4viral modulation of nf kappa b activity

Tài liệu Báo cáo khoa học: Autophagy inhibits reactive oxygen species-mediated apoptosis via activating p38-nuclear factor-kappa B survival pathways in oridonin-treated murine ﬁbrosarcoma L929 cells doc

... inhibitor kappa B (I-jB) level, with the increase of phosphorylated I-jB (p-I-jB) and NF- jB levels being time-dependently indicative of NF- jB activation Interestingly, we found that inhibition of ... cells, NF- jB is sequestered in the cytoplasm by binding to the inhibitor of NF- jB (I-jB) In response to a variety of stimuli, activation of NF- jB typically involves the phosphorylation of I-jB, resulting ... we found that inhibition of NF- jB activation by using NF- jB inhibitor pyrrolidine dithiocarbamate (PDTC) or speciﬁc siRNA, which caused a reduction in NF- jBp65 levels (Fig 6B) , signiﬁcantly increased...

Ngày tải lên: 18/02/2014, 13:20

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Tài liệu Báo cáo khoa học: Staphylococcal enterotoxin C1-induced pyrogenic cytokine production in human peripheral blood mononuclear cells is mediated by NADPH oxidase and nuclear factor-kappa B doc

... translocation of NF- jB which may bind to its target genes to trigger the production of IL- 1b and IL-6 that is blocked by the NF- jB inhibitor (PDTC or SN-50) Moreover, the NF- jB binding ability is abrogated ... monoclonal NF- jB p65, rabbit polyclonal NF- jB p65, goat and rabbit polyclonal NF- jB p50, and rabbit polyclonal p47phox were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA) Rabbit polyclonal ... p47phox (Fig 5B) , translocation of NF- jB (Fig 2C), DNA-binding activity of NF- jB (Fig 2D) and phosphorylation of IKK -b, IjBa (Fig 4), whereas inhibitors of NF- jB not affect the ROS generation...

Ngày tải lên: 19/02/2014, 00:20

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Báo cáo y học: "The S100A8/A9 heterodimer amplifies proinflammatory cytokine production by macrophages via activation of nuclear factor kappa B and p38 mitogen-activated protein kinase in rheumatoid arthritis" potx

... with 100 ng/ml of lipopolysaccharide were used as positive controls The specificity of NF- B protein binding was verified by inhibition and supershift experiments with unlabeled NF- B consensus ... and anti -NF- B p65 antibody (b) Effects of p38 MAPK inhibition on S100A8/A9-induced NF- B activation Monocytes, after two-hour pretreatment with or without the MAPK inhibitors (PD98059, SB202190, ... DNA-binding activity of NF- B by EMSA As shown in Figure 7a, the NF- B activity was induced by S100A8/A9 in a dose-dependent manner, verifying the activation of NF- B by S100A8/A9 To further determine...

Ngày tải lên: 09/08/2014, 07:20

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Báo cáo y học: " Nontypeable Haemophilus influenzae induces COX-2 and PGE2 expression in lung epithelial cells via activation of p38 MAPK and NF-kappa B" pps

... pre-treatment of the specific inhibitor of p38 MAPK SB203580 blocked p38 MAPK signaling activated by NTHi (Fig 4B) Up-regulation of NF- kappa B DNA binding activity after NTHi stimulation Since NF- kappa B ... binding activity of NF- kappa B induced by NTHi A549 cells were incubated with different concentrations of NTHi with or without SB203580, and DNA binding activity of NF- kappa B was measured by EMSA ... NTHi-induced NF- kappa B signaling pathway EMSA analysis showed that NFkappa B translocation and its DNA binding activity were markedly increased by NTHi within 90 mins, and DNA binding activity of NF- kappa...

Ngày tải lên: 12/08/2014, 15:21

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Báo cáo y học: "Potential role and mechanism of IFN-gamma inducible protein-10 on receptor activator of nuclear factor kappa-B ligand (RANKL) expression in rheumatoid arthritis" pptx

... membrane was subsequently washed and incubated with a peroxidase-labeled rabbit anti-goat IgG antibody for hours at room temperature and then incubated with an enhanced chemiluminescence substrate ... inhibition of Gai by PTX was confirmed by measuring CREB phosphorylation that was increased following Gai inhibition Figure shows that CXCL10 augmented the expression of RANKL more than twofold ... seven-transmembrane receptor and has heterotrimeric G proteins consisting of a, b, and g subunits The G protein resides attached to the Figure Induction of receptor activator of nuclear factor kappa- B ligand...

Ngày tải lên: 12/08/2014, 17:22

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Tài liệu Báo cáo khoa học: Polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells pdf

... Magee BH, Markert M & Babior BM (1986) The FEBS Journal 272 (2005) 444–453 ª 2004 FEBS M Rumbo et al respiratory burst oxidase of human neutrophils Further studies of the purified enzyme J Biol ... iNOS binds Rac2, a member of the Rho GTPase family, and overexpression of Rac2 leads to a specific distribution of iNOS to the insoluble fraction This effect is accompanied by increased iNOS activity ... redistribution and loss of activity FEBS Journal 272 (2005) 444–453 ª 2004 FEBS M Rumbo et al Targeting iNOS activity to specific cellular domains is independent of stimulation as a similar distribution...

Ngày tải lên: 19/02/2014, 16:20

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Báo cáo khóa học: Disruption of the interaction between the Rieske iron–sulfur protein and cytochrome b in the yeast bc1 complex owing to a human disease-associated mutation within cytochrome b potx

... detail below Instability of the mutant enzyme Further analysis of the kinetic parameters of the G167E mutant was hindered by rapid inhibition of the activity of Fig QH2-cytochrome c reductase activity ... concentration of bc1 complex in the activity assay was 2.5 nM (A) Activity of the mutant enzyme as a function of the substrate QH2 concentration (B) The rate of cytochrome c reduction by wild type ... could be excluded, because the addition of an equal amount of decylubiquinone to decylubiquinol at the beginning of the assay had no effect on the kinetics (data not shown) It is more probable...

Ngày tải lên: 07/03/2014, 15:20

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Báo cáo khoa học: Interaction of human steﬁn B in the preﬁbrillar oligomeric form with membranes Correlation with cellular toxicity doc

... stefin B prefibrillar oligomers of vesicles made of acidic phospholipids resembles pore formation by A -b [27] and liposome permeabilization of a-synuclein [43] The toxic activity exerted by prefibrillar ... composed of loosely bound oligomeric blocks can be seen and, at pH 3.3 (Fig 1B) , necklacelike structures built from basic ellipsoid blocks (similar to protofibrils) are observed At pH 7.3, oligomers of ... Condron MM, Lomakin A, Benedek GB, Selkoe DJ & Teplow DB (1999) Amyloid beta-protein fibrillogenesis Structure and biological activity of protofibrillar intermediates J Biol Chem 274, 25945–25952...

Ngày tải lên: 07/03/2014, 17:20

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Báo cáo khoa học: Regulation of DNp63a by tumor necrosis factor-a in epithelial homeostasis docx

... 4A) The nuclear translocation of p65, a subunit of NF- jB, also required both TNF-a and cycloheximide (Fig 4B) Treatment of the NF- jB inhibitor JSH23 inhibited both IjBa degradation and p65 nuclear ... that cycloheximide may be required for the efﬁcient degradation of inhibitor of kappa B (IjBa) and hence activation of NF- jB [32] Therefore, we examined the levels of IjBa as well as p65 translocation ... TNF-a was attenuated by inhibition of NF- jB, suggesting that activation of NF- jB may be involved in the regulation of the degradation of DNp63a Interestingly, knockdown of DNp63a expression in DNp63aexpressing...

Ngày tải lên: 16/03/2014, 06:20

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Báo cáo khoa học: Transgenic Cdx2 induces endogenous Cdx1 in intestinal metaplasia of Cdx2-transgenic mouse stomach pot

... GCCGGGGTGGGG Briefly, 0.5 ng of 32P-labeled probe was incubated for 20 at °C with lg of nuclear extracts in the presence of · gel shift buffer (Promega) Subsequently, 1.5 lL of 10· loading buffer were ... indeed, the Cdx1 and ⁄ or Cdx2 homeoproteins were found to be able to bind to the TATA-boxes of some intestinal genes, such as those of the calbindin-D9 gene [21], the clusterin gene [22], and the ... gene promoter CpGs is best analyzed by using direct sequencing after sodium bisulfite modification of target DNA (bisulfite sequencing) DNA (1 lg of DNA per sample) was sodium bisulfite modified with...

Ngày tải lên: 23/03/2014, 04:20

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Báo cáo khoa học: Transport of the phosphonodipeptide alafosfalin by the H+/peptide cotransporters PEPT1 and PEPT2 in intestinal and renal epithelial cells doc

... (1980) Eﬀect of food on the bioavailability of alafosfalin, a new antibacterial agent J Antimicrob Chemother 6, 373–379 Ó FEBS 2004 Kafarski, P & Lejczak, B (2001) Aminophosphonic acids of potential ... cells as described previously [24,26] (see below) Alafosfalin showed a remarkably high Fig Inhibition of [14C]Gly-Sar uptake by alafosfalin in Caco-2 cells (A) and SKPT cells (B) Uptake of 10 lM [14C]Gly-Sar ... Metab Dispos 29, 1307–1315 Ó FEBS 2004 Alafosfalin transport by H+/peptide transporters (Eur J Biochem 271) 2017 26 Borner, V., Fei, Y.-L., Hartrodt, B. , Ganapathy, V., Leibach, ¨ F.H., Neubert,...

Ngày tải lên: 23/03/2014, 12:20

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Báo cáo khoa học: Nuclear factor kappa B and tumor necrosis factor-alpha modulation of transcription of the mouse testis- and pre-implantation development-speciﬁc Rnf33⁄Trim60 gene pot

... The Rnf33 jB element is targeted by the NF- jB proteins p50 and p65 It has been well established that jB sequences are targeted by the abundantly expressed NF- jB [13–15] Involvement of the NF- jB ... hypoxia-inducible factor-1alpha by NF- kappaB Biochem J 412, 477–484 NF- jB modulates testis-speciﬁc Rnf33 expression 12 Gorlach A & Bonello S (2008) The cross-talk between NF- kappaB and HIF-1: ... modulated by the NF- jB subunits p65 and p50, and also by TNF-a Although p65 is probably expressed in the oocyte and the pre-implantation embryo, it remains to be shown if the TNF-a ⁄ NF- jB signaling...

Ngày tải lên: 29/03/2014, 00:20

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Báo cáo khoa học: Effect of 5-lipoxygenase inhibitor MK591 on early molecular and signaling events induced by staphylococcal enterotoxin B in human peripheral blood mononuclear cells doc

... Effects of SEB on human PBMCs C Mendis et al Effect of 5-LO inhibitor MK591 on monkeys challenged with SEB Findings of in vitro studies of human PBMCs were veriﬁed in PBMCs isolated from aerosol SEB ... inhibitor to block 5-LO activity may have played a role in the inability of SB203580 to effectively block proliferation of REPTC Potential signal blockers have to be examined thoroughly because ... much lower rate Both MK591 and SB203580 were able to block SEB-induced cell proliferation (Table 2) Effect of SEB and 5-LO inhibitor MK591 on human renal epithelial proximal tubular cells (REPTC)...

Ngày tải lên: 30/03/2014, 04:20

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Báo cáo sinh học: " Open Access Cloning of the canine RNA polymerase I promoter and establishment of reverse genetics for influenza A and B in MDCK cells" docx

... BamHI BamHI BamHI AvrII SpeI EcoRI SpeI HindIII SphI XbaI XbaI BamHI * XbaI BamHI BamHI SacI BamHI * EcoRI BamHI SacI BamHI BamHI AvrII 18S rRNA Gene MDCK Eco RI 7.1 kb Fragment B M C M Probe C C ... appropriate for cloning between the Bbs I sites of pAD4000 For influenza A strains, the restriction sites were BsmBI (PB1, PA, NP, M, and NS) or AarI (PB2, HA and NA) For influenza B strains, the restriction ... outbreak in humans [17] Conclusion Using the MDCK RNA pol I plasmid-based system, we have demonstrated the rescue of a wide variety of influenza A subtypes as well both lineages of influenza B...

Ngày tải lên: 18/06/2014, 18:20

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Báo cáo sinh học: " Improvement of cardiac contractile function by peptide-based inhibition of NF-B in the utrophin/dystrophin-deficient murine model of muscular dystrophy" potx

... pharmacodynamic efficacy of the NBD peptide in cardiac muscles of dko treated mice Both NF- B DNA binding activity, as well as nuclear levels of the p65 subunit of NF- B were elevated in the dko ... components of I B kinase (IKK) preventing association with the NF- B essential modulator (NEMO) regulatory subunit and prohibiting downstream NF- B signaling The NBD peptide blunted NF- B signaling, ... Pober JS, Ghosh S: Selective inhibition of NF- kappaB activation by a peptide that blocks the interaction of NEMO with the IkappaB kinase complex Science 2000, 289:1550-1554 Guttridge DC, Albanese...

Ngày tải lên: 18/06/2014, 19:20

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Báo cáo hóa học: "Urinary N-acetyl-beta -D-glucosaminidase and its isoenzymes A & B in workers exposed to cadmium at cadmium plating" pot

... was obtained from the subjects included in the study Body mass index Body mass index (BMI) is a measure of body fat based on height and weight of adult men and women The BMI was calculated by using ... stable (B) and heat labile (A) The amount of heat labile (A) was calculated by subtracting the heat stable (B) from the total NAG activity The levels of urinary total N-acetyl-beta -D-glucosaminidase ... the glomeruli and reabsorbed by the proximal tubules This complex can be broken down by lysosomes and releases unbound cadmium which can again induces the renal synthesis of metallothionein In...

Ngày tải lên: 20/06/2014, 00:20

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Báo cáo hóa học: " Cloning of the canine RNA polymerase I promoter and establishment of reverse genetics for influenza A and B in MDCK cells" pdf

... BamHI BamHI BamHI AvrII SpeI EcoRI SpeI HindIII SphI XbaI XbaI BamHI * XbaI BamHI BamHI SacI BamHI * EcoRI BamHI SacI BamHI BamHI AvrII 18S rRNA Gene MDCK Eco RI 7.1 kb Fragment B M C M Probe C C ... appropriate for cloning between the Bbs I sites of pAD4000 For influenza A strains, the restriction sites were BsmBI (PB1, PA, NP, M, and NS) or AarI (PB2, HA and NA) For influenza B strains, the restriction ... outbreak in humans [17] Conclusion Using the MDCK RNA pol I plasmid-based system, we have demonstrated the rescue of a wide variety of influenza A subtypes as well both lineages of influenza B...

Ngày tải lên: 20/06/2014, 01:20

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báo cáo hóa học:" Improvement of cardiac contractile function by peptide-based inhibition of NF-B in the utrophin/dystrophin-deficient murine model of muscular dystrophy" doc

... pharmacodynamic efficacy of the NBD peptide in cardiac muscles of dko treated mice Both NF- B DNA binding activity, as well as nuclear levels of the p65 subunit of NF- B were elevated in the dko ... components of I B kinase (IKK) preventing association with the NF- B essential modulator (NEMO) regulatory subunit and prohibiting downstream NF- B signaling The NBD peptide blunted NF- B signaling, ... Pober JS, Ghosh S: Selective inhibition of NF- kappaB activation by a peptide that blocks the interaction of NEMO with the IkappaB kinase complex Science 2000, 289:1550-1554 Guttridge DC, Albanese...

Ngày tải lên: 20/06/2014, 03:20

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báo cáo hóa học:" Substitutions in the Reverse Transcriptase and Protease Genes of HIV-1 Subtype B in Untreated Individuals and Patients Treated With Antiretroviral Drugs" potx

... G G → C Number of Substitutions http://www.jiasociety.org/content/7/1/69 Substitutions Figure of nucleotide substitutions in responsible for October 2003 ance mutations as defined by a IAS-USART ... responsible for PR in treated mutations of defined by a IAS-USA consensus panel, October individuals,as nucleotide substitutions in RT and resistance Numbers Numbers of nucleotide substitutions ... cause of GA hypermutation may involve a cellular factor, APOBEC3G, a cytidine deaminase that converts cytosine to uracil The activity of APOBEC3G is inhibited by the Vif protein.[5,7,8] In the absence...

Ngày tải lên: 20/06/2014, 08:20

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