4 therapeutic uses of human placental umbilical cord blood transfusion in neurology

Báo cáo y học: "Therapeutic potential of human umbilical cord mesenchymal stem cells in the treatment of rheumatoid arthritis" pps

Ngày tải lên : 12/08/2014, 15:22

... cells in vivo [38] The upregulation of IL-10 is in line with the induction of Tregs in vitro and in vivo in our study Moreover, in our experiments we did not find UC-MSCs in the joint of the ... differentiation of UC-MSCs staining for alkaline phosphatase Arrows indicate the accumulation of intracytoplasmic alkaline phosphatase of osteoblast Original magnification × 40 involved in the suppression of ... that administration of UC-MSCs attenuated systemic inflammation in CIA in mice UC-MSCs downregulated the production of the proinflammatory cytokines TNF-a, and IL-6 in vitro and in vivo In addition,...

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Báo cáo sinh học: " Human umbilical cord blood-derived mononuclear cell transplantation: case series of 30 subjects with Hereditary Ataxia'''' docx

Ngày tải lên : 18/06/2014, 19:20

... depending on the patient’s condition, within an interval of five to seven days Two ml of CSF was removed and replaced by ml of cell suspension during the intrathecal injection In terms of intravenous ... translating to functional disability include unsteadiness in walking, wide-based steps, inability to heel walk, unsteadiness in standing or sitting, dependence on a walking frame, walking aid ... in future studies Cord blood derived cells are being investigated in a myriad of preclinical disease models [18,19,24,25] The safety of CBMC transplantation has been investigated in several human...

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báo cáo hóa học:" Human umbilical cord blood-derived mononuclear cell transplantation: case series of 30 subjects with Hereditary Ataxia" pdf

Ngày tải lên : 20/06/2014, 03:20

... depending on the patient’s condition, within an interval of five to seven days Two ml of CSF was removed and replaced by ml of cell suspension during the intrathecal injection In terms of intravenous ... translating to functional disability include unsteadiness in walking, wide-based steps, inability to heel walk, unsteadiness in standing or sitting, dependence on a walking frame, walking aid ... in future studies Cord blood derived cells are being investigated in a myriad of preclinical disease models [18,19,24,25] The safety of CBMC transplantation has been investigated in several human...

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Báo cáo khoa học: "In vitro neuronal and osteogenic differentiation of mesenchymal stem cells from human umbilical cord blood" ppt

Ngày tải lên : 07/08/2014, 18:21

... llec gnimrofrep dna esu lacinilc rof SBF gnisu tuohtiw oviv xe sCSM dnapxe ot sdohtem dnif ot deen ew dna ,hcraeser ruo rof SBF desu ew ,yduts tneserp ni ,revewoH noitarefilorp sCSM rof noitidnoc ... 001-X notirT %4.0 neht dna ,nim rof enoteca %001 ,nim 01 rof lonahtem %001 dloc eci htiw detaert neht erew yehT erutarepmet moor ta nim 01 rof SBP ni edyhedlamrof %7.3 htiw dexif neht dna SBP ... noitcudni cinegoetso na desu ew ,sCSM eht morf noitaitnereffid cinegoetso ecudni oT laitnetop cinegoetso tibihxe sCSM doolb droC )A2 giF( nitnemiv dna 2HS snegitna detaler -myhcnesem rof ytivitisop...

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Báo cáo y học: " Intratracheal transplantation of human umbilical cord blood-derived mesenchymal stem cells attenuates " pps

Ngày tải lên : 12/08/2014, 13:22

... and sepsis induced by intratracheal instillation of E coli [16] We examined the effects of intratracheal delivery of human UCB-derived MSCs on survival, histology, and lung inflammation in mice ... graded on a five point scale: = minimal injury, = injury up to 25% of the field, = Page of 11 injury up to 50% of the field, = injury up to 75% of the field, and = diffuse injury [19] Myeloperoxidase ... protein macroarray analysis included inflammatory cytokines of interest, including complement 5a (C5a), the soluble form of intercellular adhesion molecule (sICAM)1, IL-1a, IL-1b, IL-6, IL-16, interferon-inducible...

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Báo cáo hóa học: "Safety evaluation of allogeneic umbilical cord blood mononuclear cell therapy for degenerative conditions" pot

Ngày tải lên : 18/06/2014, 16:20

... obtained from each patient before initiation of treatment Cell processing Umbilical Cord Blood (100~ 150 mL) was collected from healthy unrelated donors (signed an informed consent) in accordance ... Journal of Translational Medicine 2010, 8:75 http://www.translational-medicine.com/content/8/1/75 Page of Under the practice of medicine, several treatment facilities have been using cord blood ... guidelines for cord blood in each hospital After collection, each blood sample was tested for communicable diseases such as HBV, HCV, HIV, ALT, and Syphilis Cord blood was diluted with saline in...

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Báo cáo hóa học: "Future research and therapeutic applications of human stem cells: general, regulatory, and bioethical aspects" ppt

Ngày tải lên : 18/06/2014, 16:20

... treated by intravenous injection of ASCs [26-28] This immunosuppressive role of ASCs and their adjuvant effect in healing are also reflected in the encouraging results which are being achieved in various ... obtaining signed informed consent In this regard, risk-benefit assessment in this field is even more necessary in this field than in other areas because of the sometimes high underlying uncertainty ... different items, all of them necessary, including multiple surgical procedures, maintenance of strict aseptic conditions, specific training of technical staff and maintenance of overall technical...

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báo cáo hóa học:" BRCA1/2 genetic background-based therapeutic tailoring of human ovarian cancer: hope or reality?" ppt

Ngày tải lên : 20/06/2014, 07:20

... taken in account for the design of novel therapeutic strategies in this specific setting Clinical findings Preliminary clinical evidence appears in line with preclinical in vitro findings and indicates ... distinct domains: an amino-terminal DNAbinding domain (DBD), an auto-modification domain (AD), which is linked to BRCT-domain of BRCA1, and a carboxyl-terminal PARP homology domain, that includes ... mediating protein-protein interactions with phospho-proteins and the N-terminal RING domains is responsible for tight association of the two proteins This motif also confers E3-ubiquitin ligase...

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Báo cáo khoa học: "Transplantation of canine umbilical cord blood-derived mesenchymal stem cells in experimentally induced spinal cord injured dogs" pot

Ngày tải lên : 07/08/2014, 23:22

... ytisrevinU lanoitaN luoeS fo ’slaminA yrotarobaL fo esU dna eraC eht rof ediuG‘ eht fo senilediug eht ot gnidrocca demrofrep saw noitagitsevni sihT yduts yrujni droc lanips latnemirepxe eht rof desu ... erew sgod ehT ICS eht retfa keew a demrofrep saw sCSM-BCU eninac fo noitatnalpsnarT :FSCGhmr fo noitcejni dna sCSM-BCU eninac fo noitatnalpsnarT noitcejni eht rof noitulos enilas fo lµ 051 ni 601 ... ilumits cinegotim cificepsnon ro ralullec yb decudni noitarefilorp etycohpmyl-T sserppus sllec lamorts worram enob namuH MA innaiG ,S itnasirG ,P iccuettaM ,DP inognoL ,M isenaliM ,M ingaM ,C...

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Báo cáo khoa học: "Implantation of canine umbilical cord blood-derived mesenchymal stem cells mixed with beta-tricalcium phosphate enhances osteogenesis in bone defect model dogs" ppsx

Ngày tải lên : 07/08/2014, 23:22

... change of implant radiopacity Preparation of canine UCB-MSCs Fetal umbilical cord blood was collected during Caesarean section of pregnant female dogs Canine UCB-MSCs were produced by culturing ... weeks (Fig 2: proximal interface of C1) In group CM, continuity was established at three of six interfaces by 12 weeks (Fig 2: proximal interface of CM1, and 3) Histological findings Bone formation ... of canine umbilical cord blood- derived mesenchymal stem cells in experimentally induced spinal cord injured dogs J Vet Sci 2007, 8, 275-282 22 Lu J, Descamps M, Dejou J, Koubi G, Hardouin P,...

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Báo cáo khoa học: " Isolation and characterization of canine umbilical cord blood-derived mesenchymal stem cells" pps

Ngày tải lên : 07/08/2014, 23:22

... calcium mineralization Alizarin red S staining was used to determine the presence of calcium mineralization For Alizarin red S staining, cells were washed with D.W times and fixed in a solution of ... commonly observed in human umbilical cord blood derived MSCs [4] A rapid growth rate is an intrinsic aspect of cultured cUCB-MSCs [4] Cytotherapy using human umbilical cord blood stem cells frequently ... settings for the application of gene delivery were also investigated Materials and Methods Cell isolation and culture Canine umbilical cord blood (cUCB) and blood of the canine fetus heart using...

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Báo cáo y học: "Clinical observations on intensive immunosuppressive therapy combined with umbilical cord blood support for the treatment of severe aplastic anemia" ppt

Ngày tải lên : 10/08/2014, 21:23

... patients weighing over 80 kg were given double units of the umbilical cord blood transfusion HLA matching and blood typing were performed for umbilical cord blood selection, and cord blood units ... cyclosporine as treatment for severe acquired aplastic anemia Blood 1995, 85:3058-3065 Knudtzon S: In vitro growth of granulocyte colonies from circulating cells in human cord blood Blood 1974, ... A were combined with an unrelated umbilical cord blood transfusion The mononuclear cell (MNC) count of the transfused cord blood was 2.25-15.1 × 107 cells/kg with a median MNC count of 4.0 × 10...

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báo cáo khoa học: "Effect of anti-CD52 antibody alemtuzumab on ex-vivo culture of umbilical cord blood stem cells" doc

Ngày tải lên : 10/08/2014, 22:20

... trials now incorporate the expansion of http://www.jhoonline.org/content/1/1/19 one unit of cord blood following the infusion of another unexpanded unit in a double cord blood transplant setting The ... effect of alemtuzumab Further optimization with the use of this drug in other cytokine combinations in large-scale expansion cultures is being carried out, including the SCID-repopulating assay ... "stem" cell population in cord blood and improving the long-term hematopoietic reconstitution in cord blood transplantation [14,21] CD52 is a phosphatidylinositol-linked, 12-amino acid leukocyte...

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Báo cáo y học: "Antigenic presentation of heterologous epitopes engineered into the outer surface-exposed helix 4 loop region of human papillomavirus L1 capsomeres" potx

Ngày tải lên : 12/08/2014, 04:21

... use of baculovirus-derived L1 instead of prokaryotically expressed proteins; and structural constraints induced by the juxtaposition of several proline residues following internal h4 deletion In ... RC, Garcea RL: Expression of the human papillomavirus type 11 L1 capsid protein in Escherichia coli: characterization of protein domains involved in DNA binding and capsid assembly J Virol 1997, ... residues, including the prolines flanking both sides of h4 (aa 404–436; C-1, Figure 1) HPV type 16 L1 protein and its cognate cDNA were used for all L1 derivatives in this study [20] Into each of the...

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Báo cáo khoa học: "Antigenic presentation of heterologous epitopes engineered into the outer surface-exposed helix 4 loop region of human papillomavirus L1 capsomeres" docx

Ngày tải lên : 12/08/2014, 04:22

... use of baculovirus-derived L1 instead of prokaryotically expressed proteins; and structural constraints induced by the juxtaposition of several proline residues following internal h4 deletion In ... RC, Garcea RL: Expression of the human papillomavirus type 11 L1 capsid protein in Escherichia coli: characterization of protein domains involved in DNA binding and capsid assembly J Virol 1997, ... residues, including the prolines flanking both sides of h4 (aa 404–436; C-1, Figure 1) HPV type 16 L1 protein and its cognate cDNA were used for all L1 derivatives in this study [20] Into each of the...

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Báo cáo khoa học: Nuclear localization of human spermine oxidase isoforms – possible implications in drug response and disease etiology pptx

Ngày tải lên : 07/03/2014, 05:20

... microscopy of immunoﬂuorescent staining of SMO in cells overexpressing each of the isoforms provided direct visualization and conﬁrmation of protein localization 2800 without the possibility of contamination ... linked to the cytotoxicity Fig Catalysis of spermine oxidation by human spermine oxidase SMO catalyzes the cleavage of spermine, resulting in backconversion to spermidine, and the generation of ... al Cellular localization of human spermine oxidase A B C of human spermine oxidase in a human tumor cell line We also identify and classify SMO5 as a third human polyamine catabolic enzyme that...

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Báo cáo Y học: The a1b1 contact of human hemoglobin plays a key role in stabilizing the bound dioxygen Further evidence from the iron valency hybrids potx

Ngày tải lên : 31/03/2014, 15:20

... DISCUSSION In hemoglobin research, the central problem is understanding the cooperative binding of molecular oxygen to the a2b2 tetramer For human HbA, the a and b chains contain 141 and 146 amino-acid ... with increasing hydrogen ion concentration, involving a proton-catalysis by the distal (a58) histidine with pKa 6.2, as with the separated chains The value of ks also increased with increasing ... autoxidation rate of MbO2 or HbO2 Insofar as we have examined for more than a dozen of myoglobins, such a proton-catalyzed process could never be observed in the autoxidation of myoglobins lacking the...

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Báo cáo Y học: Kinetic studies of human tyrosyl-DNA phosphodiesterase, an enzyme in the topoisomerase I DNA repair pathway pot

Ngày tải lên : 31/03/2014, 21:21

... speciﬁc activity of TDP by following the cleavage of mM of substrate by 0.125 lM of enzyme in reaction buffer containing increasing concentrations of divalent ions The optimum pH was examined with 100 ... determined by the following procedure Initial velocities (v) were determined after ﬁtting the linear portion of the kinetic curve using MATLAB (The MathWorks Inc., Natick, MA, USA) The Lineweaver–Burk ... enzymatic activity of human TDP In contrast, in the presence of 0.1 mM Mn2+ a fourfold increase in human TDP activity was observed As the Mn2+ concentration increased, human TDP activity increased This...

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báo cáo hóa học:" Stem cells from umbilical cord blood do have myogenic potential, with and without differentiation induction in vitro" pdf

Ngày tải lên : 18/06/2014, 15:20

... complex of proteins (dystrophin-glycoprotein complex) responsible for the membrane maintenance of muscle cells A primary deficiency in any of these proteins induces to a secondary deficient of the ... states of myogenic progenitor cells [19] More recently, the in vivo myogenic differentiation of human umbilical cord blood was observed after the injection into the sjl dystrophic mice, suggesting ... mice, suggesting that human umbilical cord blood has myogenic precursors [20] Although the positive results of the in vivo injections, the interaction of these cells with human dystrophic muscle...

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Báo cáo sinh học: " Therapeutic effects of pyrrolidine dithiocarbamate on acute lung injury in rabbits" ppt

Ngày tải lên : 18/06/2014, 19:20

... Binding reactions were performed by adding μg of the nuclear extracts to a mixture containing 40 mol of biotin-labeled, double-stranded probes (5’AGTTGAGGGGACTTTCCCAGGC-3’) in 20 μl of binding ... with 1% protease inhibitor cocktail, containing antipain, aprotinin and leupeptin (500 μg, respectively), pepstatin (50 μg), bestatin (750 μg), phosphoramidone (400 μg), and trypsin inhibitor (500 ... experiment was terminated The lung tissues were embedded in paraffin wax, stained with hematoxylin and eosin, and examined under a light microscope The lung injury was scored according to inflammatory...

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