4a role of the nucleosome barrier in stimulus selective gene induction
Báo cáo hóa học: " Differential inhibition of human cytomegalovirus (HCMV) by toll-like receptor ligands mediated by interferon-beta in human foreskin fibroblasts and cervical tissue" pdf
Ngày tải lên :
20/06/2014, 01:20
... production The effect of timing of TLR-ligand exposure on inhibition of HCMV infection production was next investigated using the concentration of each TLR-ligand that most effectively inhibited infection ... reduction in the number of infected cells, while at doses of μg/ml and 10 μg/ml of Poly I:C, >97% reduction in the number of infected cells was observed (Fig 1C) In contrast, pretreating HFF with ... directly investigated the effect of TLR ligand stimulation of cells in vitro on HCMV infection, Sainz et al [25] showed that the pretreatment of HFF with either IFN-α, IFNβ, or IFN-γ inhibited HCMV infection...
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Báo cáo y học: "Type I interferon receptor controls B-cell expression of nucleic acid-sensing Toll-like receptors and autoantibody production in a murine model of lupus" doc
Ngày tải lên :
09/08/2014, 14:22
... a critical role for the IFN-I signaling components IRF9 and STAT1 in murine B-cell expression of TLR7 as well as in the IFN-I-mediated induction of TLR9 expression [32] We examined the mRNA expression ... autoantibody profile and TLR-dependent B-cell response in SLE mice genetically deficient in the IFNAR2 chain of the IFNAR Autoantibody profiling using autoantigen microarrays in combination with ... characterized the role of the IFNAR2 chain of the IFNAR in the pristane model Pristane-treated IFNAR2-/- mice developed high titers of total serum IgM accompanied by significantly lower levels of the pathogenic...
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Báo cáo y học: " Nontypeable Haemophilus influenzae induces COX-2 and PGE2 expression in lung epithelial cells via activation of p38 MAPK and NF-kappa B" pps
Ngày tải lên :
12/08/2014, 15:21
... underlying NTHi-induced PGE2 expression and in the pathological analysis of the role of PGE2 in NTHi infection based on the findings from present study will bring us novel insights into the therapeutic ... that the activation of p38 MAPK and NF-kappa B is involved in the regulation of COX-2 and PGE2 in NTHi-infected lung epithelium In this study, we found the impact of p38 MAPK on the activation of ... about the patho-physiological role of the prostanoid intermediates including PGE2 in NTHi infections due to the absence of research information in this field Thus, even though we could not rule the...
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Báo cáo khoa học: Human lactoferrin activates NF-jB through the Toll-like receptor 4 pathway while it interferes with the lipopolysaccharide-stimulated TLR4 signaling potx
Ngày tải lên :
06/03/2014, 11:20
... resulting in the attenuation of LPS signaling As LF is known to interact with LPS, the binding of LPS with LBP may also be interfered with in the presence of hLF [9] Although the involvement of ... NF-jB induces several pro -in ammatory cytokines, such as TNF or IL-1b, whereas IRF3 induces interferon-b, thereby leading to the induction of interferon-inducible genes such as interferon-c-inducible ... affect the LPS-induced activation of NF-jB (Fig S4A, B) There is intense interest in the effects of breast-feeding on infants and the mechanisms behind these effects Compared with formula-fed infants,...
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Báo cáo khoa học: Phosphorylation of NF-jB proteins by cyclic GMP-dependent kinase A noncanonical pathway to NF-jB activation potx
Ngày tải lên :
08/03/2014, 02:20
... for induction of the faster migrating NF-jB band Note that the resting levels of NF-jB binding are relatively high in O3 cells, because the clone depends on the presence of interleukin-2 in the ... amounts of the competing PKG substrate peptide GRTGRRNSI (ÔPKI substrateÕ) (Fig 1C) We therefore set out to investigate the role of PKG in the induction of NF-jB Ó FEBS 2003 PKG increases the transactivating ... was measured in 40 lg (total protein) of lyzate The protein concentrations were determined by the BCA protein assay reagent kit (Pierce) As confirmation of protein expression, 20 lg of the same lyzates...
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Báo cáo y học: "Differential Constitutive and Cytokine-Modulated Expression of Human Toll-like Receptors in Primary Neutrophils, Monocytes, and Macrophages" docx
Ngày tải lên :
08/08/2014, 16:23
... either septic patients or controls, suggesting the possibility that increased expression of TLRs in this setting may be mediated via by pro-inflammatory cytokines rather than TLR-ligand binding ... by proinflammatory cytokines and increased responses to TLR ligands GM-CSF was shown to play an important role in inflammatory signaling in a model of LPS-induced lung injury Pretreatment of mice ... apoptosis soon after isolation After a 24-hour incubation, expression of TLR2 and TLR4 declined in neutrophils maintained in culture in the absence of cytokine supplementation Specifically, TLR2 and...
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Báo cáo y học: "No evidence of major effects in several Toll-like receptor gene polymorphisms in rheumatoid arthritis" pdf
Ngày tải lên :
09/08/2014, 01:22
... constitutes a noncoding mutation, probably at position -1486 of the promoting region but at position -851 of the gene (+1 being at the beginning of exon instead of exon 2) The primers used for ... (-13.8%) induce a T-helper type isotypic switch in B lymphocytes, which may be involved in the pathogenesis of RA Further evidence of the role for TLR9 comes from the efficacy in RA of chloroquine ... for the minor alleles since they are involved in presenting autoantigens to T cells, producing cytokines, and inducing ectopic architecture [38] Furthermore, there is strong evidence of a lack of...
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Báo cáo y học: "No evidence of major effects in several Toll-like receptor gene polymorphisms in rheumatoid arthritis" ppt
Ngày tải lên :
09/08/2014, 13:22
... constitutes a noncoding mutation, probably at position -1486 of the promoting region but at position -851 of the gene (+1 being at the beginning of exon instead of exon 2) The primers used for ... (-13.8%) induce a T-helper type isotypic switch in B lymphocytes, which may be involved in the pathogenesis of RA Further evidence of the role for TLR9 comes from the efficacy in RA of chloroquine ... for the minor alleles since they are involved in presenting autoantigens to T cells, producing cytokines, and inducing ectopic architecture [38] Furthermore, there is strong evidence of a lack of...
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Báo cáo y học: "The role of toll-like receptors in acute and chronic lung inflammation" pdf
Ngày tải lên :
11/08/2014, 03:20
... important roles in the induction of lung inflammation For example, the cytoplasmic NALP3 protein, a member of the NLR family that triggers assembly of the caspase-1 inflammasome and production of mature ... distinct role in the adaptive immune response to influenza through regulation of B-cell isotype switching [95,96] The role of TLR3 in the immune response to influenza has been debated in the ... of lung interstitial and intra-alveolar fibrosis and ALI [93] The role of TLRs has been intensively studied in influenza infection TLR7 expression on pDCs plays a cell-specific role against influenza...
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Báo cáo y học: "Early Characterization of Toll-like receptors in primary lung epithelial cells: strong impact of the TLR3 ligand poly(I:C) on the regulation of Toll-like receptors, adaptor proteins and inflammatory response" ppt
Ngày tải lên :
11/08/2014, 08:21
... provide further insight in the role of TLRs in the immune response of the lung epithelium to viral and bacterial infections and their contribution to virus and bacteria induced exacerbations of pulmonary ... of chemokines or cytokines found to be elevated by poly(I:C) stimulation including IL-6, IL-8, MIP-3α and GRO-α Interestingly, flagellin also induced IP-10 secretion in SAEC pointing to the induction ... stimulation of SAEC with the TLR3 ligand poly(I:C) resulted in the most pronounced inflammatory response regarding the secretion of cytokines and chemokines including an increased release of IL-6,...
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Báo cáo y học: " Differential expression of Toll-like receptors on human alveolar macrophages and autologous peripheral monocytes" pdf
Ngày tải lên :
12/08/2014, 14:20
... was introduced into the nose, throat and trachea with further instillation of 1% lidocaine to prevent coughing The bronchoscope was wedged into the right middle lobe or the lingula and 150 mL of ... subjects indicating a slower kinetic of TLR9 mRNA generation than in the case of TLR2 and TLR4 We also assessed the induction of cytokines following stimulation of the cells with TLR-specific ligands ... draft of the manuscript CN carried out the bronchoalveolar lavages ES participated in the preparation of the manuscript JJE participated in the preparation of the manuscript and was the PI of the...
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Báo cáo y học: "Trapped in a vicious loop: Toll-like receptors sustain the spontaneous cytokine production by rheumatoid synoviu" doc
Ngày tải lên :
12/08/2014, 15:22
... and anti -in ammatory functions of TLR2 may imply a dual role in Page of arthritis, depending on the cells and the processes involved However, the unambiguous proinflammatory function of TLR4 appears ... solid therapeutic target with consistent benefit In conclusion, the report by Nic An Ultaigh and colleagues supports the recently introduced concept of the involvement of TLRs in perpetuation of the ... the serotonin receptor antagonist mianserin, compounds suggested to inhibit TLR8 [12] So far, the only in vivo evidence supporting TLR involvement in the vicious in ammatory cycle in patients...
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Báo cáo y học: " Toll-like receptors in cellular subsets of human tonsil T cells: altered expression during recurrent tonsillitis" ppsx
Ngày tải lên :
12/08/2014, 16:20
... tems.com/pebiodocs/04303859.pdf The relative amounts of mRNA for the TLRs were determined by subtracting the Ct values for these genes with the Ct value for the housekeeping gene β-actin (∆Ct) [24] The amount of mRNA ... a role in the initial phase of the innate immune response However, the finding of TLRs in T cells might reflect a direct link to the adaptive immunity [28] This question has been addressed in ... within the GCs, but also in the adjacent T cell zones, whereas TLR2 and TLR3 expression was more intense in the T cell zones (Fig 3D-I) Although the staining of the TLRs does not discriminate...
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Báo cáo y học: " Science review: Key inflammatory and stress pathways in critical illness – the central role of the Toll-like receptors" ppsx
Ngày tải lên :
12/08/2014, 19:21
... domain containing receptors (in the case of IL-1) or TNF-family receptors (in the case of TNF) Hence, there is the potential for an amplification loop, dampened by feedback inhibition of the ... member of the peptidoglycan recognition protein family of proteins in the fly Imd itself is a homolog of the mammalian protein RIP, and the imd signaling pathway overall resembles the TNF signaling ... response, the investigator maintains close surveillance over innate immunity, measuring independent parts of the innate immune response or the global ability to defend against a specific type of infection...
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The role of downstream of kinase (DOK) 3 in toll like receptor signalling
Ngày tải lên :
09/09/2015, 10:16
... 2003) The protein structure of TRIF is mainly divided into its N-terminal domain containing binding sites for TRAF6 and TBK1, an intermediary TIR domain for binding to TLR3 and a C-terminus containing ... regulators of TLR signalling The study of TLR signalling has become increasingly complex with the surge in novel proteins being identified in recent years In addition, proteins that are involved in TLR ... were generated to study the physiological roles of these proteins in vivo but the controversy generated in findings using NOD1 knockout mice further added more complexity rather than understanding...
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báo cáo hóa học: " Toll-like receptors in cerebral ischemic inflammatory injury" pdf
Ngày tải lên :
19/06/2014, 22:20
... activation of the transcription factors NF-B, interferon-b promoter-binding protein (IRF)1, and IRF7, and generation of the pro-inflammatory cytokines IL-6 and TNF-a, among others [18] TLR3, on the other ... released into the extracellular space immediately after ischemic insult HMGB1 subsequently induced the release of inflammatory mediators in the post-ischemic brain [21] Intriguingly, regarding the ... glimpse into the relevance of TLR signaling in ischemic stroke, it has not enabled an understanding of the role of TLR signaling in specific cell types This issue is of great importance because the...
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Báo cáo y học: "Toll-like receptors and innate immune responses in systemic lupus" docx
Ngày tải lên :
09/08/2014, 10:21
... deleted of both TLR7 and TLR9 with provide additional insight into the potential therapeutic role of TLR9 inhibition in lupus Targeting type I interferons in systemic lupus erythematosus The parallel ... discoveries of the role of TLRs in stimulating type I IFNs, of pDCs as the primary source of this cytokine, and of the IFN-signature in lupus patients by microarray analyses [37,38] have identified the ... TLR activated but also on the cell type expressing the TLR In SLE the roles of TLR activation in B cells and DCs are the most evident; the effects of TLR activation in these cell types (autoantibody...
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Báo cáo y học: "Toll-like receptors and NOD-like receptors in rheumatic diseases" pdf
Ngày tải lên :
09/08/2014, 14:21
... highlight the role of innate immunity in the adaptive response, and the two responses are increasingly seen as inter-linked Rheumatoid arthritis There has been a longstanding hypothesis that infection ... a role in the initiation of RA (Figure 1) Molecules of microbial origin have been found in the joints of patients with RA [14,15], where they can trigger inflammatory reactions through PRRs These ... arthropathies are becoming clearer and they remain exciting therapeutic options One interesting example is in aseptic loosening that occurs in 10% of joint replacements, resulting in revision surgery...
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Báo cáo y học: " Gut flora enhance bacterial clearance in lung through toll-like receptors 4" docx
Ngày tải lên :
10/08/2014, 10:20
... reaction of lung against bacterial challenge The aim of this study is to investigate the role of gut flora in E.coli pneumoniainduced lung inflammation We hypothesized that commensal microflora in the ... effect of oral antibiotic pretreatment on NF-B DNA binding activity in the lung further corroborates the important role that commensal microflora play in inducing NF-B signaling in the lung ... not in C3H/HeJ mice To define the role of NF-B activation in the effect of oral antibiotic pretreatment on lung immunity, we examined the NF-B DNA binding activity of lung after E.coli intratracheal...
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