of Caw Ridge are under increasing pressure from logging activities and oil and gas exploration (Smith 2004; Smith et al 2000), and the future of woodland caribou in Canada ... alpine: it changes rapidly and is generally cold and often windy Weather is a major factor affecting fieldwork and data collection Snowfalls of 20 cm or more and temperatures of –4°C or less can happen ... Questions, Conservation Challenges, and Long-Term Research The conservation of biodiversity and the management of wildlife require an understanding of the basic ecology of animals That deceptively simple...
Ngày tải lên: 17/02/2014, 17:20
Tài liệu Báo cáo khoa học: Identification and characterization of an R-Smad ortholog (SmSmad1B) from Schistosoma mansoni pdf
... arthropods, and vertebrates The same results were obtained by maximum likelihood and neighbor-joining distance analyses (supplementary Figs S1 and S2) SmSmad1B gene structure and 5¢ upstream analysis ... of SmSmad2 and SmSmad4 and resulted in the formation of a stable and functional Smad complex [6,7] The above results are consistent with the report that human GCN5 interacts with both TGFb- and ... Cotransformation numbers and were streaked in duplicate (B) LacZ filter-lifts from transformed yeast grown on SD media lacking leucine and tryptophan Table Summary of the yeast two-hybrid and...
Ngày tải lên: 18/02/2014, 16:20
Tài liệu Báo cáo khoa học: "Collecting a Why-question corpus for development and evaluation of an automatic QA-system" pdf
... dynamic and inexpensive method of collecting a corpus of questions and answers using the Amazon Mechanical Turk framework We have provided to the community a corpus of questions, answers and corresponding ... 2005; Soricut and Brill, 2006), as have the human-powered question sites such as Answers.com, Yahoo Answers and Google Answers, where individuals can post questions and receive answers from peers ... finegrained segmentation and for our purpose, much of the HTML-information was redundant anyway Consequently we removed most of the HTMLstructures, and the table-cells, list-items and other similar...
Ngày tải lên: 20/02/2014, 09:20
Therapeutic ultrasound as a potential male contraceptive: power, frequency and temperature required to deplete rat testes of meiotic cells and epididymides of sperm determined using a commercially available system doc
... GJM and DCS analyzed data and directed Pilot Studies and Study 1; MAS treated animals, performed necropsies and supervised all animal experiments performed at ILS; EJRS and KGH treated animals, ... treated animals, analyzed histology, analyzed sperm parameters, and wrote the manuscript; PAD, CMG, RCG, and TSG designed, performed or analyzed in vitro ultrasound experiments, MGO, PAD, GJM and ... the transducer mimics its use as a therapeutic device and results in an averaging of the field output over time The distance between the transducer and the scrotum was initially set to cm In an...
Ngày tải lên: 05/03/2014, 17:20
Báo cáo khoa học: "Learning Arguments and Supertypes of Semantic Relations using Recursive Patterns" doc
... pattern X and Y work for Z X and Y fly to Z X and Y go to Z X and Y work in Z X and Y work on Z X and Y work at Z X and Y live in Z X and Y live at Z X and Y live with Z X and Y cause Z X and Y celebrate ... 1998), syntactic dependencies (Pantel and Ravichandran, 2004), and lexico-syntactic patterns (Riloff and Jones, 1999; Fleischman and Hovy, 2002; Thelen and Riloff, 2002) When research focuses ... than DAP that use only one seed to harvest the arguments and supertypes of a wide variety of relations (Banko and Etzioni, 2008) show that semantic relations can be expressed using a handful of...
Ngày tải lên: 07/03/2014, 22:20
Synthesis and characterization of silicon nanowires using tin catalyst for solar cells application
... difference of the reflectance might be the effect of synthesis conditions, such as thickness of metal film and SiH4 gas flow The reduction of reflectance is very helpful to improve the solar cell performance ... reduction of the contact resistance and optimization of the nanowire sizes will be considered and the results will be presented near future References [1] [2] [3] [4] [5] Fig (a) Photograph of the ... difference of reflectance between conventional planar c-Si solar cell and SiNWs solar cell the Schottky barrier between the evaporated Al contact and silicon wafer because of insufficient annealing...
Ngày tải lên: 16/03/2014, 15:12
Báo cáo khoa học: Isolation and characterization of an IgNAR variable domain specific for the human mitochondrial translocase receptor Tom70 potx
... bond In an initial series of experiments, recombinant 12F-11 protein was denatured and reduced using guanidine HCl and dithiothreitol, followed by alkylation of the cysteine residues and refolding ... replacement of residues Cys22 and Cys82 with alanine and valine, respectively, to give a cysteine minus mutant (12F-11DCys) Use of alanine and valine was initially determined in a set of competitive ... incorporation of cysteine at CDR3 loop residue positions and 7–11, to enhance the possibility of inter- and intra CDR disulphide cross-links These strategies are summarized in Fig 1B and Table 1, and details...
Ngày tải lên: 17/03/2014, 10:20
Báo cáo Y học: Puri®cation and properties of an alkaline proteinase of Fusarium culmorum doc
... azogelatin assay at 40 °C and pH 3.6, 4.1, 4.5, 5.0 and 5.5 (Na acetate), pH 6.0, 6.5 and 6.9 (Na citrate), pH 6.9, 7.5, 7.9, 8.3, 8.7 and 9.1 (Tris/HCl) and pH 9.2, 9.6, 10.0 and 10.4 (Caps) All buffers ... buffer and 200-drop fractions were collected The absorbance values of these and of the chromatography fractions in subsequent steps were measured at 280 nm and their nonspeci®c proteinase and chymotrypsin/subtilisin-like ... preparations contained a Fig SDS/PAGE pattern of the puri®ed proteinase Lanes and 2: 0.5 and 1.5 lg of CM-HPLC puri®ed enzyme; lane 3: molecular mass standards protein of mass 28 663 50 Da Some puri®ed...
Ngày tải lên: 31/03/2014, 21:21
the development and application of an antibody based biosensor
... Prize winner, Karl Landsteiner investigated many of the fundamental principles of immunochemistry using an elegant molecular level approach to understanding antibody recognition (Landsteiner 1962) ... ancient remains of marine plant and animal life under extreme heat and pressure in an anaerobic environment Depending on the composition of the organic material, varying proportions of aliphatic ... 58 Synthesis and mass spectrum of 3TAA 59 Synthesis and mass spectrum of 5M2TAA 60 Synthesis and mass spectrum of 2MF9AA 61 Synthesis and mass spectrum of 3MF9AA ...
Ngày tải lên: 13/05/2014, 14:01
Báo cáo sinh học: "Construction and characterization of an infectious clone of coxsackievirus A16" docx
... development and testing of candidate live attenuated vaccines and antiviral therapeutics against CVA16 Methods Cells and viruses RD and Vero cells were grown in DMEM (Gibco, Grand Island, NY, USA) ... pig anti-CVVP0 (A–C and M–O), antiCVVP1t (D–F and P–R), anti-CVVP3 (G–I and S–U), or pre-immune serum (J–L and V–X), followed by incubation with a FITC-conjugated goat anti-guinea pig IgG antibody ... band represented RNA transcripts produced from the cDNA clone The resultant transcripts were used to transfect RD cells At 72 h post-transfection, cells and supernatants were harvested and analyzed...
Ngày tải lên: 18/06/2014, 18:20
Báo cáo hóa học: " Development and validation of an Eating Disorders Symptom Impact Scale (EDSIS) for carers of people with eating disorders" doc
... receiving treatment, and the scales of EDSIS, ECI-negative and GHQ-12 were low, and most were non significant Significant correlations were found between type of diagnosis and Dys- Table 3: Correlations ... conceived and designed the study, oversaw all stages of data collection and analysis, and drafted the manuscript JW conducted focus groups, provided clinical advice on design and did qualitative analysis ... researchers based on quantitative and qualitative work with carers and reviewed by a panel of "expert carers" [12,16,26,27] The panel was made up of a psychiatrist (one of the authors of this paper),...
Ngày tải lên: 18/06/2014, 22:20
Báo cáo hóa học: " Construction and characterization of an infectious clone of coxsackievirus A16" ppt
... development and testing of candidate live attenuated vaccines and antiviral therapeutics against CVA16 Methods Cells and viruses RD and Vero cells were grown in DMEM (Gibco, Grand Island, NY, USA) ... pig anti-CVVP0 (A–C and M–O), antiCVVP1t (D–F and P–R), anti-CVVP3 (G–I and S–U), or pre-immune serum (J–L and V–X), followed by incubation with a FITC-conjugated goat anti-guinea pig IgG antibody ... band represented RNA transcripts produced from the cDNA clone The resultant transcripts were used to transfect RD cells At 72 h post-transfection, cells and supernatants were harvested and analyzed...
Ngày tải lên: 20/06/2014, 01:20