4harnessing the distinct properties of skin dendritic cells for intradermal vaccination

... diagnosis of depression was computed as the quotient of the cases classified as depression by both of the measures applied and the number of cases classified as depression by either of the measure ... On the validity of the Beck Depression Inventory A review Psychopathology 1998, 31:160-168 Hojat M, Shapurian R, Mehryar AH: Psychometric properties of a Persian version of the short form of the ... were included in the study if they were in the 11 grade (to avoid the symptoms of depression due to educational stress of appearing for board examination in the 10th as well as the 12th grades),...

Ngày tải lên: 13/08/2014, 18:21

... 1999) The concentration of minerals in the ECM, the composition of proteoglycans, the molecular weight of the proteoglycans, the amount of bound water in the ECM, and the pH of the ECM control the ... improve the electrical functions of the ECM Therefore the composition and degree of toxicity of the ECM-glycocalyx interface will affect the electrical field and the flow of biocurrents in the ECM The ... filtering aspect of the ECM The ECM is a transit area for the passage of nutrients from the bloodstream into the cells and for toxins released by the cells that pass through to the bloodstream...

Ngày tải lên: 14/02/2014, 22:20

... almost all of the staining of these bands (Fig 3A) The most abundant band in human immature dendritic cells was the one at  83 kDa which may be related to a predominant glycosylation form of the CB1 ... the RNA content and cDNA synthesis in the different preparations Furthermore, the PCR primers for glyceraldehyde3-phosphate dehydrogenase and FAAH were selected on the basis of the sequence of ... extent, 2-AG [35,36] Dendritic cells, derived from bone marrow stem cells, are the most potent antigen-presenting cells of the immune system They play a central role in the initiation of primary immune...

Ngày tải lên: 22/02/2014, 07:20

... writing of the manuscript FMM participated in designing the study and the writing of the manuscript DFS participated in designing the study, coordinating the study and the writing of the manuscript ... of maturing DCs revealed two distinct patterns: down-regulation of groups of miR at hours of maturation with sustained low expression throughout the rest of maturation and up-regulation of other ... thank the staff of the NIH, Clinical Center, Dowling Clinic, Department of Transfusion Medicine, Clinical Center, NIH for collecting the cells and the Cell Processing Laboratory, Department of Transfusion...

Ngày tải lên: 18/06/2014, 16:20

... expression of HLA class I molecules on the MSCs Several speakers addressed the issue of the role of soluble factors in the immunomodulatory properties of MSCs Human MSCs caused T-helper-1 cells to ... DS, Keating A; the International Society for Cellular Therapy: Clarification of the nomenclature for MSC: the International Society for Cellular Therapy position statement Cytotherapy 2005, 7:393-395 ... administration [53] They contribute to myofibres and to long-term persisting functional satellite cells; furthermore, they restore sarcolemmal dystrophin when they are injected into the muscles of the immunosuppressed...

Ngày tải lên: 09/08/2014, 08:23

... characteristic of leukemic cells were not observed in the bone marrow MSCs of those cases [11] Unfortunately, we could not obtain the karyotype of the original leukemic cells Therefore, the complex ... the presence of a number of vacuoles in the cytoplasm of differentiated HPB-AML-I cells (Figure 4G) In contrast to the undifferentiated cells (Figure 4H), the differentiated HPB-AML-I cells formed ... consequence of the secretion of humoral factors by neoplastic cells or the direct interaction with them It is therefore very interesting that HPB-AML-I, regardless of its HSC or MSC origin, maintains the...

Ngày tải lên: 10/08/2014, 10:20

... phenotype of DC compared between the roller bottles and static flask system The numbers in the dot plots indicate the percentage of cells out of the total population of floating cells having the indicated ... floating cells were analyzed without gating and phenotype of DC compared between the roller bottles and static flask system The numbers in the dot plots indicate the percentage of cells out of the ... population of floating cells having the indicated phenotype The results of one out of similar experiments are shown Page of 11 (page number not for citation purposes) Journal of Immune Based Therapies...

Ngày tải lên: 11/08/2014, 10:23

... time of heatsetting (air, solvent or water) and the tension applied to the substrates during heatsetting [11, 12] The essence of this paper is to determine the effect of time of heatsetting on the ... resilience [%] The specimen were clamped between two chucks each of 20cm long A constant force of 200g was applied by attachinga weight to the front chuck of the specimen When the test started, the back ... Figure Effect of time of heatsetting on the linearity of load extension in weft direction of knitted Ingeo™ Poly (lactic acid) Figure Mean effect of time of heatsetting on the linearity of load extension...

Ngày tải lên: 05/09/2013, 16:10

... without the concomitant appearance of other signals in other parts of the spectrum This result could be a consequence of the paramagnetic properties of Fe(II), or it might arise from the presence of ... Addition of Fe2+ induced the displacement of several resonances in the [1H,15N]-HSQC NMR spectrum of CyaY, but the most striking consequence of the addition was the total disappearance of specific ... induce a line ˚ broadening of 1–100 Hz in nuclei located within A of the binding site, depending on the molecular mass of the protein and the strength of the magnetic field The only exceptions are...

Ngày tải lên: 18/02/2014, 16:20

... in the apparent Km values for these substrates The interpretation of these results is complicated because the levels of uncoupling will be variable depending on the mutant and the nature of the ... binding In the case of the R258K mutant this may be allowed because of disordering of the lysine side-chain, as was previously observed for the R258Q mutant (Fig 1) [17] In the case of the R258H ... of mutations appears to be similar regardless of whether the prime substrate was penicillin G or ampicillin Utilization of 2-oxoglutarate by the R258 mutants The level of 2-OG conversion in the...

Ngày tải lên: 20/02/2014, 23:20

... densities of 87.3 · 103 cells per well, 146 · 103 cells per well and 29.1 · 103 cells per well, respectively The number of cells was determined as cells per area of well, as used in the cell growth ... However, the development of effective and safe agents for the prevention and treatment of cancer remains inefficient and costly, and falls short of the requirements for primary prevention among the ... compounds of interest in the prevention of colon cancer In the present study, we investigated the relationship of different structural factors of procyanidins, such as the mean degree of polymerization...

Ngày tải lên: 07/03/2014, 05:20

... domain of PABPN1 consisting of amino acids 1–125 of the wild-type protein (N-WT) Because the aim of this study was to investigate the effect on the fibrillation properties of the most extreme disease-associated ... shown), the simultaneous presence of both amorphous aggregates and fibrils hampered the analysis of fibril formation kinetics For this reason, fibril formation was analyzed with the N-terminal domain of ... considerably shorter in the case of the variant with the poly l-alanine extension [26] In this work, we further compare these two N-terminal fragments Differences on the level of fibril formation kinetics...

Ngày tải lên: 07/03/2014, 11:20

... appearance of isoforms of CD63 of  50 and  70 kDa, respectively Together, these data indicate that the 70 kDa isoform of CD63 is a result of post-translational modifications of the 34 kDa isoform ... Fig CD63 isoforms in immature and mature dendritic cells (A) Immature dendritic cells, mature dendritic cells (DC) and Mel JuSo cells incubated for 48 h in the absence or presence of IFN-c were ... maturation of dendritic cells, the apparent molecular mass of each of these eight carbohydrate containing polypeptides was increased by  10 kDa (Fig 2B) In mature dendritic cells, the 34 kDa form of...

Ngày tải lên: 08/03/2014, 02:20

... observed by the concentration dependence of the conformational transition Relatively small variations of protein concentration also lead to an increase of the rates at which the conformational ... force for formation of the pathological neuronal intranuclear RNP inclusions observed in FXTAS patients, little is known about the factors which determine their formation and stability [36] The ... At 55 °C, the spectrum 1914 Fig Summary of the modular structure of FXR proteins (A) and of the constructs used in this study (B) using a SMART-like [41] representation NDF stands for the N-terminal...

Ngày tải lên: 14/03/2014, 23:20

... in the affinity of the PLRP2 for micellar substrates, and probably allows the interaction of the enzyme with the substrate structure Whether the motion of the lid promotes the recognition of the ... 79 and resulting in the unmasking of the catalytic triad of the enzyme induced by the motion of the flap The accessibility of the active site can be tested using the ability of an organophosphate, ... of the PLRP2 subfamily The aim of our study was to investigate the contribution of the N-terminal and C-terminal domains to the particular behavior of hoPLRP2 The structural organization of the...

Ngày tải lên: 16/03/2014, 06:20

... required for the cofactor inhibition of phosphorylation (data not shown) The molecular basis for the negative modulatory effects of the pterin cofactor and dopamine binding to wt-hPAH and their ... dependent regulatory properties of the pterin cofactor Moreover, the superposition of the ternary structure [21] revealed a similar orientation of the pterin cofactor as in the binary structures ... features of its hysteretic properties Thus, the structures of the binary and ternary complexes of hPAH have provided a detailed picture of the protein contacts involved in the binding of biopterin cofactor...

Ngày tải lên: 17/03/2014, 09:20

... such that the size of G,~ is not ft(c"), then the same is true for the language Pn, contradicting Theorem I The reason is that the size of a grammar for the inverse homomorphic image of a language ... polynomiaUy bigger than the size of a grammar for the language itself The proof of this claim rests on inspection of one of the standard proofs, say Hopcroft and Ullman [5] The result is proved ... that the permutation language P,~ really has s counting Proof This proof is really just a generalization of the proof of Theorem It uses, however, the Q subsets in a way that the proof of Theorem...

Ngày tải lên: 17/03/2014, 20:20

... of these novel EPR signals to either the cofactor or the P-cluster has proven elusive due to the fact that both of these metal clusters present in the Fe-only nitrogenase are diamagnetic in the ... (Q-Sepharose) also for the purification of the MoFe protein in the present study Determination of nitrogenase activity and protein content For the determination of nitrogenase activity the routine assay ... that the reducing agent was not entirely removed from FeFe protein preparations in view of the lability of the protein even in the presence of only trace amounts of oxygen [8] For the sake of direct...

Ngày tải lên: 18/03/2014, 01:20

... dilution in mL of 20 mm Tris ⁄ HCl buffer pH 7.8 The HNE molecules specifically interacting with the binding sites of the two OBP forms were then displaced by the addition of a large excess of the OBP ... Evaluation of the third hypothesis, the molecular basis of which is described above, is the aim of this study Here, we report the binding properties, chemical modification and chemical resistance of recombinant ... acidic residues) compared with the porcine form (8.3%) [12], and because of their spatial arrangement in the 3D structure of the proteins (see below) The functionality of these HNE chemically modified...

Ngày tải lên: 23/03/2014, 10:20

... Analysis of the properties of this region illuminated a unified mechanism for the formation of the peptide bond, the translocation of the tRNA molecules and the entrance of nascent proteins into the ... respectively, thus maintaining the symmetrical requirements of the PTC It seems therefore that increasing the stability of the PTC core structure is the task of the second shell of the symmetry-related ... facilitate the release of the leaving group Translocation of the acceptor stems of both tRNA follows, freeing the space needed for binding of the next aminoacyl tRNA (Fig 4) so that the following synthetic...

Ngày tải lên: 23/03/2014, 17:22