... diagnosis of depression was computed as the quotient ofthe cases classified as depression by both ofthe measures applied and the number of cases classified as depression by either ofthe measure ... On the validity ofthe Beck Depression Inventory A review Psychopathology 1998, 31:160-168 Hojat M, Shapurian R, Mehryar AH: Psychometric propertiesof a Persian version ofthe short form ofthe ... were included in the study if they were in the 11 grade (to avoid the symptoms of depression due to educational stress of appearing for board examination in the 10th as well as the 12th grades),...
... 1999) The concentration of minerals in the ECM, the composition of proteoglycans, the molecular weight ofthe proteoglycans, the amount of bound water in the ECM, and the pH ofthe ECM control the ... improve the electrical functions ofthe ECM Therefore the composition and degree of toxicity ofthe ECM-glycocalyx interface will affect the electrical field and the flow of biocurrents in the ECM The ... filtering aspect ofthe ECM The ECM is a transit area forthe passage of nutrients from the bloodstream into thecells and for toxins released by thecells that pass through to the bloodstream...
... almost all ofthe staining of these bands (Fig 3A) The most abundant band in human immature dendriticcells was the one at 83 kDa which may be related to a predominant glycosylation form ofthe CB1 ... the RNA content and cDNA synthesis in the different preparations Furthermore, the PCR primers for glyceraldehyde3-phosphate dehydrogenase and FAAH were selected on the basis ofthe sequence of ... extent, 2-AG [35,36] Dendritic cells, derived from bone marrow stem cells, are the most potent antigen-presenting cellsofthe immune system They play a central role in the initiation of primary immune...
... writing ofthe manuscript FMM participated in designing the study and the writing ofthe manuscript DFS participated in designing the study, coordinating the study and the writing ofthe manuscript ... of maturing DCs revealed two distinct patterns: down-regulation of groups of miR at hours of maturation with sustained low expression throughout the rest of maturation and up-regulation of other ... thank the staff ofthe NIH, Clinical Center, Dowling Clinic, Department of Transfusion Medicine, Clinical Center, NIH for collecting thecells and the Cell Processing Laboratory, Department of Transfusion...
... expression of HLA class I molecules on the MSCs Several speakers addressed the issue ofthe role of soluble factors in the immunomodulatory propertiesof MSCs Human MSCs caused T-helper-1 cells to ... DS, Keating A; the International Society for Cellular Therapy: Clarification ofthe nomenclature for MSC: the International Society for Cellular Therapy position statement Cytotherapy 2005, 7:393-395 ... administration [53] They contribute to myofibres and to long-term persisting functional satellite cells; furthermore, they restore sarcolemmal dystrophin when they are injected into the muscles ofthe immunosuppressed...
... characteristic of leukemic cells were not observed in the bone marrow MSCs of those cases [11] Unfortunately, we could not obtain the karyotype ofthe original leukemic cells Therefore, the complex ... the presence of a number of vacuoles in the cytoplasm of differentiated HPB-AML-I cells (Figure 4G) In contrast to the undifferentiated cells (Figure 4H), the differentiated HPB-AML-I cells formed ... consequence ofthe secretion of humoral factors by neoplastic cells or the direct interaction with them It is therefore very interesting that HPB-AML-I, regardless of its HSC or MSC origin, maintains the...
... phenotype of DC compared between the roller bottles and static flask system The numbers in the dot plots indicate the percentage ofcells out ofthe total population of floating cells having the indicated ... floating cells were analyzed without gating and phenotype of DC compared between the roller bottles and static flask system The numbers in the dot plots indicate the percentage ofcells out ofthe ... population of floating cells having the indicated phenotype The results of one out of similar experiments are shown Page of 11 (page number not for citation purposes) Journal of Immune Based Therapies...
... time of heatsetting (air, solvent or water) and the tension applied to the substrates during heatsetting [11, 12] The essence of this paper is to determine the effect of time of heatsetting on the ... resilience [%] The specimen were clamped between two chucks each of 20cm long A constant force of 200g was applied by attachinga weight to the front chuck ofthe specimen When the test started, the back ... Figure Effect of time of heatsetting on the linearity of load extension in weft direction of knitted Ingeo™ Poly (lactic acid) Figure Mean effect of time of heatsetting on the linearity of load extension...
... without the concomitant appearance of other signals in other parts ofthe spectrum This result could be a consequence ofthe paramagnetic propertiesof Fe(II), or it might arise from the presence of ... Addition of Fe2+ induced the displacement of several resonances in the [1H,15N]-HSQC NMR spectrum of CyaY, but the most striking consequence ofthe addition was the total disappearance of specific ... induce a line ˚ broadening of 1–100 Hz in nuclei located within A ofthe binding site, depending on the molecular mass ofthe protein and the strength ofthe magnetic field The only exceptions are...
... in the apparent Km values for these substrates The interpretation of these results is complicated because the levels of uncoupling will be variable depending on the mutant and the nature ofthe ... binding In the case ofthe R258K mutant this may be allowed because of disordering ofthe lysine side-chain, as was previously observed forthe R258Q mutant (Fig 1) [17] In the case ofthe R258H ... of mutations appears to be similar regardless of whether the prime substrate was penicillin G or ampicillin Utilization of 2-oxoglutarate by the R258 mutants The level of 2-OG conversion in the...
... densities of 87.3 · 103 cells per well, 146 · 103 cells per well and 29.1 · 103 cells per well, respectively The number ofcells was determined as cells per area of well, as used in the cell growth ... However, the development of effective and safe agents forthe prevention and treatment of cancer remains inefficient and costly, and falls short ofthe requirements for primary prevention among the ... compounds of interest in the prevention of colon cancer In the present study, we investigated the relationship of different structural factors of procyanidins, such as the mean degree of polymerization...
... domain of PABPN1 consisting of amino acids 1–125 ofthe wild-type protein (N-WT) Because the aim of this study was to investigate the effect on the fibrillation propertiesofthe most extreme disease-associated ... shown), the simultaneous presence of both amorphous aggregates and fibrils hampered the analysis of fibril formation kinetics For this reason, fibril formation was analyzed with the N-terminal domain of ... considerably shorter in the case ofthe variant with the poly l-alanine extension [26] In this work, we further compare these two N-terminal fragments Differences on the level of fibril formation kinetics...
... appearance of isoforms of CD63 of 50 and 70 kDa, respectively Together, these data indicate that the 70 kDa isoform of CD63 is a result of post-translational modifications ofthe 34 kDa isoform ... Fig CD63 isoforms in immature and mature dendriticcells (A) Immature dendritic cells, mature dendriticcells (DC) and Mel JuSo cells incubated for 48 h in the absence or presence of IFN-c were ... maturation ofdendritic cells, the apparent molecular mass of each of these eight carbohydrate containing polypeptides was increased by 10 kDa (Fig 2B) In mature dendritic cells, the 34 kDa form of...
... observed by the concentration dependence ofthe conformational transition Relatively small variations of protein concentration also lead to an increase ofthe rates at which the conformational ... force for formation ofthe pathological neuronal intranuclear RNP inclusions observed in FXTAS patients, little is known about the factors which determine their formation and stability [36] The ... At 55 °C, the spectrum 1914 Fig Summary ofthe modular structure of FXR proteins (A) and ofthe constructs used in this study (B) using a SMART-like [41] representation NDF stands forthe N-terminal...
... in the affinity ofthe PLRP2 for micellar substrates, and probably allows the interaction ofthe enzyme with the substrate structure Whether the motion ofthe lid promotes the recognition ofthe ... 79 and resulting in the unmasking ofthe catalytic triad ofthe enzyme induced by the motion ofthe flap The accessibility ofthe active site can be tested using the ability of an organophosphate, ... ofthe PLRP2 subfamily The aim of our study was to investigate the contribution ofthe N-terminal and C-terminal domains to the particular behavior of hoPLRP2 The structural organization of the...
... required forthe cofactor inhibition of phosphorylation (data not shown) The molecular basis forthe negative modulatory effects ofthe pterin cofactor and dopamine binding to wt-hPAH and their ... dependent regulatory propertiesofthe pterin cofactor Moreover, the superposition ofthe ternary structure [21] revealed a similar orientation ofthe pterin cofactor as in the binary structures ... features of its hysteretic properties Thus, the structures ofthe binary and ternary complexes of hPAH have provided a detailed picture ofthe protein contacts involved in the binding of biopterin cofactor...
... such that the size of G,~ is not ft(c"), then the same is true forthe language Pn, contradicting Theorem I The reason is that the size of a grammar forthe inverse homomorphic image of a language ... polynomiaUy bigger than the size of a grammar forthe language itself The proof of this claim rests on inspection of one ofthe standard proofs, say Hopcroft and Ullman [5] The result is proved ... that the permutation language P,~ really has s counting Proof This proof is really just a generalization ofthe proof of Theorem It uses, however, the Q subsets in a way that the proof of Theorem...
... of these novel EPR signals to either the cofactor or the P-cluster has proven elusive due to the fact that both of these metal clusters present in the Fe-only nitrogenase are diamagnetic in the ... (Q-Sepharose) also forthe purification ofthe MoFe protein in the present study Determination of nitrogenase activity and protein content Forthe determination of nitrogenase activity the routine assay ... that the reducing agent was not entirely removed from FeFe protein preparations in view ofthe lability ofthe protein even in the presence of only trace amounts of oxygen [8] Forthe sake of direct...
... dilution in mL of 20 mm Tris ⁄ HCl buffer pH 7.8 The HNE molecules specifically interacting with the binding sites ofthe two OBP forms were then displaced by the addition of a large excess ofthe OBP ... Evaluation ofthe third hypothesis, the molecular basis of which is described above, is the aim of this study Here, we report the binding properties, chemical modification and chemical resistance of recombinant ... acidic residues) compared with the porcine form (8.3%) [12], and because of their spatial arrangement in the 3D structure ofthe proteins (see below) The functionality of these HNE chemically modified...
... Analysis ofthepropertiesof this region illuminated a unified mechanism forthe formation ofthe peptide bond, the translocation ofthe tRNA molecules and the entrance of nascent proteins into the ... respectively, thus maintaining the symmetrical requirements ofthe PTC It seems therefore that increasing the stability ofthe PTC core structure is the task ofthe second shell ofthe symmetry-related ... facilitate the release ofthe leaving group Translocation ofthe acceptor stems of both tRNA follows, freeing the space needed for binding ofthe next aminoacyl tRNA (Fig 4) so that the following synthetic...