4 feasibility of industrial application of cga

... Colloidal gas aphrons (CGA) properties 10.3 Applications of CGA in the recovery of value-added food products 10.4 Feasibility of industrial application of CGA 10.5 Future ... composition of egg products 12.11 Concentration and stabilization of egg white and whole egg 12.12 Industrial extraction of egg-white proteins 12.13 Industrial extraction of yolk ... 1.5 Cascade of extractors for extraction of solid materials One of the major advantages of SFE processes is the fractionation of extracts Multi-step separation may be performed by use of several...

Ngày tải lên: 20/09/2015, 01:57

... accompanied by the release of cytochrome c from mitochondria, oligonucleosomal degradation of genomic DNA, activation of apoptotic caspases, and proteolytic cleavage of caspase substrates Although ... the functions of endogenous BIR family proteins by immunodepletion with specific antibodies The efficiency of depletion could not be accurately estimated because of the low contents of endogenous ... was not possible because of the low concentrations of the endogenous molecules As an alternative approach, each of four MBP fusions was added at lgÆmL)1 at the start of incubation, and the remaining...

Ngày tải lên: 30/03/2014, 16:20

... optimal temperature of 65 ◦ C and activity of 900 AGU/g protein An amyloglucosidase unit, AGU, is the amount of enzyme able to hydrolyze ␮mol of maltose per minute at 37 ◦ C and a pH of 4.3 Ethanol ... optimal pH 5.5, optimal temperature of 85 ◦ C and activity of 120 KNU-S/g enzyme A kilo novo unit, KNU-S, is the amount of enzyme that breaks down 5.26 g of starch per hour Glucoamylase cleaves ... extraction and hydrolysis processes Assessment of statistical significance was made at an ˛ value of 0.05 2.4 Extraction of anthocyanins and subsequent hydrolysis of ISP residues ISP roots were sliced...

Ngày tải lên: 05/05/2014, 08:39

... suspended in 0.2 ml of TM buffer Expression and purification of proteins VP51 and VP76 are the products of ORF wsv308 and wsv220 of WSSV (GeneBank accession no AF332093) and composed of 466 and 674 ... on immunofluorescence flow cytometry to study the localization of VP51 and VP76 by comparing the http://www.virologyj.com/content/3/1/76 mean fluorescence intensity of the population of intact ... better understanding of the molecular mechanism of WSSV infection and assembly, or be helpful for the control of virus infection in the future Conclusion The localization of the two proteins,...

Ngày tải lên: 20/06/2014, 01:20

... J, Yasumasu I and Yamagami K Cloning of cDNAs for the precursor protein of a low-molecular-weight subunit of the inner layer of the egg envelope (chorion) of the fish Oryzias-latipes Dev Biol ... synthesis of precursor proteins of the inner layer subunits under the influence of estrogen, at least in most species [16–19,51,66] Despite the confusing terminology used to designate this very Page of ... proteins of the inner layer of the envelope that surrounds the oocyte of the ovulated teleost egg We have used zona radiata proteins, a descriptive term, to designate these proteins because of the...

Ngày tải lên: 13/08/2014, 13:20

... first round of screening Half of the colony was streaked on SD/-Trp agar plate and the other half inoculated into 20µl of PCR mix PCR products were sequenced to determine the identity of the interacting ... Discussion 3.1 Principle of Split-Ubiquitin Screening System Studies have shown that the use of yeast two hybrid system, which is a genetic assay for in vivo detection of interactions of proteins, enables ... located at the C terminus of Cub will be cleaved off and result in downstream reactions, which is the activation of LacZ and HIS3 reporter genes [7] 3.2 Identification of Cellular Membrane Proteins...

Ngày tải lên: 02/11/2012, 11:08

... ratio of the UV-absorbing peak indicated it was composed mainly of nucleic acid In the profile of the NaOH-extracted polymer (B), the elution volume (80 ml) of saccharide corresponded to that of ... the elution profiles The profile of steaming-extracted polymer (A) contained two saccharide peaks (at elution volumes of 70 ml and 90 ml) and one UV-absorbing peak (at elution volume of 95 ml) The ... washing-extracted polymers contained large amounts of phosphorus (of 3.2% and 3.6%, respectively) Table shows molar ratios of the constituent neutral sugars of the polymers Although the molar ratio varied...

Ngày tải lên: 05/09/2013, 09:08

... 104 Activity 12.1: Risks of No Functional Specification Exercise 1: Creating an Origami Boat ! Create an origami boat Construct ... completing the above steps, you will discuss the activity with the class Activity 12.1: Risks of No Functional Specification 105 THIS PAGE INTENTIONALLY LEFT BLANK ...

Ngày tải lên: 24/01/2014, 10:20

... successful examples of binding-sequence discrimination, the native sequences of the RRM1 of Sex-lethal protein (1B7F_1) and KH1 domain of Poly C-binding protein-2 were both ranked first out of 256 sequences, ... universally conserved component of the core of the signal recognition particle (SRP) The structure of the complex (PDB code: 1HQ1) and the binding affinity of a series of RNA mutants have been determined ... tools capable of predicting the specificity of RNA-binding proteins across entire families (such as the RRM superfamily), or of redesigning the specificity of these proteins, would be of equal importance...

Ngày tải lên: 18/02/2014, 16:20

... 5Â-GCGAATTCGTTGAAAACTTTTAATTATCA GGAGAAAAC-3Â, 5Â-CGAGATCTAGCATGTCAGACG TGGAGTCTGGA-3Â and 5Â-GCGAATTCGCAACCAAA GACAACCTGGTTTTAATGTTTTGA-3Â, and 5Â-CGAG ATCTGAAATGAACGAGCTGCGTATGCCGAA-3Â and 5Â-GCGAATTCAACACAAGAGTTGTTTTATATTGAA ... suggesting the existence of some interactive cellular partner FRAP analysis of the nuage revealed the slow recovery of OlvasGFP (Fig 3B) When the whole part of the single compartment of nuage was bleached, ... processes of replacement of molecules in granules from other parts of the same granules or from the cytosol We performed a bleaching experiment in a whole part of a single compartment of the granule,...

Ngày tải lên: 18/02/2014, 16:20

... 2549 Study of the interactions of the Anp32a LRR module C de Chiara et al A B Fig Solution structure of the LRR domain of murine Anp32a (A) NMR bundle of the 10 best structures in terms of energy ... the presence of two extra N-terminal LRR motifs not observed in the crystal, and allows accurate definition of the C-terminal domain boundary Experimental Study of the interactions of the Anp32a ... different regions of the molecule As part of a long-term function-oriented structural effort aimed at understanding the molecular bases of polyQ disease proteins, we report here a study of the structural...

Ngày tải lên: 18/02/2014, 17:20

... C-terminal sequence of about 90 amino acids of unknown function The high degree of identity of the Cg-Clp1 and Cg-Clp2 Glyco_18 domains (84% identity) argues for a conservation of the tertiary structure ... additional 90 amino acid C-terminal sequence of unknown function (Fig 1) Hence, the overall structure of Cg-Clp2 is similar to that of Cg-Clp1 Expression of Cg-Clp2 transcripts during development ... Fig Multiple sequence alignment of Cg-Clp2 with members of the GH18 family (A) The predicted amino acid sequence of Cg-Clp2 is aligned with the amino acid sequence of three CLPs from the oyster...

Ngày tải lên: 19/02/2014, 00:20

... and different glycoforms of PrPC In this study, we have analyzed the glycoform patterns of PrPC of human, sheep, cattle and mice and compared them Variable immunoreactivity of anti-PrP antibodies ... (B) Signal intensities of the di- (d), mono- (j) and nonglycosylated isoform (m) of PrPC were quantified and calculated as percentages of the total signal The glycoforms of the protein bands were ... di-glycosylated isoform of PrPC than did antibodies to the structured core region However, the glycoform patterns of mouse PrPC always showed the highest signal intensity of the di-glycosylated isoform,...

Ngày tải lên: 19/02/2014, 02:20

... 103Q), a broad distribution of the size of the inclusions was noticed Moreover, the percentage of cells presenting inclusions as well as the distribution of the size of the inclusions were dependent ... percentage of the cell population with an FL2-H fluorescence greater than · 101 was recorded during the FACS analysis The percentage of decrease of DYm was calculated as the ratio of the percentage of ... scraped off the culture dish in NaCl ⁄ Pi Following centrifugation, pelleted cells were resuspended in mL of water An equivalent of mg of total cell protein was used per assay After addition of 500...

Ngày tải lên: 19/02/2014, 06:20

... of E coli Towards selection of stable proteins Despite the significant effort that has been made toward studying the stability of proteins, it is still not fully understood how the stability of ... Selection of folded proteins by phage-display (Eur J Biochem 271) 1611 Fig Effect of structural change on the selection (A) Structure of cyt b562 Residues M7 and H102 are the ligands of heme Heme ... bundle protein in the absence of any cofactors In this work, the authors used the method similar to that of Finucane et al [11] except that the protein A B-domain instead of His-tag was used to select...

Ngày tải lên: 19/02/2014, 12:20

... viscosity of the extract as a result of DNA, lL of DNase I (200 units) was added per 100 lL of buffer [30] Following filtration through two layers of miracloth and the addition of four volumes of ice-cold ... ÔStainsAllÕ analysis of Fig and the 1D immunoblotting of Fig As the full-length Dp427 isoform of dystrophin does not enter the second dimension of conventional 2D gels, the expression level of a-dystroglycan ... with antibodies to the Dp427 isoform of dystrophin (A), the a-subunit of the dystroglycan complex (a-DG; B), the Na+/K+ ATPase (NKA, C), the fast SERCA1 isoform of the sarcoplasmic reticulum Ca2+...

Ngày tải lên: 19/02/2014, 16:20

... study the effect of different parameter settings There are several parameters in the framework: the number of generated seeds r, the number of new candidates k2 and the number of selections k ... and Ryan McDonald 2008 A joint model of text and aspect ratings for sentiment summarization In Proceedings of the 46th Annual Meeting of the Association of Computational Linguistics: Human Language ... the process of improving the performance of a weak classifier by iteratively adding training data and retraining the classifier More specifically, bootstrapping starts with a small set of labeled...

Ngày tải lên: 19/02/2014, 19:20

... Statistics of the BioScope corpus The 2nd and 3d columns show the total number of cues within the datasets; the 4th and 5th columns show the percentage of negated and speculative sentences 70% of the ... children (starting from the root of the subtree) to the rule pattern subtree Nodes of type *scope* and * match any number of nodes, similar to the semantics of Regex Kleene star (*) Results As ... the rule tree; neighboring identical siblings of type *scope* or * are replaced by a single node of the corresponding type Figure shows an example of this transformation The rule tree pruning...

Ngày tải lên: 20/02/2014, 04:20

... 2003; Nakano and Hirai, 2004) formalized the task of extracting NEs as a chunking problem of a sequence of characters instead of a sequence of morphemes In this paper, we keep the naive formalization, ... morpheme m is a vector consisting of frequencies of all possible unigrams 126 The morpheme feature M F (mi ) is a pair of the surface string and the part -of- speech of mi The similar morpheme feature ... of NHK Corpus Nippon Housou Kyoukai (NHK) corpus is a set of transcriptions of 30 broadcast news programs which were broadcasted from June 1st 1996 to 12th Table shows the statistics of NEs of...

Ngày tải lên: 20/02/2014, 09:20

... angles of most of the residues of the Bowman±Birk inhibitor are distributed fairly evenly over the b- and PPII-regions of the Fig A MOLSCRIPT diagram [67] of the X-ray crystal structure of soybean ... recombinant human versions of synucleins, together with the A30P and A53T mutants of a-synuclein that cause familial cases of Parkinson's disease Unfortunately the quality of some of these synuclein ... beam, which prevented the acquisition of ROA data of suf®cient quality for reliable analysis A ROA spectrum of rather poor quality of an impure commercial sample of a-casein (composition unde®ned)...

Ngày tải lên: 21/02/2014, 15:20