4making sense of physical cues in the extracellular matrix mechanotransduction
Báo cáo sinh học: "Q&A: What can microfluidics do for stem-cell research" ppsx
Ngày tải lên :
06/08/2014, 19:21
... handling of hESCs is an important factor in their instability over time, and therefore automated techniques for passaging and expansion may be a method for overcoming the problem of karyotypic instability ... organization of the cells into phenotypically distinct layers In fact, engineers have successfully manipulated parts of embryoid bodies in different ways using microfluidics tools to alter distinct fates ... controlling medium inflow and outflow In other words, engineers can provide steady-state conditions for cells, as well as fast and predictable changes in the environment surrounding the cells Of particular...
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Báo cáo sinh học: "Acoel and platyhelminth models for stem-cell research" pptx
Ngày tải lên :
06/08/2014, 19:21
... system (a) The current view of animal phylogeny indicates that Platyhelminthes and Acoela represent distinct evolutionary lineages, with acoels representing the outgroup to the rest of the Bilateria ... but they fail to differentiate properly [9] Therefore, the primary function of piwi in these animals may not be in stem-cell self-renewal but rather in potentiating the proper differentiation of ... regulation In Schmidtea and Macrostomum, transcripts of piwi homologs are present not only in the germ line but also in the neoblasts, and gene knockdown by RNAi results in the eventual loss of both the...
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Báo cáo y học: "A full menu for stem-cell research" pps
Ngày tải lên :
14/08/2014, 14:21
... Encouraging results were obtained after 10 months of follow-up An international phase II trial including 300 patients is in progress and the first results of this will be available in 2006; the trial ... research will lead to insights in a variety of fields, such as embryology, cell-based therapy and the origin of cancer We look forward to seeing advances in the field, perhaps at the next EuroConference ... originate from the transformation of normal stem cells Work in Weissman’s laboratory has established the role of a leukemia stem cell in leukemia If the results hold true for other tumors, the...
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The search for stem cell in human breast milk
Ngày tải lên :
11/09/2015, 09:57
... systems with epithelial cells’ lining, studies have document the presence of the of the stem cell in the luminal discharges due to the proximity of the stem cell niche to their luminal cavities ... follow-up which would be invaluable in managing the initiation of breastfeeding, especially in Chapter Introduction mothers of sick infants as well as in sick mothers of well infants (Neville and ... in urine of breastfed infants suggest that HBM can stimulate the production of IgA in the recipient infant (Goldblum et al 1989; Prentice 1996) 1.3.3.2 Lactoferrin The amount of lactoferrin in...
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ENGINEERING THREE DIMENSIONAL CULTURE PLATFORMS FOR HUMAN ADIPOSE DERIVED STEM CELL THERAPY
Ngày tải lên :
09/09/2015, 11:18
... potential of this field is also evident from the increase in the number of registered clinical trials in the US which are underway The clinical trials in the field of tissue engineering and regenerative ... encapsulating the ADSC-GMs into injectable, in situ gelling collagen hydrogels Incorporation of GMs into collagen hydrogels varies the mechanical properties of the hydrogels and hence allows for tuning ... Along with inducing stem cells to differentiate into a certain lineage of the damaged tissue, the micro-architecture of 3D systems can also regulate the growth factor secretion profiles of the stem...
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Tài liệu Báo cáo khoa học: Properties of ecdysteroid receptors from diverse insect species in a heterologous cell culture system – a basis for screening novel insecticidal candidates docx
Ngày tải lên :
18/02/2014, 08:20
... stretch of 35–37 amino acids that lie just to the N-terminal side of the DBD (Fig 1C) The EcRA isoforms from the two species share a few similar motifs in the middle region of the A ⁄ B domain (Fig ... beginning at the first amino acid following the LdUSP DBD Both amplicons and the pVP16 vector were digested with EcoRI and HindIII restriction endonucleases Ligation of the products into the linearized ... discussions during the course of the work, and the members of each of the laboratories whose technical assistance contributed to the effort The authors acknowledge the kind gifts of pure nonsteroidal...
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Guidelines for Human Embryonic Stem Cell Research potx
Ngày tải lên :
06/03/2014, 15:20
... president of the National Academy of Engineering The Institute of Medicine was established in 1970 by the National Academy of Sciences to secure the services of eminent members of appropriate professions ... Engineering in providing services to the government, the public, and the scientific and engineering communities The Council is administered jointly by both Academies and the Institute of Medicine ... those of the donor of the oocyte The oocyte does provide a very small amount of genetic information in the mitochondria, the “energy factories” of the cell, but the genes in the nucleus are of overriding...
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Báo cáo khoa học: Polypyrimidine tract-binding protein is essential for early mouse development and embryonic stem cell proliferation potx
Ngày tải lên :
07/03/2014, 00:20
... both in the ICM and in the trophectoderm (Fig 2A) In contrast, the expression of Oct3 ⁄ and Cdx-2 was restricted exclusively to the ICM or the trophectoderm (Fig 2A) In order to investigate the ... thymidine block We released cells from the block and fixed them at the time points indicated in Fig 5C, and then analyzed the DNA contents of the cells by flow cytometry Up until h after the release, ... transcripts of nPTB, which is one paralog of PTB [34] We investigated whether the expression of nPTB was increased in Ptb) ⁄ ) ES cells (Fig S2) The level of nPTB in Ptb) ⁄ ) ES cells was higher than in...
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Báo cáo hóa học: " Modulation of the major histocompatibility complex by neural stem cell-derived neurotrophic factors used for regenerative therapy in a rat model of stroke" ppt
Ngày tải lên :
18/06/2014, 16:20
... assess the property of NSC in cultures and track the migration and homing of allogeneic BrdUlabeled NSC in the host brain In addition to the expression of the class I and II MHC, the activation of ... comparing them to the standard curve to determine the copy number The process of calculating threshold cycles, preparing a standard curve, and determining the copy number was performed using system ... relief in ischemic rats given the NSC transplant and the upsurge of NGF in vivo, suggesting an immuno-modulation of the class I MHC by NSC-derived neurotrophins in the microenvironment of the lesioned...
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Báo cáo hóa học: " Varicella-zoster virus ORF 58 gene is dispensable for viral replication in cell culture" pptx
Ngày tải lên :
20/06/2014, 01:20
... role of the ORF58 gene product in VZV infection In this study, the N terminus of ORF57 was deleted in the process of deleting ORF58, because the C terminus of ORF58 overlaps with the N terminus of ... Southern blotting using a fragment of the internal sequence of the ORF58 gene, the ORF62/71 gene, or the kanamycin-resistance gene as a probe (Fig 2) As shown in Figure 2, the signal for the ... Moffat J, Ku CC, Arvin AM: Differentiation of varicella-zoster virus ORF47 protein kinase and IE62 protein binding domains and their contributions to replication in human skin xenografts in the...
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Báo cáo hóa học: "Biocompatible micro-sized cell culture chamber for the detection of nanoparticle-induced IL8 promoter activity on a small cell population" pot
Ngày tải lên :
21/06/2014, 00:20
... allows quantification of the fluorescence intensity and thus the inflammatory status of single cells The determination of the effect of miniaturization on nanoparticle-induced inflammatory cell responses ... Quantification of pIL8-GFP induction For quantification of an induced inflammatory reaction, the reporter cells pIL8-GFP A549 were used The induction of the promoter is linked to the production of the inflammatory ... in the microcavity One advantage of the miniaturized method is the recording of the complete cell culture area in one image Hence, every individual cell response is involved in the assessment of...
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Aseptic Technique for Cell Culture pptx
Ngày tải lên :
11/07/2014, 21:20
... the disinfectant to stay in contact with the spill for 30 Drain the contents of the catch basin into a container suitable for final sterilization by autoclaving Visually inspect the working interior ... interior of the laminar cabinet for evidence of dried culture material or media, especially in the corners of the cabinet Clean the interior of the cabinet with a laboratory detergent, rinse with ... entering the container 13 Slowly pass the opening of the vessel over the top of (rather than through) a Bunsen burner flame to burn off any contaminating matter Be careful when flaming containers...
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Báo cáo y học: "Hematopoietic stem cell transplantation for systemic lupus erythematosus" ppsx
Ngày tải lên :
09/08/2014, 01:23
... Using the notion of immune balance, the conditioning regimen is not intended to destroy every immune cell, but rather is intended to be sufficient to restore immune ‘balance’ In practice, the ... comparison, the notion of immune balance leads to less intense regimens that are more easily tolerated and have less infection-related risk Whether one concept or the other is correct remains unclear There ... active disease requiring treatment) after a minimum of 3–6 consecutive months of cyclophosphamide [2–6] Both the Hopkins and the Northwestern protocols are based on dose escalation of cyclophosphamide...
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Báo cáo y học: "Stem cell transplantation for rheumatic autoimmune diseases" potx
Ngày tải lên :
09/08/2014, 10:23
... [61] On the other hand, in the absence of GVHD, an increase of the anti-inflammatory IL-10 is described which may promote the formation of T-regulatory cells Apart from the increase of IL-10 ... the aforementioned preclinical studies have been instrumental in demonstrating basic principles of HSCT in experimental AD, the heterogeneity of results obtained in different transplant settings ... resulted in the cure of induced AD, but not of genetically determined AD [10-12] In autologous HSCT, and to a lesser extent in allogeneic HSCT, the outcome depended on the stage of the disease at the...
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Báo cáo y học: "A mouse embryonic stem cell bank for inducible overexpression of human chromosome 21 genes." ppsx
Ngày tải lên :
09/08/2014, 20:22
... to increased dosage of a gene and the degree of a certain property of the encoded protein, called intrinsic disorder [25] The protein disorder is defined as the total number of amino acids included ... for providing the recombinant plasmid containing the cDNA of Olig2; Dr Groner for providing the recombinant plasmid containing the cDNA of Runx1; Dr Whitelaw for providing the recombinant plasmid ... in the EB3 cell line with the corresponding transgenic inducible clones (in the biological replicates) grown in the presence of Tc (0 hours of induction) Microarray data analysis In the cases of...
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Báo cáo y học: " Comparative analysis of cell culture and prediction algorithms for phenotyping of genetically diverse HIV-1 strains from Cameroon" pps
Ngày tải lên :
10/08/2014, 05:21
... from these regions to further substantiate these findings and to understand the complexity of the emerging diversity of HIV in this region of high diversity Finally, although higher frequencies of ... practical while also being labor-intensive and time consuming The present investigation was aimed at characterizing genetically diverse HIV-1 strains circulating in Cameroon in terms of co-receptor usage ... (3TC/d4T/NVP) or a combination of lamivudine(3TC)/ Stavudine(d4T) or lamivudine(3TC)/Nevirapine(NVP), we examined the pol sequences of their viral isolates for evidence of drug specific resistance...
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Báo cáo y học: "Allogeneic hematopoietic stem cell transplantation for acute leukemia with Gilbert’s syndrome" pptx
Ngày tải lên :
10/08/2014, 21:23
... were involved in the provision of clinical care of the patient and the collection of data and the review of the manuscript GPY: supplied the acquisition of data, analysis and interpretation of ... developed indirect hyperbilirubinemia during the course of their treatment No patients died from the liver dysfunction In our case, transaminase and bilirubin levels were almost normal during the conditioning ... Department of Hematology, Nanfang Hospital, Southern Medical University; Member of the Asian-Pacific Society of Hematology; Member of Blood Branch of the Chinese Medical Association; Member of the Chinese...
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báo cáo khoa học: "Current status of haploidentical stem cell transplantation for leukemia" pps
Ngày tải lên :
10/08/2014, 22:20
... respectively Their series studies highlighted the efficacy of partial TCD in preventing GVHD in mismatched transplantation and the potential of post-grafting immunomodulation in lowering the risk of graft ... reflection of the heterogeneity of patients being treated And due to the high incidence and severity of GVHD, the safest approach of DLI after haploidentical HSCT, including cell dose, time of infusion, ... achieved by TCD The results of the prominent trials in this regard are shown in Table 1, and are divided according to whether in- vitro TCD was used The different characters of the two categories...
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báo cáo khoa học: "Biomimetic poly(amidoamine) hydrogels as synthetic materials for cell culture" potx
Ngày tải lên :
11/08/2014, 00:22
... some of its analogues can interact with adhesion regulating proteins of the integrin family, and play a role in promoting cell adhesion and spreading, mimicking the effect of some ECM proteins ... mirrored in the graphs of Figure 5b), where the larger weight loss of AGMA1-75 is apparent The degradation is then consistent with a gradual breaking of inter-chain linkages The obtained results indicate ... performed in water, PBS and ethanol Figure shows the proportional swelling, calculated using Expression 2, versus the percentage of crosslinker contained in each of the two types of hydrogels in the...
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