4nf kappa b and pancreatic cancer

Báo cáo y học: " Nontypeable Haemophilus influenzae induces COX-2 and PGE2 expression in lung epithelial cells via activation of p38 MAPK and NF-kappa B" pps

Ngày tải lên : 12/08/2014, 15:21

... was markedly inhibited not only by p38 MAPK inhibitor SB203580, but also by another p38 MAPK inhibitor SB202190, and NF- http://respiratory-research.com/content/9/1/16 kappa B inhibitor PDTC Moreover, ... shown in Fig 6A & 6B, NTHi-induced COX-2 mRNA expression was markedly inhibited by pre-treatment of NF -kappa B inhibitor PDTC and p38 MAPK inhibitors SB203580 and Page of (page number not for citation ... of p38 MAPK inhibitors SB203580 and SB202190 and NF -kappa B inhibitor PDTC on the NTHi-induced COX-2 mRNA expression and PGE2 production were measured by using RT-PCR analysis and ELISA assay,...

9
290
0

báo cáo hóa học: " Inhibition of NF-κB activation by 5-lipoxygenase inhibitors protects brain against injury in a rat model of focal cerebral ischemia" docx

Ngày tải lên : 19/06/2014, 22:20

... cerebral IR BW -B 70C demonstrated a neuroprotective role through inhibition of both 5-LOX and NF- B It is a potent and a selective inhibitor of 5-LOX in vitro and in vivo with a long half-life and ... neurological score and (D) Effect of BWB 70C on regional cerebral blood flow (CBF) Changes in CBF were not significantly different after ischemia between the untreated (vehicle) and treatment (BW -B 70C) ... the cytoplasm by inhibitory proteins known as NF- B inhibitors (IκBs) Upon stimulation, I B is phosphorylated by an upstream I B kinase (IKK), which leads to its ubiquitination and proteosomal...

13
474
0

Báo cáo y học: "κ Increased AP-1 and NF-κB activation and recruitment with the β combination of the proinflammatory cytokines IL-1β, tumor necrosis factor alpha and IL-17 in rheumatoid synoviocyte" ppt

Ngày tải lên : 09/08/2014, 01:23

... Hata K, Andoh A, Shimada M, Fujino S, Bamba S, Araki Y, Okuno T, Fujiyama Y, Bamba T: IL-17 stimulates inflammatory responses via NF-kappaB and MAP kinase pathways in human colonic myofibroblasts ... obtained from Eurobio (Les Ulis, France) Antihuman OPG monoclonal antibody MAB8051, recombinant human OPG/Fc chimera 805-OS and biotinylated goat IgG antihuman OPG antibody (BAF805) were obtained from ... with × PBS Cells were then permeabilized for in 0.1% Triton X-100/1 × PBS, followed by three washings Nonspecific binding was abolished by incubation for hour at room temperature in blockade...

9
414
0

Báo cáo y học: "The S100A8/A9 heterodimer amplifies proinflammatory cytokine production by macrophages via activation of nuclear factor kappa B and p38 mitogen-activated protein kinase in rheumatoid arthritis" potx

Ngày tải lên : 09/08/2014, 07:20

... NF- B protein binding was verified by inhibition and supershift experiments with unlabeled NF- B consensus or mutant oligonucleotides and anti-NF- B p65 antibody (b) Effects of p38 MAPK inhibition ... preincubated with µM of the cell-permeable MAPK inhibitors PD98059, SB202190, and SB203580 and the negative control SB202474 (Calbiochem, San Diego, CA, USA), or with or without µM of the inhibitors ... inhibition, monocytes were preincubated with the MAPK inhibitors (PD98059, SB202190, and SB203580) and the control SB202474, then stimulated for 30 minutes by 10 µM S100A8/A9, and nuclear proteins were...

12
644
0

Báo cáo y học: "Elevated expression of CD30 in adult T-cell leukemia cell lines: possible role in constitutive NF-κB activation" pps

Ngày tải lên : 13/08/2014, 09:21

... by inserting a BamHI – SfiI fragment of pGEMT kBL1 in the BamHI – NotI sites of pMX The BglII site of pMX kBL1 was destroyed by cutting by BglII, filling in by T4 polymerase, and self-ligation ... control antibodies The following antibodies were used: PE-labeled mouse anti-human CD4 and CD25, FITC-labeled anti CD3 and CD8 (BD Biosciences Pharmingen, San Diego, CA); and PE-labeled mouse ... determined by immunofluorescence staining with respective antibodies and flow cytometric analysis Abbreviations: PB, peripheral blood; LN, lymph node NF- B activating molecules, we obtained several...

12
402
0

Tài liệu Báo cáo khoa học: Autophagy inhibits reactive oxygen species-mediated apoptosis via activating p38-nuclear factor-kappa B survival pathways in oridonin-treated murine ﬁbrosarcoma L929 cells doc

Ngày tải lên : 18/02/2014, 13:20

... Louis, MO, USA) Rabbit polyclonal antibodies against Bax, Beclin 1, LC3, p38, p-p38, ERK, I-jB, p-I-jB, NF-jB and b- actin; mouse polyclonal antibodies against cytochrome c and p-ERK; and horseradish ... oridonin; A -b, c, 24 h for oridonin) Scale bar = lm (A-a ,b) and 0.5 lm (A-c) The cells were cultured with 50 lM oridonin for h (B- a), h (B- b), 12 h (B- c), 24 h (B- d) or 36 h (B- e), or co-incubated with ... NF-jB promoted oridonin-induced L929 cell autophagy Oridonin treatment led to a decreased inhibitor kappa B (I-jB) level, with the increase of phosphorylated I-jB (p-I-jB) and NF-jB levels being...

16
547
0

Tài liệu Báo cáo khoa học: Staphylococcal enterotoxin C1-induced pyrogenic cytokine production in human peripheral blood mononuclear cells is mediated by NADPH oxidase and nuclear factor-kappa B doc

Ngày tải lên : 19/02/2014, 00:20

... NF-jB p65, rabbit polyclonal NF-jB p65, goat and rabbit polyclonal NF-jB p50, and rabbit polyclonal p47phox were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA) Rabbit polyclonal IjB-a, ... NF-jB which may bind to its target genes to trigger the production of IL- 1b and IL-6 that is blocked by the NF-jB inhibitor (PDTC or SN-50) Moreover, the NF-jB binding ability is abrogated by binding ... NFkappa B activation by I kappa B alpha EMBO J 12, 4685–4695 19 Babior BM, Kipnes RS & Curnutte JT (1973) Biological defense mechanisms The production by leukocytes of superoxide, a potential bactericidal...

13
465
0

Báo cáo y học: "DNA-like class R inhibitory oligonucleotides (INH-ODNs) preferentially block autoantigen-induced B-cell and dendritic cell activation in vitro and autoantibody production in lupus-prone MRL-Faslpr/lpr mice in vivo" potx

Ngày tải lên : 09/08/2014, 14:21

... IFN-α mAb (22100-1; PBL Biomedical Laboratories, Piscataway, NJ, USA), rabbit anti-mouse IFN-α polyclonal antibody (32100-1; PBL Biomedical Laboratories), HRP-conjugated donkey anti-rabbit IgG ... Class AM14 B cells, and primary mouse (INH-ODNs) inhibit Toll-like receptor-7 backbone-dependent manner (DCs),R and B inhibitory oligonucleotides B cells in a sequence-independent but (TLR7)-dependent ... AM14 B cells, and primary mouse B cells in a sequence-independent but backbone-dependent manner RAW264.7 macrophages (a, b) , Flt3L-propagated bone marrow-derived DCs (c), AM14 B cells (d), and...

16
318
0

báo cáo khoa học: "HGF/c-Met related activation of b-catenin in hepatoblastoma" pps

Ngày tải lên : 10/08/2014, 10:21

... subtype, b- catenin and Y654 b- catenin IHC and CTNNB1 gene status of hepatoblastomas with mutations Case Number Histologic Type Histologic Subtype b- catenin Y654 -b- catenin CTNNB1 mutation CCRG9 ... treatment Antibodies to b- catenin and Y654- b- catenin were used to probe the blots Anti-TBP and anti- b- actin were used to ensure equal loading tumours also lacked mutations in the CTNNB1 gene [40] ... Total b- catenin expression was assessed by immunoblot of the nuclear and cytoplasmic fractions As expected the Huh-6 cell line bearing a CTNNB1 mutation expressed b- catenin in both nucleus and...

10
201
0

Báo cáo y học: "Early Inhibition of NF-κB activation and MMP-9 secretion by plasma of human volunteers after ingestion of maritime pine bark extract (Pycnogenol)" pot

Ngày tải lên : 11/08/2014, 08:21

... was observed in the number of viable cells in the NF- B experiments The number of viable monocytes was 1.49 ± 0.29 × 106 and 1.70 ± 0.20 × 106 after incubation with samples obtained before and ... vegetables, fruits and fruit juices or marmalades, tea, coffee, cocoa, wine and beer) blood samples were drawn to obtain basal values Subsequently, the volunteers took tablets containing 200 mg standardized ... protocol Determination of NF- B activation by ELISA Plasma samples obtained before and after Pycnogenol intake were diluted 1:1 with RPMI medium as described above and incubated with monocytes overnight...

6
313
0

Báo cáo y học: "Dysregulated apoptosis and NFκB expression in COPD subjects" pptx

Ngày tải lên : 12/08/2014, 14:20

... CE, Fabbri LM: Cellular and structural bases of chronic obstructive pulmonary disease Am J Respir Crit Care Med 2001, 163:1304-1309 Dibbert B, Weber M, Nikolaizik WH, Vogt P, Schoni MH, Blaser ... soluble Fas ligand and soluble Fas in patients with chronic obstructive pulmonary disease Respir Med 2000, 94:1215-1220 Kucharczak J, Simmons MJ, Fan Y, Gelinas C: To be, or not to be: NF-kappaB ... NF B is a heterodimer, usually made up of Rel family proteins, p65 (RelA) and p50 (NF B) subunits In unstimulated cells NF B is sequestered in the cytoplasm due to its binding to I B and I B ...

12
259
0

Báo cáo y học: "Potential role and mechanism of IFN-gamma inducible protein-10 on receptor activator of nuclear factor kappa-B ligand (RANKL) expression in rheumatoid arthritis" pptx

Ngày tải lên : 12/08/2014, 17:22

... membrane was subsequently washed and incubated with a peroxidase-labeled rabbit anti-goat IgG antibody for hours at room temperature and then incubated with an enhanced chemiluminescence substrate ... expression, we used PTX, a bacterial toxin that inhibits Gai activation by ADP-ribosylating Gai subunits, and the inhibition of Gai by PTX was confirmed by measuring CREB phosphorylation that was ... and to find which subunit of CXCR3 contributes to RANKL expression by CXCL10 Materials and methods Synovial fluid and sera Synovial fluid and sera were collected from 18 patients who had RA and...

8
176
0

Environmental and Social Impacts of Shrimp farming in Tam Giang lagoon, Vietnam-Local perception

Ngày tải lên : 02/11/2012, 10:15

... wild (Gräslund & Bengtsson, 2001) Today shrimp makes up only 3-4% of global aquaculture production by weight, but almost 15% by value (FAO 1999a cited by Rönnbäck, 2002) The black tiger shrimp, ... inland and to regulate the microclimate of densely inhabited and intensively cultivated coastal areas The lagoons also facilitate boat transport between towns and villages along their shores, and ... September to April, and the highest rainfall is usually in October On average, about six floods occur and up to four storms visit Thua-Thien Hue annually, mostly occurring in September and October...

130
696
2

B-to-B Marketing in 2009:Trends in Strategies and Spending

Ngày tải lên : 10/08/2013, 16:17

... N/A B B B B B B B B B 2008 (N=656) 91% 81% 72% 70% 64% 60% 55% 54% 46% 45% 44% 39% 36% 34% 34% 11% 10% 9% 8% Percentage Point Change N/A -3 -4 -2 -7 -7 -1 -13 -13 -8 -5 2 N/A -6 -8 -7 N/A In both ... half also have the same belief about Email (59%), and more than 40% anticipate that Webbased Events: Webcasts, Webinars (47%), Inside Sales (46%), Blogs (43%), and Open, Public Social Networks (41%) ... A /B 13 Not in a marketing role B- to -B Marketing in 2009: Trends in Strategies and Spending CURRENT MARKETING TACTICS AND BUDGET ALLOCATIONS 14 B- to -B Marketing in 2009: Trends in Strategies and...

38
337
1

ĐỀ THI KHẢO SÁT ĐẠI HỌC MÔN TOÁN KHỐI (A,B,D) - TRUNG TÂM LUYỆN THI _NGUYỄN TRUNG TRỰC

Ngày tải lên : 05/09/2013, 16:34

... CÂU VI : Cho tam giác cân ABC B có tung độ B khác -3.đỉnh A (-3,-3) đường tròn nội tiếp tam giác ABC có phương trình (x-1)2 + y2 =9 Tìm B CÂU VII: Cho ba điểm A(-1,-2,0) B( 3,1,2) C(1,0,1) mặt phẳng ... phẳng P cho b n điểm A ,B, C,D đồng phẳng Và b n điểm hình thang CÂU IX: Tìm số phức z biết z(1-2i) =(3+4i)(2-i)2 B I LÀM THÊM tinh tích fân sau : : d(x) ∫ (x31+1) 3 √1+x CHÚC CÁC EM LÀM B I TỐT ...

2
740
0

Tài liệu ĐỀ THI THỬ ĐH-CĐ 2010 MÔN TOÁN KHỐI A–B–D–V_TRUNG TÂM THÀNH ĐẠT(ĐỀ 02) pptx

Ngày tải lên : 23/12/2013, 08:17

... VMCNB MC MN MB 6 ã Vỡ D l trung im ca MC nờn d ( M ,(CNB)) = 2d (D ,(CNB)) ị VMCNB = 2VDCNB = VDCSB = VS ABCD ị VDPQCNB = VSABNPQ 7 VS ABCD ị VSABNPQ = VS ABCD ị = 12 12 VDPQCNB Cõu V: T gi ... Ax + By + Cz = (vi A2 + B + C ) ã Vỡ (P) ^ (Q) nờn: A + 1 .B + 1.C = C = - A - B (1) A + 2B - C ã d ( M ,( P )) = 2 A +B +C = ( A + B - C )2 = 2( A2 + B2 + C ) (2) B = (3) (4) ã T (3): B = ... c: B = 2p -2 ã Tớnh B = x - x dx = A + B ũ ã Tớnh A = = -2 Cõu IV: Gi P = MN ầ SD, Q = BM ầ AD ị P l trng tõm DSCM, Q l trung im ca MB ã VMDPQ VMCNB = MD MP MQ 1 = = ị VDPQCNB = VMCNB MC...

4
381
4

Tài liệu Báo cáo khoa học: Collagen I regulates matrix metalloproteinase-2 activation in osteosarcoma cells independent of S100A4 pdf

Ngày tải lên : 18/02/2014, 11:20

... mAb from R&D systems (Minneapolis, MN, USA), TIMP-2 and MT1-MMP rabbit polyclonal from Panomics (Redwood City, CA, USA), S100A4 rabbit polyclonal from Abcam (Cambridge, UK) and RECK mouse mAb ... viability Open bars denote pHb-1 cells and ﬁlled bars denote II-1 1b cells (n = 3) *P < 0.05 compared with cells attached to plastic S100A4 pHβ B II-1 1b pHβ pHβ II-1 1b Mr (kDa) 20 II-1 1b S100A4 Actin ... in pHb-1 and II-1 1b cells (A) Determination of S100A4 by western blotting of cell lysates from pHb-1 and II-1 1b cells attached to plastic, using a total protein concentration of 81 lgÆmL)1, and...

12
572
0

Tài liệu Báo cáo Y học: Granule-bound starch synthase I A major enzyme involved in the biogenesis of B-crystallites in starch granules ppt

Ngày tải lên : 22/02/2014, 07:20

... SA137C and PA55 as described by Sambrook et al [30] A cDNA clone (named CD142) with an insert of 1696 bp was isolated and fully sequenced on both strands and submitted to GenBank (accession number ... granulebound proteins are fully described in [18]) Before blotting proteins onto nitrocellulose membrane (Protean BAÒ, Schleicher & Schuell), the gels were incubated for 15 in a Western blot buffer ... NaCl, 0.015 M sodium citrate and · Denhardt’s is 0.2 gÆL)1 Ficoll 400, 0.2 gÆL)1 PVP40 and 0.2 gÆL)1 BSA Probes were radiolabelled by random primers method as described by supplier’s instruction...

11
556
0